scholarly journals In Vitro Antibacterial Activity of Different Honey Samples Against Clinical Isolates

2021 ◽  
Author(s):  
Mahasin Ahmed Wadi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Clinical Isolates ◽  
Base Line ◽  
Drug Resistant ◽  
Low Sensitivity

Abstract BackgroundThe emergence of multi–drug resistant organisms has created a lot of clinicalproblems. Hence there is a need to find natural alternative treatment to counter the multi–drug resistant organisms. Honey has a well-established usage as wound dressing in ancient and traditional medicine. The aim of this work is to establish a base-line of the antibacterial activity of 32 natural and commercial various honey samples against 8 clinical isolates.MethodsThirty two honey samples (raw and commercial honey) collected from different countries with different floral origin were tested in vitro for antibacterial activity against 8 clinical isolates collected from patients, at private Hospital from Sudan, using disk diffusion technique. The following 6 Epsilometer test (E Test), Amoxicillin, Augamentin, Ceftaxime, Chloramphenicol, Gentamicin and tetracycline were used against 8 clinical isolates for Minimum Inhibitory Concentration.ResultsThe following 8 clinical isolates were identified by conventional bacteriological methods; Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aerouginosa, Proteus.vulgaris, Salmonella typhi, Shigella sonnei and Methicillin Resistant Staphylococcus aureus (MRSA). Honey has been shown to have antibacterial properties against both Gram-positive and Gram-negative bacteria. The tested organisms showed low sensitivity to antibiotics E test.ConclusionMost of the bacterial species studied were uniformly receptive to all the tested honey. In contrast The tested organisms showed low sensitivity to antibiotics with low MIC.

scholarly journals Antibacterial activity of Taxodium ascendens Diterpenes against Methicillin-resistant Staphylococcus aureus

2014 ◽  
Vol 9 (8) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Courtney M. Starks ◽  
Vanessa L. Norman ◽  
Russell B. Williams ◽  
Matt G. Goering ◽  
Stephanie M. Rice ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Methicillin Resistant

One new and seven known diterpenes were identified from an antibacterial chromatographic fraction of Taxodium ascendens. Of these, demethylcryptojaponol (2), 6-hydroxysalvinolone (3), hydroxyferruginol (4), and hinokiol (5) demonstrated potent activity against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). These compounds represent a class of synthetically accessible compounds that could be further developed for treatment of drug-resistant bacterial infections.

scholarly journals Antibacterial Activity of Manuka Honey Against Azithromycin Resistant Extensively Drug Resistant Salmonella Typhi Clinical Isolates: In-Vitro Study

2022 ◽  
Author(s):  
Kokab Jabeen ◽  
Sidrah Saleem ◽  
Faiqa Arshad ◽  
Zill-e-Huma ◽  
Shah Jahan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Alternative Treatment ◽  
Therapeutic Potential ◽  
Salmonella Typhi ◽  
Drug Resistant ◽  
Manuka Honey ◽  
Extensively Drug Resistant ◽  
Alternative Treatment Option ◽  
Wide Range

Abstract Typhoid fever is a significant health problem in developing countries like Pakistan. Salmonella Typhi the causative agent of typhoid has developed resistant to almost all recommended antibiotics. Emergence of resistance to third generation cephalosporins has further complicated the situation and such strains are called as extensively drug resistant (XDR) Salmonella Typhi. Currently only available options are azithromycin and cabapenems. Recently few reports of azithromycin resistance have emerged from countries like Pakistan, India, Bangladesh and Nepal. As azithromycin is the only oral option available to treat XDR Typhoid, development of resistance may change treatment strategy altogether from out patient management to hospitalization of every patient. This may increase the burden on already weak health care system of countries like Pakistan. So there is dire need to look for the alternative treatment options. Manuka honey is well known for its therapeutic potential against wide range of bacteria including Salmonella Typhimurium. In this study 3 azithromycin resistant isolates were isolated and identified using disc diffusion, E-test and broth micro dilution methods and antibacterial activity, MIC and MBC of manuka honey was performed by agar well diffusion assay and broth micro dilution assay respectively. Manuka honey manifested significant antibacterial activity against all test isolates with zone of inhibition ranging from 7.3mm to 7.5mm, MIC and MBC values were between 10 to 15% v/v Here, we conclude that Manuka honey possess potent antibacterial activity and might be used as an alternative treatment option against azithromycin resistant XDR Typhid. However, further clinical trials are mandatory to validate our initial findings.

scholarly journals Synthesis of Mixed Ligand Ruthenium (II/III) Complexes and Their Antibacterial Evaluation on Drug-Resistant Bacterial Organisms

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
James T. P. Matshwele ◽  
Florence Nareetsile ◽  
Daphne Mapolelo ◽  
Pearl Matshameko ◽  
Melvin Leteane ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Magnetic Susceptibility ◽  
Klebsiella Pneumoniae ◽  
Bacterial Species ◽  
Mixed Ligand ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Bacterial Dna ◽  
Polypyridyl Ligand

The potential antimicrobial properties of a tridentate polypyridyl ligand 4-butoxy-N,N-bis(pyridin-2-ylmethyl)aniline (BUT) 1 and its corresponding mixed ligand ruthenium complexes were investigated on drug-resistant and non-drug-resistant bacterial species. The ligand and its complexes were synthesized and successfully characterized by 1H NMR, UV/Vis, and FTIR spectra; ESI-MS; and magnetic susceptibility. Electronic spectra and magnetic susceptibility of these Ru(II)/(III) complexes suggest that they are of a low spin crystal field split, where the Ru(III) is a d5 and Ru(II) d6 low spin. These compounds were tested for antibacterial activity on two bacterial species: Staphylococcus aureus (S. aureus) and Klebsiella pneumoniae (K. pneumoniae), as well as their drug-resistant strains methicillin-resistant Staphylococcus aureus (MRSA) and multidrug resistant Klebsiella pneumoniae (MDR K. pneumoniae). All the compounds inhibited growth of the two non-drug-resistant bacteria and only one drug-resistant strain MRSA. However, only the ligands BUT and 2,2-dipyridylamine showed activity against MRSA, while all complexes did not show any antibacterial activity on MRSA. We observed large zones of inhibition for the Gram-positive S. aureus and MRSA bacteria, compared to the Gram-negative K. pneumoniae bacteria. DNA cleavage studies with gel electrophoresis showed denatured bacterial DNA on the gel from all the complexes, with the exception of the ligand, suggesting DNA nuclease activity of the complexes in the bacterial DNA.

scholarly journals Antibacterial Co(II), Ni(II), Cu(II) and Zn(II) complexes with biacetyl-derived Schiff bases

2010 ◽  
Vol 75 (8) ◽  
pp. 1075-1084 ◽  
Author(s):  
Muhammad Imran ◽  
Mitu Liviu ◽  
Shoomaila Latif ◽  
Zaid Mahmood ◽  
Imtiaz Naimat ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Schiff Base ◽  
Metal Complexes ◽  
Schiff Bases ◽  
Bacterial Species ◽  
Electronic Spectroscopy ◽  
Salmonella Typhi ◽  
Schiff Base Ligands ◽  
No Donor

The condensation reactions of biacetyl with orthohydroxyaniline and 2-aminobenzoic acid to form bidendate NO donor Schiff bases were studied. The prepared Schiff base ligands were further utilized for the formation of metal chelates having the general formula [ML2.2H2O] where M = Co(II), Ni(II), Cu(II) and Zn(II) and L = HL1 and HL2. These new compounds were characterized by conductance measurements, magnetic susceptibility measurements, elemental analysis, and IR, 1H-NMR and electronic spectroscopy. Both Schiff base ligands were found to have a mono-anionic bidentate nature and octahedral geometry was assigned to all metal complexes. All the complexes contained coordinated water which was lost at 141-160 ?C. These compounds were also screened for their in-vitro antibacterial activity against four bacterial species, namely; Escherichia coli, Staphylococcus aureus, Salmonella typhi and Bacillus subtillis. The metal complexes were found to have greater antibacterial activity than the uncomplexed Schiff base ligands.

scholarly journals In VitroAntibacterial and Antifungal Activity of Salicylanilide Benzoates

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Martin Krátký ◽  
Jarmila Vinšová ◽  
Vladimír Buchta
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Benzoic Acid ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Phenyl Benzoate ◽  
Methicillin Resistant ◽  
Micromolar Range

The resistance to antimicrobial agents brings a need of novel antimicrobial agents. We have synthesized and found thein vitroantibacterial activity of salicylanilide esters with benzoic acid (2-(phenylcarbamoyl)phenyl benzoates) in micromolar range. They were evaluatedin vitrofor the activity against eight fungal and eight bacterial species. All derivatives showed a significant antibacterial activity against Gram-positive strains with minimum inhibitory concentrations ≥0.98 μmol/L including methicillin-resistantStaphylococcus aureusstrain. The most active compounds were 5-chloro-2-(3,4-dichlorophenylcarbamoyl)phenyl benzoate and 4-chloro-2-(4-(trifluoromethyl)phenylcarbamoyl)phenyl benzoate. The antifungal activity is significantly lower.

scholarly journals Synthesis and Reactions of 3-(2-Chloromethyl-carbonylamino)tetrachloroquinazolin-2,4-dione

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
M. A. Hassan ◽  
A. M. M. Younes ◽  
M. M. Taha ◽  
A. Abdel-Monsef
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Elemental Analysis ◽  
Salmonella Typhi ◽  
Synthetic Route ◽  
H Nmr ◽  
In Vitro Antibacterial Activity

A series of tetrachloroquinazolin-2,4-dione derivatives were synthesized using appropriate synthetic route and characterized by IR, 1H NMR, MS, and elemental analysis. The synthesized compounds were evaluated for their preliminary in vitro antibacterial activity towards Salmonella typhi, Staphylococcus aureus, and Bacillus cereus.

scholarly journals Microwave-Assisted Synthesis, Structural Characterization and Assessment of the Antibacterial Activity of Some New Aminopyridine, Pyrrolidine, Piperidine and Morpholine Acetamides

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 533
Author(s):  
Abdulmajeed S. H. Alsamarrai ◽  
Saba S. Abdulghani
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Molecular Structures ◽  
Secondary Amines ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

A series of new acetamide derivatives 22–28 of primary and secondary amines and para-toluene sulphinate sodium salt have been synthesized under microwave irradiation and assessed in vitro for their antibacterial activity against one Gram-positive and two Gram-negative bacterial species such as S. pyogenes, E. coli, and P. mirabilis using the Mueller-Hinton Agar diffusion (well diffusion) method. The synthesized compounds with significant differences in inhibition diameters and MICs were compared with those of amoxicillin, ampicillin, cephalothin, azithromycin and doxycycline. All of the evaluated acetamide derivatives were used with varying inhibition concentrations of 6.25, 12.5, 37.5, 62.5, 87.5, 112.5 and 125 µg/mL. The results show that the most important antibacterial properties were displayed by the synthetic compounds 22 and 24, both of bear a para-chlorophenyl moiety incorporated into the 2-position moiety of acetamide 1. The molecular structures of the new compounds were determined using the FT-IR and 1H-NMR techniques.

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