The Brassica Napus Fatty Acid Exporter FAX1-1 Contributes to Biological Yield, Seed Oil Production, and Oil Quality

Abstract Background: In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil production, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not been explored. Results: Our genome-wide association study (GWAS) of 520 B. napus accessions identified the chloroplast membrane protein-localized FATTY ACID EXPORTER 1-1 (FAX1-1) as a candidate associated with biological yield. Seed transcript levels of BnaFAX1-1 were higher in a cultivar with high seed oil content relative to a low-oil cultivar. BnaFAX1-1 localized to the plastid envelope. When expressed in Arabidopsis thaliana, BnaFAX1-1 enhanced biological yield (total plant dry matter), seed yield and seed oil content per plant. Likewise, in the ﬁeld, B. napus BnaFAX1-1 overexpression lines (BnaFAX1-1-OE) displayed significantly enhanced biological yield, seed yield, and seed oil content compared with the wild type. BnaFAX1-1 overexpression also up-regulated gibberellic acid 4 (GA4) biosynthesis, which may contribute to biological yield improvement. Furthermore, oleic acid (C18:1) significantly increased in BnaFAX1-1 overexpression seeds. Conclusion: Our results indicated that the putative fatty acid exporter BnaFAX1-1 simultaneously improved seed oil production, oil quality and biological yield in B. napus, providing new approaches for future molecular breeding.

Download Full-text

The Brassica napus fatty acid exporter FAX1-1 contributes to biological yield, seed oil content, and oil quality

Biotechnology for Biofuels ◽

10.1186/s13068-021-02035-4 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Zhongchun Xiao ◽

Fang Tang ◽

Liyuan Zhang ◽

Shengting Li ◽

Shufeng Wang ◽

...

Keyword(s):

Fatty Acid ◽

Brassica Napus ◽

Seed Yield ◽

Oil Content ◽

Seed Oil ◽

Genome Wide Association Study ◽

Oil Quality ◽

Oilseed Crop ◽

Seed Oil Content ◽

Biological Yield

Abstract Background In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil content, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not been explored. Results Our genome-wide association study (GWAS) of 520 B. napus accessions identified the chloroplast membrane protein-localized FATTY ACID EXPORTER 1-1 (FAX1-1) as a candidate associated with biological yield. Seed transcript levels of BnaFAX1-1 were higher in a cultivar with high seed oil content relative to a low-oil cultivar. BnaFAX1-1 was localized to the plastid envelope. When expressed in Arabidopsis thaliana, BnaFAX1-1 enhanced biological yield (total plant dry matter), seed yield and seed oil content per plant. Likewise, in the field, B. napus BnaFAX1-1 overexpression lines (BnaFAX1-1-OE) displayed significantly enhanced biological yield, seed yield, and seed oil content compared with the wild type. BnaFAX1-1 overexpression also up-regulated gibberellic acid 4 (GA4) biosynthesis, which may contribute to biological yield improvement. Furthermore, oleic acid (C18:1) significantly increased in BnaFAX1-1 overexpression seeds. Conclusion Our results indicated that the putative fatty acid exporter BnaFAX1-1 may simultaneously improve seed oil content, oil quality and biological yield in B. napus, providing new approaches for future molecular breeding.

Download Full-text

Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus

The Plant Journal ◽

10.1111/tpj.15003 ◽

2020 ◽

Vol 104 (5) ◽

pp. 1410-1422

Author(s):

Shan Tang ◽

Dong‐Xu Liu ◽

Shaoping Lu ◽

Liangqian Yu ◽

Yuqing Li ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Brassica Napus ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content

Download Full-text

QTL analysis of an intervarietal set of substitution lines in Brassica napus: (i) Seed oil content and fatty acid composition

Heredity ◽

10.1038/sj.hdy.6800176 ◽

2003 ◽

Vol 90 (1) ◽

pp. 39-48 ◽

Cited By ~ 102

Author(s):

M J Burns ◽

S R Barnes ◽

J G Bowman ◽

M H E Clarke ◽

C P Werner ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Brassica Napus ◽

Acid Composition ◽

Qtl Analysis ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content ◽

Substitution Lines

Download Full-text

Identification of candidate genes controlling oil content by combination of genome-wide association and transcriptome analysis in the oilseed crop Brassica napus

Biotechnology for Biofuels ◽

10.1186/s13068-019-1557-x ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 6

Author(s):

Zhongchun Xiao ◽

Chao Zhang ◽

Fang Tang ◽

Bo Yang ◽

Liyuan Zhang ◽

...

Keyword(s):

Brassica Napus ◽

Candidate Genes ◽

Oil Content ◽

Seed Oil ◽

Significant Snps ◽

Genome Wide Association ◽

Oilseed Crop ◽

Seed Oil Content ◽

Genome Wide ◽

A Genome

Abstract Background Increasing seed oil content is one of the most important targets for rapeseed (Brassica napus) breeding. However, genetic mechanisms of mature seed oil content in Brassica napus (B. napus) remain little known. To identify oil content-related genes, a genome-wide association study (GWAS) was performed using 588 accessions. Results High-throughput genome resequencing resulted in 385,692 high-quality single nucleotide polymorphism (SNPs) with a minor allele frequency (MAF) > 0.05. We identified 17 loci that were significantly associated with seed oil content, among which 12 SNPs were distributed on the A3 (11 loci) and A1 (one loci) chromosomes, and five novel significant SNPs on the C5 (one loci) and C7 (four loci) chromosomes, respectively. Subsequently, we characterized differentially expressed genes (DEGs) between the seeds and silique pericarps on main florescences and primary branches of extremely high- and low-oil content accessions (HO and LO). A total of 64 lipid metabolism-related DEGs were identified, 14 of which are involved in triacylglycerols (TAGs) biosynthesis and assembly. Additionally, we analyzed differences in transcription levels of key genes involved in de novo fatty acid biosynthesis in the plastid, TAGs assembly and lipid droplet packaging in the endoplasmic reticulum (ER) between high- and low-oil content B. napus accessions. Conclusions The combination of GWAS and transcriptome analyses revealed seven candidate genes located within the confidence intervals of significant SNPs. Current findings provide valuable information for facilitating marker-based breeding for higher seed oil content in B. napus.

Download Full-text

Increasing seed oil content and altering oil quality of Brassica napus L. by over-expression of diacylglycerol acyltransferase 1 (SsDGAT1) from Sapium sebiferum (L.) Roxb.

Molecular Breeding ◽

10.1007/s11032-016-0543-2 ◽

2016 ◽

Vol 36 (10) ◽

Cited By ~ 9

Author(s):

Dan Peng ◽

Lin Zhang ◽

Xiaofeng Tan ◽

Deyi Yuan ◽

Xuanming Liu ◽

...

Keyword(s):

Brassica Napus ◽

Oil Content ◽

Seed Oil ◽

Oil Quality ◽

Diacylglycerol Acyltransferase ◽

Sapium Sebiferum ◽

Seed Oil Content ◽

Brassica Napus L ◽

Over Expression

Download Full-text

Identification of QTL influencing seed oil content, fatty acid profile and days to flowering in Brassica napus L.

Euphytica ◽

10.1007/s10681-015-1565-2 ◽

2015 ◽

Vol 207 (1) ◽

pp. 191-211 ◽

Cited By ~ 19

Author(s):

Nasir Javed ◽

Jianfeng Geng ◽

Muhammad Tahir ◽

P. B. E. McVetty ◽

Genyi Li ◽

...

Keyword(s):

Fatty Acid ◽

Brassica Napus ◽

Fatty Acid Profile ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content ◽

Brassica Napus L ◽

Days To Flowering

Download Full-text

Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in Brassica napus L.

International Journal of Molecular Sciences ◽

10.3390/ijms22031033 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1033

Author(s):

Abirami Rajavel ◽

Selina Klees ◽

Johanna-Sophie Schlüter ◽

Hendrik Bertram ◽

Kun Lu ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factors ◽

Brassica Napus ◽

Oil Content ◽

Seed Oil ◽

Seed Oil Content ◽

Complex Interplay ◽

Brassica Napus L ◽

Data Set ◽

Developmental Switches

Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.

Download Full-text

Cold-pressed cactus pear seed oil: Quality and stability

Grasas y Aceites ◽

10.3989/gya.0329201 ◽

2021 ◽

Vol 72 (3) ◽

pp. e415 ◽

Cited By ~ 3

Author(s):

M. De Wit ◽

V.K. Motsamai ◽

A. Hugo

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Physicochemical Properties ◽

Acid Composition ◽

Oil Content ◽

Seed Oil ◽

Oil Quality ◽

Cactus Pear ◽

Cold Pressed

Cold-pressed seed oil from twelve commercially produced cactus pear cultivars was assessed for oil yield, fatty acid composition, physicochemical properties, quality and stability. Large differences in oil content, fatty acid composition and physicochemical properties (IV, PV, RI, tocopherols, ORAC, % FFA, OSI and induction time) were observed. Oil content ranged between 2.51% and 5.96% (Meyers and American Giant). The important fatty acids detected were C16:0, C18:0, C18:1c9 and C18:2c9,12, with C18:2c9,12, the dominating fatty acid, ranging from 58.56-65.73%, followed by C18:1c9, ranging between 13.18-16.07%, C16:0, which ranged between 10.97 - 15.07% and C18:0, which ranged between 2.62-3.18%. Other fatty acids such as C14:0, C16:1c9, C17:0, C17:1c10, C20:0, C18:3c9,12,15 and C20:3c8,11,14 were detected in small amounts. The quality parameters of the oils were strongly influenced by oil content, fatty acid composition and physicochemical properties. Oil content, PV, % FFA, RI, IV, tocopherols, ORAC and ρ-anisidine value were negatively correlated with OSI. C18:0; C18:1c9; C18:2c9,12; MUFA; PUFA; n-6 and PUFA/SFA were also negatively correlated with OSI. Among all the cultivars, American Giant was identified as the paramount cultivar with good quality traits (oil content and oxidative stability).

Download Full-text