scholarly journals Staphylococcal infections among leukemic patients

2012 ◽  
Vol 9 (2) ◽  
pp. 270-276
Author(s):  
Baghdad Science Journal
Keyword(s):  
Staphylococcus Aureus ◽  
Clinical Specimen ◽  
Toxic Shock ◽  
Coagulase Negative Staphylococci ◽  
Hospital Acquired Infection ◽  
Microbiological Methods ◽  
Highly Sensitive ◽  
Superficial Wound ◽  
Hospital Acquired ◽  
Coagulase Positive Staphylococci

Staphylococcus are cause hospital community acquired infection and they are an important cause of health –care associated infection.The Coagulase positive Staphylococcus are Staphylococcus aureus which can implicated in toxic shock syndrome. Methicillin and Vancomycin Staphylococcus aureus resistant (MRSA, VRSA) become major cause of hospital- acquired infection and community acquired infection.Coagulase negative staphylococcus emerged as major cause of infection in immunocompromised patients.The main objective of this study was to evaluate the distribution of Staphylococci among leukemic patients since it is well known that leukemic patients are prone to be infected easily due to their immunosuppressed status.This study was undertaken between oct. 2009 and Jun 2010 at Iraqi center of hematology and medical genetics. 140 clinical specimen(aspirated wound,superficial wound,urine, blood) have deen collected carefully from leukemic patients and subjected to well known established microbiological methods for diagnosis and identification of the isolates .All isolates were tested for their susceptibility to antimicrobials according to Kirby –Bauer technique.Out of 140 clinical specimen collected from leukemic patients, it was possible to obtain( 63) bacterial isolates form which(43) of Coagulase negative staphylococci (CONS) and (20) of Coagulase positive staphylococci. Out of 43(CONS) isolates has been found that S.epidermidis constitutes (28)the highest of all isolates. Antimicrobial susceptibility reveald that S.aureus is highly sensitive to Gentamycin (85%), Erythromycin (80%), while it is resistant to the drugs Cefotaxim (45%), Choramphenicol(40%),and Tetracycline(20%). S.epidermidis show highly sensitive to Erythromycin(100%),Vancomycin (100%), and Cefotaxim(70%) and highly resistant to the drugs Chloromphenicol(45%), Augmentin(45%),Gentamycin (10%), and Tetracycline(10%).It is concluded that S.epidemidis rankes the first( 28)among the isolates and S.aureus ranke the 2nd .All isolates were highly resistant to Chloramphenicol and highly sensitive to Erythromycine.

scholarly journals Highly sensitive detection of Staphylococcus aureus by a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26824-26833 ◽  
Author(s):  
Ke Yang ◽  
Wenjing Yu ◽  
Guorong Huang ◽  
Jie Zhou ◽  
Xiang Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Hospital Acquired Infections ◽  
Food Borne ◽  
Highly Sensitive ◽  
Food Borne Illness ◽  
Highly Sensitive Detection ◽  
Hospital Acquired ◽  
The Impact

A highly sensitive method for detecting Staphylococcus aureus (S. aureus) is urgently needed to reduce the impact and spread of hospital-acquired infections and food-borne illness.

scholarly journals Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 734
Author(s):  
Wioleta Chajęcka-Wierzchowska ◽  
Joanna Gajewska ◽  
Patryk Wiśniewski ◽  
Anna Zadernowska
Keyword(s):  
Toxic Shock Syndrome ◽  
Food Poisoning ◽  
No Production ◽  
Toxic Shock ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcal Enterotoxins ◽  
Food Borne ◽  
Enterotoxin Genes ◽  
Toxic Shock Syndrome Toxin ◽  
Coagulase Positive Staphylococci

Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes responsible for the production of staphylococcal enterotoxins (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) in coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food from bars and restaurants. One hundred and eighteen CoNS strains were tested using polymerase chain reaction (PCR) to five superantigenic toxin genes, including five different types of classical enterotoxins (sea, seb, sec, sed and see) and the toxic shock syndrome toxin-1 (tsst-1) as well as to supertoxin-like genes. PCR-positive isolates were then tested using immunoenzymatic methods (SET-RPLA, Vidas SET 2) for toxin expression. Out of 118 CoNS strains, the presence of staphylococcal enterotoxins was confirmed in 72% of them. The most frequently found enterotoxin-like genotype was ser, selu. Two of the tested strains had up to ten different enterotoxin genes in the genome at the same time. Although no production of enterotoxins was detected in the CoNS, which means that their possible role in the epidemiology of food-borne diseases is minimal, the data demonstrated that the toxigenic capacity of the CoNS should not be ignored, and that this group of microorganisms should be continuously monitored in food.

scholarly journals Comparative Molecular Analysis of Community- or Hospital-Acquired Methicillin-Resistant Staphylococcus aureus

2003 ◽  
Vol 47 (1) ◽  
pp. 196-203 ◽  
Author(s):  
P. D. Fey ◽  
B. Saïd-Salim ◽  
M. E. Rupp ◽  
S. H. Hinrichs ◽  
D. J. Boxrud ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Relatedness ◽  
Health Concern ◽  
Toxic Shock ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Antibiotic Susceptibility Patterns ◽  
Mrsa Strains ◽  
Hospital Acquired ◽  
Susceptibility Patterns

ABSTRACT Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a growing public health concern that has been associated with pediatric fatalities. It is hypothesized that the evolution of CA-MRSA is a recent event due to the acquisition of mec DNA by previously methicillin-susceptible strains that circulated in the community. This study investigated the genetic relatedness between CA-MRSA, hospital-associated MRSA (HA-MRSA), and nonmenstrual toxic shock syndrome (nmTSS) isolates. Thirty-one of 32 CA-MRSA isolates were highly related as determined by pulsed-field gel electrophoresis and spa typing yet were distinguishable from 32 HA-MRSA strains. The 31 related CA-MRSA isolates produced either staphylococcal enterotoxin B (n = 5) or C (n = 26), and none made TSS toxin 1. All CA-MRSA isolates tested contained a type IV staphylococcal cassette chromosome mec (SCCmec) element. In comparison, none of the HA-MRSA isolates (n = 32) expressed the three superantigens. Antibiotic susceptibility patterns were different between the CA-MRSA and HA-MRSA isolates; CA-MRSA was typically resistant only to β-lactam antibiotics. Six of twenty-one nmTSS isolates were indistinguishable or highly related to the CA-MRSA isolates. MnCop, an nmTSS isolate obtained in Alabama in 1986, was highly related to the CA-MRSA isolates except that it did not contain an SCCmec element. These data suggest that CA-MRSA strains may represent a new acquisition of SCCmec DNA in a previously susceptible genetic background that was capable of causing nmTSS. CA-MRSA poses a serious health risk not only because it is resistant to the antibiotics of choice for community-acquired staphylococcal infections but also because of its ability to cause nmTSS via superantigen production.

scholarly journals Prevalence of Adhesion Genes fnbA & fnbB of Staphylococcus aureus Isolated from Dental Patients Attending Dentistry Department of Tehsil Headquarter Hospital Pasrur, Pakistan

2020 ◽  
Vol 1 (4) ◽  
Author(s):  
Muhammad Danish Mehmood ◽  
Huma Anwar Ul-Haq ◽  
Khushbu Farva ◽  
Zuhaib Farooq ◽  
Gul Muhammad Shaikh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Specific Primers ◽  
Dental Patients ◽  
Gram Staining ◽  
Microbiological Methods ◽  
Highly Sensitive ◽  
Genes Encoding ◽  
Pure Cultures ◽  
Polymerase Chain ◽  
Adhesive Molecules

Introduction: The present study was intended to isolate, characterize and investigate the prevalence of virulence genes encoding fibronectin (fnbA and fnbB) adhesive molecules of S. aureus from dental patients attending outpatient department of THQ Hospital Pasur. Methodology: A total of 100 oral samples were collected from dental patients, pure cultures was segregated and identified through conventional microbiological methods to evaluate the prevalence of S. aureus. Isolates were further characterized by using specific primers for genotype fnbA and fnbB. Results: Results of the study declared that 68 samples were positive (68%) for staphylococcus aureus on the basis of growth on selective media and appearance of typical colonies supported by gram staining. These gram positive staphylococci were positive (86.7%) in coagulase and catalase testing. The results of polymerase chain reaction (PCR) revealed that 47 (69.1%) and 23 (33.8%) isolates showed amplification with type specific primer 23Sr RNA and NUC gene respectively. Furthermore, fnbA and fnbB type specific genes of S. aureus did not show any amplification in PCR reaction. Conclusion: Irrespectively the data in the present study showed the prevalence of S. aureus is significantly high in males and of age group of 20-40 years but no positive result was found for prevalence of fnbA and fnbB genes. All the S. aureus isolates were highly sensitive to vancomycin, linezolid and clindamycin.

Nosocomial infections

2020 ◽  
pp. 669-673
Author(s):  
Ian C.J.W. Bowler ◽  
Matthew Scarborough
Keyword(s):  
Staphylococcus Aureus ◽  
Nosocomial Infections ◽  
Lower Respiratory Tract ◽  
Common Species ◽  
Coagulase Negative Staphylococci ◽  
Surgical Wounds ◽  
Organ System Failure ◽  
Considerable Morbidity ◽  
Hospital Acquired ◽  
Species Being

Hospital-acquired or nosocomial infections—defined for epidemiological purposes as infections manifesting more than 48 hours after hospital admission—are common. They affect 1.4 million people worldwide, involve between 5 and 25% of hospitalized patients at any one time and are associated with considerable morbidity, mortality, and cost. The most common sites of nosocomial infection are the urinary tract, surgical wounds, and the lower respiratory tract. Most are bacterial in origin, the most common species being Escherichia coli, Staphylococcus aureus (including methicillin-resistant Staphylococcus aureus), enterococci, Pseudomonas aeruginosa, and coagulase-negative staphylococci. The principal risk factors are extremes of age, the severity of underlying acute disease (e.g. neutropenia, organ system failure), and chronic medical conditions (especially diabetes, renal failure, and alcohol abuse).

scholarly journals Neonatal Septicemia: Isolation, Identification and Antibiotic Sensitivity Pattern of Bacteria in a Tertiary Hospital in Bangladesh

2017 ◽  
Vol 11 (2) ◽  
pp. 58-61
Author(s):  
Mahmuda Afrin ◽  
Md Abdullah Siddique ◽  
Abdullah Akhtar Ahmed ◽  
Md Nazrul Islam ◽  
Parimol Chandra Sarker ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Brain Heart Infusion ◽  
Antibiotic Sensitivity ◽  
Gram Positive ◽  
College Hospital ◽  
Cross Sectional ◽  
Neonatal Septicemia ◽  
Microbiological Methods ◽  
Sensitivity Pattern ◽  
Highly Sensitive

A cross sectional descriptive study was done to find out the causative organisms and their antibiotic sensitivities in the Department of Microbiology in collaboration with the Department of Paediatrics, Rajshahi Medical College Hospital (RMCH), Rajshahi during the period of July 2014 to June 2015. A total of 116 blood samples were taken aseptically from patients who were suspected of neonatal septicemia. Blood was then inoculated in Brain heart infusion broth. Bacterial isolation, identification and antimicrobial susceptibility testing were done by standard microbiological methods. Among 116 cases, 33(28.4%) were found to be culture positive. The most commonly isolated causative agents of neonatal septicemia were Staphylococcus aureus 17(51.5%) followed by Escherichia coli 10(30.3%), Streptococcus pneumoniae 03(9.09%), Klebsiella pneumoniae 02(6.06%) and Pseudomonas aeruginosa 01(3.03%). In general, all the Gram positive and Gram negative isolates were highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin. Gram positive bacteria were found to be particularly sensitive to vancomycin. They were moderately sensitive to ceftazidime followed in amoxicillin but were totally resistant to ampicillin. This study revealed that Staphylococcus aureus and E.coli are predominant causative organisms in neonatal septicemia and these are highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin.Faridpur Med. Coll. J. Jul 2016;11(2): 58-61

scholarly journals Toxigenicity in Staphylococcus aureus and Coagulase-Negative Staphylococci: Epidemiological and Molecular Aspects

2008 ◽  
Vol 1 ◽  
pp. MBI.S796 ◽  
Author(s):  
Cunha ◽  
R.A.O. Calsolari
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Toxic Shock ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcal Enterotoxins ◽  
Hospital Environments ◽  
Toxic Shock Syndrome Toxin ◽  
Molecular Aspects ◽  
Etiological Agents ◽  
General Aspects

Representatives of the Staphylococcus genus are the most common pathogens found in hospital environments, and they are etiological agents for a large variety of infections. Various virulence factors are responsible for the symptoms and severity of infections caused by Staphylococcus aureus. Among them are staphylococcal enterotoxins (SEs), which cause staphylococcal food poisoning, and toxic shock syndrome toxin-1 (TSST-1). Some reports indicate that TSST-1 and staphylococcal enterotoxins are also produced by coagulase-negative staphylococci (CNS). The present review aimed to discuss general aspects of staphylococcal toxins as well as the epidemiology, genetics and detection of toxins in Staphylococcus aureus and coagulase-negative staphylococci, since these microorganisms are becoming more and more frequent in nosocomial infections.

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