Transgenic Elite Lines of Carioca Seeded Common Bean (Phaseolus Vulgaris L.) With Multiple Resistance to Viruses Reduce Cowpea Mild Mottle Virus Transmission

Abstract The most important viruses infecting common bean (Phaseolus vulgaris L.) in Brazil are BCMV, BGMV and CPMMV, the last two transmitted by the whitefly Bemisia tabaci, occurring simultaneously and causing severe yield losses. Genetically modified progenies of common bean, from carioca market class and multiple virus resistance (BCMV, BGMV and CPMMV), have been developed using conventional breeding and molecular tools. Agronomic performance and virus disease severity (VS) evaluated in two field trials, selected 39 elite progenies out of 477. Molecular analyses identified the presence of BCMV and BGMV resistance alleles in plants. CPMMV resistance was measured on mechanically inoculated plants using a VS scoring scale. Among the lowest VS average scores, five progenies showed resistance to BCMV, BGMV and CPMMV, and upright plant architecture, resistance to plant lodging and carioca market class grains, presenting potential to be developed into a new transgenic cultivar, with multiple virus resistance. Additionally, the resistant progenies may also contribute to reduce virus spread in the field, as they were a less efficient inoculum source of CPMMV in insect transmission assays.

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A Test Cross Protocol for Determining the Genotype of Dark Red Seedcoat Colors in Common Bean

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.123.6.1048 ◽

1998 ◽

Vol 123 (6) ◽

pp. 1048-1052 ◽

Cited By ~ 3

Author(s):

Mark J. Bassett

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Dominant Gene ◽

Breeding Lines ◽

Test Cross ◽

Red Color ◽

Genetic Stocks ◽

Market Class ◽

Red Bean

The red common bean (Phaseolus vulgaris L.) seedcoat colors produced by the dominant gene R and the dark red kidney gene rkd are very similar, making it difficult for breeders of red bean varieties to know which genotype is in their materials. A protocol employing test crosses with genetic stocks having known genotypes for seedcoat colors was developed to identify genotypes with either of two very similar dark red seedcoat colors: garnet brown controlled by rkd and oxblood controlled by R. Twenty bean varieties and breeding lines were test crossed with genetic tester stocks cu BC3 5-593 and b v BC3 5-593, and four of the varieties were test crossed with [? R] b v BC3 5-593. Analysis of the seedcoat colors and patterns in the F1 progenies from the test crosses demonstrated that unambiguous identification of the genotypes of the two dark red colors could be achieved using the cu BC3 5-593 and b v BC3 5-593 testers. The dark red color (garnet brown) of the Small Red market class materials was demonstrated to be produced by rkd, and the dark red color (oxblood) of `Jacobs Cattle' was demonstrated to be produced by R. A Light Red Kidney market class stock was derived from `Redkloud' and used in two crosses: cu b v rk BC1 5-593 × b v BC3 5-593 and cu b v rk BC1 5-593 × cu BC3 5-593. Classification of the F2 progenies demonstrated that the cu gene does not entirely prevent rk red color from being modified by V. The interactions of rk, rkd, and R with C, cu, G, B, and V are discussed, and previous literature concerning those interactions is critically reviewed.

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The Seedcoat Color Genotype of `Prim' and the Manteca and Coscorrón Market Classes of Common Bean

HortScience ◽

10.21273/hortsci.34.2.336 ◽

1999 ◽

Vol 34 (2) ◽

pp. 336-337 ◽

Cited By ~ 3

Author(s):

Mark J. Bassett

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Recurrent Parent ◽

Anecdotal Evidence ◽

Dry Bean ◽

Genetic Interpretation ◽

Market Class

Anecdotal evidence exists for nonflatulence among Chilean Manteca and Coscorrón market classes of common bean (Phaseolus vulgaris L.), and there is an hypothesis that the seedcoat color may be associated with superior digestibility. The inheritance of seedcoat color in `Prim', a Manteca market-class dry bean, was investigated using a protocol employing genetic interpretation of seedcoat colors in the F1 from testcrosses of `Prim' with a series of tester stocks. Most of the genetic tester stocks were constructed previously by backcrossing selected recessive alleles for seedcoat color into a recurrent parent (5-593) with seedcoat color genotype P [C r] D J G B V Rk Asp. The genetic tester stocks included two varieties, `Masterpiece' and `V0687', and testers constructed on the 5-593 background, viz., j BC2 5-593, d j BC2 5-593, asp BC2 5-593, b v BC2 5-593, v BC2 5-593, and cu BC3 5-593. The seedcoat color genotype of `Prim' was found to be P [C r] d j G b vlae. The implications of this genotype for pigment chemistry are discussed.

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Developing cultivars of the common bean (Phaseolus vulgaris L.) for southern Africa: bean common mosaic virus resistance, consumer preferences and agronomic requirements

Field Crops Research ◽

10.1016/0378-4290(94)00101-h ◽

1995 ◽

Vol 40 (3) ◽

pp. 165-177 ◽

Cited By ~ 1

Author(s):

O.Z. Mukoko ◽

N.W. Galwey ◽

D.J. Allen

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Mosaic Virus ◽

Southern Africa ◽

Virus Resistance ◽

Consumer Preferences ◽

Bean Common Mosaic Virus ◽

Phaseolus Vulgaris L ◽

The Common

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Sensory changes related to breeding for plant architecture and resistance to viruses and anthracnose in bean market class Fabada (Phaseolus vulgaris L.)

Euphytica ◽

10.1007/s10681-011-0540-9 ◽

2011 ◽

Vol 186 (3) ◽

pp. 687-696 ◽

Cited By ~ 6

Author(s):

J. J. Ferreira ◽

Romero R. del Castillo ◽

E. Perez-Vega ◽

M. Plans ◽

J. Simó ◽

...

Keyword(s):

Phaseolus Vulgaris ◽

Plant Architecture ◽

Phaseolus Vulgaris L ◽

Market Class ◽

Sensory Changes ◽

Resistance To Viruses

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Comparative Mapping of Three Bacterial, Three Fungal, and One Virus Disease-resistance Genes in Common Bean

HortScience ◽

10.21273/hortsci.33.3.547a ◽

1998 ◽

Vol 33 (3) ◽

pp. 547a-547

Author(s):

Geunhwa Jung ◽

James Nienhuis ◽

Dermot P. Coyne ◽

H.M. Ariyarathne

Keyword(s):

Disease Resistance ◽

Common Bean ◽

Pseudomonas Syringae ◽

Resistance Genes ◽

Fungal Pathogens ◽

Xanthomonas Campestris ◽

Virus Disease ◽

Disease Resistance Genes ◽

Brown Spot ◽

Viral Pathogen

Common bacterial blight (CBB), bacterial brown spot (BBS), and halo blight (HB), incited by the bacterial pathogens Xanthomonas campestris pv. phaseoli (Smith) Dye, Pseodomonas syringae pv. syringa, and Pseudomonas syringae pv. phaseolicola, respectively are important diseases of common bean. In addition three fungal pathogens, web blight (WB) Thanatephorus cucumeris, rust Uromyces appendiculatus, and white mold (WM) Sclerotinia sclerotiorum, are also destructive diseases attacking common bean. Bean common mosaic virus is also one of most major virus disease. Resistance genes (QTLs and major genes) to three bacterial (CBB, BBS, and HB), three fungal (WB, rust, and WM), and one viral pathogen (BCMV) were previously mapped in two common bean populations (BAC 6 × HT 7719 and Belneb RR-1 × A55). The objective of this research was to use an integrated RAPD map of the two populations to compare the positions and effect of resistance QTL in common bean. Results indicate that two chromosomal regions associated with QTL for CBB resistance mapped in both populations. The same chromosomal regions associated with QTL for disease resistance to different pathogens or same pathogens were detected in the integrated population.

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