scholarly journals Urine CA-2 as a biomarker for diagnosis urinary stone and prediction of its complications

2020 ◽  
Author(s):  
Yuan-jing Leng ◽  
Hai-bin Zhou ◽  
Jiang-ling Fu ◽  
Wen-juan Wang
Keyword(s):  
Healthy Subjects ◽  
Characteristic Curve ◽  
Urinary Stone ◽  
Urinary Stones ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Control ◽  
The Mean ◽  
The Difference ◽  
The Relationship

Abstract PURPOSECarbonic anhydrase-2 (CA-2) plays a role in mineralization and calcification in organism. Strong evidence suggests that CA-2 is associated with urolithiasis. However, the relationship between CA-2 and urinary stone remains unclear. The study aimed to assess the association of urine CA-2 (uCA-2) level and the potential risk of urinary stone.METHODSFrom March 2017 to November 2019, a prospective cohort study was conducted on patients with urinary stones and healthy subjects to determine the pretreatment uCA-2 level detection by Enzyme linked immunosorbent assay (ELISA). The difference of uCA-2 level between patients with urinary stones and healthy subjects was compared. Then comparison between stone patients with complications and without complications was carried out as well as correlation analysis to detect factors associated with biomarker expression.RESULTS118 patients with urinary stones were into urinary stones group and 42 healthy subjects were into healthy control group. The mean pretreatment uCA-2 level was significantly higher in patients with urinary stones group than healthy controls group (P=0.028). Furthermore, The uCA-2 level was positive correlation with urinary stones complications (R=0.379, P=0.000), especially pain complications (R=0.524, P=0.000) and hematuria complications (R=0.374, P=0.000). Receiver operating characteristic curve (ROC) analysis that a uCA-2 level threshold of 10.94 ng/mL had 83.67% sensitivity and 68.12% specificity for predicting urinary stones complications. CONCLUSIONSExcessive uCA-2 excretion is a major risk factor for urinary stone. Our findings suggested that uCA-2 may be used as an unappreciated biomarker for the diagnosis urinary stone in patients and to predict its complications.

scholarly journals The expression and significance of inflammatory cytokines and MMP-9 in the tears of the infected eye and contralateral uninfected eye in patients with fungal keratitis

2021 ◽  
Author(s):  
Shuang Zhang ◽  
Hui-Min Wu ◽  
Xiang-Ni Cao ◽  
Xian-Qi Zhang ◽  
Gui-ping Gao
Keyword(s):  
Tear Film ◽  
Fungal Keratitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Controls ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Healthy Control ◽  
The Relationship ◽  
Tear Film Function

Abstract Background: We investigated bilateral tear cytokine levels including interleukin (IL)-1β, IL-10, IL-17, tumor necrosis factor (TNF)-α and Matrix metalloproteinase-9 (MMP-9) in patients with fungal keratitis(FK). Meanwhile, we evaluated the relationship between the changes of tear cytokines with corneal perception and pain in infected eyes, and the relationship between tear cytokines and tear film function in contralateral uninfected eyes .Methods : A total of 60(20 FK, 20 contralateral, 20 healthy controls) tear samples were collected prospectively and analyzed by enzyme linked immunosorbent assay(ELISA). Approximately 50 to 60 ul of tear samples in each case were collected. Meanwhile ,we analyzed the changes of visual analogue scale(VAS), tear breakup time (TBUT), Schirmer I test (SIT) and corneal perception compared with healthy controls. Results :The concentrations of IL-1β, IL-10 and IL-17 increased in bilateral eyes compared with healthy controls(P<0.05). The tear concentrations of MMP-9 , TNF-α only significantly increased in affected eyes (P <0.05). Patients with FK showed significant reduction in corneal perception of infected eyes compared with controls(P<0.05). Corneal perception of the normal eyes in FK patients was slightly lower than that of control group, but there was not statistical difference (P>0.05).TBUT and SIT of contralateral uninfected eyes were significantly lower than that of control group(P<0.05), which were significantly correlated with levels of IL-1β, IL-17(P<0.05). SIT were also negatively correlated with MMP-9(P<0.05), while the levels of IL-1β, IL-10, IL-17, TNF-α and MMP-9 in the tears of the healthy control group had no significant correlation with TBUT and SIT indicators(P>0.05).The corneal perception and VAS score of the affected FK eyes showed correlation with IL-1β, IL-17 and TNF-α(P<0.05).In addition, concentration of IL-10 inversely was correlated with VAS (P<0.05). Conclusion: Proinflammatory tear cytokines are elevated in bilateral eyes with unilateral FK as associated with tear film function ,pain and corneal sensitivity.

Utility of EEG as Diagnostic and Tarapeutic Approach in Examiners in Forensic Population-prevention of Criminal Recidiv

2009 ◽  
Vol 24 (S1) ◽  
pp. 1-1
Author(s):  
V. Talevska
Keyword(s):  
Criminal Behavior ◽  
Control Group ◽  
Significant Relation ◽  
Forensic Population ◽  
Safety Measures ◽  
Significant Difference ◽  
Eeg Changes ◽  
The Mean ◽  
The Difference ◽  
The Relationship

Aims:To define the importance for the difference between the EEG in patients with criminal behavior and in control group. To examine the relationship between the EEG of the patients with criminal behavior and diagnosis as well as the prompt registration of EEG changes.Material and methods:158 EEG investigations examined at the Psychiatric Hospital in Demir Hisar for 24 months with aggressive and criminal behavior.Results and discussion:In men normal EEG (69.44%) prevails. The means age of patients in examiner group with abnormal EEG is significant higher from the means age in patient's with normal EEG. There was the significant difference between normal and abnormal EEG and the diagnoses. There is not significant difference between the patient's behavior and EEG, and there was significant difference between EEG and its criminal factor. There was not relation between EEG and the patient's sex in control group. The difference between the means age (37.73 god.) in control group with abnormal EEG and the mean age of patients with normal EEG is significant. There was not significant relation between EEG and patient's diagnosis of control group. There was not connection between patient's behavior and EEG in control group. There was significant difference between EEG in examiner group and in control group.Conclusion:There was significant difference between EEG in control and in examiner group.Suggested measures:Easily diagnosis providing of psychiatric disorders with aggressive and criminal behavior with EEG -help and safety measures at examiners with criminal behavior.

The Relationship Between the Imaging Characteristics of Tumor Angiogenesis and the Expression of Serum Cytokines in Lung Cancer

2020 ◽  
Vol 10 (9) ◽  
pp. 2228-2235
Author(s):  
Xihu Yao ◽  
Jianying Zhou ◽  
Zhicheng Zhang ◽  
Qingyun Zhou ◽  
Bernice Brown
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Blood Supply ◽  
Matrix Metalloproteinase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Lung Cancer Patients ◽  
Imaging Characteristics ◽  
The Difference ◽  
The Relationship

Objective: As for the lung cancer patients, their imaging characteristics of CT and DSA are evaluated, so as to analyze the relationship between the degree of angiogenesis and the expression of cytokines. Method: Firstly, the CT and DSA data of 40 lung cancer patients who are confirmed by pathology and received intravascular interventional therapy are collected. 5 mL venous blood of patients is extracted and serum samples are extracted. The levels of matrix metalloproteinase-2 (MMP-2) as well as basic fibroblast growth factor (bFGF) in 40 patients with lung cancer are detected by enzyme-linked immunosorbent assay (ELISA). Meanwhile, the contents of MMP-2 and bFGF in serum of 40 healthy people are collected as normal control group. Normal distribution, t-test and F-test are used to verify the correlation between the image features of lung cancer and the expression levels of MMP-2 and bFGF in serum. Results: Firstly, the levels of MMP-2 and bFGF in lung cancer group are obviously higher than those in the control group, and the difference was statistically significant. P values were 0.002 and 0, respectively (P < 0.01). In addition, a positive correlation exists between MMP-2 and bFGF in the group of lung cancer as well as control, and the correlation coefficients are 0.602 as well as 0.712, respectively. Secondly, DSA of lung cancer shows that MMP-2 and bFGF of different types of blood supply lung cancer are different. The P value of intra group and inter group comparison was 0, and the difference is statistically significant. Thirdly, on the image, the lung cancer has necrotic cavity. The P value of MMP-2 and bFGF are 0.046 and 0.005 respectively. The P value of MMP-2 and bFGF are 0.001 and 0.001 respectively with the burr sign on the image. The P value of serum MMP-2 and bFGF are 0 and 0.001 with the deep lobulated sign on the image, both of which are statistically significant. There is no correlation between the size and location of lung cancer focus, whether there is peripheral obstructive pneumonia and the level of MMP-2 and bFGF. Conclusion: The DSA blood supply of lung cancer is related to the expression of MMP-2 as well as bFGF in serum, which can reflect the angiogenic state of tumor and be used as the basis of anti-angiogenic therapy. Whether the CT image of lung cancer shows necrotic cavity, burr sign and deep lobulated sign has a correlation with the expression of MMP-2 as well as bFGF in the serum. The combination of the two is helpful for the accurate assessment of the disease.

scholarly journals Adropin and Irisin Deficiencies Are Associated With Presence of Diagonal Earlobe Crease in CAD Patients

2021 ◽  
Vol 8 ◽  
Author(s):  
Na Wei ◽  
Ronghuai Zhang ◽  
Zhanfang Zhu ◽  
Rui Li ◽  
Qi Yu ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Characteristic Curve ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Novel Biomarkers ◽  
The Difference ◽  
Artery Disease ◽  
Circulating Levels ◽  
Prognostic Power ◽  
Group Serum

Background and Aims: Diagonal earlobe crease (ELC) has been considered a potential cutaneous marker of atherosclerosis. However, the potential mechanism by which ELC and atherosclerosis are linked has not been adequately defined. Roles of adropin and irisin, novel biomarkers of endothelial function, in ELC have not been well-studied. This study aimed to test whether individuals with ELC are deficient in adropin and irisin, a characteristic that would likely promote endothelial dysfunction and provide a plausible common pathological basis for atherosclerosis and ELC.Methods: Patients diagnosed with coronary artery disease (CAD) with (n = 45) and without (n = 45) ELC were consecutively enrolled in the study. The ages of the patients enrolled ranged from 40–70 years. Other patients (n = 45) without ELC or CAD were recruited as the control group. All patients underwent coronary angiography. Serum adropin and irisin concentrations were assessed via enzyme-linked immunosorbent assay.Results: Circulating levels of irisin in the ELC group were significantly lower than those in the non-ELC group, and were highest in the control group. Serum adropin levels of the ELC group were significantly lower than those of the non-ELC group (P &lt; 0.001). Interestingly, although the serum adropin level of the control group was greater than that of the non-ELC group, the difference failed to achieve statistical significance. In subgroup analysis of CAD and ELC, both serum adropin and irisin levels of the CAD and ELC groups were lower than those of the control group (P &lt; 0.001). Receiver-operating characteristic curve analysis revealed that adropin and irisin have similar prognostic power for CAD and ELC.Conclusions: Low adropin and irisin were significantly associated with CAD and ELC. The deficiencies in adropin and irisin may be a common cause of both atherosclerosis and ELC, which explains why patients with ELC are prone to CAD.

SOMATOMEDIN PRODUCTION IN NORMAL ADULTS AND CIRRHOTIC PATIENTS

1977 ◽  
Vol 86 (2) ◽  
pp. 355-362 ◽  
Author(s):  
R. M. Schimpff ◽  
D. Lebrec ◽  
M. Donnadieu
Keyword(s):  
Blood Flow ◽  
Hepatic Blood Flow ◽  
Normal Subjects ◽  
Control Group ◽  
Cirrhotic Patients ◽  
The Mean ◽  
The Difference ◽  
The One ◽  
The Relationship ◽  
Normal Adults

ABSTRACT The somatomedin (SM) activity was measured in blood samples withdrawn simultaneously from hepatic (HV) and peripheral (PV) veins in 10 adult patients without liver disease (control group) and in 18 alcoholic patients with cirrhosis. SM activity in the control group was 1.27 ± 0.18 U/ml (mean ± sem) in the HV and 1.00 ± 0.17 U/ml in the PV. In patients with cirrhosis the SM activity was respectively 0.50 ± 0.05 and 0.56 ± 0.06 U/ml. SM activity was greater in the normal subjects than in the cirrhotic patients (P < 0.001 when SM activity measured in the HV, and P < 0.01 when measured in the PV). Close examination of the means of differences of SM activity between the HV and PV, showed that the differences were significantly different from 0 in control only (P < 0.02). At least, this difference (0.275 ± 0.093 U/ml) is greater (P < 0.01) than the one observed in cirrhotic patients (0.066 ± 0.036 U/ml). In 16 subjects, the hepatic blood flow was measured, and the mean hepatic SM activity production was estimated to be 398 ± 334 U/min in 6 subjects from the control group, and 16 U/min in 10 patients with cirrhosis. The difference is significant (P < 0.01). The relationship between SM activity, biochemical liver function parameters, hepatic blood flow and immunoreactive growth hormone were also studied. The results confirm that the liver is involved in serum somatomedin activity generation.

scholarly journals Circulating furin, IL-6, and presepsin levels and disease severity in SARS-CoV-2–infected patients

2021 ◽  
Vol 104 (2_suppl) ◽  
pp. 003685042110261
Author(s):  
Abdurrahim Kocyigit ◽  
Ozgur Sogut ◽  
Ezgi Durmus ◽  
Ebru Kanimdan ◽  
Eray Metin Guler ◽  
...  
Keyword(s):  
Disease Severity ◽  
Disease Activity Score ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytokine Release ◽  
Cytokine Release Syndrome ◽  
Vast Number ◽  
Angiotensin Converting Enzyme 2 ◽  
Healthy Control ◽  
The Mean ◽  
The Relationship

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a vast number of infections and deaths that deeply affect the world. When the virus encounters the host cell, it binds to angiotensin-converting enzyme 2, then the S protein of the virus is broken down by the transmembrane protease serine 2 with the help of furin, allowing the virus to enter the cell. The elevated inflammatory cytokines suggest that a cytokine storm, also known as cytokine release syndrome, may play a major role in the pathology of COVID-19. Therefore, the aim of this study is to investigate the relationship between circulating furin levels, disease severity, and inflammation in patients with SARS-CoV-2. A total of 52 SARS-CoV-2 patients and 36 healthy control participants were included in this study. SARS- CoV-2 patients were scored by the disease activity score. Serum furin, presepsin, and interleukin-6 (IL-6) levels were assessed using an enzyme-linked immunosorbent assay. The mean furin, presepsin, and IL-6 levels were significantly higher in the peripheral blood of SARS-CoV-2 compared to the controls ( p < 0.001). There were close positive relationship between serum furin and IL-6, furin and presepsin, and furin and disease severity ( r = 0.793, p < 0001; r = 0.521, p < 0.001; and r = 0,533, p < 0.001, respectively) in patients with SARS-CoV-2. These results suggest that furin may contribute to the exacerbation of SARS-CoV-2 infection and increased inflammation, and could be used as a predictor of disease severity in COVID-19 patients.

scholarly journals Passive Periodontal Sensation Threshold of Upper Molars in Patients with Myofascial Pain

2018 ◽  
Vol 12 (1) ◽  
pp. 1012-1020 ◽  
Author(s):  
Atsutoshi Hirata ◽  
Seiya Kato ◽  
Acing Habibie Mude ◽  
Kazuhiro Oki ◽  
Tadashi Matsunaga ◽  
...  
Keyword(s):  
Temporomandibular Disorders ◽  
Null Hypothesis ◽  
Myofascial Pain ◽  
Control Group ◽  
Significant Difference ◽  
Group A ◽  
The Mean ◽  
The Difference ◽  
Tooth Axis ◽  
The Relationship

Background and Objective: The relationship between periodontal sensation and Myofascial Pain (MP) is not yet fully clarified. The aim of this study was to test the null hypothesis that there is no difference in the periodontal sensation threshold between subjects with MP and subjects with no Temporomandibular Disorders (TMD). Methods: Participants have clinically assessed in accordance with the Research Diagnostic Criteria for Temporomandibular Disorders version 1.0 guidelines and assigned to the MP group (mean age 54.8 ± 14.8 years; 1 male and 11 females) or the control group (mean age: 63.9 ± 13.2 years; 1 male and 15 females). The Passive Periodontal Sensation Threshold (PPST) was evaluated using impulsive mechanical stimulation on the occlusal surface parallel to the tooth axis of the maxillary first molar, if present. The difference in the mean PPST between the MP group and the control group was evaluated using the Student t-test after checking for homoscedasticity. Results: The mean PPST value was 1050.1 ± 480.3 mN in the MP group and 712.3 ± 288.5 mN in the control group. A significant difference was observed between these mean PPST values (p = 0.045). Conclusion: There was a significant difference in PPST between the MP group and the control group. Although the etiology of the change of PPST is still unknown, the higher PPST value observed in MP patients suggests that future study on occlusal dysesthesia or occlusal sensation-related pathosis is warranted.

scholarly journals Role of Osteoprotegerin as Novel Marker in Urinary Stone Formers: A Pilot Study

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sreedhar Dayapule ◽  
SaiSwaroop Parthasarathy Yamajala ◽  
Ankit Anand ◽  
Ritvij Patankar ◽  
Bala Murali Krishna ◽  
...  
Keyword(s):  
Serum Levels ◽  
Urinary Stone ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Case Group ◽  
Metabolic Disturbances ◽  
Stone Formers ◽  
Calculus Formation ◽  
The Mean ◽  
The Difference

Background: Urinary tract calculus formation can be prevented by identifying molecules and metabolic disturbances that affect this process. Osteoprotegerin (OPG), a cytokine of the TNF receptor superfamily, has been demonstrated to mediate vascular calcification and intimal calcification. Endothelial injury and oxidative stress are known to play a role in urolithiasis in the form of Rendall’s plaques. Objectives: The present study aimed to compare 24-h urinary and serum OPG levels of patients with and without urolithiasis. Methods: In this case-control study, 24-h urinary levels of OPG (pg/mL), serum levels of OPG (pg/mL), and creatinine (mg/dL) were measured in both groups. Urinary and serum levels of OPG were determined by enzyme-linked immunosorbent assay (ELISA) using human OPG kits. Results: Mean serum creatinine was 0.86 ± 0.21 mg/dL in the case group and 0.77± 0.16 mg/dL in controls. The difference in the mean serum OPG levels between the cases (227.13 ± 98.02 pg/mL) and controls (47.28 ± 29.61 pg/mL) was highly significant (P value < 0.0001). The difference in the mean 24-h urinary OPG levels between the cases (156.12 ± 174.31 pg/mL) and controls (9.32 ± 23.72 pg/mL) was highly significant (P value < 0.001). Conclusions: There were significantly higher levels of OPG in serum and 24-h urine samples of cases than in controls. Hence, it requires further large studies to make OPG a diagnostic and prognostic marker.

Vitamin D Treatment in Patients with Hashimoto’s Thyroiditis may Decrease the Development of Hypothyroidism

2016 ◽  
Vol 86 (1-2) ◽  
pp. 9-17 ◽  
Author(s):  
Bekir Ucan ◽  
Mustafa Sahin ◽  
Muyesser Sayki Arslan ◽  
Nujen Colak Bozkurt ◽  
Muhammed Kizilgul ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Hashimoto’S Thyroiditis ◽  
Healthy Control Group ◽  
Hashimoto's Thyroiditis ◽  
Control Group ◽  
Endocrine Society ◽  
Thyroid Autoantibody ◽  
Healthy Control ◽  
The Mean

Abstract.The relationship between Hashimoto’s thyroiditis and vitamin D has been demonstrated in several studies. The aim of the present study was to evaluate vitamin D concentrations in patients with Hashimoto’s thyroiditis, the effect of vitamin D therapy on the course of disease, and to determine changes in thyroid autoantibody status and cardiovascular risk after vitamin D therapy. We included 75 patients with Hashimoto’s thyroiditis and 43 healthy individuals. Vitamin D deficiency is defined as a 25-hydroxy vitamin D (25(OH)D3) concentration less than 20ng/mL. Vitamin D deficient patients were given 50.000 units of 25(OH)D3 weekly for eight weeks in accordance with the Endocrine Society guidelines. All evaluations were repeated after 2 months of treatment. Patients with Hashimoto’s thyroiditis had significantly lower vitamin D concentrations compared with the controls (9.37±0.69 ng/mL vs 11.95±1.01 ng/mL, p < 0.05, respectively). Thyroid autoantibodies were significantly decreased by vitamin D replacement treatment in patients with euthyroid Hashimoto’s thyroiditis. Also, HDL cholesterol concentrations improved in the euthyroid Hashimoto group after treatment. The mean free thyroxine (fT4) concentrations were 0.89±0.02 ng/dL in patients with Hashimoto’s thyroiditis and 1.07±0.03 ng/dL in the healthy control group (p < 0.001). The mean thyroid volumes were 7.71±0.44 mL in patients with Hashimoto’s thyroiditis and 5.46±0.63 mL in the healthy control group (p < 0.01). Vitamin D deficiency is frequent in Hashimoto’s thyroiditis and treatment of patients with this condition with Vitamin D may slow down the course of development of hypothyroidism and also decrease cardiovascular risks in these patients. Vitamin D measurement and replacement may be critical in these patients.

Association of Serum Homocysteine Level and Interstitial Lung Disease in Systemic Sclerosis: A Case-control Study

2018 ◽  
Vol 15 (1) ◽  
pp. 74-78
Author(s):  
Mohammadali Nazarinia ◽  
Asghar Zare ◽  
Mohammad javad Fallahi ◽  
Mesbah Shams
Keyword(s):  
Systemic Sclerosis ◽  
Healthy Subjects ◽  
Case Control Study ◽  
Homocysteine Level ◽  
Case Control ◽  
Control Group ◽  
Lung Involvement ◽  
Serum Homocysteine ◽  
The Mean ◽  
Control Study

Background:Systemic sclerosis is a disorder of connective tissue with unknown cause, affecting the skin and internal organs, characterized by fibrotic changes.Objective:To determine the correlation between serum homocysteine level and interstitial lung involvement in systemic sclerosis. </P><P> Materials and Methods: In this case – control study, 59 patients who fulfilled the ACR/EULAR classification criteria for systemic sclerosis and were referred to Hafez Hospital of Shiraz, Iran, were included as the case group. Fifty nine healthy subjects were involved as the control group. Patients were divided into two groups based on interstitial lung involvement and two subtypes, diffuse and limited type. Serum homocysteine, vitamin B12, and folate levels compared between the controls, and cases groups.Results:Of 59 case and control group, 53 (%89.8) were female and the mean age did not differ in both groups (P=0.929). Thirty five (%59.3) patients had interstitial lung involvement and 38(%64.4) had diffuse cutaneous systemic sclerosis. The mean serum homocysteine level was 13.9±6.3 µmol/L in the case and 13.7±9.2 µmol/L in the control group (P=0.86). The mean serum homocysteine level did not differ between the patients with and without interstitial lung involvement (P=0.52). The patients with lung involvement was older than those without lung involvement (P=0.004). Lung disease was more common in diffuse type (P=0.014).Conclusion:In our study, serum homocysteine level did not differ between the patients and healthy subjects. Also, there was no correlation between serum homocysteine level and lung involvement, but lung involvement was more common in older patients and also diffuse subtype.

Sign in / Sign up

Export Citation Format

Share Document