scholarly journals A Novel Imprinted Gene (Sp-Pol) with Sex-specific SNP Locus and Sex-biased Expression Pattern Provides Insights into the Gonad Development of Mud Crab (Scylla Paramamosain)

2021 ◽  
Author(s):  
Ardavan Farhadi ◽  
Shi Xi ◽  
Yin Zhang ◽  
Huaiping Zheng ◽  
Shengkang Li ◽  
...  
Keyword(s):  
Gonad Development ◽  
Expression Level ◽  
Scylla Paramamosain ◽  
Seminiferous Tubules ◽  
Mud Crab ◽  
Eyestalk Ablation ◽  
Imprinted Gene ◽  
New Gene ◽  
Specific Locus ◽  
Female Specific

Abstract Background: We previously discovered a potential sex-specific single nucleotide polymorphism (SNP) locus named SNP1888 in mud crab (Scylla paramamosain). Methods: In this study, we first verified SNP1888 to be truly a female-specific locus and then we identified a novel imprinted gene (designated as Sp-Pol) at the upstream of SNP1888 (SNP1888 is located at the 3’-UTR of Sp-Pol). Moreover, SNP1888 together with Sp-Pol were mapped on LG32 of a high-density genetic map. Results: Phylogenetic analysis showed that Sp-Pol may need to be classified as a new gene family due to the very low sequence identity with other known genes. Sp-Pol was expressed at a higher level in gonads compared to other tissues and its expression level in the testis was much higher than in the ovary. Coincidentally, mono-allelic expression was observed in the ovary. Moreover, Sp-Pol exhibited sex-biased expression with approximately 3- to 4-fold higher in males than in females at fifth (C5) and sixth (C6) crablet stages. During the zoeae development, Sp-Pol had the highest expression at the zoea I stage. After unilateral eyestalk ablation, the expression level of Sp-Pol significantly increased in testis and hepatopancreas in males, while it was downregulated in the hepatopancreas of females. Fluorescence in situ hybridization (FISH) assay revealed that Sp-Pol transcripts were strongly localized in the epithelia of seminiferous tubules of the testis and in the ovary it was detected in the oogonium cells.Conclusion: These results demonstrated that Sp-Pol may play important roles in the sexual development of S. paramamosain.

scholarly journals A Novel Imprinted Gene (Sp-Pol) With Sex-Specific SNP Locus and Sex-Biased Expression Pattern Provides Insights Into the Gonad Development of Mud Crab (Scylla paramamosain)

2021 ◽  
Vol 8 ◽  
Author(s):  
Ardavan Farhadi ◽  
Xi Shi ◽  
Yin Zhang ◽  
Yueling Zhang ◽  
Shengkang Li ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Gonad Development ◽  
Expression Level ◽  
Scylla Paramamosain ◽  
Seminiferous Tubules ◽  
Regulation Mechanism ◽  
Eyestalk Ablation ◽  
Reading Frame ◽  
New Gene

Identification and exploring the role of novel sex-related genes is a crucial step for understanding the regulation mechanism of sexual development. In the present study, we identified a novel sex-related gene (designated as Sp-Pol) upstream of a sex-specific single nucleotide polymorphism (SNP). SNP1888 together with Sp-Pol were mapped on LG32 (which is a sex-related linkage group) of a high-density genetic map. The full-length cDNA of Sp-Pol consists of 1703 bp with an open reading frame (ORF) encoding 204 amino acids, a 344 bp 5′-UTR and a 744 bp 3′-UTR. Phylogenetic analysis showed that Sp-Pol may need to be classified as a new gene family due to the very low sequence identity with other known genes (less than 25% identity). The sex-biased expression pattern of Sp-Pol starts from crablet stage V (C5) with approximately three- to four-fold higher in males than in females. Sp-Pol was expressed at a higher level in gonads compared to other tissues, with the highest expression level in the testis. In testis, a downward trend was observed in the expression level of Sp-Pol from the testis stage I (T1) to testis stage III (T3). After unilateral eyestalk ablation, the expression level of Sp-Pol significantly increased in testis and hepatopancreas in males, while it was downregulated in the hepatopancreas of females. Fluorescence in situ hybridization (FISH) assay revealed that Sp-Pol transcripts were strongly localized in the epithelia of seminiferous tubules of the testis, and in the ovary, it was detected in the oogonium cells. These findings showed that Sp-Pol may play crucial roles in the gonad development of S. paramamosain.

scholarly journals Gonad development and size at maturity of the male mud crab Scylla paramamosain (Forsskål, 1755) in a tropical mangrove swamp

2013 ◽  
Vol 1 (1) ◽  
pp. 7 ◽  
Author(s):  
Md. Sherazul Islam ◽  
Hisashi Kurokura
Keyword(s):  
Sexual Maturity ◽  
Carapace Width ◽  
Gonad Development ◽  
Reproductive Traits ◽  
Development Stage ◽  
Probit Analysis ◽  
Mature Stage ◽  
Scylla Paramamosain ◽  
Sample Population ◽  
Mud Crab

The reproductive traits and size at sexual maturity of the male mud crab Scylla paramamosain were investigated in Pak Phanang mangrove swamps, Thailand. Samples were taken seven times from the local middlemen mud crab traders during June 2006 to January 2008. Gonad development was determined based on histological appearance that was classified into three stages: 1) Immature (Spermatogonia), 2) Maturing (Spermatocytes) and 3) Mature (Spermatids and Spermatozoa). Among the sample population, the highest 72% was under gonad development stage I, whereas mature stage III was only 12%. The size at first maturity was estimated by the external allometric growth and histological observation of gonad. The size at which 50% of individuals attain sexual maturity was estimated by the two mathematical models such as probit analysis and logistic curve. The mean size at first sexual maturity and 50% maturation of male S. paramamosain were 96 mm and 109 mm internal carapace width (ICW) which revealed that 88% individuals were immature. The present result suggested that the minimum legal size of male S. paramamosain capture should be >110 mm ICW.

Identification and characterization of apoptosis regulator Bax involved in air-exposure stress of the mud crab, Scylla Paramamosain Estampador, 1949

Crustaceana ◽  
2017 ◽  
Vol 90 (11-12) ◽  
pp. 1373-1390 ◽  
Author(s):  
Wen-Bin Gu ◽  
Yi-Lian Zhou ◽  
Dan-Dan Tu ◽  
Zhong-Kai Zhou ◽  
Qi-Hui Zhu ◽  
...  
Keyword(s):  
Cell Apoptosis ◽  
Three Dimensional ◽  
Expression Level ◽  
Dimensional Structure ◽  
Scylla Paramamosain ◽  
Control Group ◽  
Mud Crab ◽  
Coding Region ◽  
Air Exposure ◽  
Significant Difference

The mud crab, Scylla paramamosain Estampador, 1949, is a widely farmed commercial species in the South-East coastal areas of China. The crabs are captured and placed in a water-free container for further transportation. The long-time air exposure makes the crabs suffer from oxidative stress, triggering cell apoptosis and leading to the crab’s death. In this study, the homologue of the apoptosis regulator BAX was firstly identified in S. paramamosain and named as SpBAX. The coding region of SpBAX yielded a polypeptide of 278 amino acids, consisting of the defining motif of the BAXs family including Bcl-2 homologous BH1, BH2, BH3 regions and a transmembrane (TM) domain. Subcellular prediction suggested that SpBAX was located in the cytoplasm. The three-dimensional structure showed that SpBAX contained 8 helical regions. Two central α-helices (α5 and α6) flanked on one side by α3 and α4, and on the other side by α1, α2 and α7, which showed the same structure as BAXs in mammals. The highest expression level of SpBAX was detected in hepatopancreas tissue. The expression level of SpBAX was up-regulated in 12 h in hepatopancreas and in 24 h in haemocytes after air exposure. Meanwhile, a flow cytometry assay revealed that the proportion of apoptotic haemocytes exceeded 65% after air exposure for 36 h, showing a significant difference with the control group. These results indicated that the cloned SpBAX might be involved in the response to air exposure stress by causing cell apoptosis. This study may thus be helpful to clarify the mechanisms of the air-exposure stress response in the mud crab.

The miRNAs profiling revealed by high-throughput sequencing upon WSSV infection in mud crab Scylla paramamosain

2020 ◽  
Vol 100 ◽  
pp. 427-435 ◽  
Author(s):  
Xin Ren ◽  
Shanmeng Lin ◽  
Tongtong Kong ◽  
Yi Gong ◽  
Hongyu Ma ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Scylla Paramamosain ◽  
Mud Crab

B-type allatostatin regulates immune response of hemocytes in mud crab Scylla paramamosain

2021 ◽  
Vol 120 ◽  
pp. 104050
Author(s):  
Zhanning Xu ◽  
Yujie Wei ◽  
Guizhong Wang ◽  
Haihui Ye
Keyword(s):  
Immune Response ◽  
Scylla Paramamosain ◽  
Mud Crab

Mud crab Scylla paramamosain glutamate dehydrogenase: molecular cloning, tissue expression and response to hyposmotic stress

2014 ◽  
Vol 81 (1) ◽  
pp. 175-186 ◽  
Author(s):  
Jing-Ying Lu ◽  
Miao-An Shu ◽  
Bing-Peng Xu ◽  
Guang-Xu Liu ◽  
You-Zhi Ma ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Glutamate Dehydrogenase ◽  
Tissue Expression ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Hyposmotic Stress

Effect of methyl farnesoate and farnesoic acid during 5th zoea and megalopa metamorphosis in the mud crab Scylla paramamosain Estampador, 1950 (Decapoda, Brachyura, Portunidae)

Crustaceana ◽  
2021 ◽  
Vol 94 (7) ◽  
pp. 855-863
Author(s):  
Ming Zhao ◽  
Fengying Zhang ◽  
Wei Wang ◽  
Zhiqiang Liu ◽  
Lingbo Ma
Keyword(s):  
Larval Development ◽  
Morphological Changes ◽  
Scylla Paramamosain ◽  
Dependent Manner ◽  
Mud Crab ◽  
Methyl Farnesoate ◽  
Concentration Dependent Manner ◽  
Low Levels ◽  
Juvenile Crab ◽  
Farnesoic Acid

Abstract The mud crab Scylla paramamosain is one of the economically important aquaculture species in China. The larval development of the mud crab is characterized by two significant morphological changes, from the 5th zoea (Z5) to the megalopa (M) stage and from the M to the first juvenile crab (C1) stage. In this study, we found that methyl farnesoate (MF) could prohibit the Z5 to M metamorphosis in a concentration-dependent manner, and that a concentration of 10 μM MF could completely prohibit the Z5 metamorphosis. Farnesoic acid (FA) could also prohibit the Z5 metamorphosis, but its effects seemed to be concentration-independent. In addition, MF could delay rather than prohibit the M to C1 metamorphosis, while FA had no effect on the M to C1 metamorphosis at all. To summarize, it is hypothesized that either absence of MF and FA, or at least very low levels of these substances, might be necessary for a successful Z5 to M metamorphosis.

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