scholarly journals Chemical Composition and Biological Activity of Peucedanum Dhana A. Ham Essential Oil

2020 ◽  
Author(s):  
Sarunpron Khruengsai ◽  
Teerapong Sripa ◽  
Narawadee Rujanapun ◽  
Rawiwan Charoensup ◽  
Patcharee Pripdeevech
Keyword(s):  
Essential Oil ◽  
Cytotoxic Effect ◽  
Inhibitory Concentration ◽  
Dry Weight ◽  
Sw480 Cell ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Scavenging Activity ◽  
Total Phenolic Compounds ◽  
Functional Ingredients

Abstract The essential oil of Peucedanum dhana A. Ham growing in Thailand was investigated for their volatile compounds and screened for its antimicrobial, antioxidant, and cytotoxic activities. Forty-two compounds were identified, with the most important compounds being trans-piperitol, o-cymene, β-pinene, γ-terpinene, and limonene. Essential oil of P. dhana has remarkable antimicrobial activity against tested pathogens with minimum inhibitory concentration and minimum microbicidal concentration values, ranging from 62.50–250 µg/mL and 250-1,000 µg/mL, respectively. The antioxidant activity of P. dhana oil was measured using DPPH and ABTS scavenging activity assays. The IC50 values using the DPPH and ABTS method were 9.13 and 9.36 mg/mL, respectively. The total phenolic compounds in P. dhana oil was 183.05 mg GAE/100 g dry weight. The cytotoxic effect of P. dhana oil was tested against Hela, A549, SW480, and 3T3L1 cells. The essential oil had cytotoxicity against all cancer cells, with significant cytotoxicity towards the SW480 cell. The results indicate that the P. dhana essential oil could be used as a source of functional ingredients in food and pharmaceutical applications.

scholarly journals Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss. from Macedonia

2014 ◽  
Vol 66 (1) ◽  
pp. 401-413 ◽  
Author(s):  
Ksenija Mileski ◽  
Ana Dzamic ◽  
Ana Ciric ◽  
Slavica Grujic ◽  
M. Ristic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts ◽  
Positive Controls

This study was undertaken to determine the antioxidant and antimicrobial effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam. Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana essential oil was characterized by the presence of methyl eugenol (60.40%), p-cymene (11.18%) and ?-phellandrene (10.23%). The free radical scavenging activity of extracts and essential oil was evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger antioxidant activity compared to hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and ABTS tests, respectively. The total phenolic and flavonoid concentrations in the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The antimicrobial properties of the extracts and essential oil were investigated using a micro-well dilution technique against human pathogenic strains. The results were comparable with the effects of the positive controls, streptomycin and fluconazole. These findings indicate that E. sibthorpiana extracts and oil can be used in preventive treatments and as an alternative for synthetic preservatives.

Total Phenolic, Antioxidant Activities and Flavonoid Contents of Herbal Syrups

2019 ◽  
Vol 800 ◽  
pp. 60-64
Author(s):  
Fredijs Dimiņš ◽  
Ingrīda Augšpole
Keyword(s):  
Antiradical Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Total Phenolic Compounds ◽  
Trolox Equivalent

The aim of the study was to characterize antioxidative properties and antiradical activity of the herbal syrups collection in Latvia. Spectrophotometry was used to characterize antioxidant properties of herbal syrups. Antiradical activity, total phenols and content of flavonoids were determined spectrophotometrically. The antiradical scavenging activity was measured by the DPPH (2.2-diphenyl-1-picrylhydrazyl) reaction. The herbal syrups antiradical scavenging activity was estimated by the amount of DPPH of reagent using in reaction with a sample of vegetable syrup (in percent) and by Trolox equivalent. The total phenolic compounds were determined by using the Folin – Ciocalteu method. The total phenolic content was expressed in mg of the gallic acid equivalents. The content of flavonoids was determined by using reaction between the AlCl3 and flavonoids. Antiradical scavenging activity by Trolox equivalent of the herbal syrups was found from 81 to be 130 mg 100 g-1 herbal syrups. The content of total phenols was found from 22 to be 282 mg GAE 100 g-1, but content of total flavonoids by quercetin equivalent was found from 1 to 44 mg QE 100 g-1. In case of increased disease problems, syrups with increased antioxidant content should be used. However, the specificity of each disease should be taken into account as well as the content of certain compounds in the plants.

scholarly journals Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp

2020 ◽  
Vol 10 (17) ◽  
pp. 6007 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wen-Shin Lin ◽  
Jing-Wen Tung ◽  
Ya-Chih Cheng ◽  
Min-Yun Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Jujube Fruit

In this study, the effects of different fruit parts and extraction conditions on the antioxidant properties of jujube (Ziziphus jujuba Mill.) fruit were investigated. Five in vitro antioxidant models and statistical analyses were performed. The results revealed that jujube peel with pulp (peel pulp) exhibited better antioxidant capacity than did seeds. Overall, jujube peel pulp extracted using 50% ethanol at 60 °C exhibited the best antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (0.3 ± 0 mg/mL), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (0.5 ± 0 mg/mL), total phenolic content (38.3 ± 0.4 mg gallic acid equivalent per gram dry weight), total flavonoid content (43.8 ± 0.2 mg quercetin equivalent per gram dry weight), and reducing power (41.9 ± 2.2 mg ascorbic acid equivalent per gram dry weight). The results indicated that jujube peel pulp is a more potential natural antioxidant than seeds.

scholarly journals The Effects Of Wild Thyme (Thymus Serpyllum L.) Essential Oil Components Against Ochratoxin-Producing Aspergilli / Majčina Dušica (Thymus Serpyllum L.) I Njezine Komponente Protiv Okratoksikotvornih Vrsta Aspergillusa

2012 ◽  
Vol 63 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Darja Sokolić-Mihalak ◽  
Jadranka Frece ◽  
Anita Slavica ◽  
Frane Delaš ◽  
Hrvoje Pavlović ◽  
...  
Keyword(s):  
Essential Oil ◽  
Inhibitory Concentration ◽  
Phenolic Content ◽  
Fungal Growth ◽  
Inhibitory Effect ◽  
Aspergillus Ochraceus ◽  
Total Phenolic ◽  
Wild Thyme ◽  
Oil Components ◽  
Thymus Serpyllum

AbstractThe aim of this study was to determine the effects of the essential oil of Thymus serpyllum L. and of its components thymol and total phenols (total phenolic content, TPC) extracted from the plant on the growth and mycotoxin production of Aspergillus ochraceus, A. carbonarius, and A. niger. Minimal inhibitory concentration (MIC) determined for the essential oil and thymol, and selected concentration of the TPC extract inhibited fungal growth and ochratoxin A biosynthesis by more than 60 %, depending on the conditions and duration of incubation with the fungi. Essential oil showed the strongest inhibitory effect which may have been related to the synergistic or cumulative effects of its components

Essential Oil and Total Phenolic Compounds Variation inAchillea pachycephalaCollected from Different Regions of Iran

2012 ◽  
Vol 15 (2) ◽  
pp. 217-226 ◽  
Author(s):  
Mehdi Rahimmalek ◽  
Sayed Amir Hossein Goli ◽  
Nematolah Etemadi ◽  
Shima Gharbi
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Total Phenolic ◽  
Total Phenolic Compounds

scholarly journals Optimization of Ultrasound Assisted Extraction of Phenolic Compounds from Orange By-Product

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 49
Author(s):  
María del Carmen Razola-Díaz ◽  
Eduardo Jesús Guerra-Hernández ◽  
Celia Rodríguez-Pérez ◽  
Ana María Gómez-Caravaca ◽  
Belén García-Villanova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Orange Peel ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Quantitative Analyses ◽  
Ultrasound Assisted

Orange peel (OP) is the main by-product from orange juice industry. OP is a known source of bioactive compounds and is widely studied for its antioxidant, anti-inflammatory, anti-cancer, anti-rheumatic, anti-diabetic and cardioprotective activities. Thus, this research focuses on the establishments of ultrasound-assisted extraction of phenolic compounds in orange peel using a sonotrode; objective framed in the European SHEALTHY (non-thermal physical technologies to preserve healthiness of fresh and minimally processed fruit and vegetables) project. For this purpose, a Box Behnken design of 27 experiments was carried out with 4 independent factors (ratio ethanol/water, time (min), amplitude (%) and pulse (%)). Quantitative analyses of total phenolic compounds (TPC) were performed by Folin-Ciocalteu method and the antioxidant activity was measured by ABTS and DPPH methods. The validity of the experimental design was confirmed by ANOVA and the optimal sonotrode extraction conditions were obtained by response surface methodology. The optimal extracts were characterized by HPLC coupled to mass spectrometer detectors. The highest phenolic content and antioxidant activity was obtained using 45/55 ethanol/water (v/v), 35 min, amplitude 90% (110 W) and pulse 100%. The established method allows the extraction of 30.42 mg of gallic acid equivalents/g dry weight of total phenolic compounds from OP; this value suppose an increment up to 60% higher than conventional extraction.

scholarly journals Artemisia dracunculus Essential Oil Chemical composition and antioxidant properties

2019 ◽  
Vol 70 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Ariadna Petronela Fildan ◽  
Ioan Pet ◽  
Daniela Stoin ◽  
Gabriel Bujanca ◽  
Alexandra Teodora Lukinich-Gruia ◽  
...  
Keyword(s):  
Free Radical ◽  
Chemical Composition ◽  
Essential Oil ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Artemisia Dracunculus

Free radical scavenging activity, total phenolic content and the chemical composition of the essential oil isolated by steam distillation from Artemisia dracunculus L. was investigated. The isolation yield was 0.24% (v/w) based on the fresh plant material (leaves). GC-MS investigation identified 21 components, accounting 99.93% of the total amount. The major components were sabinene (42.38%), isoelemicin (12.91%), methyl eugenol (9.09%), elemicin (7.95%) and beta-ocimene (6.46%). The free radical scavenging activity of the essential oil of Artemisia dracunculus L. was evaluated in vitro by the DPPH assay (IC50 = 0.730 � 0.213 mg/mL), BHA and alpha-tocopherol were used as a positive control. The total phenolic content of the tarragon essential oil was evaluated by the Folin-Ciocalteu method (GAE = 0.451 � 0.001 mg/g sample). In view of these data, we consider that tarragon essential oil could represent a new antioxidants source as a reliable option to reduce the usage of synthetic additives.

scholarly journals Carotenoids, Flavonoids, Total Phenolic Compounds Content and Antioxidant Activity of Indigenous Pyracantha coccinea M. Roem. Fruits

2020 ◽  
Vol 71 (4) ◽  
pp. 258-266
Author(s):  
Dan Razvan Popoviciu ◽  
Ticuta Negreanu-Pirjol ◽  
Ludmila Motelica ◽  
Bogdan-Stefan Negreanu-Pirjol
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Dry Weight ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Methanol Extraction ◽  
Total Antioxidant ◽  
Fresh Frozen ◽  
Pyracantha Coccinea

Scarlet/red firethorn, Pyracantha coccinea M. Roem., is a common ornamental shrub, commonly cultivated nowadays in Romania and many other countries, in gardens or hedges. The tree has small white flowers and it produces small bright colored berries. The fruit is bitter and astringent, making it inedible when raw. The fruit can be cooked to make jellies, jams, sauces and marmalade. In this study, the Pyracantha coccinea M. Roem fruits from three common cultivars named �Orange Glow�, �Red Column� and �Soleil d�Or�, were collected from different individuals in Constanta city, Romania. Both frozen and dried (80�C) fruits were analyzed for the active principles content such as, carotenoids, flavonoids and total phenolic compounds correlated with antioxidant activity. Carotenoids were determined by acetone extraction and UV-Vis spectrophotometry, flavonoids, by methanol extraction and UV-Vis spectrophotometry and total phenolic, by methanol extraction, Folin-Ciocalteu reaction and UV-Vis spectrophotometry. The total antioxidant capacity was quantified through photochemiluminescence method by comparison with the standard substance used for calibration, Trolox� as tocopherol analogue (ACL procedure) using Photochem apparatus, Analytik Jena AG, Germany. The average carotenoid content in fresh frozen fruits ranged between 278-545 mg/kg (dry weight equivalent), while in dry material 133-203 mg/kg. Total flavonoids averaged 1415-4953 mg/kg in fresh fruits, increasing after heat-drying to 2981-5759 mg/kg. Fresh fruits contained a total amount of phenolic compounds of 11501-21107 mg/kg DW gallic acid equivalent, while dry fruits averaged 5749-8261 mg/kg GAE. Pyracantha coccinea M. Roem. fruits emphasized an increased antioxidant activity correlated with a high content of potentially bioactive compounds, especially of phenolic compounds, both in frozen and heat-dried state. �Red Column� and �Orange Glow� are the most promising varieties of the three cultivars, for potential pharmaceutical applications.

scholarly journals Influence of Germination Conditions and Parameters on the Content of Total Phenolic Compounds and Scavenging Activity in Germinated Seeds

2020 ◽  
Vol 44 (339) ◽  
pp. 46-53
Author(s):  
Ilze Bernate ◽  
Martins Sabovics ◽  
Lolita Tomsone
Keyword(s):  
Phenolic Compounds ◽  
Cannabis Sativa ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Phenolic Compounds ◽  
Beneficial Effects ◽  
Sprouted Seeds ◽  
Edible Seeds ◽  
Excellent Source ◽  
Germinated Seeds

AbstractConsumers are increasingly consuming sprouted seeds due to their low calories content, nutritional value, as well as beneficial effects on human health. Sprouts contain many bioactive compounds such as minerals, fibre, vitamin C, carotenoids and phenolic compounds. The aim of this study was to determine the effects of darkness, light and time total phenolic content and scavenging activity in alfalfa (Medicago sativa), radish (Raphanus sativus), broccoli (Brassica oleracea) and hemp (Cannabis sativa) seeds during germination. The seeds were washed, soaked in water by ratio of 2:1 (water : seeds) for 12±1 h and then germinated in light and dark conditions at a temperature of 22±2 °C and a relative humidity of 85±2% for different times (12, 24, 36 and 48 hours) in a climatic chamber ICH110 (Memmert, Germany). The quality changes of germinated seeds are determined by phenol content and scavenging activity. Un-germinated seeds were used as a control. The results of current studies show that after germination the highest increase in total phenols was in radish, broccoli and alfalfa seeds. The scavenging activity (SA) was higher after 48 hours of germination in all seed types compared to un-germinated seeds both in darkness and in light. This study shows that sprouted edible seeds are an excellent source of total phenolic compounds and has a high scavenging activity.

scholarly journals Antibacterial and Antioxidant Characteristics of Zataria multiflora Boiss Essential Oil and Hydroalcoholic Extract of Rhus coriaria L.

2019 ◽  
Author(s):  
A. Mojaddar Langroodi ◽  
H. Tajik ◽  
T. Mehdizadeh
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Hydroalcoholic Extract ◽  
Zataria Multiflora ◽  
Rhus Coriaria

Background: The increasing demand for natural preservatives results in their extended usefulness. The objective of the present study was to investigate the physicochemical and antioxidative characteristics of Rhus coriaria L. (sumac) fruit and comparison of its antioxidative and antibacterial activity with Zataria multiflora Essential Oil (ZEO) as native Iranian natural additives. Methods: Antioxidant activities of Z. multiflora Boiss and sumac were analyzed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2, 2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS). Reducing power tests were used for measuring antioxidant activity. Total phenolic content of extract and essential oil were studied as well. The Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), and Fractional Inhibitory Concentration (FIC) of a hydroalcoholic extract of sumac and ZEO against of Salmonella Typhimurium and Listeria monocytogenes were studied. Statistical analysis of data was performed using the SPSS software. Results: The phenolic content in sumac extract (305.65 mg/g) was significantly (p˂0.05) higher than ZEO (179.42 mg/100 g). The highest level of antibacterial activity was demonstrated by ZEO with the MICs of 0.625 for S. Typhimurium and 1.25 mg/ml for L. monocytogenes. Conclusion: Sumac extract showed more potent antioxidative activity than ZEO. However, based on the results of antibacterial activity, ZEO had more potent than sumac extract, significantly.

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