Determination of Sulfide with Acidic Permanganate Chemiluminescence for Development of Deep-sea in-situ Analyzers

Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L–L (luciferin–luciferase) assay with a miniature pumping unit and a photometry module for the measurement of the bioluminescence intensity. A heater and a temperature sensor were also included in the system to maintain an optimal temperature for the L–L reaction. In this study, the analyzer was evaluated in deep sea environments, reaching a depth of 200 m using a remotely operated underwater vehicle. We show that the ATP analyzer successfully operated in the deep-sea environment and accurately quantified total ATP within the concentration lower than 5 × 10−11 M.

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Re-evaluation assessment of a large volume sampling (LVS) system for the determination of petroleum hydrocarbons and their stable carbon isotopes in deep-sea waters

Analytical Methods ◽

10.1039/c9ay00983c ◽

2019 ◽

Vol 11 (31) ◽

pp. 3943-3957

Author(s):

Imma Tolosa ◽

Carlos Guitart ◽

Marianela Mesa-Albernas ◽

Carolina Funkey ◽

Roberto Cassi

Keyword(s):

Carbon Isotopes ◽

Large Volume ◽

Deep Sea ◽

Petroleum Hydrocarbons ◽

Isotope Ratio ◽

Stable Carbon Isotopes ◽

Sampling Technique ◽

Stable Carbon

A large volume sampling technique is used to develop and validate a home-made SPE system connected to a particle collector in situ pump for high sample pre-concentration and determination of petroleum hydrocarbons and their 13C/12C isotope ratio.

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INDIREKTE PAPIERCHROMATOGRAPHISCHE METHODE ZUR BESTIMMUNG DER HARNOESTROGENE

Acta Endocrinologica ◽

10.1530/acta.0.0380545 ◽

1961 ◽

Vol 38 (4) ◽

pp. 545-562 ◽

Cited By ~ 2

Author(s):

L. Kecskés ◽

F. Mutschler ◽

I. Glós ◽

E. Thán ◽

I. Farkas ◽

...

Keyword(s):

Pregnant Women ◽

Granulosa Cell ◽

Iron Content ◽

List Type ◽

Granulosa Cell Tumour ◽

Hydrolysis Of ◽

Phenol Fraction ◽

Qualitative Determination

ABSTRACT 1. An indirect paperchromatographic method is described for separating urinary oestrogens; this consists of the following steps: acidic hydrolysis, extraction with ether, dissociation of phenol-fractions with partition between the solvents. Previous purification of phenol fraction with the aid of paperchromatography. The elution of oestrogen containing fractions is followed by acetylation. Oestrogen acetate is isolated by re-chromatography. The chromatogram was developed after hydrolysis of the oestrogens 'in situ' on the paper. The quantity of oestrogens was determined indirectly, by means of an iron-reaction, after the elution of the iron content of the oestrogen spot, which was developed by the Jellinek-reaction. 2. The method described above is satisfactory for determining urinary oestrogen, 17β-oestradiol and oestriol, but could include 16-epioestriol and other oestrogenic metabolites. 3. The sensitivity of the method is 1.3–1.6 μg/24 hours. 4. The quantitative and qualitative determination of urinary oestrogens with the above mentioned method was performed in 50 pregnant and 9 non pregnant women, and also in 2 patients with granulosa cell tumour.

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In situ determination of toughness indices of fibre reinforced concrete

Materials and Structures ◽

10.1617/14157 ◽

2005 ◽

Author(s):

P. Antonaci

Keyword(s):

Reinforced Concrete ◽

Fibre Reinforced Concrete

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Development of a deep-sea mercury sensor using in situ anodic stripping voltammetry

GEOCHEMICAL JOURNAL ◽

10.2343/geochemj.2.0368 ◽

2015 ◽

Vol 49 (6) ◽

pp. 613-620 ◽

Cited By ~ 4

Author(s):

Masahiro Yamamoto ◽

Hitoshi Kodamatani ◽

Yuriko Kono ◽

Akinori Takeuchi ◽

Ken Takai ◽

...

Keyword(s):

Deep Sea ◽

Anodic Stripping Voltammetry ◽

Stripping Voltammetry ◽

Anodic Stripping ◽

Mercury Sensor

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In Situ Determination of the Increase in Saturation Solubility of Nanocrystals of Poorly Soluble Drugs

10.26226/morressier.57d6b2bdd462b8028d88d9ab ◽

2016 ◽

Author(s):

Roland Bodmeier

Keyword(s):

Poorly Soluble Drugs ◽

Saturation Solubility ◽

Poorly Soluble

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Single Crystal Growth of High-Pressure Phases of Ice and Salt Hydrate Far Below the Original Melting Point by Mixing Alcohol: Crystal Structure Determination of Magnesium Chloride Heptahydrate

10.26434/chemrxiv.11345063.v2 ◽

2020 ◽

Author(s):

Keishiro Yamashita ◽

Kazuki Komatsu ◽

Hiroyuki Kagi

Keyword(s):

Crystal Structure ◽

High Pressure ◽

Crystal Growth ◽

Single Crystal ◽

Room Temperature ◽

Growth Technique ◽

Salt Hydrate ◽

X Ray

An crystal-growth technique for single crystal x-ray structure analysis of high-pressure forms of hydrogen-bonded crystals is proposed. We used alcohol mixture (methanol: ethanol = 4:1 in volumetric ratio), which is a widely used pressure transmitting medium, inhibiting the nucleation and growth of unwanted crystals. In this paper, two kinds of single crystals which have not been obtained using a conventional experimental technique were obtained using this technique: ice VI at 1.99 GPa and MgCl<sub>2</sub>·7H<sub>2</sub>O at 2.50 GPa at room temperature. Here we first report the crystal structure of MgCl2·7H2O. This technique simultaneously meets the requirement of hydrostaticity for high-pressure experiments and has feasibility for further in-situ measurements.

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