The correlations between Th1 and Th2 cytokines in human alveolar echinococcosis

Abstract Background : Alveolar echinococcosis (AE) is a zoonotic parasitic disease caused by Echinococcus multilocularis larval tapeworm infections in humans that severely impairs the health of affected patients in the northern hemisphere. Methods : The expression levels of 20 cytokines associated with AE infection were measured by enzyme-linked immunosorbent assay, and the correlations between these cytokines were analysed in the R programming language. Results : Serum cytokine levels differed among individuals in both the AE patient and healthy control groups. The results of the correlations among the cytokines showed obvious differences between the two groups. In the AE patients group, Th1 and Th2 cytokines formed a more complicated network than that in the healthy control group. Conclusions : The altered correlations between Th1 and Th2 cytokines may be closely associated with AE infection, which may provide a new explanation for the essential differences between AE patients and healthy individuals.

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The correlations between Th1 and Th2 cytokines in human alveolar echinococcosis

10.21203/rs.2.21197/v1 ◽

2020 ◽

Author(s):

Xiao Ma ◽

Xuefei Zhang ◽

Jia Liu ◽

Yufang Liu ◽

Cunzhe Zhao ◽

...

Keyword(s):

Betweenness Centrality ◽

Alveolar Echinococcosis ◽

Healthy Control Group ◽

Control Group ◽

Th2 Cytokines ◽

High Betweenness ◽

Th1 And Th2 Cytokines ◽

Healthy Control ◽

High Betweenness Centrality ◽

Th1 And Th2

Abstract Background: Alveolar echinococcosis (AE) is a cosmopolitan zoonotic parasitic disease caused by Echinococcus multilocularis larval tapeworm infections in humans that severely impairs the health of affected patients. Methods: The expression levels of 20 cytokines associated with AE infection were measured by enzyme-linked immunosorbent assay, and the correlations between these cytokines were analysed in the R programming language. Results: Serum cytokine levels differed among individuals in both the AE patient and healthy control groups. Related studies have shown that different cytokines are associated with AE. Therefore, we investigated the correlations among the cytokines; these correlations were simple in the healthy control group butcomplex in the AE patient group. Th2 cytokines, such as interferon (IFN)-γ, interleukin (IL)-1β and monocyte chemoattractant protein (MCP)-1, had high betweenness centrality in AE patients, whereas Th1 cytokines, such as growth-regulated oncogene (GRO)-α, eotaxin and IL-5, had high betweenness centrality in the healthy control group. Conclusions: The altered correlations between Th1 and Th2 cytokines may be closely associated with AE progression.

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The expression and significance of inflammatory cytokines and MMP-9 in the tears of the infected eye and contralateral uninfected eye in patients with fungal keratitis

10.21203/rs.3.rs-269176/v1 ◽

2021 ◽

Author(s):

Shuang Zhang ◽

Hui-Min Wu ◽

Xiang-Ni Cao ◽

Xian-Qi Zhang ◽

Gui-ping Gao

Keyword(s):

Tear Film ◽

Fungal Keratitis ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Controls ◽

Tnf Α ◽

Cytokine Levels ◽

Healthy Control ◽

The Relationship ◽

Tear Film Function

Abstract Background: We investigated bilateral tear cytokine levels including interleukin (IL)-1β, IL-10, IL-17, tumor necrosis factor (TNF)-α and Matrix metalloproteinase-9 (MMP-9) in patients with fungal keratitis(FK). Meanwhile, we evaluated the relationship between the changes of tear cytokines with corneal perception and pain in infected eyes, and the relationship between tear cytokines and tear film function in contralateral uninfected eyes .Methods : A total of 60(20 FK, 20 contralateral, 20 healthy controls) tear samples were collected prospectively and analyzed by enzyme linked immunosorbent assay(ELISA). Approximately 50 to 60 ul of tear samples in each case were collected. Meanwhile ,we analyzed the changes of visual analogue scale(VAS), tear breakup time (TBUT), Schirmer I test (SIT) and corneal perception compared with healthy controls. Results :The concentrations of IL-1β, IL-10 and IL-17 increased in bilateral eyes compared with healthy controls(P<0.05). The tear concentrations of MMP-9 , TNF-α only significantly increased in affected eyes (P <0.05). Patients with FK showed significant reduction in corneal perception of infected eyes compared with controls(P<0.05). Corneal perception of the normal eyes in FK patients was slightly lower than that of control group, but there was not statistical difference (P>0.05).TBUT and SIT of contralateral uninfected eyes were significantly lower than that of control group(P<0.05), which were significantly correlated with levels of IL-1β, IL-17(P<0.05). SIT were also negatively correlated with MMP-9(P<0.05), while the levels of IL-1β, IL-10, IL-17, TNF-α and MMP-9 in the tears of the healthy control group had no significant correlation with TBUT and SIT indicators(P>0.05).The corneal perception and VAS score of the affected FK eyes showed correlation with IL-1β, IL-17 and TNF-α(P<0.05).In addition, concentration of IL-10 inversely was correlated with VAS (P<0.05). Conclusion: Proinflammatory tear cytokines are elevated in bilateral eyes with unilateral FK as associated with tear film function ,pain and corneal sensitivity.

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The correlations between Th1 and Th2 cytokines in human alveolar echinococcosis

BMC Infectious Diseases ◽

10.1186/s12879-020-05135-y ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Xiao Ma ◽

Xuefei Zhang ◽

Jia Liu ◽

Yufang Liu ◽

Cunzhe Zhao ◽

...

Keyword(s):

Alveolar Echinococcosis ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Th1 And Th2

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Th1 and Th2 cytokines in early onset periodontitis

Immunology Letters ◽

10.1016/s0165-2478(97)88489-1 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 407

Author(s):

Z Opatrná

Keyword(s):

Early Onset ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Th1 And Th2

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Effects of β-Lapachone on the Production of Th1 and Th2 Cytokines in C57BL/6 Mice

Journal of Environmental Pathology Toxicology and Oncology ◽

10.1615/jenvironpatholtoxicoloncol.v31.i2.10 ◽

2012 ◽

Vol 31 (2) ◽

pp. 87-94 ◽

Cited By ~ 1

Author(s):

Myungwon Choi ◽

Indal Park ◽

Jaehyun Ju ◽

Kun-Young Park ◽

Kwang Hyuk Kim

Keyword(s):

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Th1 And Th2

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Effects of Th1 and Th2 cytokines on cytokine production and ICAM-1 expression on synovial fibroblasts.

Annals of the Rheumatic Diseases ◽

10.1136/ard.54.7.560 ◽

1995 ◽

Vol 54 (7) ◽

pp. 560-565 ◽

Cited By ~ 22

Author(s):

J F Schlaak ◽

A Schwarting ◽

P Knolle ◽

K H Meyer zum Buschenfelde ◽

W Mayet

Keyword(s):

Cytokine Production ◽

Synovial Fibroblasts ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Th1 And Th2

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Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study

CNS Spectrums ◽

10.1017/s1092852917000803 ◽

2017 ◽

Vol 24 (03) ◽

pp. 333-337 ◽

Cited By ~ 5

Author(s):

Maiara Zeni-Graiff ◽

Adiel C. Rios ◽

Pawan K. Maurya ◽

Lucas B. Rizzo ◽

Sumit Sethi ◽

...

Keyword(s):

Oxidative Stress ◽

At Risk ◽

Superoxide Dismutase ◽

High Risk ◽

Glutathione Peroxidase ◽

Healthy Control Group ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Ultra High Risk ◽

Healthy Control

IntroductionOxidative stress has been documented in chronic schizophrenia and in the first episode of psychosis, but there are very little data on oxidative stress prior to the disease onset.ObjectiveThis work aimed to compare serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in young individuals at ultra-high risk (UHR) of developing psychosis with a comparison healthy control group (HC).MethodsThirteen UHR subjects and 29 age- and sex-matched healthy controls (HC) were enrolled in this study. Clinical assessment included the Comprehensive Assessment of At-Risk Mental States (CAARMS), the Semi-Structured Clinical Interview for DSM-IV Axis-I (SCID-I) or the Kiddie-SADS-Present and Lifetime Version (K-SADS-PL), and the Global Assessment of Functioning (GAF) scale. Activities of SOD and GPx were measured in serum by the spectrophotometric method using enzyme-linked immunosorbent assay kits.ResultsAfter adjusting for age and years of education, there was a significant lower activity of SOD and lower GPX activity in the UHR group compared to the healthy control group (rate ratio [RR]=0.330, 95% CI 0.187; 0.584, p<0.001 and RR=0.509, 95% CI 0.323; 0.803, p=0.004, respectively). There were also positive correlations between GAF functioning scores and GPx and SOD activities.ConclusionOur results suggest that oxidative imbalances could be present prior to the onset of full-blown psychosis, including in at-risk stages. Future studies should replicate and expand these results.

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The Th1 and Th2 cytokines IFN-γ and IL-4 antagonize the inhibition of monocyte IL-1 receptor antagonist by glucocorticoids: involvement of IL-1

European Journal of Immunology ◽

10.1002/(sici)1521-4141(199807)28:07<2075::aid-immu2075>3.0.co;2-0 ◽

1998 ◽

Vol 28 (7) ◽

pp. 2075-2085 ◽

Cited By ~ 14

Author(s):

Damián Kovalovsky ◽

Marcelo Páez Pereda ◽

Joachim Sauer ◽

Carolina Perez Castro ◽

Victor E. Nahmod ◽

...

Keyword(s):

Receptor Antagonist ◽

Th2 Cytokines ◽

Th1 And Th2 Cytokines ◽

Ifn Γ ◽

Th1 And Th2

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Urine CA-2 as a biomarker for diagnosis urinary stone and prediction of its complications

10.21203/rs.3.rs-59394/v1 ◽

2020 ◽

Author(s):

Yuan-jing Leng ◽

Hai-bin Zhou ◽

Jiang-ling Fu ◽

Wen-juan Wang

Keyword(s):

Healthy Subjects ◽

Characteristic Curve ◽

Urinary Stone ◽

Urinary Stones ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Control ◽

The Mean ◽

The Difference ◽

The Relationship

Abstract PURPOSECarbonic anhydrase-2 (CA-2) plays a role in mineralization and calcification in organism. Strong evidence suggests that CA-2 is associated with urolithiasis. However, the relationship between CA-2 and urinary stone remains unclear. The study aimed to assess the association of urine CA-2 (uCA-2) level and the potential risk of urinary stone.METHODSFrom March 2017 to November 2019, a prospective cohort study was conducted on patients with urinary stones and healthy subjects to determine the pretreatment uCA-2 level detection by Enzyme linked immunosorbent assay (ELISA). The difference of uCA-2 level between patients with urinary stones and healthy subjects was compared. Then comparison between stone patients with complications and without complications was carried out as well as correlation analysis to detect factors associated with biomarker expression.RESULTS118 patients with urinary stones were into urinary stones group and 42 healthy subjects were into healthy control group. The mean pretreatment uCA-2 level was significantly higher in patients with urinary stones group than healthy controls group (P=0.028). Furthermore, The uCA-2 level was positive correlation with urinary stones complications (R=0.379, P=0.000), especially pain complications (R=0.524, P=0.000) and hematuria complications (R=0.374, P=0.000). Receiver operating characteristic curve (ROC) analysis that a uCA-2 level threshold of 10.94 ng/mL had 83.67% sensitivity and 68.12% specificity for predicting urinary stones complications. CONCLUSIONSExcessive uCA-2 excretion is a major risk factor for urinary stone. Our findings suggested that uCA-2 may be used as an unappreciated biomarker for the diagnosis urinary stone in patients and to predict its complications.

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