scholarly journals Hydrogen sulfide induced by hydrogen peroxide mediates darkness-induced stomatal closure in Arabidopsis thaliana

2019 ◽  
Author(s):  
Yinli Ma ◽  
Luhan Shao ◽  
Jiao Niu
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Hydrogen Sulfide ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Stomatal Movement ◽  
Wild Type ◽  
Salicylhydroxamic Acid ◽  
Diphenylene Iodonium ◽  
Cysteine Desulfhydrase

Abstract Background Whether stomatal movement by darkness in Arabidopsis thaliana is mediated by hydrogen sulfide (H2S) is undiscovered yet, so the interaction between hydrogen peroxide (H2O2) and H2S in the process needs to be elucidated. Results Our results indicated that H2S modulators aminooxy acetic acid (AOA), potassium pyruvate (N3H3KO3) + ammonia (NH3), hydroxylamine (NH2OH), and hypotaurine (HT) inhibited darkness-induced stomatal closure, H2S generation and L-/D-cysteine desulfhydrase (L-/D-CDes) activity increased in wild-type A. thaliana leaves. Darkness induced stomatal closure in wild-type plants, but failed in Atl-cdes and Atd-cdes mutants. Additionally, both L-/D-CDes activity and H2S content were significantly decreased after applying H2O2 modulators salicylhydroxamic acid (SHAM), ascorbic acid (ASA), diphenylene iodonium (DPI), and catalase (CAT) in darkness, but there was almost no effects on H2O2 levels in the presence of AOA, C3H3KO3+NH3, NH2OH, and HT of wild-type plants in darkness. Moreover, darkness couldn't increase H2S content and L-/D-CDes activity of AtrbohF and AtrbohD/F mutants leaves, but increased H2O2 levels in Atl-cdes and Atd-cdes guard cells. Conclusions We observed that L-/D-CDes-generated H2S mediates stomatal closure by darkness, and functions downstream of H2O2 in A. thaliana.

scholarly journals Hydrogen sulfide induced by hydrogen peroxide mediates darkness-induced stomatal closure in Arabidopsis thaliana

2019 ◽  
Author(s):  
Yinli Ma ◽  
Luhan Shao ◽  
Jiao Niu
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Ascorbic Acid ◽  
Hydrogen Sulfide ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Wild Type ◽  
Activity Increase ◽  
Salicylhydroxamic Acid ◽  
Diphenylene Iodonium

Abstract Background Whether hydrogen sulfide (H2S) mediates darkness-induced stomatal closure in A. thaliana is unknown, and the interaction between H2S and hydrogen peroxide (H2O2) in the process needs to be elucidated. Results Our results indicated that H2S modulators hypotaurine (HT), aminooxy acetic acid (AOA), hydroxylamine (NH2OH) and potassium pyruvate (N3H3KO3)+ammonia (NH3) all inhibited darkness-induced stomatal closure, H2S generation and L-/D-cysteine desulfhydras (L-/D-CDes) activity increase in wild-type A. thaliana leaves. Darkness induced stomatal closure in wild-type plants, but failed in Atl-cdes and Atd-cdes mutants. Additionally, H2S content and L-/D-CDes activity were significantly decreased after application with H2O2 modulators ascorbic acid (ASA), catalase (CAT), diphenylene iodonium (DPI), and salicylhydroxamic acid (SHAM) in darkness, but there is almost no effects on H2O2 levels when in presence of HT, AOA, NH2OH, and C3H3KO3+NH3 in darkness in wild-type plants. Moreover, darkness couldn't increase H2S content and L-/D-CDes activity of AtrbohF and AtrbohD/F mutants leaves, but the levels of H2O2 increased in guard cells of Atl-cdes and Atd-cdes mutants. Conclusions The results suggest that L-/D-CDes-generated H2S mediates darkness-induced stomatal closure, and functions downstream of H2O2 in A. thaliana.

Hydrogen sulfide may function downstream of hydrogen peroxide in salt stress-induced stomatal closure in Vicia faba

2019 ◽  
Vol 46 (2) ◽  
pp. 136 ◽  
Author(s):  
Yinli Ma ◽  
Wei Zhang ◽  
Jiao Niu ◽  
Yu Ren ◽  
Fan Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Hydrogen Sulfide ◽  
Salt Stress ◽  
Vicia Faba ◽  
Laser Scanning ◽  
Stomatal Closure ◽  
Guard Cells ◽  
H2o2 Production ◽  
Diphenylene Iodonium ◽  
Cysteine Desulfhydrase

The roles of hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) in signalling transduction of stomatal closure induced by salt stress were examined by using pharmacological, spectrophotographic and laser scanning confocal microscopic (LSCM) approaches in Vicia faba L. Salt stress resulted in stomatal closure, and this effect was blocked by H2S modulators hypotaurine (HT), aminooxy acetic acid (AOA), hydroxylamine (NH2OH), potassium pyruvate (C3H3KO3) and ammonia (NH3) and H2O2 modulators ascorbic acid (ASA), catalase (CAT), diphenylene iodonium (DPI). Additionally, salt stress induced H2S generation and increased L-/D-cysteine desulfhydrase (L-/D-CDes, pyridoxalphosphate-dependent enzyme) activity in leaves, and caused H2O2 production in guard cells, and these effects were significantly suppressed by H2S modulators and H2O2 modulators respectively. Moreover, H2O2 modulators suppressed salt stress-induced increase of H2S levels and L-/D-CDes activity in leaves as well as stomatal closure of V. faba. However, H2S modulators had no effects on salt stress-induced H2O2 production in guard cells. Altogether, our data suggested that H2S and H2O2 probably are involved in salt stress-induced stomatal closure, and H2S may function downstream of H2O2 in salt stress-induced stomatal movement in V. faba.

Hydrogen sulfide induced by hydrogen peroxide mediates brassinosteroid-induced stomatal closure of Arabidopsis thaliana

2021 ◽  
Vol 48 (2) ◽  
pp. 195 ◽  
Author(s):  
Yinli Ma ◽  
Luhan Shao ◽  
Wei Zhang ◽  
Fengxi Zheng
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Hydrogen Sulfide ◽  
Stomatal Closure ◽  
Guard Cells ◽  
H2o2 Production ◽  
Wild Type ◽  
Synthesis Inhibitor ◽  
In The Wild ◽  
H2s Production

The role of hydrogen sulfide (H2S) and its relationship with hydrogen peroxide (H2O2) in brassinosteroid-induced stomatal closure in Arabidopsis thaliana (L.) Heynh. were investigated. In the present study, 2,4-epibrassinolide (EBR, a bioactive BR) induced stomatal closure in the wild type, the effects were inhibited by H2S scavenger and synthesis inhibitors, and H2O2 scavengers and synthesis inhibitor. However, EBR failed to close the stomata of mutants Atl-cdes, Atd-cdes, AtrbohF and AtrbohD/F. Additionally, EBR induced increase of L-/D-cysteine desulfhydrase (L-/D-CDes) activity, H2S production, and H2O2 production in the wild type, and the effects were inhibited by H2S scavenger and synthesis inhibitors, and H2O2 scavengers and synthesis inhibitor respectively. Furthermore, EBR increased H2O2 levels in the guard cells of AtrbohD mutant, but couldn’t raise H2O2 levels in the guard cells of AtrbohF and AtrbohD/F mutants. Next, scavengers and synthesis inhibitor of H2O2 could significantly inhibit EBR-induced rise of L-/D-CDes activity and H2S production in the wild type, but H2S scavenger and synthesis inhibitors failed to repress EBR-induced H2O2 production. EBR could increase H2O2 levels in the guard cells of Atl-cdes and Atd-cdes mutants, but EBR failed to induce increase of L-/D-CDes activity and H2S production in AtrbohF and AtrbohD/F mutants. Therefore, we conclude that H2S and H2O2 are involved in the signal transduction pathway of EBR-induced stomatal closure. Altogether, our data suggested that EBR induces AtrbohF-dependent H2O2 production and subsequent AtL-CDes-/AtD-CDes-catalysed H2S production, and finally closes stomata in A. thaliana.

Hydrogen sulfide may function downstream of hydrogen peroxide in mediating darkness-induced stomatal closure in Vicia faba

2018 ◽  
Vol 45 (5) ◽  
pp. 553 ◽  
Author(s):  
Yinli Ma ◽  
Jiao Niu ◽  
Wei Zhang ◽  
Xiang Wu
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Hydrogen Sulfide ◽  
Vicia Faba ◽  
Stomatal Closure ◽  
Guard Cells ◽  
H2o2 Accumulation ◽  
Vicia Faba L ◽  
Cysteine Desulfhydrase ◽  
The Relationship

The relationship between hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) during darkness-induced stomatal closure in Vicia faba L. was investigated by using pharmacological, spectrophotographic and lasers canning confocal microscopic approaches. Darkness-induced stomatal closure was inhibited by H2S scavenger hypotaurine (HT), H2S synthesis inhibitors aminooxy acetic acid (AOA) and hydroxylamine (NH2OH) and potassium pyruvate (N3H3KO3) and ammonia (NH3), which are the products of L-/D-cysteine desulfhydrase (L-/D-CDes). Moreover, darkness induced H2S generation and increased L-/D-CDes activity in leaves of V. faba. H2O2 scavenger and synthesis inhibitors suppressed darkness-induced increase of H2S levels and L-/D-CDes activity as well as stomatal closure in leaves of V. faba. However, H2S scavenger and synthesis inhibitors had no effect on darkness-induced H2O2 accumulation in guard cells of V. faba. From these data it can be deduced that H2S is involved in darkness-induced stomatal closure and acts downstream of H2O2 in V. faba.

scholarly journals Downregulating VAC14 in Guard Cells Causes Drought Hypersensitivity by Inhibiting Stomatal Closure

2020 ◽  
Vol 11 ◽  
Author(s):  
Zong-Qi Wang ◽  
Qi Liu ◽  
Ju-Hua Wu ◽  
Juan Li ◽  
Jun-Min He ◽  
...  
Keyword(s):  
Stomatal Closure ◽  
Guard Cells ◽  
Land Plant ◽  
Scaffolding Protein ◽  
Stomatal Movement ◽  
Wild Type ◽  
Physiological Factors ◽  
Surrounding Environment ◽  
Intracellular Activities

Stomata are a key land plant innovation that permit the regulation of gaseous exchanges between the plant interior and the surrounding environment. By opening or closing, stomata regulate transpiration of water though the plant; and these actions are coordinated with acquisition of CO2 for photosynthesis. Stomatal movement is controlled by various environmental and physiological factors and associates with multiple intracellular activities, among which the dynamic remodeling of vacuoles plays a crucial role. Phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2] is critical for dynamic remodeling of vacuoles. Its production requires a PI(3,5)P2-metabolizing complex consisting of FAB1/PIKfyve kinases, SAC phosphatases, and the scaffolding protein VAC14. Although genetic or pharmacological downregulation of PI(3,5)P2 causes hyposensitivity to ABA-induced stomatal closure, whether the effect of PI(3,5)P2 on stomatal movement is cell-autonomous and the physiological consequences of its reduction were unclear. We report that downregulating Arabidopsis VAC14 specifically in guard cells by artificial microRNAs (amiR-VAC14) results in enlarged guard cells and hyposensitivity to ABA- and dark-induced stomatal closure. Vacuolar fission during stomatal closure is compromised by downregulating VAC14 in guard cells. Exogenous application of PI(3,5)P2 rescued the amiR-VAC14 phenotype whereas PI(3,5)P2 inhibitor YM201636 caused wild-type plants to have inhibited stomatal closure. We further show that downregulating VAC14 specifically in guard cells impairs drought tolerance, suggestive of a key role of guard cell-produced PI(3,5)P2 in plant fitness.

scholarly journals Hydrogen sulfide induced by hydrogen peroxide mediates darkness-induced stomatal closure in Arabidopsis thaliana

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Yinli Ma ◽  
Liuxi Wang ◽  
Luhan Shao ◽  
Jiao Nio ◽  
Fengxi Zheng
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Hydrogen Sulfide ◽  
Stomatal Closure

Cytosolic alkalisation and nitric oxide production in UVB-induced stomatal closure in Arabidopsis thaliana

2014 ◽  
Vol 41 (8) ◽  
pp. 803 ◽  
Author(s):  
Xiao-Min Ge ◽  
Yan Zhu ◽  
Jun-Min He
Keyword(s):  
Nitric Oxide ◽  
Arabidopsis Thaliana ◽  
Laser Scanning ◽  
Stomatal Closure ◽  
Guard Cells ◽  
No Production ◽  
Uvb Radiation ◽  
Wild Type ◽  
Cytosolic Ph ◽  
No Scavengers

The role and the interrelationship of cytosolic alkalisation and nitric oxide (NO) in UVB-induced stomatal closure were investigated in Arabidopsis thaliana (L.) Heynh. by stomatal bioassay and laser-scanning confocal microscopy. In response to 0.5 W m–2 UVB radiation, the rise of NO levels in guard cells occurred after cytosolic alkalisation but preceded stomatal closure. UVB-induced NO production and stomatal closure were both inhibited by NO scavengers, nitrate reductase (NR) inhibitors and a Nia2–5/Nia1–2 mutation, and also by butyrate. Methylamine induced NO generation and stomatal closure in the wild-type but not in the Nia2–5/Nia1–2 mutant or wild-type plants pretreated with NO scavengers or NR inhibitors while enhancing the cytosolic pH in guard cells under light. NO generation in wild-type guard cells was largely induced after 60 min of UVB radiation. The defect in UVB-induced NO generation in Nia2–5/Nia1–2 guard cells did not affect the changes of guard cell pH before 60 min of UVB radiation, but prevented the UVB-induced cytosolic alkalisation after 60 min of radiation. Meanwhile, exogenous NO caused a marked rise of cytosolic pH in guard cells. Together, our results show that cytosolic alkalisation and NR-dependent NO production coordinately function in UVB signalling in A. thaliana guard cells.

Hydrogen peroxide is a common signal for darkness- and ABA-induced stomatal closure in Pisum sativum

2004 ◽  
Vol 31 (9) ◽  
pp. 913 ◽  
Author(s):  
Radhika Desikan ◽  
Man-Kim Cheung ◽  
Andrew Clarke ◽  
Sarah Golding ◽  
Moshe Sagi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Pisum Sativum ◽  
Nadph Oxidase ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Dependent Manner ◽  
Degenerate Oligonucleotide ◽  
Diphenylene Iodonium ◽  
Common Signal ◽  
Respiratory Burst Oxidase

The requirement for hydrogen peroxide (H2O2) generation and action during stomatal closure induced by darkness and abscisic acid (ABA) was investigated in pea (Pisum sativum L.). Stomatal closure induced by darkness or ABA was inhibited by the H2O2-scavenging enzyme catalase or the antioxidant N-acetyl cysteine (NAC), or by diphenylene iodonium (DPI), an inhibitor of the H2O2-generating enzyme NADPH oxidase. Exogenous H2O2 induced stomatal closure in a dose- and time-dependent manner, and H2O2 was also required for ABA-inhibition of stomatal opening in the light. H2O2 accumulation in guard cells was increased by darkness or ABA, as assessed with the fluorescent dye dichlorodihydrofluorescein diacetate (H2-DCFDA) and confocal microscopy. Such increases were inhibited by catalase, NAC or DPI, consistent with the effects of these compounds on stomatal apertures. Employing polymerase chain reaction (PCR) with degenerate oligonucleotide primers, several NADPH oxidase homologues were identified from pea genomic DNA that had substantial identity to the Arabidopsis thaliana (L.) Heynh. rboh (respiratory burst oxidase homologue) genes. Furthermore, an antibody raised against the tomato rboh identified immunoreactive proteins in epidermal, mesophyll and guard cells.

scholarly journals H 2 O 2 , Ca 2+ , and K +  in subsidiary cells of maize leaves are involved in regulatory signaling of stomatal movement

2019 ◽  
Author(s):  
Li Zhang ◽  
Yaqin Yao ◽  
Suiqi Zhang
Keyword(s):  
Zea Mays ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Stomatal Movement ◽  
Diphenylene Iodonium ◽  
Maize Leaves ◽  
Subsidiary Cells

Abstract Background : The stomata of maize ( Zea mays ) contain a pair of guard cells and a pair of subsidiary cells. To determine whether H 2 O 2 , Ca 2+ , and K + in subsidiary cells were involved in stomatal movement, we treated four-week-old maize (Zhengdan 958) leaves with H 2 O 2 , diphenylene iodonium (DPI), CaCl 2 , and LaCl 3 . Changes in content and distribution of H 2 O 2 , Ca 2+ , and K + during stomatal movement were observed. Results : When exogenous H 2 O 2 was applied, Ca 2+ increased and K + decreased in guard cells, while both ions increased in subsidiary cells, leading to stomatal closure. After DPI treatment, Ca 2+ decreased and K + increased in guard cells, but both Ca 2+ and K + decreased in subsidiary cells, resulting in open stomata. Exogenous CaCl 2 increased H 2 O 2 and reduced K + in guard cells, while significantly increasing them in subsidiary cells and causing stomatal closure. After LaCl 3 treatment, decreased and K + increased in guard cells, whereas both H 2 O 2 and K + decreased in subsidiary cells and stomata became open. Conclusions : These results indicated that H 2 O 2 and Ca 2+ were correlated positively with each other and with K + in subsidiary cells during stomatal movement. Both H 2 O 2 and Ca 2+ in subsidiary cells promote an inflow of K + , indirectly regulating stomatal closure.

Inhibition of abscisic acid-induced stomatal closure by ethylene is related to the change of hydrogen peroxide levels in guard cells in broad bean

2011 ◽  
Vol 59 (8) ◽  
pp. 781 ◽  
Author(s):  
XiGui Song ◽  
XiaoPing She ◽  
Juan Wang
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Abscisic Acid ◽  
Nadph Oxidase ◽  
Broad Bean ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Diphenylene Iodonium ◽  
Scavenging Enzymes ◽  
Exogenous H2o2

We analysed the role and relationship between hydrogen peroxide (H2O2) reduction and the inhibition of abscisic acid (ABA)-induced stomatal closure by ethylene. Like ascorbic acid (ASA), the most important reducing substrate for H2O2 removal, catalase, one of the H2O2 scavenging enzymes and diphenylene iodonium, an inhibitor of the H2O2-generating enzyme NADPH oxidase, both ethylene-releasing compound 2-chloroethylene phosphonic acid (ethephon, ETH) and 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene, were found to inhibit stomatal closure by ABA and to reduce H2O2 levels by ABA in guard cells, indicating that ethylene-caused inhibition of ABA-induced stomatal closure involves reduction of H2O2 levels in guard cells. Additionally, similar to ASA and catalase, ACC/ETH not only suppressed H2O2-induced stomatal closure and H2O2 levels in guard cells treated with exogenous H2O2 in light, but also reopened the stomata which had been closed by ABA and reduced H2O2 levels that had been generated by ABA. The abovementioned effects of ACC and ETH were dissimilar to that of diphenylene iodonium, an inhibitor of the H2O2-generating enzyme NADPH oxidase, which not only had incapability to reduce H2O2 levels by exogenous H2O2 but also could not abolish H2O2 that had been generated by ABA. So we suggest that ethylene probably induces H2O2 removal and reduces H2O2 levels in Vicia faba guard cells, and finally inhibits stomatal closure induced by ABA.

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