Resistance profile from Staphylococcus aureus and Pseudomonas aeruginosa Obtained from tracheostomized children

Abstract Background The tracheostomized patients exhibit high risks of bacterial infections, because the tracheal tube acts as a gateway to these microorganisms. The objective was to characterize microbiologically the tracheal secretion of tracheostomized children, to evaluate the biofilm formation, and to study the phenotypic and molecular profile of antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated. Methods The study collected 88 tracheal secretion samples. The material processed by phenotypic tests were performed for bacterial identification. For identification of the biofilm, the Congo red agar test and the plaque microtiter test were used, and the qPCR method was used to resistance verification. Results Were obtained 27 samples of S. aureus and 71 of P. aeruginosa . All S. aureus samples were positive for biofilm formation on Congo red agar test. In antibiogram test, S. aureus showed resistance to seven drugs. Regarding the identification of resistance genes, were amplificated bla Z in 42.8% from S. aureus and mec A in 28.6% of them. Pseudomonas aeruginosa presented resistance to eight drugs. The most frequent chromosomal genes were bla OXA with 66.7% and bla KPC with 58.3%. To plasmidial DNA, was highlighted bla NDM with 58.3% positive. ConclusionThe S. aureus and P. aeruginosa characterization of colonization from lower respiratory tract associated with the use of the device in tracheotomy patients, and the physiology and antimicrobial resistance profile, will help health professionals to choose the most appropriate treatment to be administered in children with tracheotomy, increasing the chances of airway restoration and decannulation.

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Resistance Profile From Staphylococcus Aureus And Pseudomonas Aeruginosa Obtained From Tracheostomized Children

10.21203/rs.2.23521/v2 ◽

2020 ◽

Author(s):

Juliana Afonso de Almeida ◽

Caroline Espíndola de Barros ◽

Gustavo Henrique da Silva Ayres ◽

Mariana Helena e Silva ◽

Andressa Santos Liberal ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Resistance ◽

Congo Red ◽

Biofilm Formation ◽

Bacterial Infections ◽

Molecular Profile ◽

Resistance Profile ◽

Phenotypic Tests ◽

Tracheal Secretion

Abstract Background The tracheostomized patients exhibit high risks of bacterial infections, because the tracheal tube acts as a gateway to these microorganisms. The objective was to characterize microbiologically the tracheal secretion of tracheostomized children, to evaluate the biofilm formation, and to study the phenotypic and molecular profile of antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated. Methods The study collected 88 tracheal secretion samples. The material processed by phenotypic tests were performed for bacterial identification. For identification of the biofilm, the Congo red agar test and the plaque microtiter test were used, and the qPCR method was used to resistance verification. Results Were obtained 27 samples of S. aureus and 71 of P. aeruginosa . All S. aureus samples were positive for biofilm formation on Congo red agar test. In antibiogram test, S. aureus showed resistance to seven drugs. Regarding the identification of resistance genes, were amplificated bla Z in 42.8% from S. aureus and mec A in 28.6% of them. Pseudomonas aeruginosa presented resistance to eight drugs. The most frequent chromosomal genes were bla OXA with 66.7% and bla KPC with 58.3%. To plasmidial DNA, was highlighted bla NDM with 58.3% positive. Conclusion The S. aureus and P. aeruginosa characterization of colonization from lower respiratory tract associated with the use of the device in tracheotomy patients, and the physiology and antimicrobial resistance profile, will help health professionals to choose the most appropriate treatment to be administered in children with tracheotomy, increasing the chances of airway restoration and decannulation.

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Antimicrobial Resistance and Biofilm Formation of Pseudomonas aeruginosa

The International Arabic Journal of Antimicrobial Agents ◽

10.3823/846 ◽

2020 ◽

Vol 10 (2) ◽

Author(s):

Abdelraouf A Elmanama ◽

Suhaila Al-Sheboul ◽

Renad I Abu-Dan

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Quorum Sensing ◽

Bacterial Adhesion ◽

Biofilm Formation ◽

Bacterial Infections ◽

Developmental Stages ◽

Infection Site ◽

Major Virulence Factor

Abstract Pseudomonas aeruginosa threatens patient’s care. It is considered as the most complicated health care associated pathogen to be eliminated from infection site. The biofilm forming ability of P. aeruginosa, being a major virulence factor for most pathogenic microorganism, protects it from host immunity and contribute to antibiotic resistance of this organism. It is estimated that about 80% of infectious diseases are due to biofilm mode of growth. Biofilm forming ability of bacteria imparts antimicrobial resistance that leads to many persistent and chronic bacterial infections. The world is becoming increasingly under the threat of entering the “post-antibiotic era”, an era in which the rate of death from bacterial infections is higher than from cancer. This review focus on P. aeruginosa biofilm forming ability; definition, developmental stages, and significance. In addition, the quorum sensing and the antibiotic resistance of this pathogen is discussed. Keywords: Biofilm; bacterial adhesion; Pseudomonas aeruginosa; antimicrobial resistance; quorum sensing.

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Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms by Pseudomonas aeruginosa and Staphylococcus aureus

Applied and Environmental Microbiology ◽

10.1128/aem.02683-08 ◽

2009 ◽

Vol 75 (11) ◽

pp. 3586-3592 ◽

Cited By ~ 65

Author(s):

Phat L. Tran ◽

Adrienne A. Hammond ◽

Thomas Mosley ◽

Janette Cortez ◽

Tracy Gray ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

Bacterial Attachment ◽

Wound Dressings ◽

Aqueous Environment ◽

Burn Victims ◽

Surgical Wounds

ABSTRACT Among the most difficult bacterial infections encountered in treating patients are wound infections, which may occur in burn victims, patients with traumatic wounds, necrotic lesions in people with diabetes, and patients with surgical wounds. Within a wound, infecting bacteria frequently develop biofilms. Many current wound dressings are impregnated with antimicrobial agents, such as silver or antibiotics. Diffusion of the agent(s) from the dressing may damage or destroy nearby healthy tissue as well as compromise the effectiveness of the dressing. In contrast, the antimicrobial agent selenium can be covalently attached to the surfaces of a dressing, prolonging its effectiveness. We examined the effectiveness of an organoselenium coating on cellulose discs in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus biofilm formation. Colony biofilm assays revealed that cellulose discs coated with organoselenium completely inhibited P. aeruginosa and S. aureus biofilm formation. Scanning electron microscopy of the cellulose discs confirmed these results. Additionally, the coating on the cellulose discs was stable and effective after a week of incubation in phosphate-buffered saline. These results demonstrate that 0.2% selenium in a coating on cellulose discs effectively inhibits bacterial attachment and biofilm formation and that, unlike other antimicrobial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating.

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Antimicrobial resistance profile of microbial pathogens isolated from hospitals of Colatina-ES in the period of 2008-2010

Revista de Investigação Biomédica ◽

10.24863/rib.v8i1.30 ◽

2016 ◽

Vol 8 (1) ◽

pp. 61

Author(s):

Deuvânia Cabral Azevedo ◽

Marcus Vinícius Dias-Souza ◽

Andrea De Souza Monteiro

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Resistance ◽

Microbial Pathogens ◽

Resistance Profile ◽

E Coli ◽

Antimicrobial Resistance Profile ◽

Santa Maria

A resistência bacteriana é um mecanismo usado pelas bactérias para interferir na ação dos antibióticos e vem crescendo consideravelmente, se tornando um grande problema de saúde pública. O presente estudo avaliou o perfil de resistência de cepas de Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli e a suscetibilidade das mesmas à alguns antimicrobianos. Para isso realizou-se uma pesquisa quantitativa utilizando os resultados de antibiogramas constantes nos livros de registro microbiologia do laboratório LAC - Santa Maria, situado em Colatina - ES. As amostras foram coletadas rotineiramente de pacientes internados em dois hospitais (Santa Maria e São José) e examinados pelo laboratório. No livro de registro obteve-se um total de 446 isolados microbianos identificados, dos quais 277 cepas foram identificadas como: S. aureus (36,5%), P. aeruginosa (32,5%) e E. coli (31%), os 169 restantes englobam os outros micro-organismos. Quanto ao perfil de resistência e sensibilidade, as cepas de P. aeruginosa foram mais sensíveis ao antibiótico sulbactam, E. Coli à ampicilina e ácido nalidíxico, e Staphylococcus à amicacina e ceftriaxone. Relaciona-se o maior isolamento de Stapylococcus, P. aeruginosa e E. coli provavelmente por serem micro-organismos encontrados colonizando ambientes hospitalares e a comunidade com grande potencial de disseminação. Quanto ao perfil de resistência se torna útil na seleção de drogas especificas para determinados micro-organismo. Palavras-chave: Resistência. Antibióticos. Micro-organismos.

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Biofilm Formation by Staphylococcus Aureus and Pseudomonas Aeruginosa is Associated with an Unfavorable Evolution after Surgery for Chronic Sinusitis and Nasal Polyposis

Otolaryngology ◽

10.1016/j.otohns.2006.03.001 ◽

2006 ◽

Vol 134 (6) ◽

pp. 991-996 ◽

Cited By ~ 179

Author(s):

Zohra Bendouah ◽

Jean Barbeau ◽

Walid Abou Hamad ◽

Martin Desrosiers

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Nasal Polyposis ◽

Chronic Sinusitis

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Pseudomonas aeruginosa and Staphylococcus aureus virulence factors as biomarkers of infection

Biological Chemistry ◽

10.1515/hsz-2021-0243 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Artur J. Sabat ◽

Daniele Pantano ◽

Viktoria Akkerboom ◽

Erik Bathoorn ◽

Alexander W. Friedrich

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacterial Infections ◽

Clinical Status ◽

Clinical Samples ◽

Pathogenic Microorganisms ◽

Bacterial Identification ◽

Accurate Identification ◽

Culture Independent ◽

And Staphylococcus Aureus

Abstract The gold standard for the diagnosis of bacterial infections in clinical samples is based on culture tests that are time-consuming and labor-intense. For these reasons, an extraordinary effort has been made to identify biomarkers as the tools for sensitive, rapid and accurate identification of pathogenic microorganisms. Moreover, biomarkers have been tested to distinguish colonization from infection, monitor disease progression, determine the clinical status of patients or predict clinical outcomes. This mini-review describes Pseudomonas aeruginosa and Staphylococcus aureus biomarkers, which contribute to pathogenesis and have been used in culture-independent bacterial identification directly from patient samples.

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BIOFILM FORMATION OF STAPHYLOCOCCUS AUREUS, SALMONELLA ENTERITIDIS AND PSEUDOMONAS AERUGINOSA IN PRESENCE OF N-BENZOTIAZOL-2-YL-BENZENSULFONAMIDE DERIVATIVES

Microbiology&Biotechnology ◽

10.18524/2307-4663.2010.3(11).99012 ◽

2010 ◽

Vol 0 (3(11)) ◽

pp. 32-40

Author(s):

I. О. Малярчик ◽

Т. О. Філіпова ◽

Б. М. Галкін ◽

Б. М. Галкін

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Salmonella Enteritidis

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Isolation, Characterization, and Comparison of Efficiencies of Bacteriophages to Reduce Planktonic and Biofilm-Associated Staphylococcus aureus

Journal of Health and Allied Sciences NU ◽

10.1055/s-0040-1715773 ◽

2020 ◽

Vol 10 (03) ◽

pp. 102-108

Author(s):

Anoopkrishna Rai ◽

Rajeshwari V. Vittal ◽

Juliet R. Mohan Raj

Keyword(s):

Staphylococcus Aureus ◽

Biofilm Formation ◽

Bacterial Infections ◽

Viable Cell ◽

Viable Count ◽

Dna Fingerprint ◽

Planktonic Cells ◽

Microtiter Plates ◽

Reduction Assay ◽

Need To Evaluate

Abstract Introduction In the present era, wherein occurrence of antimicrobial resistance compounded with biofilms in disease conditions has rendered present antibiotic therapy ineffective, the need for alternative strategies to treat bacterial infections has brought bacteriophages to the forefront. The antimicrobial activity of phages is often determined by a viable cell reduction assay which focuses only on planktonic forms. The physiology of an organism in biofilm differs from those that are planktonic; hence, there is a need to evaluate the activity of phages both on planktonic forms, as well as on biofilms, to select candidate therapeutic phages. Materials and Methods Bacteriophages for Staphylococcus aureus were isolated from environmental samples and characterized based on growth kinetics and DNA fingerprint patterns. Activity of isolated phages on planktonic forms was determined by viable count reduction assay. Phage ability to prevent biofilm formation and ability to disperse formed biofilms were performed in 96-well microtiter plates and biofilm estimated by crystal violet assay. Results Four bacteriophages designated, that is, P3, PD1, PE1, and PE2, were isolated and characterized. Planktonic cells of S. aureus were found to be sensitive to phages PD1, PE1, and PE2. Phages PD1 and PE2 were efficient in preventing biofilm formation and phages PD1, PE1, and P3 were efficient in dispersing formed biofilms. Conclusion The ability of some phages to disperse biofilms effectively, while unable to show the same efficiency on planktonic cells, indicates that viable count reduction assay alone may not be a sufficient tool to imply bactericidal activity of bacteriophages, especially while trying to eradicate biofilms.

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