Comprehensive Investigation of a Toxic Microcystis sp. (Cyanobacteria) Collected from Snow Lake (New Mexico, USA).

Mapping Intimacies ◽

10.21203/rs.3.rs-1131402/v1 ◽

2021 ◽

Author(s):

Sergei Shalygin ◽

Omar Holguin

Keyword(s):

New Mexico ◽

Emission Spectra ◽

Composition Analysis ◽

Cell Content ◽

Spectra Analysis ◽

Mass Spectral ◽

Pigment Distribution ◽

Taxonomic Affiliation ◽

Molecular Sequencing ◽

Derivatives Of

Abstract The toxin producing cyanobacterium Microcystis sp. was collected in the mid October 2020 from the shallow waters of Snow Lake (New Mexico, USA). This species caused a visible bloom consisting of the pale green irregular macro colonies. Mass spectral analysis of the biomass revealed the presence of 4 derivatives of microcystin in that bloom: MC-LR (in the water and biomass), MC-RR (in biomass), MC-LY (in biomass), and MC-YR (in biomass).Next-generation sequencing allowed the retrieval of two Microcystis sequences in the bloom; which are molecular benchmarks for toxic Microcystis that may be used in future monitoring studies. Light microscopy provided evidence for the taxonomic affiliation of the found morphotype as Microcystis flos-aquae (Wittrock) Kirchner. However, molecular sequencing and the present situation in cyanobacterial taxonomy prevented affiliation of our morphotype to Microcystis flos-aquae, justifying following name – Microcystis sp. Confocal microscopy was used to determine the distribution of the cell content utilizing 3D stereo imaging. Emission spectra analysis identified the pigment composition and pigment distribution within the cells. SEM revealed 3D arrangement of the cells in the colonies, texture of the surface of the cells (perhaps dehydrated collapsed polysaccharides), F-layer and pili-like structures. Additionally, SEM/EDS analysis confirmed the F-layer using elemental composition analysis, which showed sulfur in the F-layer – typical element for that structure. Through the use of AFM, we analyzed the texture of the cell's surface and confirmed pili-like structures.

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Interaction of Fullerenes with Organosilanes in the Ionization Chamber of a Mass Spectrometer under Electron Impact and the Reaction of C60with Tetraphenylsilane in Solution under UV Irradiation

Journal of Spectroscopy ◽

10.1155/2016/8158623 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9

Author(s):

Yury I. Lyakhovetsky ◽

Elena A. Shilova ◽

Alexandra P. Pleshkova ◽

Alexander I. Belokon ◽

Sergey O. Yakushin ◽

...

Keyword(s):

Electron Impact ◽

Mass Spectrometer ◽

Uv Irradiation ◽

Ionization Chamber ◽

Ion Source ◽

Organic Radicals ◽

Mechanistic Study ◽

Mass Spectral ◽

Additional Support ◽

Derivatives Of

C60was shown to react with organosilanes Me4Si, Ph2SiH2, Ph2MeSiH, Ph4Si, andα-naphthylphenylmethylsilane in the electron ionization ion source of a mass spectrometer with the transfer of the corresponding organic radicals (Me, Ph, andα-naphthyl) from the silanes to the fullerene. The reactions were accompanied by hydrogen addition to some products and hydrogen loss from them. C70reacted with Me4Si analogously. A reaction mechanism involving homolytic dissociation of the silanes under electron impact to the corresponding organic radicals, which react further with C60at the surface of the ionization chamber of the mass spectrometer to give the respective adducts, was offered. A mechanistic study of the reaction of C60with Me4Si supported it. No silicon containing derivatives of the fullerenes were found. C60reacted with Ph4Si in solution under UV irradiation in a similar fashion furnishing phenyl derivatives of the fullerene. These results provide an additional support to the hypothesis formulated earlier thatthe homolytic reactive mass spectrometry of fullerenes (the reactions of fullerenes with other species in the ionization chambers of mass spectrometers and their mass spectral monitoring)can predict the reactivity of them toward the same reagents in solution to a significant extent.

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Massenspektrometrische Fragmentierung von Trimethylsilylderivaten (TMS) des Thyrotropin-„Releasing“-Hormons (TRH) und dessen Teilstrukturen / Mass Spectroscopy of Trimethylsilyl Derivatives of Thyrotropin-releasing Hormone and its Constituents

Zeitschrift für Naturforschung B ◽

10.1515/znb-1973-11-1222 ◽

1973 ◽

Vol 28 (11-12) ◽

pp. 820-823 ◽

Cited By ~ 4

Author(s):

W. A. König ◽

S. Fuchs ◽

K. Zech ◽

W. Voelter

Keyword(s):

Mass Spectrometry ◽

Mass Spectroscopy ◽

Peptide Hormones ◽

Thyrotropin Releasing Hormone ◽

Mass Spectral Fragmentation ◽

Releasing Hormone ◽

Mass Spectral ◽

Trimethylsilyl Derivatives ◽

Fragmentation Patterns ◽

Derivatives Of

The mass spectral fragmentation patterns of trimethylsilyl derivatives of TRH and its constituents are discussed. The utility of these derivatives for the control of the synthesis of peptide hormones by mass spectrometry is obvious.

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Preparation and Spectroscopic Characterisation of a Series of Heterobimetallic N-phenyldiethanolaminate-alkoxide Derivatives of Oxovanadium(V)

Journal of Chemical Research ◽

10.3184/0308234054506857 ◽

2005 ◽

Vol 2005 (6) ◽

pp. 352-355 ◽

Cited By ~ 3

Author(s):

Rajendra Singh Ghadwal ◽

Ram C. Mehrotra ◽

Anirudh Singh

Keyword(s):

Molecular Weight ◽

Spectral Studies ◽

Metal Alkoxides ◽

Nmr Studies ◽

Mass Spectral ◽

Elemental Analyses ◽

Derivatives Of ◽

Spectroscopic Characterisation

Reaction of VO(OPri)3 with two equivalents of N-phenyldiethanolamine (PhDEAH2) yields a homometallic complex [VO(PhDEA)(PhDEAH)] 1, which reacts with a number of metal alkoxides to afford heterobimetallic oxovanadium(V) complexes of the types [VO(PhDEA)2{M(OR)n-1}] [where PhDEA = C6H5N(CH2CH2O–)2], [M =: Al (n = 3, R = Pri) 2; Al (n = 3, R = But) 3; Ti (n = 4, R = Pri) 4; Zr (n = 4, R = Pri) 5; Nb (n = 5, R = Pri) 6; Ta (n = 5, R = Pri) 7. The derivative [VO(PhDEA)(OSiPh3)] 8 has been prepared by the equimolar interaction of [VO(PhDEA)(OPri)] with Ph3SiOH. All of these complexes have been characterised by spectroscopic (IR; 1H, 13C, 27Al and 51V NMR) studies, elemental analyses, and molecular weight measurements. The derivative 2 has also been characterised by FAB mass spectral studies, which supports for its monomeric nature.

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E.I. and C.I. mass-spectral identification of some derivatives of 7-O-(2-amino-2-deoxy-α-d-glucopyranosyl)-l-glycero-d-manno-heptose, obtained from lipopolysaccharides representative of the Vibrionaceae family

Carbohydrate Research ◽

10.1016/0008-6215(84)85330-6 ◽

1984 ◽

Vol 128 (2) ◽

pp. 203-216 ◽

Cited By ~ 19

Author(s):

Joseph H. Banoub ◽

Francis Michon ◽

Derek H. Shaw ◽

René Roy

Keyword(s):

Mass Spectral ◽

Spectral Identification ◽

Derivatives Of

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Mass spectral fragmentation of perfluoroacyl derivatives of half nitrogen mustards for their detection by gas chromatography/mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.8777 ◽

2020 ◽

Vol 34 (12) ◽

Author(s):

Buddhadeb Chandra ◽

Kanchan Sinha Roy ◽

Mahabul Shaik ◽

Chandrakant Waghmare ◽

Meehir Palit

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Gas Chromatography Mass Spectrometry ◽

Nitrogen Mustards ◽

Mass Spectral Fragmentation ◽

Mass Spectral ◽

Chromatography Mass Spectrometry ◽

Derivatives Of

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Spectroscopic Studies of Copper and Silver Binding to Metallothioneins

Metal-Based Drugs ◽

10.1155/mbd.1999.277 ◽

1999 ◽

Vol 6 (4-5) ◽

pp. 277-290 ◽

Cited By ~ 11

Author(s):

Martin J. Stillman

Keyword(s):

Spectral Data ◽

Metal Binding ◽

Emission Spectra ◽

Structural Data ◽

Spectroscopic Studies ◽

Yeast Cells ◽

Luminescence Spectra ◽

Ionization Mass ◽

Mass Spectral ◽

Metal Free

Mammalian metallothionein is remarkable in its metal binding properties: well-characterized species exist for metal to sulfur ratios of M7S20, M12S20, and M18S20, where M = Cd(ll), Zn(ll), Hg(ll), Ag(I), Au(I), and Cu(I). Circular dichroism and luminescence spectra provide rich details of a complicated metal binding chemistry when metals are added directly to the metal free- or zinc-containing protein. CD spectral data unambiguously identify key metal to protein stoichiometric ratios that result in well-defined structures. Emission spectra in the 450-750 nm region have been reported for metallothioneins containing Ag(I), Au(I), and Cu(I). The luminescence of Cu-MT can also be detected directly from mammalian and yeast cells. Qualitative and quantitative interpretations show that the final structure adopted by Ag-MT is not the same as that formed by Cu(I) ions in Cu-MT. XAFS structural data are reported for a number of metallothioneins, including Ag12-MT and Ag17-MT. Electrospray ionization mass spectrometry provides details on the species formed when Ag(I) binds to metallothionein. Mass spectral data are reported for metal-free MT 2A and Agn-MT (n = 14-18).

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Emission spectra/analysis

Spectrochimica Acta ◽

10.1016/0371-1951(50)80028-0 ◽

1950 ◽

Vol 4 (1) ◽

pp. 69

Keyword(s):

Emission Spectra ◽

Spectra Analysis

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Synthesis, Spectral and Microbial Studies of Some Novel Schiff Base Derivatives of 4-Methylpyridin-2-amine

E-Journal of Chemistry ◽

10.1155/2009/247209 ◽

2009 ◽

Vol 6 (4) ◽

pp. 1205-1210 ◽

Cited By ~ 3

Author(s):

J. J. Vora ◽

S. B. Vasava ◽

K. C. Parmar ◽

S. K. Chauhan ◽

S. S. Sharma

Keyword(s):

Schiff Base ◽

Antimicrobial Activities ◽

Dilution Method ◽

Mass Spectral ◽

H Nmr ◽

Schiff Base Derivatives ◽

Ft Ir ◽

Acid Catalyzed ◽

Derivatives Of ◽

Broth Dilution

Schiff base derivatives ofN-{(1E)-[3-(mono or di-substituted aryl)-1-phenyl-1H-pyrazol-4-yl]methylene{-4-methylpyridin-2-amine were synthesized by the acid catalyzed condensation of 3-(mono- or di- substituted aryl)-1-phenyl-1H-pyrazole-4-carbaldehyde derivatives with 4-methylpyridin-2-amine. Schiff base derivatives were characterized by FT-IR,1H-NMR, Mass spectral analysis and elemental analysis. All the synthesized compounds have been screened for their antimicrobial activities by using broth dilution method.

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