Development and application of a method to classify airborne pollen taxa concentration using light scattering data

Although automated pollen monitoring networks using laser optics are well-established in Japan, it is thought that these methods cannot distinguish between pollen counts when evaluating various pollen taxa. However, a method for distinguishing the pollen counts of two pollen taxa was recently developed. In this study, we applied such a method to field evaluate the data of the two main allergens in Japan, Chamaecyparis obtusa and Cryptomeria japonica. We showed that the method can distinguish between the pollen counts of these two species even when they are simultaneously present in the atmosphere. This result indicates that a method for automated and simple two pollen taxa monitoring with high spatial density can be developed using the existing pollen network.

Distribution of Marine Palynomorphs in Surface Sediments, Prydz Bay, Antarctica

<p><b>Prydz Bay Antarctica is an embayment situated at the ocean-ward end of the LambertGlacier/Amery Ice Shelf complex East Antarctica. This study aims to document thepalynological assemblages of 58 surface sediment samples from Prydz Bay, and tocompare these assemblages with ancient palynomorph assemblages recovered fromstrata sampled by drilling projects in and around the bay.</b></p> <p>Since the early Oligocene, terrestrial and marine sediments from the Lambert Grabenand the inner shelf areas in Prydz Bay have been the target of significant glacialerosion. Repeated ice shelf advances towards the edge of the continental shelfredistributed these sediments, reworking them into the outer shelf and Prydz ChannelFan. These areas consist mostly of reworked sediments, and grain size analysisshows that finer sediments are found in the deeper parts of the inner shelf and thedeepest areas on the Prydz Channel Fan. Circulation within Prydz Bay is dominatedby a clockwise rotating gyre which, together with coastal currents and ice bergploughing modifies the sediments of the bay, resulting in the winnowing out of thefiner component of the sediment.</p> <p>Glacial erosion and reworking of sediments has created four differing environments(Prydz Channel Fan, North Shelf, Mid Shelf and Coastal areas) in Prydz Bay whichis reflected in the palynomorph distribution. Assemblages consist of Holocenepalynomorphs recovered mostly from the Mid Shelf and Coastal areas and reworkedpalynomorphs recovered mostly from the North Shelf and Prydz Channel Fan. Thepercentage of gravel to marine palynomorph and pollen counts show a relationshipwhich may reflect a similar source from glacially derived debris but the percentageof mud to marine palynomorph and pollen counts has no relationship.</p> <p>Reworked palynomorphs consist of Permian to Eocene spores and pollen and Eocenedinocysts which are part of the Transantarctic Flora. Holocene components are avaried assemblage of acritarchs, dinoflagellate cysts (dinocysts), prasinophyte algae,red algae and large numbers of Zooplankton sp. and foraminifera linings. In situdinocysts are dominated by the heterotroph form Selenopemphix antarctica and none of the Holocene dinocyst species found in Prydz Bay have been recorded in theArctic. In contrast acritarchs, prasinophytes and red algae are all found in the Arcticand reflect a low salinity and glacial meltwater environment. Comparison withmodern surface samples from the Arctic and Southern Ocean show there is a strongcorrelation to reduction in the autotroph:heterotroph dinocyst ratio with increasinglatitude.</p> <p>Todays assemblage of marine palynomorphs are more complex than those recordedin ancient assemblages and there is a lower level of reworked material. Acritarchs(Leiosphaeridia spp. Sigmopollis sp.) and prasinophytes (Cymatiosphaera spp.</p> <p>Pterospermella spp. Tasmanites spp.) are recorded in the ancient record in Antarcticaas well as surface sediments in Prydz Bay, but there are very low numbers ofLeiosphaeridia spp. and Sigmopollis spp. present today in comparison to the ancientrecord. Dinocysts in situ and recovered in Prydz Bay are endemic to the Antarcticbut have not been recorded in the ancient record.</p>

Distribution of Marine Palynomorphs in Surface Sediments, Prydz Bay, Antarctica

<p><b>Prydz Bay Antarctica is an embayment situated at the ocean-ward end of the LambertGlacier/Amery Ice Shelf complex East Antarctica. This study aims to document thepalynological assemblages of 58 surface sediment samples from Prydz Bay, and tocompare these assemblages with ancient palynomorph assemblages recovered fromstrata sampled by drilling projects in and around the bay.</b></p> <p>Since the early Oligocene, terrestrial and marine sediments from the Lambert Grabenand the inner shelf areas in Prydz Bay have been the target of significant glacialerosion. Repeated ice shelf advances towards the edge of the continental shelfredistributed these sediments, reworking them into the outer shelf and Prydz ChannelFan. These areas consist mostly of reworked sediments, and grain size analysisshows that finer sediments are found in the deeper parts of the inner shelf and thedeepest areas on the Prydz Channel Fan. Circulation within Prydz Bay is dominatedby a clockwise rotating gyre which, together with coastal currents and ice bergploughing modifies the sediments of the bay, resulting in the winnowing out of thefiner component of the sediment.</p> <p>Glacial erosion and reworking of sediments has created four differing environments(Prydz Channel Fan, North Shelf, Mid Shelf and Coastal areas) in Prydz Bay whichis reflected in the palynomorph distribution. Assemblages consist of Holocenepalynomorphs recovered mostly from the Mid Shelf and Coastal areas and reworkedpalynomorphs recovered mostly from the North Shelf and Prydz Channel Fan. Thepercentage of gravel to marine palynomorph and pollen counts show a relationshipwhich may reflect a similar source from glacially derived debris but the percentageof mud to marine palynomorph and pollen counts has no relationship.</p> <p>Reworked palynomorphs consist of Permian to Eocene spores and pollen and Eocenedinocysts which are part of the Transantarctic Flora. Holocene components are avaried assemblage of acritarchs, dinoflagellate cysts (dinocysts), prasinophyte algae,red algae and large numbers of Zooplankton sp. and foraminifera linings. In situdinocysts are dominated by the heterotroph form Selenopemphix antarctica and none of the Holocene dinocyst species found in Prydz Bay have been recorded in theArctic. In contrast acritarchs, prasinophytes and red algae are all found in the Arcticand reflect a low salinity and glacial meltwater environment. Comparison withmodern surface samples from the Arctic and Southern Ocean show there is a strongcorrelation to reduction in the autotroph:heterotroph dinocyst ratio with increasinglatitude.</p> <p>Todays assemblage of marine palynomorphs are more complex than those recordedin ancient assemblages and there is a lower level of reworked material. Acritarchs(Leiosphaeridia spp. Sigmopollis sp.) and prasinophytes (Cymatiosphaera spp.</p> <p>Pterospermella spp. Tasmanites spp.) are recorded in the ancient record in Antarcticaas well as surface sediments in Prydz Bay, but there are very low numbers ofLeiosphaeridia spp. and Sigmopollis spp. present today in comparison to the ancientrecord. Dinocysts in situ and recovered in Prydz Bay are endemic to the Antarcticbut have not been recorded in the ancient record.</p>

‘Pollen potency’: the relationship between atmospheric pollen counts and allergen exposure

Pollen allergies are responsible for a considerable global public health burden, and understanding exposure is critical to addressing the health impacts. Atmospheric pollen counts are routinely used as a predictor of risk; however, immune responses are triggered by specific proteins known as allergens, which occur both within and on the surface of the pollen grain. The ratio between atmospheric pollen counts and allergen concentrations (‘pollen potency’) has been shown to be inconsistent, with potentially important implications for pollen monitoring practice. Despite this, there has been no previous synthesis of the literature and our understanding of the factors that influence pollen potency remains poor. We conducted a scoping review with the aim of deriving a current understanding of: (a) the factors that influence pollen potency; (b) its variation through time, between taxa and by location; and (c) the implications for pollen monitoring practice. Our synthesis found that pollen potency is highly variable within and between seasons, and between locations; however, much of this variability remains unexplained and has not been deeply investigated. We found no predictable pollen potency patterns relating to taxon, geography or time, and inconclusive evidence regarding possible driving factors. With respect to human health, the studies in our synthesis generally reported larger associations between atmospheric allergen loads and allergy symptoms than whole pollen counts. This suggests that pollen potency influences public health risk; however, the evidence base remains limited. Further research is needed to better understand both pollen potency variability and its implications for health.

1250Grass pollen exposure and children’s asthma repeat admissions in Victoria, Australia

Background Paediatric asthma-related readmissions significantly challenge public health systems. As evidence suggests readmissions are possibly associated with ambient grass pollen levels, we sought to determine the relationship between grass pollen exposure and the risk of readmission for asthma among children in Victoria, Australia. Methods The Victorian Admitted Episodes Dataset 1st July 1997–30th June 2009 was used to identify primary admissions with a principal diagnosis of asthma for children aged 2-18 years. Total sample size was 48,068 admissions. Readmissions were defined as a subsequent admission within 28 days of index discharge. Multiple semi-parametric Poisson Generalized Additive Models were used to assess associations between the grass pollen season and daily grass pollen counts (lagged and cumulative) with repeat admissions, while controlling for meteorological factors and air pollutants. Results Readmissions totalled to 2,152 (4.47% of all admissions). Mean daily readmissions were 1.44 times higher during the grass pollen season (95%CI 1.03-2.02). Pollen season was associated with readmission only for the youngest age group (2-5), incident rate ratio 1.99 (95%CI 1.26-3.14). Daily readmission was non-linearly associated with same day pollen counts only for the 13-18-year age group. Lag 2 pollen counts were non-linearly associated with all daily readmissions for boys in gender-stratified and younger age groups (2-5, 6-12) in age-stratified analysis. Conclusions Grass pollen exposure was associated with higher readmission rates for paediatric asthma. Key messages Younger children and boys with severe or poorly controlled asthma need to be assessed for preventive therapies before the grass pollen season to reduce the repeat admissions.

Airborne Pollens and their Association with Meteorological Parameters in the Atmosphere of Shiraz, Southwest Iran

Airborne pollen is considered one of the causative agents of hay fever, allergic rhinitis, conjunctivitis, and asthma. We aimed to investigate airborne pollens in the context of Shiraz located in the southwest of Iran and find their association with meteorological parameters. The survey was conducted from October 2017 to September 2018, using seven days of volumetric Burkard spore trap, located in the center of the city. A total of 5810 pollen grains/m3 belonging to 15 taxa were identified and recorded. Among them, 73.8% was the tree, while the grass, shrub, and weed constituted 13.56%, 3.5%, and 9.2% of total reported pollens, respectively. The major pollen types were Platanaceae (28.39%), Oleaceae (21.17%), Pinaceae (15.11%), Amaranthaceae (9.29%), and Brassicaceae (8.02%). A higher number of pollen counts and types were recorded in March, followed by September, while it was lower in May. Meteorological parameters were correlated with the monthly pollen counts. Wind speed was found to have a positive correlation with Platanaceae concentration. The significant correlation between pollen concentration and the temperature was positive for Poaceae, Amaranthaceae, and Plantaginaceae and negative for Rosaceae, Oleaceae, and Ulmaceae. Poaceae and Amaranthaceae were negatively correlated with humidity and positively with Rosaceae, Oleaceae, and Plantaginaceae. A negative correlation was found between rainfall and Poaceae and Amaranthaceae, while Plantaginaceae had a positive correlation with this parameter. The results of this study may be helpful for allergologists in the diagnosis and treatment of airborne allergic disorders due to pollen grains.

Personalized Pollen Monitoring and Symptom Scores: A Feasibility Study in Grass Pollen Allergic Patients

Background: Pollen is a major trigger for allergic symptoms in sensitized individuals. Airborne pollen is usually monitored by Hirst type pollen samplers located at rooftop level, providing a general overview of the pollen distribution in the larger surroundings. In this feasibility study, grass pollen-sensitized subjects monitored the pollen in their direct environment using a portable pollen sampler (Pollensniffer) and scored their symptoms, to study the relation between symptom severity and personal grass pollen exposure. For comparison the symptoms were also correlated with pollen collected by the rooftop sampler.Methods: After recruitment 18 participants were screened for grass pollen specific (GP-sIgE) of which 12 were eligible. Nine participants completed the study (May, 2018). They were asked to monitor personal pollen exposure using a Pollensniffer on their way to school, work or other destination, and to score their symptoms via a mobile app on a scale from 0 to 10. Daily pollen concentrations were collected by a Hirst type sampler at rooftop level. Pollen grains were analyzed using a microscope.Results: Three of the four participants with high GP-sIgE (≥9.6 kU/l) reported high symptom scores (&gt;4) and an analysis showed a significant correlation (CC) between eye, nose, and lung symptoms and the grass pollen counts collected by the Pollensniffer, as well as the daily grass pollen concentrations monitored by the rooftop sampler (CC≥0.54). In contrast, the participants with low GP-sIgE levels (&lt;9.6 kU/l) reported low symptom scores (≤4) and often other sensitizations were present. For these subjects, no significant positive correlations (CC&lt;0.3) of symptoms with either grass pollen collected by the personal or the rooftop sampler were found.Conclusion: The results of this feasibility study suggest that correlations between the severity of clinical symptoms of grass pollen allergic patients, and grass pollen counts as determined by the Pollensniffer or a rooftop sampler, is restricted to patients with high GP-sIgE levels, high symptom scores, and no relevant other sensitizations. Based on the low numbers of subjects with severe symptoms included in this feasibility study, no conclusions can be drawn on the performance of the Pollensniffer in relating symptoms and pollen exposure in comparison with the rooftop sampler.Trial Registration: The study was approved by the Committee Medical Ethics of the LUMC (approval numbers: NL63953.058.17/ P17.304).

Pollen in Dust

Twenty-eight pairs of pollen and aeolian dust traps covering the State of Kuwait were used to obtain seasonal pollen counts of the most eight dominant families during 2009–2011 i.e. Chenopodiaceae, Poaceae (Gramineae), Cyperaceae, Leguminosae (Fabaceae), Cyperaceae, Brassicaceae, Malvaceae, Compositae, and Plantaginaceae. The sampling site locations were chosen to cover all the geomorphological sectors and native vegetation areas in Kuwait. Generally, pollen counts show us a remarkable distinction between the year 2009–2010 and 2010–2011, It is also evident that pollen counts (concentration) vary from season to season. Pollen counts over four seasons for two years (October 2009–August 2011) reveal the presence of two peaks in spring (April–May) and autumn (October–November). Map distribution of pollens in each of the dominant plant families in Kuwait is generated according to seasons showing higher and lower concentrations of dust pollen counts.

Compilation of relative pollen productivity (RPP) estimates and taxonomically harmonised RPP datasets for single continents and Northern Hemisphere extratropics

Relative pollen productivity (RPP) estimates are fractionate values, often in relation to Poaceae, that allow vegetation cover to be estimated from pollen counts with the help of models. RPP estimates are especially used in the scientific community in Europe and China, with a few studies in North America. Here we present a comprehensive compilation of available northern hemispheric RPP studies and their results arising from 51 publications with 60 sites and 131 taxa. This compilation allows scientists to identify data gaps in need of further RPP analyses but can also aid them in finding an RPP set for their study region. We also present a taxonomically harmonised, unified RPP dataset for the Northern Hemisphere and subsets for North America (including Greenland), Europe (including arctic Russia), and China, which we generated from the available studies. The unified dataset gives the mean RPP for 55 harmonised taxa as well as fall speeds, which are necessary to reconstruct vegetation cover from pollen counts and RPP values. Data are openly available at https://doi.org/10.1594/PANGAEA.922661 (Wieczorek and Herzschuh, 2020).