Using the Genetic Variation of Cycas taitungensis, an Endangered Island Cycad, to Evaluate Ex Situ Conservation Strategies

Author(s):  
Pei-Chun Liao ◽  
Li-Ping Ju ◽  
Ya-Zhu Ko ◽  
Mei-Hui Chen ◽  
Yu-Pin Cheng ◽  
...  
Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 934
Author(s):  
Chris O’Brien ◽  
Jayeni Hiti-Bandaralage ◽  
Raquel Folgado ◽  
Alice Hayward ◽  
Sean Lahmeyer ◽  
...  

Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.


2021 ◽  
Vol 10 (2) ◽  
pp. 128-130
Author(s):  
H.D.C. Soysa ◽  
U.S.B. Hettige ◽  
M.G. Cooray ◽  
D.R. Vidanapathirane

Nearly one in every five species of reptiles in the world is threatened with extinction and thus is conservation dependent. In order to develop effective and targeted species conservation strategies, detailed information on their distribution, systematics and ecology are necessary. Information such as the natural history and in-situ reproductive behaviors would be crucial for highly threatened species that require ex-situ conservation measures. Agamid lizards are a major component of the global reptile diversity and a significant number are threatened due to habitat loss and other threats such as smuggling.


Forests ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 469 ◽  
Author(s):  
Yanwen Deng ◽  
Tingting Liu ◽  
Yuqing Xie ◽  
Yaqing Wei ◽  
Zicai Xie ◽  
...  

Research Highlights: This study is the first to examine the genetic diversity of Michelia shiluensis (Magnoliaceae). High genetic diversity and low differentiation were detected in this species. Based on these results, we discuss feasible protection measures to provide a basis for the conservation and utilization of M. shiluensis. Background and Objectives: Michelia shiluensis is distributed in Hainan and Guangdong province, China. Due to human disturbance, the population has decreased sharply, and there is thus an urgent need to evaluate genetic variation within this species in order to identify an optimal conservation strategy. Materials and Methods: In this study, we used eight nuclear single sequence repeat (nSSR) markers and two chloroplast DNA (cpDNA) markers to assess the genetic diversity, population structure, and dynamics of 78 samples collected from six populations. Results: The results showed that the average observed heterozygosity (Ho), expected heterozygosity (He), and percentage of polymorphic loci (PPL) from nSSR markers in each population of M. shiluensis were 0.686, 0.718, and 97.92%, respectively. For cpDNA markers, the overall haplotype diversity (Hd) was 0.674, and the nucleotide diversity was 0.220. Analysis of markers showed that the genetic variation between populations was much lower based on nSSR than on cpDNA (10.18% and 77.56%, respectively, based on an analysis of molecular variance (AMOVA)). Analysis of the population structure based on the two markers shows that one of the populations (DL) is very different from the other five. Conclusions: High genetic diversity and low population differentiation of M. shiluensis might be the result of rich ancestral genetic variation. The current decline in population may therefore be due to human disturbance rather than to inbreeding or genetic drift. Management and conservation strategies should focus on maintaining the genetic diversity in situ, and on the cultivation of seedlings ex-situ for transplanting back to their original habitat.


Diversity ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 315 ◽  
Author(s):  
Carlos Mestanza-Ramón ◽  
Sujan M. Henkanaththegedara ◽  
Paola Vásconez Duchicela ◽  
Yadira Vargas Tierras ◽  
Maritza Sánchez Capa ◽  
...  

Biodiversity is vital for the stability of the planet; its ecosystem services provide essential elements for our survival and well-being. This review analyzes the national biodiversity policies and describes the main strategies for biodiversity conservation in Ecuador, one of the “mega-diverse” countries in the world with the highest species density. It deepens an analysis of in-situ and ex-situ conservation processes. Ecuador has six clear policies for biodiversity conservation. These policies strengthen biodiversity conservation through mechanisms that improve the well-being of wildlife by ensuring human, wildlife and ecosystem health. It promotes actions for the welfare of wildlife, through technical, administrative and legal tools. The National System of Protected Areas, with 60 protected areas, is the most effective in-situ conservation instrument at the country level. Several ex-situ conservation and management means for the conservation of wild species are being utilized, including nurseries, botanical gardens, zoos, germplasm banks, aquariums, species reproduction and rehabilitation centers. Ecuador is making slow progress on ex-situ conservation despite the availability of a sound policy framework, possibly due to financial, infrastructural, and/or technological challenges, and knowledge gaps. We propose fostering international research collaborations and establishing fully funded small-scale captive breeding programs at zoos, aquariums and university research facilities to help recovery of at-risk species of reptiles, amphibians, fish and species beyond Galapagos region. We recommend utilizing citizen science programs to fill the gaps of biodiversity information and increasing efforts to revive the ex-situ conservation strategies in protecting the unique biodiversity of Ecuador.


Author(s):  
Jayanthi Nadarajan ◽  
Karin van der Walt ◽  
Carlos A. Lehnebach ◽  
Hassan Saeiahagh ◽  
Ranjith Pathirana

2021 ◽  
Author(s):  
T S Santos ◽  
K Martins ◽  
A V Aguiar ◽  
M J Baptista Filho ◽  
J R Menucelli ◽  
...  

Abstract Dipteryx alata Vog. presents an excellent economic potential because of its multiple uses. However, it is currently threatened as a result of the degradation of the biome in which it occurs, the Brazilian Cerrado. Therefore, our goal was to provide subsidies for future breeding and ex situ conservation programs of D. alata. We evaluated genetic variation in growth, stem form, and wood quality traits in a progeny and provenance test planted in a random complete block design. The traits were measured in 37-year-old trees. Significant effect for provenance was obtained for all evaluated traits except bifurcation, but progeny effect was significant only for acoustic wave velocity (AWV). The estimates of individual narrow sense heritability were of low magnitude for all traits except AWV (0.24 ± 0.09). Eleven groups were formed based on Mahalanobis genetic distance with 83.1% of progenies allocated to one group. Dipteryx alata has genetic variability between progenies and provenances that can be explored to obtain genetic gain, especially for AWV. We also recommend the use of multi-trait selection index to obtain gains for both growth and wood quality traits. Controlled pollination between individuals from different progeny groups would increase variability in next generation breeding. Study Implications Dipteryx alata is a tree species that presents multiple uses and is threatened as a result of the devastation of the Brazilian Cerrado. We evaluated genetic variation in growth, stem form, and wood quality traits in a progeny and provenance test planted 37 years ago. We found genetic differences among progenies only for the wood quality trait, which can be explored to obtain genetic gains. The test is better suited for ex situ conservation, and the infusion of novel sources of germplasm is necessary for future generations of breeding.


2013 ◽  
Vol 41 (2) ◽  
pp. 348 ◽  
Author(s):  
Lucia DRAGHIA ◽  
Lăcrămioara IVĂNESCU ◽  
Elena-Liliana CHELARIU ◽  
Monica BOSCAIU ◽  
Nicoleta Luminița PARASCHIV

The current paper presents some characteristics of the anatomy of vegetative organs (adventive root and leaf) of Allium saxatile M. Bieb., a species rare in the Romanian flora, which have not been described before in the literature. Histo-anatomical studies performed on Allium saxatile plants sampled from three local populations identified in the Dobrogea area, complete already existing data from other species of this genus, and represent the beginning of future studies on ecological anatomy, taxonomy and ex situ conservation strategies. Some of the obtained results confirm data from literature, but additional observations have been made that show features not reported previously for any specie of the Allium genus, such as the presence of colenchymatized cells in the internal mesophyll at the lamina level; or the presence, at sheath level, of epidermal cells with uniformly lignified and thick walls.


2018 ◽  
Vol 65 (3-4) ◽  
pp. 121-128
Author(s):  
Einav Mayzlish-Gati ◽  
Margareta Walczak ◽  
Alon Singer ◽  
Tomer Faraj ◽  
Sivan Golan ◽  
...  

Israel is a geographically small and relatively new state (founded in 1948) with high population density, industrial development and economic growth, all of which negatively affect the environment, particularly biodiversity. There is, however, a growing awareness in the country of the need for environmental and biodiversity protection. The Israel Nature and Parks Authority (INPA), the official body legally responsible for protecting natural habitats, biodiversity and ecosystems in Israel, established a program to protect and conserve the endangered, near-threatened and very rare (ENtR) plants listed in the Israel Red Data Book of flora at risk. In this communication, we describe the ex-situ conservation strategies of the Israel Gene Bank (IGB) within this program. The IGB has expanded its role in the conservation of ENtR species from only ex-situ seed conservation to active involvement in whole-plant conservation and reintroducing ENtR species back into nature. In the past 10 years, 1289 accessions belonging to 68% of the species in the Red List were collected to form the IGB core collection of endangered and rare species. The germination unit of the IGB developed 198 new protocols and propagated 87 different ENtR species.


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