scholarly journals Custodiol HTK Versus Plegisol: in-Vitro Comparison With The Use of Immature (H9C2) and Mature (HCM) Cardiomyocytes Cultures

2021 ◽  
Author(s):  
Rafał Nowicki ◽  
Mikołaj Berezowski ◽  
Julita Kulbacka ◽  
Katarzyna Bieżuńska-Kusiak ◽  
Marek Jasiński ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cultured Cells ◽  
Cytotoxicity Test ◽  
Oxidative Stress Markers ◽  
Human Cardiomyocytes ◽  
Stress Markers ◽  
Crystalloid Solutions ◽  
Vitro Model ◽  
Cardioplegic Solutions

Abstract Background: Although cardioplegia is used since the ‘70s of the last century, debate on cardioprotection during cardio-surgical procedures is still actual. The selection of a particular method depends mainly on the preferences and experience of a specific center or even surgeon. Crystalloid cardioplegia is an aqueous ion solution similar to intracellular (Custodiol HTK) or extracellular (Plegisol) fluid. Numerous publications compare different types of cardioplegic solutions, but only a few used cultured cells in laboratory conditions. Methods: In this study, the authors compare two crystalloid solutions using an in-vitro model simulating cardioplegic arrest. The efficacy of myocardial protection during ischemia was investigated with susceptible indicators like the appearance of the deleterious effect of reactive oxygen species and oxidative stress markers. Incubated human cardiomyocytes and rat cardiomyoblasts H9C2 in cardioplegia for 4h were examined for expression of oxidative stress markers (MnSOD, iNOS, HSP27), cardioplegic solutions cytotoxicity, and peroxidation damage of the cell’s lipids and proteins. All tests were performed after 0.5h, 1h, 2h, and 4h in identical physical and biological conditions, which is difficult to achieve in clinical trials. Results: The tests performed on matured cells of human cardiomyocytes showed the superiority of Custodiol HTK. Differences between solutions on immature cells H9C2 were not relevant. Both Plegisol and Custodiol HTK produced a similar expression of MnSOD and iNOS. There was no significant advantage of Custodiol over Plegisol in the cytotoxicity test. However, Custodiol induced a higher level of lipid peroxidation. Conclusions: Considering proceeded examinations on cultured cardiomyocytes, Custodiol HTK appears to be safer than Plegisol.

Measurement of Oxidative Stress Markers In Vitro Using Commercially Available Kits

2020 ◽  
pp. 39-60 ◽  
Author(s):  
Bryan Gardiner ◽  
Julie A. Dougherty ◽  
Devasena Ponnalagu ◽  
Harpreet Singh ◽  
Mark Angelos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Stress Markers ◽  
Stress Markers

In vitro effect of vanadyl sulfate on cultured primary astrocytes: cell viability and oxidative stress markers.

2020 ◽  
Vol 40 (6) ◽  
pp. 737-747
Author(s):  
Agnieszka Ścibior ◽  
Konrad A. Szychowski ◽  
Iwona Zwolak ◽  
Klaudia Dachowska ◽  
Jan Gmiński
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Vanadyl Sulfate ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Primary Astrocytes ◽  
Vitro Effect ◽  
And Oxidative Stress

Has seasonality an effect on oxidative stress markers and trace elements levels in patients who undergo in vitro fertilization treatment?

2019 ◽  
Vol 493 ◽  
pp. S628
Author(s):  
V. Castañón Bernardo ◽  
C. Barneo Caragol ◽  
S. Rodríguez González ◽  
I. Vega Naredo ◽  
L. Sánchez Castro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Trace Elements ◽  
In Vitro Fertilization ◽  
Oxidative Stress Markers ◽  
Vitro Fertilization ◽  
Stress Markers

Ochratoxin A presence in Cabernet Sauvignon wine changes antioxidant activity in vitro and oxidative stress markers in vivo

2020 ◽  
Vol 37 (10) ◽  
pp. 1755-1764
Author(s):  
Luana Schmidt ◽  
Natália de Vargas Heck ◽  
Ingrid Ferreira ◽  
Gabriela Göethel ◽  
Sabrina Somacal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Ochratoxin A ◽  
Cabernet Sauvignon ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

Ferulic acid attenuates osteoporosis induced by glucocorticoid through regulating the GSK-3β/Lrp-5/ERK signalling pathways

2021 ◽  
Author(s):  
Wei Zhou ◽  
Bo Chen ◽  
Jingbo Shang ◽  
Renbo Li
Keyword(s):  
Oxidative Stress ◽  
Ferulic Acid ◽  
Saline Control ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Expression Ratio ◽  
Stress Markers ◽  
Osteogenic Markers ◽  
Gsk 3Β

Abstract Objective To evaluate in-vivo and in-vitro effects of ferulic acid (FA) on glucocorticoid-induced osteoarthritis (GIO) to establish its possible underlying mechanisms. Methods The effects of FA on cell proliferation, cell viability (MTT assay), ALP activity, and mineralization assay, and oxidative stress markers (ROS, SOD, GSH LDH and MDA levels) were investigated by MC3T3-E1 cell line. Wistar rats received standard saline (control group) or dexamethasone (GC, 2 mg−1 kg) or DEX+FA (50 and 100 mg−1 kg) orally for 8 weeks. Bone density, micro-architecture, bio-mechanics, bone turnover markers and histo-morphology were determined. The expression of OPG, RANKL, osteogenic markers, and other signalling proteins was assessed employing quantitative RT-PCR and Western blotting. Results The findings indicated the elevation of ALP mRNA expressions, osteogenic markers (Runx-2, OSX, Col-I, and OSN), and the β-Catenin, Lrp-5 and GSK-3β protein expressions. FA showed the potential to increase MC3T3-E1 cell differentiation, proliferation, and mineralization. FA increased oxidative stress markers (SOD, MDA, and GSH) while decreasing ROS levels and lactate dehydrogenase release in GIO rats. The OPG/RANKL mRNA expression ratio was increased by FA, followed by improved GSK-3β and ERK phosphorylation with enhanced mRNA expressions of Lrp-5 and β-catenin. Conclusion These findings showed that FA improved osteoblasts proliferation with oxidative stress suppression by controlling the Lrp-5/GSK-3β/ERK pathway in GIO, demonstrating the potential pathways involved in the mechanism of actions of FA in GIO therapy.

scholarly journals Irvingia gabonensis Seed Extract: An Effective Attenuator of Doxorubicin-Mediated Cardiotoxicity in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Olufunke Olorundare ◽  
Adejuwon Adeneye ◽  
Akinyele Akinsola ◽  
Phillip Kolo ◽  
Olalekan Agede ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Wistar Rats ◽  
Troponin I ◽  
Disease Risk ◽  
Seed Extract ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Irvingia Gabonensis

Cardiotoxicity as an off-target effect of doxorubicin therapy is a major limiting factor for its clinical use as a choice cytotoxic agent. Seeds of Irvingia gabonensis have been reported to possess both nutritional and medicinal values which include antidiabetic, weight losing, antihyperlipidemic, and antioxidative effects. Protective effects of Irvingia gabonensis ethanol seed extract (IGESE) was investigated in doxorubicin (DOX)-mediated cardiotoxicity induced with single intraperitoneal injection of 15 mg/kg of DOX following the oral pretreatments of Wistar rats with 100-400 mg/kg/day of IGESE for 10 days, using serum cardiac enzyme markers (cardiac troponin I (cTI) and lactate dehydrogenase (LDH)), cardiac tissue oxidative stress markers (catalase (CAT), malonyldialdehyde (MDA), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), and reduced glutathione (GSH)), and cardiac histopathology endpoints. In addition, both qualitative and quantitative analyses to determine IGESE’s secondary metabolites profile and its in vitro antioxidant activities were also conducted. Results revealed that serum cTnI and LDH were significantly elevated by the DOX treatment. Similarly, activities of tissue SOD, CAT, GST, and GSH levels were profoundly reduced, while GPx activity and MDA levels were profoundly increased by DOX treatment. These biochemical changes were associated with microthrombi formation in the DOX-treated cardiac tissues on histological examination. However, oral pretreatments with 100-400 mg/kg/day of IGESE dissolved in 5% DMSO in distilled water significantly attenuated increases in the serum cTnI and LDH, prevented significant alterations in the serum lipid profile and the tissue activities and levels of oxidative stress markers while improving cardiovascular disease risk indices and DOX-induced histopathological lesions. The in vitro antioxidant studies showed IGESE to have good antioxidant profile and contained 56 major secondary metabolites prominent among which are γ-sitosterol, Phytol, neophytadiene, stigmasterol, vitamin E, hexadecanoic acid and its ethyl ester, Phytyl palmitate, campesterol, lupeol, and squalene. Overall, both the in vitro and in vivo findings indicate that IGESE may be a promising prophylactic cardioprotective agent against DOX-induced cardiotoxicity, at least in part mediated via IGESE’s antioxidant and free radical scavenging and antithrombotic mechanisms.

scholarly journals Berries and oxidative stress markers: an overview of human intervention studies

2015 ◽  
Vol 6 (9) ◽  
pp. 2890-2917 ◽  
Author(s):  
Cristian Del Bo’ ◽  
Daniela Martini ◽  
Marisa Porrini ◽  
Dorothy Klimis-Zacas ◽  
Patrizia Riso
Keyword(s):  
Oxidative Stress ◽  
Intervention Studies ◽  
Human Intervention ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Intervention Trials ◽  
And Oxidative Stress

Severalin vitroandin vivostudies have demonstrated that polyphenol-rich berries may counteract oxidative stress. In this review, we summarized the main finding from human intervention trials on the role of berries in the modulation of markers of oxidative lipid, protein and DNA damage.

scholarly journals Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Zhang Yifan ◽  
Ning Benxiang ◽  
Xu Zheng ◽  
Xu Luwei ◽  
Zhou Liuhua ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Kidney Injury ◽  
Oxidative Stress Markers ◽  
Stress Injury ◽  
Stress Markers ◽  
Oxidative Stress Injury ◽  
Calcium Crystals ◽  
And Oxidative Stress

Objective. To investigate the role of inflammatory reactions and oxidative stress injury in the mechanisms of ceftriaxone calcium crystal-induced acute kidney injury (AKI) both in vivo and in vitro. Methods. Male Sprague Dawley rats were randomly divided into five groups of ten each according to different concentrations of ceftriaxone and calcium. Based on the levels of serum creatinine (Scr) and blood urea nitrogen (BUN), the AKI group was chosen for the subsequent experiments. Kidney histological examination and immunohistochemistry were performed. The expression of NLRP3 and IL-1β protein and the concentrations of oxidative stress markers such as ROS, MDA, and H2O2 in kidney tissues were estimated. In parallel, HK-2 human renal proximal tubule cells were exposed to ceftriaxone calcium crystals. The mRNA expression levels of NLRP3 and IL-1β and the concentrations of oxidative stress markers were evaluated. Finally, cell viability and rat survival were also assessed. Results. The results showed that significantly increased Scr and BUN levels, consistent with morphological changes and kidney stones, were found in the rats that received the highest concentration of ceftriaxone (1000 mg/kg) combined with calcium (800 mg/kg). The activation of the NLRP3 inflammasome axis and the marked elevation of MDA, H2O2, and ROS levels were observed both in vivo and in vitro. High expression of Nrf2, HO-1, and NQO1 was also documented. In addition, cell apoptosis and rat mortality were promoted by ceftriaxone calcium crystals. Conclusions. Notably, we found that ceftriaxone-induced urolithiasis was associated with a high risk of AKI and NLRP3-mediated inflammasome and oxidative stress injury were of major importance in the pathogenesis.

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