scholarly journals An Iron-Free VMP35 Multi-nutrient Complex Modulates an Array of Homeostatic Biological Parameters: Promoting Hemoglobinization, Aerobic Metabolism, Viral Immuno-competence, and Inflammatory Regulation

2020 ◽  
Author(s):  
Kenneth Blum ◽  
Berrnard W Downs ◽  
MANASHI BAGCHI ◽  
Steve W Kushner ◽  
Bruce S. Morrison ◽  
...  
Keyword(s):  
Systems Biology ◽  
Case Studies ◽  
Inflammatory Responses ◽  
Human Study ◽  
Aerobic Metabolism ◽  
University Hospital ◽  
Deficiency Anemia ◽  
Immune Competence ◽  
Biological Parameters ◽  
Inflammatory Regulation

Abstract Background: Integrity of hemoglobin is a crucial determining factor for the overall health functions. Nutrient repletion therapy should be a fundamental strategy to restore the healthy properties of blood. A unique patent-pending iron-free VMP35 formulation was engineered by our laboratory to restore iron-dependent hemoglobin in anemic cells using a proprietary Prodosome® absorption technology. This formulation, containing an array of nano-emulsified botanical ingredients rich in bioflavonoids, strengthens the structural integrity of connective tissues, and potentiates immune competence, cellular aerobic metabolism, and enhances efficient regulation of inflammatory events. We discuss the intricate aspects of strong vs. fragile immunity and consequential inflammatory responses to convey a deeper understanding of the varied and overly complex sequela of immunological behaviors and events. The effect of the VMP35 is mediated through highly absorbable nutritional/nutrigenomic repletion enabling improvements in the systemic set of functional behaviors. In fact, the iron-free VMP35 facilitates a “Systems Biology Approach” which restores hemoglobin status, reverses anaerobic hypoxia, improves competent immune responsivity, and regulates appropriate and controlled activation of inflammatory sequela. Under these pathogenic circumstances, iron-deficiency anemia has been misconceptualized, and a new nosological term, Chronic Anemia Syndrome, is proposed. The comparative therapeutic rationale of Reductionist vs. Systems Biology approaches is also explained in detail. Methods: The efficacy of the novel therapeutic iron-free VMP35 liquid nutraceutical is detailed in restoring iron-dependent hemoglobin to RBCs and boosting cellular morphology, viability, and immune competence, thereby reducing the need for prolonging inflammatory sequela. Results: This was demonstrated in a previous IRB approved multi-subject human study. In addition, two recent case studies report dramatic restorative benefits of nutrient repletion therapy of the VMP35 on subjects having experienced near-fatal events, which confirmed the findings explained in this manuscript. Conclusions: This novel iron-free VMP35 modulates an array of homeostatic biological parameters such as enhanced hemoglobinization, aerobic metabolism, viral immuno-competence, and inflammatory regulation. Further research, examining mechanistic and beneficial effects in athletic performance, is in progress. Importantly, during these troubled immune challenging times, modulating an array of homeostatic immunological and inflammatory dysfunctions are tantamount to improved population outcomes. Trial Registration: The Clinical investigation in a total of 38 subjects was conducted under an Institutional Review Board (IRB) from the Path Foundation in New York, NY (#13-009 April 25, 2013). The two case studies were done at Lancaster General Hospital, Lancaster, PA, and Jefferson University Hospital, Philadelphia, PA, USA. Both studies were retrospectively registered.

scholarly journals Integrative Systems Biology Approaches to Identify Potential Biomarkers and Pathways of Cervical Cancer

2021 ◽  
Vol 11 (5) ◽  
pp. 363
Author(s):  
Arafat Rahman Oany ◽  
Mamun Mia ◽  
Tahmina Pervin ◽  
Salem Ali Alyami ◽  
Mohammad Ali Moni
Keyword(s):  
Cervical Cancer ◽  
Systems Biology ◽  
Differentially Expressed Genes ◽  
Inflammatory Responses ◽  
Profile Analysis ◽  
Target Identification ◽  
Regulatory Genes ◽  
Differentially Expressed ◽  
Hub Genes ◽  
Potential Biomarkers

Nowadays, cervical cancer (CC) is treated as the leading cancer among women throughout the world. Despite effective vaccination and improved surgery and treatment, CC retains its fatality rate of about half of the infected population globally. The major screening biomarkers and therapeutic target identification have now become a global concern. In the present study, we have employed systems biology approaches to retrieve the potential biomarkers and pathways from transcriptomic profiling. Initially, we have identified 76 of each up-regulated and down-regulated gene from a total of 4643 differentially expressed genes. The up-regulatory genes mainly concentrate on immune-inflammatory responses, and the down-regulatory genes are on receptor binding and gamma-glutamyltransferase. The involved pathways associated with these genes were also assessed through pathway enrichment, and we mainly focused on different cancer pathways, immunoresponse, and cell cycle pathways. After the subsequent enrichment of these genes, we have identified 12 hub genes, which play a crucial role in CC and are verified by expression profile analysis. From our study, we have found that genes LILRB2 and CYBB play crucial roles in CC, as reported here for the first time. Furthermore, the survivability of the hub genes was also assessed, and among them, finally, CXCR4 has been identified as one of the most potential differentially expressed genes that might play a vital role in the survival of CC patients. Thus, CXCR4 could be used as a prognostic and/or diagnostic biomarker and a drug target for CC.

scholarly journals Justification of Universal Iron Supplementation for Infants 6-12 months in Regions with a High Prevalence of Thalassemia

2021 ◽  
Author(s):  
Phakatip Sinlapamongkolkul ◽  
Pacharapan Surapolchai ◽  
Vip Viprakasit
Keyword(s):  
Iron Deficiency ◽  
Iron Supplementation ◽  
Complete Blood Count ◽  
Iron Status ◽  
Transferrin Saturation ◽  
University Hospital ◽  
Deficiency Anemia ◽  
Increased Risk ◽  
Thalassemia Minor ◽  
Hb E

Abstract Background Many clinicians hesitate adopting a universal infant iron supplementation program due to the risk of increased iron absorption for those with thalassemia. We aimed to determine thalassemia prevalence in 6- to 12-month old infants, along with the iron status of those with and without thalassemia. Procedures: We performed a cross-sectional descriptive study of infants attending the Well Baby Clinic at Thammasat University Hospital for routine checkups. Complete blood count, hemoglobin electrophoresis, iron parameters, and molecular genetics for common α- and β-thalassemia were evaluated. Results Overall, 97 of 206 (47%) participants had thalassemia minor, the majority having Hb E traits. None had thalassemia intermedia or major. Familial history of anemia or thalassemia presented an increased risk of detecting thalassemia minor in offspring (OR 5.18; 95% CI 2.60-10.33, p = 0.001). There were no statistical differences in transferrin saturation, serum ferritin and hepcidin between iron-replete infants with thalassemia minor and those without. However, one-third of infants with thalassemia minor (31/97) also had iron deficiency anemia (IDA), with a similar risk of having iron deficiency to infants without thalassemia. There was no hepcidin suppression in our infants with thalassemia minor as compared to controls. Conclusions Both thalassemia and IDA are endemic to Southeast Asia. Infants with thalassemia minor, particularly with Hb E and α-thalassemia traits, are at risk of IDA. Our short-term universal iron supplementation program for 6 to 12-month old infants does not appear to increase the risk of those with thalassemia minor developing iron overload in the future.

scholarly journals Non-steroidal anti-inflammatory drug-induced enteropathy as a major risk factor for small bowel bleeding

2020 ◽  
Author(s):  
Doo-Ho Lim ◽  
Kyoungwon Jung ◽  
Seung Bum Lee ◽  
In Kyu Park ◽  
Hee Jeong Cha ◽  
...  
Keyword(s):  
Small Bowel ◽  
Capsule Endoscopy ◽  
Diagnostic Yield ◽  
University Hospital ◽  
Deficiency Anemia ◽  
Gi Bleeding ◽  
Inflammatory Drug ◽  
Korean Patients ◽  
Common Etiology ◽  
Anti Inflammatory

Abstract Background: Small bowel (SB) bleeding accounts for 5% of all gastrointestinal (GI) bleeding cases and 80% of obscure GI bleeding cases. Although angioectasia is the common etiology of SB bleeding, nonsteroidal anti-inflammatory drug (NSAID)-induced SB lesions are also reported as a major cause in studies from Eastern countries. Herein, we assessed the frequency of occurrence of NSAID-induced SB lesions in Korean patients with obscure GI bleeding.Methods: We retrospectively analyzed medical records of all consecutive patients aged ≥18 years that underwent capsule endoscopy from March 2018 to February 2019 at Ulsan University Hospital and Kosin University Gospel Hospital.Results: Of the 83 subjects (all were Korean; mean age ± standard deviation: 59 ± 18 years; age range: 18–84 years; men: n=52; women: n=31), 55 (66.2%) had stools with clear blood and 28 (33.8%) had normal stools with iron deficiency anemia . The detection rate of SB bleeding and lesions using capsule endoscopy was 72.3% (60 of 83 patients). A significantly higher frequency (40 of 51) of ulcerative/erosive lesions than other causes was observed in patients with inactive bleeding but visible SB lesions. As a result, NSAID-induced enteropathy accounted for 41.7% (25 of 60) of all SB bleeding cases.Conclusions: Contrary to what is reported for patients in Western countries, this study in Korean patients showed an improved diagnostic yield of capsule endoscopy for obscure GI bleeding and that NSAID-induced enteropathy was the most common etiology of SB bleeding. Aggressive small intestine examination is required for patients with unexplained GI bleeding.

scholarly journals An Algorithm for Initial Fluxes of Metabolic P Systems

2009 ◽  
Vol 4 (3) ◽  
pp. 263 ◽  
Author(s):  
Roberto Pagliarini ◽  
Giuditta Franco ◽  
Vincenzo Manca
Keyword(s):  
Experimental Data ◽  
Systems Biology ◽  
System Dynamics ◽  
Case Studies ◽  
Heuristic Algorithm ◽  
Central Issue ◽  
P Systems ◽  
Algebraic Systems ◽  
Temporal Series

A central issue in systems biology is the study of efficient methods inferring fluxes of biological reactions by starting from experimental data. Among the different techniques proposed in the last years, the theory of Metabolic P systems, which is based on the Log-Gain principle, proved to be helpful for deducing biologi- cal fluxes from temporal series of observed dynamics. According to this approach, the algebraic systems provided by the Log-Gain principle determine the reaction fluxes underlying a system dynamics when initial fluxes are known. Here we propose a heuristic algorithm for estimating the initial fluxes, that is tested in two case studies.

scholarly journals Metabolomics-Based Systems Biology Needs Chemometrics – Former Eexperience and Case Studies

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Carmen SOCACIU ◽  
Florinela FETEA ◽  
Floricuţa RANGA ◽  
Raluca POP ◽  
Simona ZAVOI
Keyword(s):  
Systems Biology ◽  
Case Studies ◽  
Biochemical Markers ◽  
Metabolic Profile ◽  
Added Value ◽  
Infrared Spectrometry ◽  
Black Currant ◽  
Vis Spectroscopy ◽  
Data Calibration

Considering the need of symbiosis between metabolomics& chemometrics in developing systems biology, we review here some relevant findings of our previous experience in performing analytical methods for metabolomic fingerprinting of fruits and food products, coupled with chemometrics as an integrated , added-value technology for systems biology. Some specific case-studies relevant for plant and food metabolomics are presented: seabuckthorn fruits and leaves, lipophilic and hydrophilic extracts or juices, aronia, black currant and bilberries. We proposed specific metabolomic-metabonomic evaluations (fingerprint and quantification) integrated by a four-steps analysis: UV-VIS spectroscopy (1), Infrared spectrometry (2), GC or HPLC ± FID, PDA or MS detection (3) and chemometry (4). By our specific case-studies we demonstrated here that the determination of biochemical markers for a specific plant or food is just a beginning of a metabolic approach. Only combining the chemical informations given by sophisticated or rapid, simple techniques associated with chemometrics’ informations via pattern recognition (fingerprinting), data calibration and quantitative measuring, as well clustering of significant groups of samples based on their principal components are necessary to enrich the metabolic profile and approach the intergrated view of systems biology.

scholarly journals Evaluate the frequency of Autoimmune Cutaneous Disorders in patients with Celiac disease.

2019 ◽  
Vol 26 (12) ◽  
pp. 2105-2111
Author(s):  
Quratulain ◽  
Muhammad Aslam Rind ◽  
Maria Nazir ◽  
Muhammad Saleem Rind
Keyword(s):  
Celiac Disease ◽  
Alopecia Areata ◽  
Rural Areas ◽  
Serological Test ◽  
University Hospital ◽  
Deficiency Anemia ◽  
P Value ◽  
Pituitary Insufficiency ◽  
Cutaneous Manifestations ◽  
The Mean

Objectives: To evaluate the frequency of autoimmune cutaneous disorders in patients with celiac disease. Study Design: This descriptive cross sectional study. Setting: conducted at Dermatological Department of Liaquat University Hospital Hyderabad/ Jamshoro. Period: From 1st March 2015 to 31st August 2015. Material & Methods: Total 352 patients of age 15 to 45 years & either gender having celiac disease were enrolled consecutively. Patients with history of abnormal serological test results and already on gluten free diet, known cases of diabetes mellitus, tuberculosis, systemic lupus erythematosus (SLE), secondary syphilis, hyper or hypothyroidism, iron deficiency anemia, pernicious anemia, Addison disease and pituitary insufficiency were excluded. The data was analyzed using SPSS version 16. The frequency/ percentage were calculated for autoimmune cutaneous disorders i-e; Dermatitis herpetiformis, Alopecia areata, vitiligo & psoriasis & for gender. The mean and standard deviation (SD) were calculated for age, duration of celiac disease and duration of cutaneous disorders. Stratified analysis was done followed by applying chi-square with a p value <0.05 taken as significant. Results: The mean age was 29.83 ± 8.91 years (Range 15-45). The mean duration of celiac disease was 5.99 ± 2.30 years (Range: 1-10 years). The Mean duration of cutaneous manifestation was 7.55 ± 3.30 months (Range: 3 to 15). Slight less than half of all patients were females 46.3%. More than two third patients i-e; 69% (n= 243) in this study were from rural areas. About 10.2% patients had dermatitis herpatiformis, 6.3% patients had alopecia areata, 2.6% patients had Vitiligo while 16.5% had psoriasis. The gender, age of patients, duration of CD & duration of development of skin symptoms were non-significant effect modifiers for the frequency of cutaneous manifestations among CD patients. Conclusions: Therefore, it concludes that psoriasis, dermatitis herpatiformis & alopecia areata are quite prevalent in CD patients. Vitiligo is rare condition. Screening for such autoimmune cutaneous manifestations and proper treatment of all such patients should be carried out routinely. 

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