scholarly journals Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity

2020 ◽  
Author(s):  
Mariya Nikolaeva ◽  
Andrey Pavlovich Momot ◽  
Marina Sabirovna Zainulina ◽  
Natalia Nikolaevna Yasafova ◽  
Irina Alekseevna Taranenko
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Main Group ◽  
Trophoblast Invasion ◽  
Control Group ◽  
G20210a Mutation ◽  
Factor Ii ◽  
Invasion Waves ◽  
In Blood Plasma

Abstract Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

scholarly journals Pregnancy complications in G20210A mutation carriers associated with high prothrombin activity

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
M. G. Nikolaeva ◽  
A. P. Momot ◽  
M. S. Zainulina ◽  
N. N. Yasafova ◽  
I. A. Taranenko
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Main Group ◽  
Trophoblast Invasion ◽  
Control Group ◽  
G20210a Mutation ◽  
Factor Ii ◽  
Invasion Waves ◽  
In Blood Plasma

Abstract Objective To study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes. Material and methods A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7 ± 4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out. Results In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130–150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149 to 181% [95% CI 142–195], p < 0.0001. With Factor II activity ranging from 148.5 to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR). Conclusion The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor’s level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p < 0.0001) and within 7–8 weeks of gestation it was 181.3% (AUC – 0.84; p < 0.0001).

scholarly journals Pregnancy Complications In G20210A Mutation Carriers Associated With High Prothrombin Activity

2020 ◽  
Author(s):  
Mariya Nikolaeva ◽  
Andrey Pavlovich Momot ◽  
Marina Sabirovna Zainulina ◽  
Natalia Nikolaevna Yasafova ◽  
Irina Alekseevna Taranenko
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Main Group ◽  
Trophoblast Invasion ◽  
Control Group ◽  
G20210a Mutation ◽  
Factor Ii ◽  
Invasion Waves ◽  
In Blood Plasma

Abstract Objective to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes. Material and methods A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out. Results In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR). Conclusion The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

scholarly journals Gestational complications of the prothrombin G20210A mutation related to prothrombin activity

2020 ◽  
Vol 14 (2) ◽  
pp. 192-202
Author(s):  
M. G. Nikolaeva ◽  
N. N. Yasafova ◽  
A. P. Momot ◽  
M. S. Zainulina ◽  
K. A. Momot ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Prothrombin G20210a ◽  
Control Group ◽  
Study Group ◽  
Fetal Growth Retardation ◽  
Prothrombin Activity ◽  
G20210a Mutation ◽  
Factor Ii

Aim: to study the association between prothrombin activity in the blood plasma and gestational complications in women with the prothrombin G20210A mutation.Materials and methods. A prospective clinical cohort study including 290 pregnant women aged 18 to 45 years was conducted from 2012 to 2018. Two cohorts were formed: a study group of 140 patients with the GA genotype and a control group of 150 women with the GG genotype. In the groups, the activity of prothrombin (Factor II) in the venous blood plasma was evaluated during pregnancy. The stages of cytotrophoblast invasion were taken into account when relating the prothrombin activity to gestational complications.Results. The median prothrombin activity in the control group ranged from 108 % during the preconception period to 144 % during pregnancy (95 % CI = 130–150). In the study group with the GA genotype, the activity was significantly higher at the same periods: from 149 to 181 % (95 % CI = 142–195; p < 0.0001). With the prothrombin activity from 148.5 to 180.6 %, the pregnancy in the study group progressed normally. Higher levels of prothrombin activity were associated with early and/or severe preeclampsia (PE), and fetal growth retardation (FGR).Conclusion. The obtained data on prothrombin activity in the blood plasma during pregnancy complications suggest that the manifestation of the GA genotype in the form of early and/or severe PE and FGR is associated with the level of plasma prothrombin activity. The threshold value of Factor II activity was calculated for patients with the G20210A mutation; based on this value it becomes possible to predict PE at the preconception stage (171.0 %; AUC – 0.86; p < 0.0001) and at a gestational age of 7–8 weeks (181.3 %; AUC – 0.84; p < 0.0001).

scholarly journals Application of sorbitol-based infusion preparation for protein-protecting effect in surgery patients with middle breast cancer

2020 ◽  
pp. 62-62
Author(s):  
S.V. Prymak ◽  
B.R. Dzis ◽  
R.P. Dzis ◽  
V.L. Novak ◽  
M.P. Dzisiv ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Main Group ◽  
The Body ◽  
Control Group ◽  
Thoracic Esophagus ◽  
Energy Effect ◽  
Plasma Urea ◽  
Patients With Cancer ◽  
Middle Thoracic Esophagus ◽  
In Blood Plasma

Objective. To study the energy effect of infusions of the drug Sorbilact in the body of operated patients with cancer of the middle thoracic esophagus. Materials and methods. Clinical use of the drug Sorbilact was performed in 30 operated patients with cancer of the middle thoracic esophagus (the main group). Sorbilact was administered intravenously immediately after surgery, drip at a rate of 30 drops per minute for 5 days at a dose of 800 ml per day. Plasma urea concentration was determined before infusions on the 1st day after surgery, and on days 2, 3, 5 after Sorbilact infusions. The control group – 25 operated patients – was administered 5 % glucose solution at a dose of 800.0 ml. Results and discussion. In the first days after surgery in patients with cancer of the middle thoracic esophagus an increase in the concentration of urea in blood plasma was revealed. In the main group of operated patients after resection of the middle thoracic esophagus in repeated studies on the 2nd, 3rd, 5th day after infusions a significant decrease in the concentration of urea in blood plasma was showed. It indicates that less endogenous protein is consumed for energy purposes, and the caloric needs of the operated body are covered by intensive absorption of Sorbilact, which reduces the catabolism of internal proteins, resulting in reduced nitrogen production, which is manifested in a decrease in the concentration of urea in blood plasma and its difference. In the control group of operated patients in repeated studies on the 2nd, 3rd, 5th day after infusions an increase in plasma urea was revealed, which indicates insufficient caloric energy expenditure of the operated organism. Conclusions. Intravenous infusions of the drug Sorbilact according to the indicator of increment of urea in blood plasma in operated patients after resection of the middle thoracic esophagus in the early postoperative period show a pronounced energy effect.

Markers of endothelium dysfunction in pregnant women with varicose veins of the lower extremities

2017 ◽  
pp. 75-77
Author(s):  
V.O. Benyuk ◽  
◽  
S.D. Koval ◽  
V.V. Kurochka ◽  
T.V. Kovalyuk ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Pregnant Women ◽  
Comparison Group ◽  
Varicose Veins ◽  
Lower Extremities ◽  
Main Group ◽  
Control Group ◽  
Endothelium Dysfunction ◽  
Complex Therapy ◽  
In Blood Plasma

The objective: evaluation of the impact of the complex therapy on indexes of dysfunction of endothelium homocysteine and fibronectin in pregnant women with varicose veins of lower extremities. Patients and methods. 80 pregnant women with varicose veins of the lower extremities before and after treatment underwent the determination of the indexes of homocysteine and fibronectin. The main group – 42 pregnant women with varicose veins of the lower extremities received complex therapy within 30-36 weeks (medication with angioprotective effect, direct anticoagulant; graduated elastic compression; flavonoid with immunotropic effect); comparison group – 38 women with varicose veins of the lower extremities, received local heparin-contenting medication. The control group included 50 pregnant women without VVLE. Results. In analysis of the concentration of the homocysteine in blood plasma in pregnant women with varicose veins of the lower extremities the increase of the level was detected in 38% and 28.9% of the patients of the main group and comparison group accordingly; in control group – only in 2%. In main group the content of fibronectin was elevated in 69%; in comparison group in 71% of the patients, in control group – only in 6%. After treatment, the assessment of the markers of the endothelium dysfunction in examined groups was performed. The content of homocysteine decreased in 1.7 times in main group and only in 0.8% times in comparison group. The average level of fibronectin in main group decreased on 31.2% in comparison with benchmark; in comparison group its level decreased only on 12.2%. Conclusions. Thereby, we detected in third trimester in pregnant women with varicose veins of the lower extremities statistically credibly high indexes of homocysteine and fibronectin compared to the control group. Decrease of the level of homocysteine in 1.7 times and content of fibronectin in blood plasma in 1.5 times can be a sign of positive stabilizing effect on endothelium of blood vessels in suggested therapy. Key words: varicose veins of lower extremities, homocysteine, fibronectin, complex therapy.

scholarly journals Endothelial Cell Markers in Patients with Pseudoexfoliation Syndrome

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Joanna Stafiej ◽  
Grażyna Malukiewicz ◽  
Hanna Lesiewska-Junk ◽  
Danuta Rość ◽  
Karolina Kaźmierczak
Keyword(s):  
Endothelial Cell ◽  
Blood Plasma ◽  
Venous Blood ◽  
Pseudoexfoliation Syndrome ◽  
Antihypertensive Drug Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Ophthalmological Examination ◽  
Cell Markers ◽  
In Blood Plasma

The aim of the study was the assessment of the von Willebrand antigen (vWF Ag), E-selectin, and P-selectin concentration in blood plasma of patients with pseudoexfoliation syndrome (PEX). The group studied comprised 30 patients with PEX, aged from 50 to 86 years (mean 73, SD 8 years). Patients with cardiovascular and cerebrovascular diseases, diabetes mellitus, infectious disease, cancer, renal or liver insufficiency, connective tissue disease, current smoking, and hormone, antiplatelet, hypolipidemic, antioxidant, or antihypertensive drug therapy were excluded from the study. Each subject underwent a complete ophthalmological examination. Venous blood samples from the cubital vein were taken into sodium citrate solution. VWF Ag, sP-selectin, and sE-selectin concentration were determined by a commercially available enzyme-linked immunosorbent assay (MedSystems, Diagnostica Stago/Roche, R&D). Concentrations of vWF Ag, soluble E-selectin, and soluble P-selectin in blood plasma in the study group were compared with the levels in blood plasma in the control group. No significant differences were found between the groups. Our results indicate that there might be no correlation between PEX and such endothelial cell markers as vWF Ag, sP-selectin, and sE-selectin concentrations. Since the study size is limited, further investigations to confirm that there is no association between endothelial dysfunction in PEX and risk of future cardiovascular disease are necessary.

"ULtra-clean" isotope diultion/mass spectrometic analyses for lead in human blood plasma indicated that most reported values are artificially high.

1980 ◽  
Vol 26 (11) ◽  
pp. 1603-1607 ◽  
Author(s):  
J Everson ◽  
C C Patterson
Keyword(s):  
Blood Plasma ◽  
Preliminary Data ◽  
Venous Blood ◽  
Human Blood Plasma ◽  
High Exposure ◽  
Large Factor ◽  
Lead Intake ◽  
Mass Spectrometric Techniques ◽  
Clean Laboratory ◽  
In Blood Plasma

Abstract We measured lead concentrations in venous blood plasma from two subjects, one having a typical exposure and the other a high exposure to lead. Our preliminary data, obtained by isotope dilution/mass spectrometric techniques in an ultra-clean laboratory, show lead concentrations of 0.02 mug/L and 2 mug/L, respectively, in their blood plasma, and 110 mug/L and 800 mug/L, respectively, in samples of whole blood. These results indicate that plasma lead concentrations previously reported have been overestimated by a large factor, and that further improvements in analytical procedures are needed in most laboratories before data on lead concentratios in blood plasma can be properly interpreted. Our preliminary data indicate a positive correlation between lead intake and lead concentrations in blood plasma.

Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

2016 ◽  
Vol 69 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Milan Dastych Jr. ◽  
Michal Šenkyřík ◽  
Milan Dastych ◽  
František Novák ◽  
Petr Wohl ◽  
...  
Keyword(s):  
Trace Elements ◽  
Parenteral Nutrition ◽  
Blood Plasma ◽  
Whole Blood ◽  
Statistical Significance ◽  
Home Parenteral Nutrition ◽  
Control Group ◽  
The Status ◽  
In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

scholarly journals Etiopathogenesis of Sheehan’s Syndrome: Roles of Coagulation Factors and TNF-Alpha

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Halit Diri ◽  
Elif Funda Sener ◽  
Fahri Bayram ◽  
Nazife Tascioglu ◽  
Yasin Simsek ◽  
...  
Keyword(s):  
Gene Polymorphisms ◽  
Venous Blood ◽  
Hormone Deficiency ◽  
Control Group ◽  
Pituitary Hormone ◽  
Factor V ◽  
Sheehan’S Syndrome ◽  
Factor Ii ◽  
Sheehan's Syndrome ◽  
Pai 1

Sheehan’s Syndrome (SS) is defined as pituitary hormone deficiency due to ischemic infarction of the pituitary gland as a result of massive postpartum uterine hemorrhage. Herein, we aimed to investigate the roles ofFactor II(G20210A),Factor V(G1691A),MTHFR(C677T and A1298C),PAI-14G/5G, andTNF-α(-308  G>A) gene polymorphisms in the etiopathogenesis of SS. Venous blood samples were obtained from 53 cases with SS and 43 healthy women. Standard methods were used to extract the genomic DNAs.Factor II(G20210A),Factor V(G1691A), andMTHFR(C677T and A1298C) polymorphisms were identified by real-time PCR.PAI-14G/5G andTNF-α(-308  G>A) gene polymorphisms were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. According to statistical analysis, none of the polymorphisms were found to be significantly higher in the SS group compared to the control group. Hence, we suggest that genetic factors other thanFactor II, Factor V, MTHFR, PAI-1, andTNF-αgene polymorphisms should be researched in the etiopathogenesis of SS.

scholarly journals PENGARUH PEMBERIAN TIMBAL (Pb) DOSIS KRONIS SECARA ORAL TERHADAP PENINGKATAN PENANDA KERUSAKAN ORGAN PADA MENCIT

el–Hayah ◽  
2001 ◽  
Vol 3 (1) ◽  
Author(s):  
Abdul Malik Setiawan
Keyword(s):  
Heavy Metals ◽  
Blood Plasma ◽  
Toxic Substance ◽  
Lead Toxicity ◽  
Control Group ◽  
High Dose ◽  
Lead In Blood ◽  
Research Experiment ◽  
Medium Dose ◽  
In Blood Plasma

<p>Several kind of heavy metals that present in the environment are considered as toxic substance to human and animal. Lead (Pb) is one of  heavy metals that increase in use for the last decade. Lead toxicity to human had wide influence in medical aspect, from nerve problem, bone metabolics disturbance until liver and renal failure. This experiment tried to found out the effect of cronic oral lead consupmtion to lead plasma rate in mice. Design of this experiment is true research experiment to test wheater there is an effect of oral lead consumption to acumulation of   lead in blood plasma of mice. The dose devided to two kinds, medium dose (50 ppm) and high dose (100 ppm). The results show increase of lead accumulation in blood plasma of the mice compared to control group.</p> <br />

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