scholarly journals Design and Synthesis of a Siderophore-Based Fluorescent Probe and Its Application in Selective Detection of Aeromonas

2021 ◽  
Author(s):  
Javier Cisneros-Sureda ◽  
Diego Rey-Varela ◽  
Jaime Rodríguez ◽  
Miguel Balado ◽  
Manuel L. Lemos ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Iron Uptake ◽  
Pathogenic Bacteria ◽  
Click Reaction ◽  
Growth Promotion ◽  
Fish Farming ◽  
Uptake Mechanism ◽  
Design And Synthesis ◽  
Selective Labelling ◽  
Sulforhodamine B

Abstract Amonabactins, the siderophores produced by some pathogenic bacteria belonging to Aeromonas genus, can be used for the preparation of conjugates that can be imported into the cell using their specific transport machinery. Herein, we report the design and synthesis of a new amonabactin-based fluorescent probe by conjugation of the appropiate amonabactin analogue to sulforhodamine B (AMB-SRB) using a thiol-maleimide click reaction. Growth promotion assays and fluorescence microscopy studies demonstrated that AMB-SRB fluorescent probe was able to label the fish pathogenic bacterium A. salmonicida subsp. salmonicida through its outer membrane transport (OMT) protein FstC. The labelling of other Aeromonas species such as the human pathogenic A. hydrophila, indicates that this probe can be a very useful molecular tool for studying the amonabactin-dependent iron uptake mechanism. Furthermore, the selective labelling of A. salmonicida and other Aeromonas species in presence of other fish pathogenic bacteria, suggest the potential application of this probe for detection of Aeromonas in water and other fish farming samples through fluorescence assays.

scholarly journals Design and Synthesis of (2-oxo-1,2-Dihydroquinolin-4-yl)-1,2,3-triazole Derivatives via Click Reaction: Potential Apoptotic Antiproliferative Agents

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6798
Author(s):  
Essmat M. El-Sheref ◽  
Mohammed A. I. Elbastawesy ◽  
Alan B. Brown ◽  
Ahmed M. Shawky ◽  
Hesham A. M. Gomaa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Click Reaction ◽  
Annexin V ◽  
Antiproliferative Agents ◽  
Design And Synthesis ◽  
Cycle Arrest ◽  
M Phase ◽  
Reaction Potential ◽  
Apoptotic Activity ◽  
Mcf 7

A mild and versatile method based on Cu-catalyzed [2+3] cycloaddition (Huisgen-Meldal-Sharpless reaction) was developed to tether 3,3’-((4-(prop-2-yn-1-yloxy)phenyl)methylene)bis(4-hydroxyquinolin-2(1H)-ones) with 4-azido-2-quinolones in good yields. This methodology allowed attaching three quinolone molecules via a triazole linker with the proposed mechanism. The products are interesting precursors for their anti-proliferative activity. Compound 8g was the most active one, achieving IC50 = 1.2 ± 0.2 µM and 1.4 ± 0.2 µM against MCF-7 and Panc-1 cell lines, respectively. Moreover, cell cycle analysis of cells MCF-7 treated with 8g showed cell cycle arrest at the G2/M phase (supported by Caspase-3,8,9, Cytochrome C, BAX, and Bcl-2 studies). Additionally, significant pro-apoptotic activity is indicated by annexin V-FITC staining.

scholarly journals Iron uptake mechanisms of pathogenic bacteria

1993 ◽  
Vol 12 (4) ◽  
pp. 325-348 ◽  
Author(s):  
Karl G. Wooldridge ◽  
Peter H. Williams
Keyword(s):  
Iron Uptake ◽  
Pathogenic Bacteria ◽  
Uptake Mechanisms

A new mechanism for membrane iron transport in Pseudomonas aeruginosa

2002 ◽  
Vol 30 (4) ◽  
pp. 702-705 ◽  
Author(s):  
I.J. Schalk ◽  
M. A. Abdallah ◽  
F. Pattus
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Outer Membrane ◽  
Iron Uptake ◽  
Iron Transport ◽  
Membrane Receptor ◽  
Gram Negative Bacteria ◽  
Outer Membrane Receptor ◽  
Uptake Mechanism ◽  
Atp Binding Cassette Transporter ◽  
Biophysical Studies

Various biochemical and biophysical studies have demonstrated the existence of a novel iron-uptake mechanism in Pseudomonas aeruginosa, different from that generally described for ferrichrome and ferric-enterobactin in Escherichia coli. This new iron-uptake mechanism involves all the proteins generally reported to be involved in the uptake of ferric-siderophore complexes in Gram-negative bacteria (i.e. the outer membrane receptor, periplasmic binding protein and ATP-binding-cassette transporter), but differs in the behaviour of the siderophore. One of the key features of this process is the binding of iron-free pyoverdin to the outer membrane receptor FpvA in conditions of iron deficiency.

scholarly journals Insights into the chemistry of the amphibactin–metal (M3+) interaction and its role in antibiotic resistance

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Vidya Kaipanchery ◽  
Anamika Sharma ◽  
Fernando Albericio ◽  
Beatriz G. de la Torre
Keyword(s):  
Metal Ions ◽  
Iron Uptake ◽  
Pathogenic Bacteria ◽  
Iron Acquisition ◽  
Transition Metal Ions ◽  
Low Molecular Weight ◽  
Acquisition Strategies ◽  
Iron Deficient ◽  
Fe Uptake ◽  
Vibrio Genus

AbstractWe have studied the diversity and specificity of interactions of amphibactin produced by Vibrio genus bacterium (Vibrio sp. HC0601C5) with iron and various metal ions in + 3 oxidation state in an octahedral (Oh) environment. To survive in the iron-deficient environment of their host, pathogenic bacteria have devised various efficient iron acquisition strategies. One such strategy involves the production of low molecular weight peptides called siderophores, which have a strong affinity and specificity to chelate Fe3+ and can thus facilitate uptake of this metal in order to ensure iron requirements. The Fe uptake by amphibactin and the release of iron inside the cell have been studied. Comparison of the interaction of different transition metal ions (M3+) with amphibactin has been studied and it reveals that Co and Ga form stable complexes with this siderophore. The competition of Co and Ga with Fe impedes iron uptake by bacteria, thereby preventing infection.

scholarly journals Is there a strategy I iron uptake mechanism in maize?

2018 ◽  
Vol 13 (4) ◽  
pp. e1161877 ◽  
Author(s):  
Suzhen Li ◽  
Xiaojin Zhou ◽  
Jingtang Chen ◽  
Rumei Chen
Keyword(s):  
Iron Uptake ◽  
Uptake Mechanism ◽  
Strategy I

scholarly journals Niobium Uptake and Release by Bacterial Ferric Ion Binding Protein

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Yanbo Shi ◽  
Ian Harvey ◽  
Dominic Campopiano ◽  
Peter J. Sadler
Keyword(s):  
Iron Uptake ◽  
Pathogenic Bacteria ◽  
Ion Binding ◽  
Ferric Ion ◽  
Gram Negative Bacteria ◽  
Icp Oes ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Binding Cleft ◽  
X Ray Absorption

Ferric ion binding proteins (Fbps) transportFeIIIacross the periplasm and are vital for the virulence of many Gram negative bacteria. Iron(III) is tightly bound in a hinged binding cleft with octahedral coordination geometry involving binding to protein side chains (including tyrosinate residues) together with a synergistic anion such as phosphate. Niobium compounds are of interest for their potential biological activity, which has been little explored. We have studied the binding of cyclopentadienyl and nitrilotriacetatoNbVcomplexes to the Fbp fromNeisseria gonorrhoeaeby UV-vis spectroscopy, chromatography, ICP-OES, mass spectrometry, and Nb K-edge X-ray absorption spectroscopy. These data suggest thatNbVbinds strongly to Fbp and that a dinuclearNbVcentre can be readily accommodated in the interdomain binding cleft. The possibility of designing niobium-based antibiotics which block iron uptake by pathogenic bacteria is discussed.

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