scholarly journals Development of Brassica Napus L. Ogu-INRA CMS Restorers Using Recurrent Full-Sib Selection.

Author(s):  
Valeria Lobos-Sujo ◽  
Robert W. Duncan

Abstract The Ogu-INRA CMS system in canola and rapeseed (Brassica napus L.) uses a cytological variant of the radish- (Raphanus sativus L.) derived Ogu CMS pollination control system introduced through interspecific introgression. The restorers (R-lines) contain an introgression that is associated with poor agronomic performance due to a large undesired segment of the radish chromosome that was introgressed along with the Rfo gene. The introgression contains pentatricopeptide (PPR) motif repeats that confer fertility restoration abilities to the R-lines. The objective of this research was to test the hypothesis that multiple cycles of intermating will result in R-lines with improved agronomic performance. A base population was developed by designing five R-line by R-line crosses. Twelve plants from each initial cross were grown and chain-crossed at random, without selection, other than the presence of the Rfo gene. Twelve flowers from each plant were crossed and the remainder of the plant was selfed. Three intermating crossing cycles (C0, C1 and C2) were completed and each was selfed three times for evaluation. Total pod number, seeds per pod, a visual pod rating, thousand seed weight and yield were evaluated. The visual pod rating showed a positive correlation with seeds per pod. Improvements for all traits were found at C0 and C1 when compared to the best parent. Individual families from two of the crosses showed a yield increase of over 78 % from the best parent. This suggests that improvements in yield components can be obtained from intermating R-lines.

1987 ◽  
Vol 67 (3) ◽  
pp. 545-559 ◽  
Author(s):  
W. F. NUTTALL ◽  
H. UKRAINETZ ◽  
J. W. B. STEWART ◽  
D. T. SPURR

In northeastern Saskatchewan on Gray Luvisolic soils, rapeseed (Brassica napus L. and B. campestris L.) grown on many fields does not set seed, possibly because of deficiencies of S and B. Therefore, experiments were begun in 1979 to determine (1) the effect of N, S and B fertilizers on yield and quality of rapeseed; (2) if cultivars (B. napus and B. campestris L.) responded differently to these nutrients; and (3) nutrient and nutrient interaction effects of five rates of N, S and B in a composite rotatable design on yield and quality of the cultivar Regent (B. napus L.). Rates of up to 200 kg N ha−1, 50 kg S ha−1 and 2.8 kg B ha−1 were applied. The experiments were conducted on 13 sites. Nine were in N.E. Saskatchewan on Sylvania f1, Waitville 1 (Luvisolic) and Melfort sicl (Black Chernozemic) soils. Four were in N.W. Saskatchewan on Loon River 1 and Waitville 1, (Luvisolic) soils. In N.W. Saskatchewan there was a significant yield increase because of N (1.00 t ha−1) and S (1.06 t ha−1). In N.E. Saskatchewan on Sylvania f1, rapeseed yields were increased by 0.38 t ha−1 by a combination of S and B and by 0.78 t ha−1 by N. Sylvania f1 soils were lower in soluble B than other experimental sites. At other sites in N.E. Saskatchewan, N but not S increased rapeseed grain yield significantly. Significant response to a combination of S and B was obtained with the cultivar Regent, and both species of rapeseed responded to S fertilizer. Sulphur fertilizer increased the glucosinolate concentration in rapeseed meal at all sites. Sulphur increased oil concentration of rapeseed on all sites except one where frost damaged the crop and increased protein of grain on sites where there was yield response to S. Nitrogen increased protein of rapeseed grown on all sites whereas N combined with B decreased protein and increased oil percentage on all sites except Sylvania f1. The yield response of the cultivar Regent to B was not significantly related to soluble soil B. The combined yield response to S and B in relation to soluble soil S and B was significant (R2 = 0.60). Yield response of rapeseed to S was significantly related to soluble soil S (R2 = 0.35). In conclusion, S fertilizer solved the problem of poor seed set in rapeseed cultivars, but B also enhanced yield by decreasing the number of sterile florets and improving pod development. Key words: Nitrogen, sulphur, boron, rapeseed, oil, protein, glucosinolates


2016 ◽  
Vol 41 (3) ◽  
pp. 529-540
Author(s):  
MA Miah ◽  
MG Rasul ◽  
MAK Mian

Identification of male fertility restorer genotypes for rapeseed CMS lines towards hybrid development in spring habit rapeseed (Brassica napus L.) adapted for short day winter season was studied. The experiment was conducted at the experimental farm and laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur during October, 2008 to March, 2011. An exotic CMS-based F1 hybrid of rapeseed was selfed to get F2 generation with a view to resynthesizing restorer line. As a result a restorer line for Nap248A Z1 and Nap248A Z2 cytoplasmic male sterile lines was identified in the F3 generation of the exotic F1 rapeseed hybrid which appears as the first case so far reported as achievement in Bangladesh in this regard. Genetic analysis further revealed fertility restoration for Nap248A Z1 and Nap248A Z2 cytoplasmic male sterility was controlled by a single dominant nuclear gene as a simple genetic phenomenon.Bangladesh J. Agril. Res. 41(3): 529-540, September 2016


2021 ◽  
Vol 19 (1) ◽  
pp. 15-25
Author(s):  
S Sultana ◽  
F Mahmud ◽  
M Asaduzzaman

Rapeseed (Brassica napus L.) is the best one in respect of oil production. It is the order of the day to take better steps for production and quality improvement of our local cultivars. Broadening of genetic diversity in spring oilseed Brassica napus L. (AACC, 2n = 38), canola is important for continued improvement of this crop. Sixty-two genotypes in F4 generation of Brassica napus L. were evaluated to assess genetic diversity based on randomized complete block design with three replications at the experimental field of Sher-e-Bangla Agricultural University, Dhaka. Different Multivariate analyses were performed to classify 62 genotypes. On the basis of cluster analysis, all the genotypes were classified in five clusters. The cluster IV comprised the maximum number (19) of genotypes followed by same in cluster II (18). The cluster I and V comprised 10 and 9 genotypes respectively. The lowest number of genotypes was present in cluster II. The highest inter-cluster distance (10.309) was observed between the cluster I and IV and the genotypes of these clusters involved in hybridization may produce a wide spectrum of segregating population. The lowest inter-cluster distance (3.513) was observed between the cluster III and IV. The inter-cluster distances were larger than the intra-cluster distances. Considering cluster distance, inter genotypic distance and other agronomic performance G3, G4, G24, G35 and G51 might be suggested for future breeding program. SAARC J. Agric., 19(1): 15-25 (2021)


1987 ◽  
Vol 67 (2) ◽  
pp. 491-493 ◽  
Author(s):  
A. J. KLASSEN ◽  
R. K. DOWNEY ◽  
J. J. CAPCARA

Westar summer rape (Brassica napus L.), licensed in 1982, is a canola-quality cultivar that combines high seed yield with high oil content and early maturity. Westar is adapted to the B. napus growing areas of western Canada and because of its superior agronomic performance has replaced the older cultivars Regent, Andor and Altex.Key words: Rape (summer), cultivar description


2020 ◽  
Vol 100 (5) ◽  
pp. 549-559
Author(s):  
Michelle Fraser ◽  
Stephen E. Strelkov ◽  
George D. Turnbull ◽  
Hafiz U. Ahmed ◽  
Wayne Barton ◽  
...  

Infection by Leptosphaeria maculans (Desm.) Ces. & de Not., the causal agent of blackleg of canola (Brassica napus L.), can result in seedling death, foliar lesions, and the development of stem cankers on adult plants. Strobilurin fungicides such as pyraclostrobin are popular, as they are effective against a broad range of fungal pathogens, have novel target specificity, and translaminar activity within the plant. The seed treatment Prosper FX (carbathiin + trifloxystrobin + metalaxyl) and an experimental formulation BAS 720 F (pyraclostrobin + fluxapyroxad + metalaxyl) were evaluated for their efficacy in managing blackleg when applied alone or in combination with the foliar fungicides Priaxor (pyraclostrobin + fluxapyroxad) or Tilt 250 EC (propiconazole). Experiments were conducted with the blackleg-susceptible cultivar ‘Westar’ and the moderately resistant cultivar ‘Dekalb 73-15 RR’ under greenhouse and field conditions in 2012 and 2013. In one greenhouse trial, all fungicide treatments significantly reduced stem blackleg severity on ‘73-15’ and ‘Westar’ compared with the inoculated–untreated controls. During 3 of 4 site-years, the combination of the experimental seed treatment BAS 720 F and Priaxor significantly reduced stem disease severity relative to the inoculated–untreated control by 60%–77% for ‘73-15’ and by 50%–75% for ‘Westar’. This treatment also resulted in a significant yield increase relative to the inoculated–untreated control in ‘Westar’ in 2 site-years by 24% and 56%. The results of the study suggest a reduction in blackleg symptoms and improved yields when canola is treated with a combination of seed and foliar fungicides, especially when cropping a susceptible cultivar.


1985 ◽  
Vol 65 (4) ◽  
pp. 889-892 ◽  
Author(s):  
IAN GRANT ◽  
WALLACE D. BEVERSDORF

The agronomic performance of eight triazine-resistant single-cross oilseed rape (B. napus L.) hybrids was evaluated. The yield of most of the hybrids was significantly greater than Atr-Regent; some of the hybirds demonstrated mid-parent heterosis, but none of the eight exhibited high-parent heterosis. In most cases the hybrids were intermediate to the parents for flowering date, plant height, lodging resistance, physiological maturity, percent oil and percent protein.Key words: Brassica napus L., oilseed rape, F1 hybrid, triazine resistance


Botany ◽  
2014 ◽  
Vol 92 (11) ◽  
pp. 847-853 ◽  
Author(s):  
Benjamin R. Montgomery ◽  
Maia F. Bailey ◽  
Gregory G. Brown ◽  
Lynda F. Delph

Gynodioecy frequently results from the interplay of mitochondrial cytoplasmic male sterility (CMS) and nuclear fertility-restoration genes. Models suggest that maintaining cytonuclear gynodioecy requires that restorer genes incur a cost to fitness because otherwise they would increase toward fixation. Direct tests of costs of restorer alleles require knowledge of the underlying genetics of sex determination. We use a well characterized CMS system in Brassica napus L. to measure aspects of fitness in four lineages that vary in whether they carry the pol CMS gene or male-fertile cytoplasm (cam), and whether they carry the Rfp restorer of pol or Rfn restorer of the nap CMS gene. As expected, plants with pol CMS and only the Rfn restorer experienced reduced flower size, stamen length, and pollen counts. Plants with pol and the Rfp restorer showed incomplete restoration with shorter stamens than both lines with cam cytoplasm and reduced pollen counts compared with plants with cam cytoplasm and the Rfp restorer. Among plants with cam cytoplasm, pollen counts were higher for those with the Rfp than Rfn restorer, indicating a greater cost of restoration associated with Rfn. These results demonstrate that costs of restoration differ for the Rfn and Rfp alleles in B. napus.


1983 ◽  
Vol 63 (2) ◽  
pp. 407-413 ◽  
Author(s):  
J. L. SERNYK ◽  
B. R. STEFANSSON

The degree of heterosis for seed yield in F1 hybrids of summer rape (Brassica napus L.) was examined in replicated yield trials during 2 yr (1980 and 1981) using intervarietal hybrids produced by manual crossing. The seed yields from the F1 hybrids of crosses between Marnoo and Regent, and Karat and Regent exceeded those of Regent by 38 and 43%, respectively. With the possible exception of maturity, which was 1 day later than Regent, the agronomic and quality characteristics of these hybrids appeared to be within the ranges acceptable in commercial rapeseed cultivars. However, the successful development of hybrid rapeseed cultivars still depends upon the development of a suitable cytoplasmic, genetic or chemical (male gametocide) pollination control system.Key words: Brassica napus L., canola, intervarietal hybrids


Sign in / Sign up

Export Citation Format

Share Document