scholarly journals Comparison of The Variability of Small Extracellular Vesicles Derived From Human Liver Cancer Tissues And Cultured From The Tissue Explants Based On A Simple Enrichment Method: Yield, Purity And Molecular Markers

2021 ◽  
Author(s):  
Jie Chen ◽  
Zhigang Jiao ◽  
Jianwen Mo ◽  
Defa Huang ◽  
Zhengzhe Li ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Human Liver ◽  
Extracellular Vesicle ◽  
Interstitial Space ◽  
Cancer Tissue ◽  
Pathological State ◽  
Isolation And Purification ◽  
Tissue Explants ◽  
Human Liver Cancer ◽  
Cancer Tissues

Abstract A potential of using small extracellular vesicle (sEV) for diagnostic and therapeutic purposes has attracted great interest. Some sEVs, directly derived from various tissues, can reflect the physiological and pathological state of the organism. Currently, there are two sources for obtaining tissue-derived sEV: the interstitial space of tissues and cultivation of tissue explants. The former was obtained from tissues that were digested with enzymes and the latter was released by tissue explants. In the present study, we established a new method for the isolation and purification of sEVs from the interstitial space of human liver cancer tissue. Tissues-derived sEVs (TdsEVs) isolated by this method and cultured explants-derived sEVs (cedsEVs) were both characterized by nano-flow cytometry (NanoFCM) for concentration, size distribution and purity. These vesicles were identified and compared by transmission electron microscopy and western blot. TdsEVs have a larger particle size, higher particle concentration and purity than cedsEVs. This study establishes a simple sEV isolation and purification protocol and provides a basis for selection and reference for researches of tdsEVs and cedsEVs.

scholarly journals Comparison of the Variability of Small Extracellular Vesicles Derived from Human Liver Cancer Tissues and Cultured from the Tissue Explants Based on a Simple Enrichment Method

2021 ◽  
Author(s):  
Jie Chen ◽  
Zhigang Jiao ◽  
Jianwen Mo ◽  
Defa Huang ◽  
Zhengzhe Li ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Extracellular Vesicles ◽  
Pathological State ◽  
Enrichment Method ◽  
Isolation And Purification ◽  
Human Liver Cancer ◽  
Cancer Tissues ◽  
Potential Use ◽  
Tissue Digestion

AbstractA potential use of small extracellular vesicles (sEVs) for diagnostic and therapeutic purposes has recently generated a great interest. sEVs, when purified directly from various tissues with proper procedures, can reflect the physiological and pathological state of the organism. However, the quality of sEV is affected by many factors during isolation, including separation of sEV from cell and tissues debris, the use of enzymes for tissue digestion, and the storage state of tissues. In the present study, we established an assay for the isolation and purification of liver cancer tissues-derived sEVs (tdsEVs) and cultured explants-derived sEVs (cedsEVs) by comparing the quality of sEVs derived from different concentration of digestion enzyme and incubation time. The nano-flow cytometry (NanoFCM) showed that the isolated tdsEVs by our method are purer than those obtained from differential ultracentrifugation. Our study thus establishes a simple and effective approach for isolation of high-quality sEVs that can be used for analysis of their constituents. Graphical abstract

Synchrotron refractive-index microradiography of human liver cancer tissue

2005 ◽  
Vol 50 (22) ◽  
pp. 2657-2661
Author(s):  
Yongpeng Tong ◽  
Guilin Zhang ◽  
Yan Li ◽  
Yeukuang Hwu ◽  
Wenli Tsai ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liver Cancer ◽  
Human Liver ◽  
Cancer Tissue ◽  
Human Liver Cancer

scholarly journals Study on 1,25-dihydroxyvitamin D3 receptor in human liver cancer tissue.

Kanzo ◽  
1988 ◽  
Vol 29 (12) ◽  
pp. 1612-1618 ◽  
Author(s):  
Yuuji SAKAI ◽  
Yoshihiro FUKUDA ◽  
Itsuo YAMAMOTO ◽  
Shigeharu DOKOH ◽  
Hiroo IMURA
Keyword(s):  
Liver Cancer ◽  
Human Liver ◽  
Cancer Tissue ◽  
D3 Receptor ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Human Liver Cancer

Precise Electrical Detection of Curcumin Cytotoxicity in Human Liver Cancer Cells

2021 ◽  
Author(s):  
Novi Angeline ◽  
Sung-Sik Choo ◽  
Cheol-Hwi Kim ◽  
Suk Ho Bhang ◽  
Tae-Hyung Kim
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Electrical Detection ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

scholarly journals Inhibition of human liver cancer cell growth by evodiamine involves apoptosis and deactivation of PI3K/AKT pathway

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Jia Jia ◽  
Xigang Kang ◽  
Yanfang Liu ◽  
Jianwei Zhang
Keyword(s):  
Liver Cancer ◽  
Cancer Cell ◽  
Human Liver ◽  
Akt Pathway ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Liver Cancer Cell ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer ◽  
Induction Of Apoptosis

Abstract Evodiamine is an active alkaloid member found in Traditional Chinese Herb (TCH) Evodia rutaecarpa. It has been reported to exhibit remarkable biological and medicinal activities including anticancer and anti-inflammatory. This study was designed to investigate the anticancer effects of evodiamine against human liver cancer and evaluate its effects on cell migration, cell invasion, cellular apoptosis and PI3K/AKT pathway. The results showed that evodiamine exhibits potent antiproliferative effects against two human liver cancer cell lines (HepG2 and PLHC-1) with an IC50 of 20 µM. Nonetheless, the cytotoxic effects of evodiamine were comparatively low against the normal cells as evident from the IC50 of 100 μM. The growth inhibitory effects of evodiamine were found to be due to the induction of apoptosis as revealed by the DAPI, AO/EB and annexin V/PI staining assays. The induction of apoptosis was also associated with upregulation of Bax and downregulation of Bcl-2 expression in a concentration dependent manner. The wound healing and transwell assay revealed that evodiamine caused a significant decline in the migration and invasion of the HepG2 and PLHC-1 cells. Investigation of the effects of evodiamine on the PI3K/AKT signalling revealed that evodiamine inhibited the phosphorylation of PI3K and AKT proteins. Taken together, the results showed that evodiamine inhibits the growth of human liver cancer via induction of apoptosis and deactivation of PI3K/AKT pathway. The results point towards the therapeutic potential of evodiamine in the treatment of liver cancer.

scholarly journals Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using Hepg2 Cell Line

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4593
Author(s):  
Deepthi Venkatachalapathy ◽  
Chandan Shivamallu ◽  
Shashanka Prasad ◽  
Gopenath Thangaraj Saradha ◽  
Parthiban Rudrapathy ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Vitis Vinifera ◽  
Bioactive Compounds ◽  
Camellia Sinensis ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Withania Somnifera ◽  
Antioxidant Properties ◽  
Annexin V ◽  
Human Liver Cancer

The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells.

scholarly journals Aberrant miRNA expression response to UV irradiation in human liver cancer cells

2014 ◽  
Vol 9 (3) ◽  
pp. 904-910 ◽  
Author(s):  
GAOFENG LIANG ◽  
GUANGDA LI ◽  
YANYAN WANG ◽  
WANJUN LEI ◽  
ZHONGDANG XIAO
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Uv Irradiation ◽  
Mirna Expression ◽  
Human Liver ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Expression Response ◽  
Human Liver Cancer Cells
Sign in / Sign up

Export Citation Format

Share Document