scholarly journals Evaluation of vEvaluation of Various Seed Extracts for Their Nematicidal Efficacies Against Root Nematode, Meloidogyne Incognitaarious Seed Extracts for Their Nematicidal Efficacies Against Root Nematode, Meloidogyne Incognita

2021 ◽  
Author(s):  
Zubair Nengroo ◽  
Zeeshan Shah ◽  
Aadil Ganie ◽  
Mohammad Danish
Keyword(s):  
Greenhouse Effect ◽  
Meloidogyne Incognita ◽  
Egg Mass ◽  
Abrus Precatorius ◽  
Positive Effects ◽  
Second Stage ◽  
Bunium Persicum ◽  
Almost All ◽  
Seed Extracts

Abstract Nematicidal activity of seed extracts of eight medicinally important plants viz. Abrus precatorius Linn., Amaranthus virdis Linn., Bunium persicum Boiss., Dioscorea deltoidea Wall. Ex Griseb., Teraxacum officinale Weber., Malva neglecta Wall., Podophylum hexandrum Royle and Robina pseudoacacia Linn. against Meloidogyne incognita was evaluated in vitro at (24, 48 and 72 h) and greenhouse method. After 48 h of exposure, the in vitro results of second stage juveniles (J2s) displayed almost all extracts possess positive effects on J2 mortality. However, dominant mortality was observed by T. officinale 93.67% and B. persicum 89.66% at 72 h exposure. In case of greenhouse effect extracts of T. officinale and B. persicum reduced infestation as compared to inoculated control. The root knot index varied between 1-3 and egg mass index 0-3 as compared to control. This study suggested that seed extracts of these plants can be used for the management of M. incognita and could be used in replacement of synthetic nematicides.

scholarly journals Evaluation of various seed extracts for their nematicidal efficacies against root nematode, Meloidogyne incognita

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Zubair Rehman Nengroo ◽  
Zeshan Umer Shah ◽  
Adil Shafi Ganie ◽  
Mohammad Danish
Keyword(s):  
Meloidogyne Incognita ◽  
Nematicidal Activity ◽  
Egg Mass ◽  
The Novel ◽  
Root Knot Nematode ◽  
Isolation And Purification ◽  
Bunium Persicum ◽  
Bioactive Substance ◽  
Seed Extracts

AbstractThe current study was assumed to identify the novel nematicidal activity of chloroform and methanol (50:50, v/v) seed extracts of eight medicinally important plants viz. Abrus precatorius Linn., Amaranthus virdis Linn., Bunium persicum Boiss., Dioscorea deltoidea Wall. Ex Griseb., Teraxacum officinale Weber., Malva neglecta Wall., Podophylum hexandrum Royle and Robina pseudoacacia Linn. using the root-knot nematode Meloidogyne incognita in in vitro and greenhouse experiments. At 72 h exposure, the extracts were found to be highly nematostatic, where nematodes were completely paralyzed at 12 and 48 h of exposure. However, dominant mortality was observed by T. officinale 93.67% and B. persicum 89.66% seed extracts at 72 h. In greenhouse method, extracts of T. officinale and B. persicum extracts were found to be most potent in reducing number of galls (1.76 and 2.37) and number of egg masses (0.06 and 2.18) respectively as compared to inoculated control. The root knot index of all extracts varied between 1–3 and egg mass index 0–3 as compared to control. This study suggested that seed extracts of these plants can be used for the management of M. incognita and could be used in replacement of synthetic nematicides upon further isolation and purification of bioactive substance responsible for nematicidal activity.

scholarly journals Evaluation of some plant extracts for nemato-toxic potential against juveniles of Meloidogyne incognita in vitro

2018 ◽  
Vol 7 (2) ◽  
pp. 141-145
Author(s):  
Gulwaiz Akhter ◽  
◽  
Tabreiz Ahmad Khan ◽  
Keyword(s):  
Meloidogyne Incognita ◽  
Xanthium Strumarium ◽  
Plant Leaves ◽  
Leaf Extracts ◽  
Argemone Mexicana ◽  
Juvenile Mortality ◽  
Second Stage ◽  
Stage Juvenile ◽  
Toxic Potential

Aqueous leaf extracts were utilized to assess the nematicidal or nematostatic property on second stage juvenile of Meloidogyne incognita. The juvenile were incubate at various concentration of leaf extract viz., 250, 500, 1000 and 2000 ppm. Corrected mortality using Abbot’s formula was recorded after 12, 24 and 48 hours respectively. Correlation coefficient (Pearson) was checked to explain the association between percentages mortality of juvenile with extract concentrations. Linear regression was used to denote concentration and rank dependent outcome of four aqueous plant leaves extracts on the second stage juvenile (J2) mortality. All leaf extracts were found to be nematicidal or nematostatic in property. Maximum juvenile mortality rate was recorded in Xanthium strumarium throughout the incubation period as followed by Acalypha indica, Argemone mexicana and Colocasia gigantean. Concentration depended effect of X. strumarium and C. gigantean proved maximum and minimum level when analyzed by values of regression and correlation. Aqueous leaves extracts of these aforementioned weeds give us an idea about nematicidal properties and therefore may be used as biopesticide in future

Toxicity of ethanol solutions and vapours against Meloidogyne incognita

Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 271-280 ◽  
Author(s):  
Julio C.P. Silva ◽  
Vicente P. Campos ◽  
Eduardo S. Freire ◽  
Willian C. Terra ◽  
Liliana E. Lopez
Keyword(s):  
Root System ◽  
Meloidogyne Incognita ◽  
Parasitic Nematodes ◽  
Infested Soil ◽  
Large Reduction ◽  
Plant Parasitic Nematodes ◽  
Second Stage ◽  
Plant Parasitic

Ethanol (EtOH) is less harmful to humans than currently available nematicide molecules. This study evaluated the efficacy of EtOH in controlling Meloidogyne incognita in vitro and in lettuce plants under glasshouse conditions. Aqueous EtOH solutions (5-70% volume) and their vapours caused an acute nematicidal effect in vitro in second-stage juveniles (J2) of M. incognita and reduced hatching of J2. There was a large reduction of galls and eggs in the root system when 40 ml of EtOH was applied to M. incognita-infested soil at concentrations of 40 and 70%. Water exposed to EtOH vapours for 1 h became toxic, and a 12-h exposure caused 100% J2 mortality. Use of a plastic cover did not increase the efficiency of EtOH in controlling M. incognita in lettuce plants. The observed EtOH effects indicate its prospective use in controlling plant-parasitic nematodes, especially in glasshouses.

scholarly journals Investigating the in vitro and in vivo nematicidal performance of structurally related macrolides against the root-knot nematode, Meloidogyne incognita

2019 ◽  
Vol 12 (1) ◽  
pp. 24-37
Author(s):  
M.A. Radwan ◽  
A.S.A. Saad ◽  
H.A. Mesbah ◽  
H.S. Ibrahim ◽  
M.S. Khalil
Keyword(s):  
Meloidogyne Incognita ◽  
Root Length ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Egg Hatching ◽  
Shoot Height ◽  
Egg Masses ◽  
New Family

Summary Avermectins and spinosyns are structurally related natural products of microbial origin and belong to a new family of macrolides which are active against a vast array of invertebrate pests. In the present study, the effects of four members of macrolides; abamectin (ABM), emamectin benzoate (EMB), spinosad (SPI) and spinetoram (SPIT), on Meloidogyne incognita were investigated under in vitro and in vivo conditions. All compounds reduced egg hatching and led to high mortality of the nematode second-stage juveniles (J2). ABM showed the maximum rate of egg hatching inhibition and J2 mortality while SPIT recorded the minimum. All treatments reduced the number of galls, egg masses, eggs/egg mass in roots and J2 in the soil when compared to the control. Based on the 10 folds of the 24 h-LC50 values of J2 mortality in vitro, EMB and ABM exhibited higher percent reduction in galls (79.68 and 71.45%), egg masses (75.19 and 70.54%), eggs/egg mass (60.49 and 40.91%) and J2 in the soil (90.31 and 86.54%), respectively, compared to SPI and SPIT. Significant increase in tomato shoot height occurred in all biopesticides (10 folds) and SPIT (20 folds). SPI at 10 folds of the 24 h-LC50 values of J2 mortality in vitro, significantly increased root length while ABM at 50 folds and SPIT at 20 folds decreased root length by 5.15% and 5.88%, respectively, compared to the untreated inoculated plants. In all treatments, the dry shoot and root weights increased, compared to the untreated control. Our findings suggest that these macrolides have the ability to regulate nematode population densities and may be an alternative to classical nematicides.

scholarly journals Nematicidal activity of a biopolymer chitosan at different molecular weights against root-knot nematode, Meloidogyne incognita

2012 ◽  
Vol 48 (No. 4) ◽  
pp. 170-178 ◽  
Author(s):  
M.S. Khalil ◽  
M.E.I. Badawy
Keyword(s):  
Molecular Weight ◽  
Meloidogyne Incognita ◽  
Nematicidal Activity ◽  
Egg Mass ◽  
Low Molecular Weight ◽  
Root Knot Nematode ◽  
Molecular Weights ◽  
Tomato Seedlings ◽  
Low Molecular Weight Chitosan

The nematicidal activity of four molecular weights (2.27 &times; 10<sup>5</sup>, 3.60 &times; 10<sup>5</sup>, 5.97 &times; 10<sup>5</sup>, and 9.47 &times; 10<sup>5</sup> g/mol) of a biopolymer chitosan was assayed against the root-knot nematode, Meloidogyne incognita, in vitro and in pot experiments. In laboratory assays, the nematode mortality was significantly influenced by exposure times and chitosan molecular weight. Low molecular weight chitosan (2.27 &times; 10<sup>5</sup> g/mol) was the most effective in killing the nematode with EC<sub>50</sub> of 283.47 and 124.90 mg/l after 24 and 48 h of treatment, respectively. In a greenhouse bioassay, all the compounds mixed in soil at one- and five-fold concentrations of the LC<sub>50</sub> value significantly reduced population, egg mass, and root galling of tomato seedlings compared with the untreated control. In general, the nematicidal activity of these compounds was increased dramatically with a decrease in the molecular weight. The results suggest that the chitosan at low molecular weight may serve as a natural nematicide

In vitro evaluation of some bacterial isolates as biofertilizers and biocontrol agents against the second stage juveniles of Meloidogyne incognita

2010 ◽  
Vol 26 (12) ◽  
pp. 2249-2256 ◽  
Author(s):  
Muhammed E. El-Hadad ◽  
Magdi I. Mustafa ◽  
Shawky M. Selim ◽  
Ahmed E. A. Mahgoob ◽  
Tarek S. El-Tayeb ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Biocontrol Agents ◽  
Bacterial Isolates ◽  
In Vitro Evaluation ◽  
Second Stage

Nematostatic activity of aqueous extracts of West African Crotalaria species

Nematology ◽  
2004 ◽  
Vol 6 (5) ◽  
pp. 765-771 ◽  
Author(s):  
Philippe Jourand ◽  
Thierry Mateille ◽  
Mireille Fargette ◽  
Sylvie Rapior
Keyword(s):  
Multivariate Analysis ◽  
Meloidogyne Incognita ◽  
Plant Tissue ◽  
Green Manure ◽  
West African ◽  
Aqueous Extracts ◽  
Root Extracts ◽  
Second Stage ◽  
Meloidogyne Species

AbstractThe in vitro paralysis of second-stage juveniles of Meloidogyne incognita, M. javanica and M. mayaguensis by aqueous extracts of 15 West African Crotalaria species was analysed. A multivariate analysis distinguished four groups, based on their nematostatic activity: i) C. glaucoides extracts were not active; ii) the root extracts from C. goreensis, C. lathyroides and C. perrottetii were more active than the shoot extracts; iii) the shoot extracts from C. comosa and C. cylindrocarpa were more active than the root extracts; iv) both shoot and root extracts of C. atrorubens, C. barkae, C. grantiana, C. hyssopifolia, C. pallida, C. podocarpa, C. retusa, C. senegalensis and C. sphaerocarpa have nematostatic effects depending on either the Crotalaria species or plant tissue from which the extracts were derived. However, considering the greater biomass contribution of the leaves and stems compared to the roots when the plants are used as green manure, C. barkae, C. grantiana, C. pallida and C. podocarpa are the most efficient Crotalaria species, whatever the Meloidogyne species targeted.

Sign in / Sign up

Export Citation Format

Share Document