scholarly journals Transient Silence of VvCSN5 Enhances Powdery Mildew Resistance in Grapevine (Vitis Vinifera)

2021 ◽  
Author(s):  
Kai-Cheng Cui ◽  
Min Liu ◽  
Gui-Hua Ke ◽  
Xing-Yuan Zhang ◽  
Bo Mu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Expression Patterns ◽  
Marker Genes ◽  
Thompson Seedless ◽  
Basal Expression ◽  
Significant Yield ◽  
Powdery Mildew Pathogen ◽  
Cell Wall Deposition ◽  
Vitis Pseudoreticulata

Abstract As one of the most economically important fruit crops in the world, grapevine (Vitis vinifera) suffers significant yield losses from many pathogens including powdery mildew caused by Erysiphe necator. By contrast, several wild Chinese grapevines including Vitis pseudoreticulata accession ‘Baihe-35-1’ exhibit a high resistance to powdery mildew pathogen. Here, we identified a grapevine gene CSN5 (COP9 signalosome complex subunit 5), designated VvCSN5, which showed different expression patterns in ‘Baihe-35-1’ and in susceptible cultivar V. vinifera ‘Thompson Seedless’ during powdery mildew isolate En NAFU1 infection. Moreover, transient silence of VvCSN5 in ‘Thompson Seedless’ leaves enhanced resistance to En NAFU1, which is accompanied by cell wall deposition at the attempt sites, and hypersensitive response-like cell death of penetrated epidermal cells. Several defense-related marker genes (VvPR1, VvPR3, VvPAD4, and VvRBOHD) had higher basal expression levels in VvCSN5-silenced leaves. In addition, we found the structure and activity of CSN5 promoters in ‘Thompson Seedless’ and ‘Baihe-35-1’ were discrepant, which may be one of the reasons for their different resistance to powdery mildew infection. Taken together, these results imply that grapevine CSN5 plays an important role in the responses to powdery mildew.

scholarly journals Identification of grapevine MLO gene candidates involved in susceptibility to powdery mildew

2008 ◽  
Vol 35 (12) ◽  
pp. 1255 ◽  
Author(s):  
Angela Feechan ◽  
Angelica M. Jermakow ◽  
Laurent Torregrosa ◽  
Ralph Panstruga ◽  
Ian B. Dry
Keyword(s):  
Phylogenetic Analysis ◽  
Powdery Mildew ◽  
Gene Family ◽  
Abiotic Factors ◽  
Expression Patterns ◽  
Tissue Expression ◽  
Vitis Vinifera L ◽  
Cdna Sequences ◽  
Grape Genome ◽  
Powdery Mildew Pathogen

The European cultivated grapevine, Vitis vinifera L., is a host for the powdery mildew pathogen Erisyphe necator, which is the most economically important fungal disease of viticulture. MLO proteins mediate powdery mildew susceptibility in the model plant species Arabidopsis and the crop plants barley and tomato. Seven VvMLO cDNA sequences were isolated from grapevine and were subsequently identified as part of a 17 member VvMLO gene family within the V. vinifera genome. Phylogenetic analysis of the 17 VvMLO genes in the grape genome indicated that the proteins they encode fall into six distinct clades. The expression of representative VvMLOs from each clade were analysed in a range of grape tissues, as well as in response to a range of biotic and abiotic factors. The VvMLOs investigated have unique, but overlapping tissue expression patterns. Expression analysis of VvMLO genes following E. necator infection identified four upregulated VvMLOs which are orthologous to the Arabidopsis AtMLO2, AtMLO6 and AtMLO12 and tomato SlMLO1 genes required for powdery mildew susceptibility. This suggests a degree of functional redundancy between the proteins encoded by these genes in terms of susceptibility to powdery mildew, and, as such, represent potential targets for modification to generate powdery mildew resistant grapevines.

scholarly journals Expression Pattern Analysis of Four Mlo Genes from Rose

2015 ◽  
Vol 140 (4) ◽  
pp. 333-338 ◽  
Author(s):  
Xianqin Qiu ◽  
Hongying Jian ◽  
Qigang Wang ◽  
Kaixue Tang ◽  
Manzhu Bao
Keyword(s):  
Powdery Mildew ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Early Time ◽  
Resistance Locus ◽  
Loss Of Function ◽  
Powdery Mildew Fungus ◽  
Mildew Resistance ◽  
Powdery Mildew Pathogen ◽  
External Stimuli

Rose (Rosa hybrida) is one of the most economically important ornamentals worldwide. Powdery mildew (Podosphaera pannosa) is a major disease in cut and potted roses. In dicots such as arabidopsis (Arabidopsis thaliana), pea (Pisum sativum), and tomato (Solanum lycopersicum), loss-of-function mutations in mildew resistance locus o (Mlo) genes confer high levels of broad-spectrum resistance to powdery mildew. Here, we present spatiotemporal expression patterns of four Mlo genes from R. hybrida based on real-time fluorescence quantitative polymerase chain reaction (qPCR). Phylogenetically closely related R. hybrida mildew resistance locus o (RhMLO) genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli. RhMLO1 and RhMLO2 transcriptional levels were upregulated more than 2-fold by external stimuli, especially by inoculation with powdery mildew fungus P. pannosa at early time points. This phenomenon was not found for RhMLO3 or RhMLO4. The results indicated that RhMLO1 and RhMLO2 might play important roles in rose–powdery mildew pathogen interactions. Our findings may provide useful information for the study of mechanisms of powdery mildew susceptibility in rose.

Cloning and Characterization of a Novel Wheat Glycoside Hydrolase Gene TaGlc2 Induced by Powdery Mildew Pathogen (Erysiphe graminis) Infection

2009 ◽  
Vol 35 (5) ◽  
pp. 786-794
Author(s):  
N PUDAKE Ramesh ◽  
Ming-Ming XIN ◽  
Yu-Jing YIN ◽  
Chao-Jie XIE ◽  
Zhong-Fu NI ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Glycoside Hydrolase ◽  
Erysiphe Graminis ◽  
Powdery Mildew Pathogen

Spectral characterization of fungal diseases downy mildew, powdery mildew, black-foot and Petri disease on Vitis vinifera leaves

2021 ◽  
Vol 42 (15) ◽  
pp. 5680-5697
Author(s):  
Pâmela A. Pithan ◽  
Jorge R. Ducati ◽  
Lucas R. Garrido ◽  
Diniz C. Arruda ◽  
Adriane B. Thum ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Vitis Vinifera ◽  
Downy Mildew ◽  
Fungal Diseases ◽  
Spectral Characterization ◽  
Petri Disease ◽  
Black Foot

scholarly journals Transformation of the cucurbit powdery mildew pathogen Podosphaera xanthii by Agrobacterium tumefaciens

2016 ◽  
Vol 213 (4) ◽  
pp. 1961-1973 ◽  
Author(s):  
Jesús Martínez‐Cruz ◽  
Diego Romero ◽  
Antonio Vicente ◽  
Alejandro Pérez‐García
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Powdery Mildew ◽  
Podosphaera Xanthii ◽  
Powdery Mildew Pathogen ◽  
Cucurbit Powdery Mildew

scholarly journals Identification of cell-type-specific marker genes from co-expression patterns in tissue samples

2021 ◽  
Author(s):  
Yixuan Qiu ◽  
Jiebiao Wang ◽  
Jing Lei ◽  
Kathryn Roeder
Keyword(s):  
Single Cell ◽  
Expression Patterns ◽  
R Package ◽  
Supplementary Information ◽  
Marker Genes ◽  
Specific Marker ◽  
Cell Type ◽  
Correlation Pattern ◽  
Tissue Samples ◽  
Bulk Data

Abstract Motivation Marker genes, defined as genes that are expressed primarily in a single cell type, can be identified from the single cell transcriptome; however, such data are not always available for the many uses of marker genes, such as deconvolution of bulk tissue. Marker genes for a cell type, however, are highly correlated in bulk data, because their expression levels depend primarily on the proportion of that cell type in the samples. Therefore, when many tissue samples are analyzed, it is possible to identify these marker genes from the correlation pattern. Results To capitalize on this pattern, we develop a new algorithm to detect marker genes by combining published information about likely marker genes with bulk transcriptome data in the form of a semi-supervised algorithm. The algorithm then exploits the correlation structure of the bulk data to refine the published marker genes by adding or removing genes from the list. Availability and implementation We implement this method as an R package markerpen, hosted on CRAN (https://CRAN.R-project.org/package=markerpen). Supplementary information Supplementary data are available at Bioinformatics online.

Antimicrobial constituents of Thompson seedless raisins (Vitis vinifera) against selected oral pathogens

2008 ◽  
Vol 1 (3) ◽  
pp. 151-154 ◽  
Author(s):  
J. Fausto Rivero-Cruz ◽  
Min Zhu ◽  
A. Douglas Kinghorn ◽  
Christine D. Wu
Keyword(s):  
Vitis Vinifera ◽  
Thompson Seedless ◽  
Oral Pathogens

scholarly journals RING-H2-type E3 gene VpRH2 from Vitis pseudoreticulata improves resistance to powdery mildew by interacting with VpGRP2A

2017 ◽  
Vol 68 (7) ◽  
pp. 1669-1687 ◽  
Author(s):  
Lei Wang ◽  
Xiaoqing Xie ◽  
Wenkong Yao ◽  
Jie Wang ◽  
Fuli Ma ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Vitis Pseudoreticulata
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