Application of sorbitol-based infusion preparation for protein-protecting effect in surgery patients with middle breast cancer

Objective. To study the energy effect of infusions of the drug Sorbilact in the body of operated patients with cancer of the middle thoracic esophagus. Materials and methods. Clinical use of the drug Sorbilact was performed in 30 operated patients with cancer of the middle thoracic esophagus (the main group). Sorbilact was administered intravenously immediately after surgery, drip at a rate of 30 drops per minute for 5 days at a dose of 800 ml per day. Plasma urea concentration was determined before infusions on the 1st day after surgery, and on days 2, 3, 5 after Sorbilact infusions. The control group – 25 operated patients – was administered 5 % glucose solution at a dose of 800.0 ml. Results and discussion. In the first days after surgery in patients with cancer of the middle thoracic esophagus an increase in the concentration of urea in blood plasma was revealed. In the main group of operated patients after resection of the middle thoracic esophagus in repeated studies on the 2nd, 3rd, 5th day after infusions a significant decrease in the concentration of urea in blood plasma was showed. It indicates that less endogenous protein is consumed for energy purposes, and the caloric needs of the operated body are covered by intensive absorption of Sorbilact, which reduces the catabolism of internal proteins, resulting in reduced nitrogen production, which is manifested in a decrease in the concentration of urea in blood plasma and its difference. In the control group of operated patients in repeated studies on the 2nd, 3rd, 5th day after infusions an increase in plasma urea was revealed, which indicates insufficient caloric energy expenditure of the operated organism. Conclusions. Intravenous infusions of the drug Sorbilact according to the indicator of increment of urea in blood plasma in operated patients after resection of the middle thoracic esophagus in the early postoperative period show a pronounced energy effect.

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Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity

10.21203/rs.3.rs-67776/v1 ◽

2020 ◽

Author(s):

Mariya Nikolaeva ◽

Andrey Pavlovich Momot ◽

Marina Sabirovna Zainulina ◽

Natalia Nikolaevna Yasafova ◽

Irina Alekseevna Taranenko

Keyword(s):

Blood Plasma ◽

Venous Blood ◽

Threshold Value ◽

Main Group ◽

Trophoblast Invasion ◽

Control Group ◽

G20210a Mutation ◽

Factor Ii ◽

Invasion Waves ◽

In Blood Plasma

Abstract Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

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Pregnancy complications in G20210A mutation carriers associated with high prothrombin activity

Thrombosis Journal ◽

10.1186/s12959-021-00289-4 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

M. G. Nikolaeva ◽

A. P. Momot ◽

M. S. Zainulina ◽

N. N. Yasafova ◽

I. A. Taranenko

Keyword(s):

Blood Plasma ◽

Venous Blood ◽

Threshold Value ◽

Main Group ◽

Trophoblast Invasion ◽

Control Group ◽

G20210a Mutation ◽

Factor Ii ◽

Invasion Waves ◽

In Blood Plasma

Abstract Objective To study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes. Material and methods A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7 ± 4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out. Results In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130–150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149 to 181% [95% CI 142–195], p < 0.0001. With Factor II activity ranging from 148.5 to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR). Conclusion The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor’s level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p < 0.0001) and within 7–8 weeks of gestation it was 181.3% (AUC – 0.84; p < 0.0001).

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Pregnancy Complications In G20210A Mutation Carriers Associated With High Prothrombin Activity

10.21203/rs.3.rs-67776/v2 ◽

2020 ◽

Author(s):

Mariya Nikolaeva ◽

Andrey Pavlovich Momot ◽

Marina Sabirovna Zainulina ◽

Natalia Nikolaevna Yasafova ◽

Irina Alekseevna Taranenko

Keyword(s):

Blood Plasma ◽

Venous Blood ◽

Threshold Value ◽

Main Group ◽

Trophoblast Invasion ◽

Control Group ◽

G20210a Mutation ◽

Factor Ii ◽

Invasion Waves ◽

In Blood Plasma

Abstract Objective to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes. Material and methods A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out. Results In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR). Conclusion The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

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The effect of silicon compounds on hematological indicators and content of lipids in blood of rabbits

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9240 ◽

2018 ◽

Vol 20 (92) ◽

pp. 190-196 ◽

Cited By ~ 1

Author(s):

A. I. Ivanitskaya ◽

Ya. V. Lesyk

Keyword(s):

Body Weight ◽

Blood Plasma ◽

Fractional Composition ◽

The Body ◽

Control Group ◽

Free Access ◽

Animal Blood ◽

Preparatory Period ◽

Experimental Group ◽

In Blood Plasma

The aim of the study was to find out the effect of casting of citrate nanosіlicon absorption and metasilicate sodium on the hematological parameters and lipid content and their fractional composition in blood plasma preparatory period 10 days prior to insemination and 20 days of lactation. The research was carried out on 60 rabbits of the Hyla hybrid, divided into three groups (control and two experimental), with 20 animals in each. The control animals were fed without limitation full-grain granulated feed with free access to water. Animals of the first experimental group (E-I) fed the diet of the control group and during the course of the day poured out the silicon citrate obtained using the nanotechnology method, 50 mkg Si/kg body weight with water. Samples of the second experimental group (E-II) feed the diet of the control group and set the sodium metasilicate (Na2SiO3H2O) 2.5 mg Si/kg body weight with water. The trial lasted 95 days, including the preparatory period of 10 days, the trial – 85 days. In the preparatory period of 10 days from the beginning of the study and in a trial of 20 days of lactation (65 days of supplements) in the rabbits, samples of blood from the marginal ear vein were taken. Hematologic studies were performed using the automatic hematologic analyzer “Orphee Mythic 18”, in the blood plasma, the content of total lipids and their fractional composition were determined. It was established that the number of erythrocytes and hemoglobin concentration in the blood of the rabbits of experimental group, which were given silicon citrate, were respectively higher by 15.2% (P < 0.05) and 12.0% (P < 0.01) by 20 days lactation versus control. This may indicate a more pronounced effect of the organic compound of silicon on the hematopoietic function of the body of the rabbits. According to white blood, the differences from control were found during the presentation of supplements with higher (P < 0.05) changes in 20 days of lactation in animal blood and experimental group. It was noted that the content of triacylglycerols in plasma of blood of groups I and II was lower by 31.2 and 32.8%, respectively (P < 0.05) compared to control. In blood plasma of animals in experimental groups an increase in the fractions of phosphatidylcholine, sphingomyelin and lysolecetin was observed compared to control. Such a redistribution was due to the reduction of the fractions of phosphatidic acid, phosphatidyl ethanolamine and phosphatidylinositol, and apparently, is associated with changes in the activity of the enzymes concerned. Hematological studies and determination of the fractional composition of lipids and phospholipids of blood plasma of the rabbits show positive changes that contribute to the metabolic accumulation of energy and plastic components of their organism.

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Markers of endothelium dysfunction in pregnant women with varicose veins of the lower extremities

HEALTH OF WOMAN ◽

10.15574/hw.2017.120.75 ◽

2017 ◽

pp. 75-77

Author(s):

V.O. Benyuk ◽

◽

S.D. Koval ◽

V.V. Kurochka ◽

T.V. Kovalyuk ◽

...

Keyword(s):

Blood Plasma ◽

Pregnant Women ◽

Comparison Group ◽

Varicose Veins ◽

Lower Extremities ◽

Main Group ◽

Control Group ◽

Endothelium Dysfunction ◽

Complex Therapy ◽

In Blood Plasma

The objective: evaluation of the impact of the complex therapy on indexes of dysfunction of endothelium homocysteine and fibronectin in pregnant women with varicose veins of lower extremities. Patients and methods. 80 pregnant women with varicose veins of the lower extremities before and after treatment underwent the determination of the indexes of homocysteine and fibronectin. The main group – 42 pregnant women with varicose veins of the lower extremities received complex therapy within 30-36 weeks (medication with angioprotective effect, direct anticoagulant; graduated elastic compression; flavonoid with immunotropic effect); comparison group – 38 women with varicose veins of the lower extremities, received local heparin-contenting medication. The control group included 50 pregnant women without VVLE. Results. In analysis of the concentration of the homocysteine in blood plasma in pregnant women with varicose veins of the lower extremities the increase of the level was detected in 38% and 28.9% of the patients of the main group and comparison group accordingly; in control group – only in 2%. In main group the content of fibronectin was elevated in 69%; in comparison group in 71% of the patients, in control group – only in 6%. After treatment, the assessment of the markers of the endothelium dysfunction in examined groups was performed. The content of homocysteine decreased in 1.7 times in main group and only in 0.8% times in comparison group. The average level of fibronectin in main group decreased on 31.2% in comparison with benchmark; in comparison group its level decreased only on 12.2%. Conclusions. Thereby, we detected in third trimester in pregnant women with varicose veins of the lower extremities statistically credibly high indexes of homocysteine and fibronectin compared to the control group. Decrease of the level of homocysteine in 1.7 times and content of fibronectin in blood plasma in 1.5 times can be a sign of positive stabilizing effect on endothelium of blood vessels in suggested therapy. Key words: varicose veins of lower extremities, homocysteine, fibronectin, complex therapy.

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Plasma radio-metabolite analysis of PET tracers for dynamic PET imaging: TLC and autoradiography

EJNMMI Research ◽

10.1186/s13550-020-00705-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Fiona Li ◽

Justin W. Hicks ◽

Lihai Yu ◽

Lise Desjardin ◽

Laura Morrison ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Blood Plasma ◽

Kinetic Parameters ◽

High Performance ◽

The Body ◽

Accurate Estimation ◽

Dynamic Pet ◽

Arterial Concentration ◽

In Blood Plasma

Abstract Background In molecular imaging with dynamic PET, the binding and dissociation of a targeted tracer is characterized by kinetics modeling which requires the arterial concentration of the tracer to be measured accurately. Once in the body the radiolabeled parent tracer may be subjected to hydrolysis, demethylation/dealkylation and other biochemical processes, resulting in the production and accumulation of different metabolites in blood which can be labeled with the same PET radionuclide as the parent. Since these radio-metabolites cannot be distinguished by PET scanning from the parent tracer, their contribution to the arterial concentration curve has to be removed for the accurate estimation of kinetic parameters from kinetic analysis of dynamic PET. High-performance liquid chromatography has been used to separate and measure radio-metabolites in blood plasma; however, the method is labor intensive and remains a challenge to implement for each individual patient. The purpose of this study is to develop an alternate technique based on thin layer chromatography (TLC) and a sensitive commercial autoradiography system (Beaver, Ai4R, Nantes, France) to measure radio-metabolites in blood plasma of two targeted tracers—[18F]FAZA and [18F]FEPPA, for imaging hypoxia and inflammation, respectively. Results Radioactivity as low as 17 Bq in 2 µL of pig’s plasma can be detected on the TLC plate using autoradiography. Peaks corresponding to the parent tracer and radio-metabolites could be distinguished in the line profile through each sample (n = 8) in the autoradiographic image. Significant intersubject and intra-subject variability in radio-metabolites production could be observed with both tracers. For [18F]FEPPA, 50% of plasma activity was from radio-metabolites as early as 5-min post injection, while for [18F]FAZA, significant metabolites did not appear until 50-min post. Simulation study investigating the effect of radio-metabolite in the estimation of kinetic parameters indicated that 32–400% parameter error can result without radio-metabolites correction. Conclusion TLC coupled with autoradiography is a good alternative to high-performance liquid chromatography for radio-metabolite correction. The advantages of requiring only small blood samples (~ 100 μL) and of analyzing multiple samples simultaneously, make the method suitable for individual dynamic PET studies.

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MicroRNA-146a – the predictor of the development of metastases of choroidal melanoma

POINT OF VIEW EAST – WEST ◽

10.25276/2410-1257-2021-1-13-16 ◽

2021 ◽

pp. 13-16

Author(s):

A.F. Brovkina ◽

◽

N.D. Tsybikova ◽

◽

...

Keyword(s):

Blood Plasma ◽

Choroidal Melanoma ◽

Hematogenous Metastasis ◽

Control Group ◽

Regulate Gene Expression ◽

Patients With Cancer ◽

Cell Life ◽

Proliferation And Apoptosis ◽

Aggressive Tumor ◽

Cancer Of The Lung

MicroRNAs (miRNA, miR) - regulate gene expression, take part in the regulation of cell life, proliferation and apoptosis. There are not enough publications on the study of microRNA in the plasma of patients with choroidal melanoma (CM) and the presented data are contradictory. An increase in the level of microRNA-146a in blood plasma was noted in patients with cancer of the lung, esophagus and breast. In the present study, we used microRNA-146a in the blood plasma of patients with CM. Purpose. To determine the possibility and reliability of detecting microRNA-146a in the blood plasma of patients with CM, taking into account the localization of the tumor and its size. Material and methods. The blood plasma of 84 patients was studied mean age 63.4±1.2 years. A control group of 28 people who did not have tumor or chronic autoimmune diseases mean age 63.25±1.43 years. The expression level of miRNA-146a was determined by quantitative PCR. Results. An increase in the level of microRNA-146a was shown, which correlates with the localization and thickness of the tumor. The results were confirmed by statistical analysis. Conclusion. MicroRNA-146a, which increases in plasma levels in patients with CM as its size increases, can be used as a biomarker of aggressive tumor course in terms of hematogenous metastasis. Key words: choroidal melanoma, microRNA-146a, biomarkers.

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Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

Annals of Nutrition and Metabolism ◽

10.1159/000450763 ◽

2016 ◽

Vol 69 (2) ◽

pp. 120-124 ◽

Cited By ~ 2

Author(s):

Milan Dastych Jr. ◽

Michal Šenkyřík ◽

Milan Dastych ◽

František Novák ◽

Petr Wohl ◽

...

Keyword(s):

Trace Elements ◽

Parenteral Nutrition ◽

Blood Plasma ◽

Whole Blood ◽

Statistical Significance ◽

Home Parenteral Nutrition ◽

Control Group ◽

The Status ◽

In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

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PENGARUH PEMBERIAN TIMBAL (Pb) DOSIS KRONIS SECARA ORAL TERHADAP PENINGKATAN PENANDA KERUSAKAN ORGAN PADA MENCIT

el–Hayah ◽

10.18860/elha.v3i1.2215 ◽

2001 ◽

Vol 3 (1) ◽

Author(s):

Abdul Malik Setiawan

Keyword(s):

Heavy Metals ◽

Blood Plasma ◽

Toxic Substance ◽

Lead Toxicity ◽

Control Group ◽

High Dose ◽

Lead In Blood ◽

Research Experiment ◽

Medium Dose ◽

In Blood Plasma

<p>Several kind of heavy metals that present in the environment are considered as toxic substance to human and animal. Lead (Pb) is one of heavy metals that increase in use for the last decade. Lead toxicity to human had wide influence in medical aspect, from nerve problem, bone metabolics disturbance until liver and renal failure. This experiment tried to found out the effect of cronic oral lead consupmtion to lead plasma rate in mice. Design of this experiment is true research experiment to test wheater there is an effect of oral lead consumption to acumulation of lead in blood plasma of mice. The dose devided to two kinds, medium dose (50 ppm) and high dose (100 ppm). The results show increase of lead accumulation in blood plasma of the mice compared to control group.</p> <br />

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Surgical treatment of acetabular fractures

TRAUMA ◽

10.22141/1608-1706.4.22.2021.239709 ◽

2021 ◽

Vol 22 (4) ◽

pp. 46-51

Author(s):

I.I. Trufanov

Keyword(s):

Surgical Treatment ◽

Road Traffic ◽

Platelet Rich Plasma ◽

Clinical Effectiveness ◽

Postoperative Management ◽

Main Group ◽

The Body ◽

Social Adaptation ◽

Acetabular Fractures ◽

Control Group

Background. Acetabular fractures are severe intra-articular injuries that require anatomical reposition and early function, but they are often complicated by degenerative changes in both the acetabulum and the femoral head, leading to the development of post-traumatic coxarthrosis. The purpose was to study the clinical effectiveness of surgical treatment and postoperative management of patients using autologous platelet-rich plasma in patients with fractures of the acetabulum. Materials and methods. Twenty-eight patients with acetabular fractures of various degrees were operated at the City Emergency Hospital of Zaporizhzhia and the Municipal Clinical Hospital No 9 from 2017 to 2019. Gender composition: 22 men (78.57 %), 6 women (21.43 %), average age 46.64 ± 2.21 years, with a 95% confidence interval 42.31–50.96. Nineteen victims (67.86 %) had road traffic injuries, 7 (25 %) domestic injuries, and 2 (7.14 %) had industrial injuries. Results. In the main group of patients treated with platelet-rich plasma, radiologically visible adhesion of the injured area after 8 weeks occurred in 10 people (83.4 %). In one person (8.3 %), the adhesion occurred after 12, and in another (8.3 %) — 16 weeks after surgery. In the control group, adhesions at 8th week were registered in 14 patients (60.87 %), in 8 (34.78 %) — at 16th week. Given the general recovery of the body in the main group, the activation and social adaptation of patients occurred 2–3 weeks earlier. The assessment was performed radiologically and by the criteria of functional recovery.

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