scholarly journals Muscle-Derived Stem Cells Combined With Nerve Growth Factor Transplantation in the Treatment of Stress Urinary Incontinence in Rats

2020 ◽  
Author(s):  
Jianxiong Fang ◽  
Haosheng Liu ◽  
Tianqi Liu ◽  
Zhenhui Zhang ◽  
Chao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Urinary Incontinence ◽  
Stem Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Stress Urinary Incontinence ◽  
Western Blot ◽  
Control Group ◽  
Nerve Growth

Abstract Objective: To investigate the efficacy of muscle-derived stem cells (MDSCs) combined with nerve growth factor (NGF) in the treatment of stress urinary incontinence (SUI) in rats. Methods: MDSCs were isolated and extracted from 90 SD rats, and the stem cell characteristics of the cells were identified using flow cytometry. NGF overexpression plasmid was coated with adenovirus and qRT-PCR was applied to verify adenovirus transfection efficiency. The rat models of SUI were constructed and randomly divided into five groups: control group, PBS group, MDSCs+oe-NGF group, MDSCs+vector group, and MDSCs group. After 8 weeks of feeding, the effect of MDSCs combined with NGF on repairing SUI in rats was detected by measuring the leakage point pressure (LPP) rats and Masson staining of rat urethral sections. The expression of NGF and VEGF was detected by western blot and IHC staining. Results: Flow cytometry results showed that the isolated MDSCs had stem cell characteristics and good purity. NGF mRNA was significantly upregulated in MDSCs after transfection with adenovirus coated with NGF plasmid. Compared with the control group, the LPP and the ratio of muscle fibers/collagen fibers were significantly increased in the MDSCs treated groups, with the highest increase in the MDSCs + oe-NGF group. Western blot and IHC results showed that compared with the control group, the expression of NGF and VEGF in the urethral tissues in the MDSCs treated groups were significantly up-regulated, with the highest increase in the MDSCs + oe-NGF group. Conclusion: MDSCs alone can improve SUI in rats, while MDSCs combined with NGF is more effective in the treatment of SUI rats by up-regulating VEGF.

scholarly journals Ultrasound Combined With Microbubbles Loading BDNF Retrovirus to Open Blood–Brain Barrier for Treatment of Alzheimer’s Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Feng Wang ◽  
Xi-Xi Wei ◽  
Lian-Sheng Chang ◽  
Lei Dong ◽  
Yong-Ling Wang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Western Blot ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Control Group ◽  
Nerve Growth ◽  
Blood Brain

Background: Brain-derived nerve growth factor (BDNF) is a promising effective target for the treatment of Alzheimer’s disease (AD). BDNF, which has a high molecular weight, has difficulty in crossing the blood–brain barrier (BBB). The study aimed to prepare microbubbles loading brain-derived nerve growth factor (BDNF) retrovirus (MpLXSN-BDNF), to verify the characteristics of the microbubbles, and to study the therapeutic effect of the microbubbles combined with ultrasound on the opening of the blood–brain barrier in an AD rat model.Methods: 32 adult male SD rats were randomly divided into four groups: control group, ultrasound + pLXSN-EGFP microbubble group (U + MpLXSN-BDNF), ultrasound + pLXSN-BDNF microbubble group, and ultrasound + microbubble + pLXSN-BDNF virus group (U + MpLXSN-BDNF), with eight rats in each group. At the same time, the left hippocampus of rats was irradiated with low-frequency focused ultrasound guided by MRI to open the blood–brain barrier (BBB). The effects of BDNF overexpression on AD rats were evaluated behaviorally before and 1 month after the treatment. The number of acetylcholinesterase (ChAT)-positive cells and the content of acetylcholine (ACh) in brain tissues were determined by immunohistochemistry and high-performance liquid chromatography (HPLC), respectively. IF staining of synaptic spines and Western blot of synaptophysin presented herein detected synaptic density recovery.Results: Signal intensity enhancement at the BBB disruption sites could be observed on the MR images. The behavioral evaluation showed that the times of crossing the original platform in the U + MpLXSN-BDNF group increased significantly after treatment. Immunohistochemistry and HPLC revealed that the number of ChAT-positive neurons and the contents of ACh in the brain were significantly decreased in the treated groups compared with the controls. IF staining of synaptic spines and Western blot data of synaptophysin showed that the U + MpLXSN-BDNF group can recover the synaptic loss better by BDNF supplementation than the other treatment groups.Conclusion: Ultrasound combined with viral microbubbles carrying BDNF can increase the transfection efficiency of brain neurons, promote the high expression of exogenous gene BDNF, and play a therapeutic role in the AD model rats.

scholarly journals Age-Related Yield of Adipose-Derived Stem Cells Bearing the Low-Affinity Nerve Growth Factor Receptor

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Raquel Cuevas-Diaz Duran ◽  
Maria Teresa González-Garza ◽  
Alejandro Cardenas-Lopez ◽  
Luis Chavez-Castilla ◽  
Delia Elva Cruz-Vega ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Nerve Growth Factor Receptor ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
Nerve Growth ◽  
Age Related

Adipose-derived stem cells (ADSCs) are a heterogeneous cell population that may be enriched by positive selection with antibodies against the low-affinity nerve growth factor receptor (LNGFR or CD271), yielding a selective cell universe with higher proliferation and differentiation potential. This paper addresses the need for determining the quantity of ADSCs positive for the CD271 receptor and its correlation with donor's age. Mononuclear cells were harvested from the lower backs of 35 female donors and purified using magnetic beads. Multipotency capacity was tested by the expression of stemness genes and through differentiation into preosteoblasts and adipocytes. A significant statistical difference was found in CD271+concentrations between defined age intervals. The highest yield was found within women on the 30–40-year-old age range. CD271+ADSCs from all age groups showed differentiation capabilities as well as expression of typical multipotent stem cell genes. Our data suggest that the amount of CD271+cells correlates inversely with age. However, the ability to obtain these cells was maintained through all age ranges with a yield higher than what has been reported from bone marrow. Our findings propose CD271+ADSCs as the primary choice for tissue regeneration and autologous stem cell therapies in older subjects.

Mesenchymal Stem Cells Enhanced Cardiac Nerve Sprouting via Nerve Growth Factor in a Rat Model of Myocardial Infarction

2014 ◽  
Vol 20 (12) ◽  
pp. 2023-2029 ◽  
Author(s):  
Jian Chen ◽  
Shaoxin Zheng ◽  
Hui Huang ◽  
Suihua Huang ◽  
Changqing Zhou ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Model ◽  
Nerve Growth ◽  
Cardiac Nerve ◽  
Nerve Sprouting
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