Organic-Inorganic Fertilization Built Higher Stability of Soil And Root Microbial Networks Than Exclusive Mineral Or Organic Fertilization
Abstract BackgroundRoot microbiome is critical for plant health and performance. Many studies have assessed the impact of agricultural management on soil microbiome. But a comprehensive understanding of how root microbiota is affected by soil types and fertilization is still lacking. It is clear yet whether the stability of root microbiome is affected by fertilization regimes, and whether in the same patterns as soil microbiome. MethodsWe conducted a long-term experiment and investigated the impact of soil type, plant type and fertilization regimes on soil and root bacterial communities using high-throughput sequencing and network analysis.ResultsOur results indicated that microbial network under combined organic-inorganic fertilization had higher stability than exclusive inorganic or organic fertilizer. In addition, fertilization exhibited stronger effects on root microbiome than on soil microbiome. While total nitrogen mainly contributes to the variance of root microbiome, pH and soil organic matter were responsible for the differences of soil microbiome. Bacteroidetes and Firmicutes appeared as important drivers in soil and root microbiome amended with organic fertilizer, whereas Actinobacteria was enriched in the soil microbiome under inorganic fertilizer. ConclusionsOur results clearly indicated the responsive shifts of soil and root microbiome to different fertilization regimes, and gave hints for developing better fertilization practices and establishing healthy root associated microbiota.