Exosomal microRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice

Abstract Background Gestational diabetes mellitus (GDM) is a pathological condition, affecting an increasing number of pregnant women worldwide. Safe and effective treatment for GDM is very important for the public health. In this study, we utilized a high-fat diet induced GDM model to evaluate the effects of LBP on GDM and examined the changes of exosomal microRNA expression profiling to decipher the potential underlying mechanism of LBP.Methods Female C57BL/6J mice were fed a control diet, HFD, or 150 mg/kg LBP- supplemented HFD for 6 weeks before conception and throughout gestation. Oral glucose tolerance test and plasma lipid levels were determined and liver histopathology was assessed. Sequencing was used to define the microRNA expression profiling of plasma exosomes in the three groups of mice and proteins expression levels of the candidate target genes were analyzed.Results LBP significantly relieved glucose intolerance, abnormal plasma lipid levels and pathomorphological changes of liver histopathology in HFD induced GDM mice. Moreover, we found that this effect of LBP was mediated by down-regulation of the increase of 6 miRNAs (miR-93-3p, miR-188-5p, miR-466k, miR-1188-5p, miR-7001-3p and miR-7115-5p) and reversing the increase protein expression of CPT1A, which is the target gene of miR-188-5p.Conclusions Our findings provide novel insights into the biological activities of LBP in the treatment of GDM.

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Exosomal MicroRNA Expression Profiling Analysis of the Effects of Lycium Barbarum Polysaccharide on Gestational Diabetes Mellitus Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/2953502 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12

Author(s):

Ya Xiao ◽

Weihao Chen ◽

Ruixue Chen ◽

Anling Luo ◽

Dayi Chen ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Protein Expression ◽

Gestational Diabetes Mellitus ◽

Expression Profiling ◽

Plasma Lipid ◽

Microrna Expression ◽

Lipid Levels ◽

Liver Histopathology ◽

Exosomal Microrna

Objective. Gestational diabetes mellitus (GDM) is a pathological condition, affecting an increasing number of pregnant women worldwide. Safe and effective treatment for GDM is very important for the public health. In this study, we utilized a high-fat diet-induced GDM model to evaluate the effects of LBP on GDM and examined the changes of exosomal microRNA expression profiling to decipher the potential underlying mechanism of LBP. Methods. Female C57BL/6J mice were fed a control diet, HFD, or 150 mg/kg LBP-supplemented HFD for 6 weeks before conception and throughout gestation. Oral glucose tolerance test and plasma lipid levels were determined, and liver histopathology was assessed. Sequencing was used to define the microRNA expression profiling of plasma exosomes in the three groups of mice, and protein expression levels of the candidate target genes were analyzed. Results. LBP significantly relieved glucose intolerance, abnormal plasma lipid levels, and pathomorphological changes of liver histopathology in HFD-induced GDM mice. Moreover, we found that this effect of LBP was mediated by downregulation of the increase of 6 miRNAs (miR-93-3p, miR-188-5p, miR-466k, miR-1188-5p, miR-7001-3p, and miR-7115-5p) and reversing the increase of the protein expression of CPT1A, which is the target gene of miR-188-5p. Conclusions. Our findings provide novel insights into the biological activities of LBP in the treatment of GDM.

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Early postpartum dyslipidemia and its potential predictors during pregnancy in women with a history of gestational diabetes mellitus

10.21203/rs.3.rs-52856/v2 ◽

2020 ◽

Author(s):

Ling Pei ◽

Huangmeng Xiao ◽

Fenghua Lai ◽

Zeting Li ◽

Zhuyu Li ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Maternal Age ◽

Operating Characteristic ◽

Characteristic Curve ◽

Lipid Levels ◽

Early Postpartum ◽

History Of ◽

Operating Characteristic Curve

Abstract Background: This study aimed to analyze the incidence of early postpartum dyslipidemia and its potential predictors in women with a history of gestational diabetes mellitus (GDM).Methods: This was a retrospective study. 589 women diagnosed with GDM were enrolled and followed up at 6–12 weeks after delivery. A 75g oral glucose tolerance test (OGTT) and lipid levels were performed during mid-trimester and the early postpartum period. Participants were divided into the normal lipid group and dyslipidemia group according to postpartum lipid levels. Demographic and metabolic parameters were analyzed. Multiple logistic regression was performed to analyze the potential predictors for early postpartum dyslipidemia. A receiver operating characteristic curve (ROC) was calculated to determine the cut-off values.Results: A total of 38.5% of the 589 women developed dyslipidemia in early postpartum and 60% of them had normal glucose metabolism. Delivery age, systolic blood pressure (SBP), glycated hemoglobin (HbA1c) and low-density lipoprotein cholesterol (LDL-C) were independent predictors of early postpartum dyslipidemia in women with a history of GDM. The cut-offs of maternal age, SBP, HbA1c values, and LDL-C levels were 35 years, 123mmHg, 5.1%, and 3.56 mmol/L, respectively. LDL-C achieved a balanced mix of high sensitivity (63.9%) and specificity (69.2%), with the highest area under the receiver operating characteristic curve (AUC) (0.696). When LDL-C was combined with age, SBP, and HbA1c, the AUC reached to 0.733.Conclusions: A lipid metabolism evaluation should be recommended in women with a history of GDM after delivery, particularly those with a maternal age > 35 years, SBP > 123 mmHg before labor, HbA1c value > 5.1%, or LDL-C levels > 3.56 mmol/L in the second trimester of pregnancy.

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Associations of lipid levels during gestation with hypertensive disorders of pregnancy and gestational diabetes mellitus: a prospective longitudinal cohort study

BMJ Open ◽

10.1136/bmjopen-2016-013509 ◽

2016 ◽

Vol 6 (12) ◽

pp. e013509 ◽

Cited By ~ 22

Author(s):

Hong Shen ◽

Xiaohua Liu ◽

Yan Chen ◽

Biwei HE ◽

Weiwei Cheng

Keyword(s):

Diabetes Mellitus ◽

Cohort Study ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Longitudinal Cohort Study ◽

Hypertensive Disorders Of Pregnancy ◽

Lipid Levels ◽

Hypertensive Disorders ◽

Prospective Longitudinal ◽

Prospective Longitudinal Cohort

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Profiling maternal plasma microRNA expression in early pregnancy to predict gestational diabetes mellitus

International Journal of Gynecology & Obstetrics ◽

10.1016/j.ijgo.2015.01.010 ◽

2015 ◽

Vol 130 (1) ◽

pp. 49-53 ◽

Cited By ~ 65

Author(s):

Yanan Zhu ◽

Fei Tian ◽

Hailing Li ◽

Youxia Zhou ◽

Jiafeng Lu ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Early Pregnancy ◽

Maternal Plasma ◽

Microrna Expression ◽

Plasma Microrna

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Association of Lipid Levels During Gestation With Preeclampsia and Gestational Diabetes Mellitus

Obstetric Anesthesia Digest ◽

10.1097/01.aoa.0000393165.62909.3b ◽

2011 ◽

Vol 31 (1) ◽

pp. 35

Author(s):

A. Wiznitzer ◽

A. Mayer ◽

V. Novack ◽

E. Sheiner ◽

H. Gilutz ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Lipid Levels

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Early postpartum dyslipidemia and its potential predictors during pregnancy in women with a history of gestational diabetes mellitus

Lipids in Health and Disease ◽

10.1186/s12944-020-01398-1 ◽

2020 ◽

Vol 19 (1) ◽

Author(s):

Ling Pei ◽

Huangmeng Xiao ◽

Fenghua Lai ◽

Zeting Li ◽

Zhuyu Li ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Maternal Age ◽

Operating Characteristic ◽

Characteristic Curve ◽

Lipid Levels ◽

Early Postpartum ◽

History Of ◽

Operating Characteristic Curve

Abstract Background This study aimed to analyze the incidence of early postpartum dyslipidemia and its potential predictors in women with a history of gestational diabetes mellitus (GDM). Methods This was a retrospective study. Five hundred eighty-nine women diagnosed with GDM were enrolled and followed up at 6–12 weeks after delivery. A 75 g oral glucose tolerance test (OGTT) and lipid levels were performed during mid-trimester and the early postpartum period. Participants were divided into the normal lipid group and dyslipidemia group according to postpartum lipid levels. Demographic and metabolic parameters were analyzed. Multiple logistic regression was performed to analyze the potential predictors for early postpartum dyslipidemia. A receiver operating characteristic curve (ROC) was calculated to determine the cut-off values. Results A total of 38.5% of the 589 women developed dyslipidemia in early postpartum and 60% of them had normal glucose metabolism. Delivery age, systolic blood pressure (SBP), glycated hemoglobin (HbA1c) and low-density lipoprotein cholesterol (LDL-C) were independent predictors of early postpartum dyslipidemia in women with a history of GDM. The cut-offs of maternal age, SBP, HbA1c values, and LDL-C levels were 35 years, 123 mmHg, 5.1%, and 3.56 mmol/L, respectively. LDL-C achieved a balanced mix of high sensitivity (63.9%) and specificity (69.2%), with the highest area under the receiver operating characteristic curve (AUC) (0.696). When LDL-C was combined with age, SBP, and HbA1c, the AUC reached to 0.733. Conclusions A lipid metabolism evaluation should be recommended in women with a history of GDM after delivery, particularly those with a maternal age > 35 years, SBP > 123 mmHg before labor, HbA1c value > 5.1%, or LDL-C levels > 3.56 mmol/L in the second trimester of pregnancy.

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Substantially Altered Expression Profile of Diabetes/Cardiovascular/Cerebrovascular Disease Associated microRNAs in Children Descending from Pregnancy Complicated by Gestational Diabetes Mellitus—One of Several Possible Reasons for an Increased Cardiovascular Risk

Cells ◽

10.3390/cells9061557 ◽

2020 ◽

Vol 9 (6) ◽

pp. 1557 ◽

Cited By ~ 1

Author(s):

Ilona Hromadnikova ◽

Katerina Kotlabova ◽

Lenka Dvorakova ◽

Ladislav Krofta ◽

Jan Sirc

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Expression Profile ◽

Heart Defects ◽

Cerebrovascular Diseases ◽

Clinical Findings ◽

Microrna Expression ◽

Prior Exposure ◽

Microrna Expression Profile

Gestational diabetes mellitus (GDM), one of the major pregnancy-related complications, characterized as a transitory form of diabetes induced by insulin resistance accompanied by a low/absent pancreatic beta-cell compensatory adaptation to the increased insulin demand, causes the acute, long-term, and transgenerational health complications. The aim of the study was to assess if alterations in gene expression of microRNAs associated with diabetes/cardiovascular/cerebrovascular diseases are present in whole peripheral blood of children aged 3–11 years descending from GDM complicated pregnancies. A substantially altered microRNA expression profile was found in children descending from GDM complicated pregnancies. Almost all microRNAs with the exception of miR-92a-3p, miR-155-5p, and miR-210-3p were upregulated. The microRNA expression profile also differed between children after normal and GDM complicated pregnancies in relation to the presence of overweight/obesity, prehypertension/hypertension, and/or valve problems and heart defects. Always, screening based on the combination of microRNAs was superior over using individual microRNAs, since at 10.0% false positive rate it was able to identify a large proportion of children with an aberrant microRNA expression profile (88.14% regardless of clinical findings, 75.41% with normal clinical findings, and 96.49% with abnormal clinical findings). In addition, the higher incidence of valve problems and heart defects was found in children with a prior exposure to GDM. The extensive file of predicted targets of all microRNAs aberrantly expressed in children descending from GDM complicated pregnancies indicates that a large group of these genes is involved in ontologies of diabetes/cardiovascular/cerebrovascular diseases. In general, children with a prior exposure to GDM are at higher risk of later development of diabetes mellitus and cardiovascular/cerebrovascular diseases, and would benefit from dispensarisation as well as implementation of primary prevention strategies.

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Polysaccharide IV from Lycium barbarum L. Improves Lipid Profiles of Gestational Diabetes Mellitus of Pregnancy by Upregulating ABCA1 and Downregulating Sterol Regulatory Element-Binding Transcription 1 via miR-33

Frontiers in Endocrinology ◽

10.3389/fendo.2018.00049 ◽

2018 ◽

Vol 9 ◽

Author(s):

Shuli Yang ◽

Lihui Si ◽

Limei Fan ◽

Wenwen Jian ◽

Huilin Pei ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Regulatory Element ◽

Lipid Profiles ◽

Lycium Barbarum ◽

Sterol Regulatory Element

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Effects of Risk Factors for Pregnancy Outcomes of Subsequent Pregnancy Associated with Gestational Diabetes Mellitus: A Retrospective Cohort Study in China.

10.21203/rs.3.rs-962585/v1 ◽

2021 ◽

Author(s):

Weijiao Xu ◽

Xia Xu ◽

Yanni Guo ◽

Jie Liang ◽

Jianying Yan

Keyword(s):

Diabetes Mellitus ◽

Risk Factors ◽

Blood Glucose ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Early Pregnancy ◽

Child Outcomes ◽

Fasting Blood Glucose ◽

Neonatal Outcomes ◽

Lipid Levels

Abstract BackgroundSince the implementation of the three‑child policy in China, the number of high-risk pregnant women has increased, causing serious challenges to health care during pregnancy. In this article, we aimed to investigate the impact of several risk factors for maternal and neonatal outcomes in pregnancies complicated by gestational diabetes mellitus (GDM) and recurrent GDM to formulate a management strategy to minimize the effect of risk factors for gestational diabetes. ResultsPre-pregnancy body mass index (BMI) and gestational weight gain affect maternal and child outcomes in the first and second onset of GDM. Pregnancy interval and fasting blood glucose in early pregnancy influence maternal and child outcomes of recurrent GDM. Maternal lipid levels during early pregnancy have a marked influence on neonatal outcomes in recurrent GDM.ConclusionsOn the basis of this result, weight management should be closely monitored before and during pregnancy. For planning of the second pregnancy with a previous history of GDM, a reasonable time between pregnancies is ideal. Moreover, in the next pregnancy, control of fasting blood glucose and lipid levels during the first trimester is necessary to improve both maternal and child outcomes.

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