scholarly journals A Rapid Microwave Mediated Polyethyleneglycol Embedding Method Showing Retention of Intracellular Specialized Metabolites in Leaves of Cinnamomum Tamala (Buch-Ham.) T. Nees & Nees

2020 ◽  
Author(s):  
Sulagna Saha ◽  
Raktim Bhattacharya ◽  
Adinpunya Mitra
Keyword(s):  
Traditional Method ◽  
Microwave Oven ◽  
Embedding Method ◽  
Specialized Metabolites ◽  
Straightforward Method ◽  
Cinnamomum Tamala ◽  
Embedded Methods ◽  
Embedding Medium ◽  
Heating Medium

Abstract Polyethyleneglycol (PEG) is considered one of the most effective substitutions for paraffin in plant histochemistry as an embedding medium. A rapid and straightforward method of PEG embedding has been developed that resulted in a significant reduction of infiltration time than the traditional method of PEG embedding. The material used for PEG embedding was leaves of Cinnamomum tamala, a member of Lauraceae. Samples were put successively in aqueous solutions of PEG 6000 with increasing concentration for infiltration. A microwave oven was used as a mode of heating medium. The infiltration was completed within 2 h. After the completion of infiltration, the samples were embedded in PEG and solidified. Compared with the existing methods available for PEG infiltration and embedding, this microwave mediated PEG embedding method saves significant time; this also saves the tissue from long-term heat-induced damage. Retention of intracellular metabolites, which was not possible in earlier PEG embedded methods, has also demonstrated in the tissue.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

A Comparative Evaluation on effective perception of Aamottpati Siddhant to 3rd BAMS students by traditional method (tree diagram) versus Audio-Visual method

2017 ◽  
Vol 1 (4) ◽  
Author(s):  
Sundarsingh K. Danga ◽  
Shubhangi Danga
Keyword(s):  
Significant Role ◽  
Traditional Method ◽  
Comparative Evaluation ◽  
Teaching Method ◽  
Visual Method ◽  
Tree Diagram ◽  
Method Student ◽  
Long Term Impact ◽  
Factual Recall

Effective teaching plays very significant role in acquiring knowledge with long term impact and remembrance. In teaching process many times we focus on factual recall rather than reasoning skills or understanding something better where learners are not actively involved. In this study students of 3rd phase BAMS were taught Aamottpatti Siddhant by Traditional Method (Tree Diagram) versus Audio-Visual Method. Student were familiar of traditional method of teaching however after introducing audio visual method, interest in lecture was increased in students. Audio visual method can be associated with the traditional type of teaching method. Teaching by Audio visual method is interesting to student as well as teachers.

Bio/non-bio interfaces: A straightforward method for obtaining long term PDMS/muscle cell biohybrid constructs

2013 ◽  
Vol 105 ◽  
pp. 144-151 ◽  
Author(s):  
Giada Graziana Genchi ◽  
Gianni Ciofani ◽  
Ioannis Liakos ◽  
Leonardo Ricotti ◽  
Luca Ceseracciu ◽  
...  
Keyword(s):  
Muscle Cell ◽  
Straightforward Method

scholarly journals Functional Assessment of the Stomatognathic System, after the Treatment of Edentulous Patients, with Different Methods of Establishing the Centric Relation

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Aleksandra Nitecka-Buchta ◽  
Thomas Proba ◽  
Paulina Proba ◽  
Kamil Stefański ◽  
Stefan Baron
Keyword(s):  
Functional Assessment ◽  
Traditional Method ◽  
Complete Dentures ◽  
Subjective Experiences ◽  
Stomatognathic System ◽  
Centric Relation ◽  
Edentulous Patients ◽  
Tracing Method ◽  
Gothic Arch

The study compares subjective experiences of patients, wearing complete dentures. Two different methods of determining a centric relation were performed: the traditional method using wax occlusal rims and the Gerber method, based on gothic arch tracings. The success rate of establishing a centric relation in both methods was evaluated (rentgenodiagnostics). The influence of the method used to obtain the centric relation on patients’ stomatognathic system (condyle centralization, pain) was also evaluated. Better results were achieved in gothic arch tracing method. Before every prosthetic treatment of edentulous patients, a functional analysis of the TMJ is necessary. The lack of centric relation, in a long term adaptation patients, does not lead to TMD symptoms. This trial is registered with NCT03343015.

Design of green microorganized systems for decontamination of ecotoxicants

2008 ◽  
Vol 80 (7) ◽  
pp. 1381-1397 ◽  
Author(s):  
Anatoliy F. Popov
Keyword(s):  
Organophosphorus Compounds ◽  
The Novel ◽  
Basic Principles ◽  
Micellar Phase ◽  
Straightforward Method ◽  
Cationic Detergents ◽  
Reactions With Nucleophiles ◽  
Derivatives Of ◽  
Low Solubility

Due to population upsurge, pesticides (derivatives of organophosphorus acids included) find widespread use in agriculture. The toxicity and long-term environmental hazard of such compounds require detailed studies on decomposition mechanisms of the pesticides and development of efficient, readily available, and inexpensive systems for their decontamination. A simple and straightforward method for the decomposition of organophosphorus compounds involves their reactions with nucleophiles. Studies on the reactivity of "normal" and α-nucleophiles toward electron-deficient centers allowed us to state a number of basic principles on the nature of the α-effect. One of the most important conclusions is: It is unlikely that the structure variations in the known α-nucleophiles will result in higher reactivity than that of hydroxylamine anion. As a practical matter, the essential disadvantages of decomposition of organophosphorus compounds in water and organic solvents are: (i) instability of active nucleophiles and oxidizing agents, (ii) corrosiveness, and (iii) extremely low solubility of organophosphorus compounds in water. These dictate the strategy of further studies, namely, carrying out the reaction in microorganized media. The first research line involves the development of the functional imidazole-based detergents functionalized with α-nucleophilic fragments. A number of efficient detergents were first synthesized. An examination of the nucleophilicity of the functional fragments in water and in the micellar phase and quantitative assessment of the factors responsible for the micellar effects showed unambiguously that the main cause of the observed accelerations is the substrate concentrating in the micellar pseudophase. The second research line consists of studies on the reactivity of versatile oxidative/nucleophilic systems involving H2O2/activator and polyhalide ion organocomplexes in water, aqueous alcohols, and micelles of cationic detergents. The novel sources of "active" halogen are highly competitive both with conventional hazardous chlorine derivatives and "green" systems involving H2O2/activator.

A Change of Traditional Method of Treatment of Partial Thickness Burn with Hydrofibre Dressings

2015 ◽  
Vol 87 (2) ◽  
Author(s):  
Kryspin Mitura ◽  
Jolanta Osłowska ◽  
Alicja Mitura
Keyword(s):  
Traditional Method ◽  
Treatment Time ◽  
Healing Process ◽  
Silver Ions ◽  
Long Term Results ◽  
Burn Wound ◽  
Partial Thickness ◽  
Partial Thickness Burn ◽  
Short And Long Term

AbstractNearly 1% of population is affected with burn trauma annually. Among patients seeking advanced medical care almost 8% require hospital stay. Most burns are superficial and do not cause full thickness damage to the skin. Standard topical treatment of burn wounds with creams and ointments requires frequent dressings’ changes. The healing process is long and expensive. The technological revolution, as occurred in the last decade in the field of dressing care allows us to introduce the use of advanced material solutions. Modern materials allow to shorten the treatment time, reduce the expenses and diminish the patient discomfort leading to good outcome.The study presented a case of four patients treated due to the partial thickness burn wound. Hydrofiber dressing with silver ions were introduced in the treatment. We observed good short and long term results

A Longitudinal Evaluation of Tablet-Based Child Speech Therapy with Apraxia World

2021 ◽  
Vol 14 (1) ◽  
pp. 1-26
Author(s):  
Adam Hair ◽  
Kirrie J. Ballard ◽  
Constantina Markoulli ◽  
Penelope Monroe ◽  
Jacqueline Mckechnie ◽  
...  
Keyword(s):  
Traditional Method ◽  
Speech Therapy ◽  
Treatment Phase ◽  
Evaluation Framework ◽  
Home Practice ◽  
Child Speech ◽  
Received Pronunciation ◽  
Child Engagement ◽  
Over Time

Digital games can make speech therapy exercises more enjoyable for children and increase their motivation during therapy. However, many such games developed to date have not been designed for long-term use. To address this issue, we developed Apraxia World, a speech therapy game specifically intended to be played over extended periods. In this study, we examined pronunciation improvements, child engagement over time, and caregiver and automated pronunciation evaluation accuracy while using our game over a multi-month period. Ten children played Apraxia World at home during two counterbalanced 4-week treatment blocks separated by a 2-week break. In one treatment phase, children received pronunciation feedback from caregivers and in the other treatment phase, utterances were evaluated with an automated framework built into the game. We found that children made therapeutically significant speech improvements while using Apraxia World, and that the game successfully increased engagement during speech therapy practice. Additionally, in offline mispronunciation detection tests, our automated pronunciation evaluation framework outperformed a traditional method based on goodness of pronunciation scoring. Our results suggest that this type of speech therapy game is a valid complement to traditional home practice.

scholarly journals Effects of reverse deployment of cone-shaped vena cava filter on improvements in hemodynamic performance in vena cava

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Ying Chen ◽  
Zaipin Xu ◽  
Xiaoyan Deng ◽  
Shibo Yang ◽  
Wenchang Tan ◽  
...  
Keyword(s):  
Shear Stress ◽  
Shear Rate ◽  
Traditional Method ◽  
Blood Clot ◽  
Animal Experiments ◽  
Vena Cava ◽  
Vena Cava Filters ◽  
Relative Residence Time ◽  
Hemodynamic Performance

Abstract Background Cone-shaped vena cava filters (VCFs) are widely used to treat venous thromboembolism. However, in the long term, the problem of occlusion persists even after the filter is deployed. A previous study hypothesized that the reverse deployment of a cone-shaped VCFs may prevent filter blockage. Methods To explore this hypothesis, a comparative study of the traditional and reverse deployments of VCFs was conducted using a computational fluid dynamics approach. The distribution of wall shear stress (WSS) and shear stress-related parameters were calculated to evaluate the differences in hemodynamic effects between both conditions. In the animal experiment, we reversely deployed a filter in the vena cava of a goat and analyzed the blood clot distribution in the filter. Results The numerical simulation showed that the reverse deployment of a VCF resulted in a slightly higher shear rate on the thrombus, and no reductions in the oscillating shear index (OSI) and relative residence time (RRT) on the vessel wall. Comparing the traditional method with the reversely deployed cases, the shear rate values is 16.49 and 16.48 1/s, respectively; the minimal OSI values are 0.01 and 0.04, respectively; in the vicinity of the VCF, the RRT values are both approximately 5 1/Pa; and the WSS is approximately 0.3 Pa for both cases. Therefore, the reverse deployment of cone-shaped filters is not advantageous when compared with the traditional method in terms of local hemodynamics. However, it is effective in capturing thrombi in the short term, as demonstrated via animal experiments. The reversely deployed cone-shaped filter captured the thrombi at its center in the experiments. Conclusions Thus, the reverse deployment of cone-shaped filters is not advantageous when compared with the traditional method in terms of local hemodynamics. Therefore, we would not suggest the reverse deployment of the cone-shaped filter in the vena cava to prevent a potentially fatal pulmonary embolism.

scholarly journals Dynamic Laser Speckle Technique Sensing Long-Term Changes Caused by Painting Treatments in Restauration of Paintings

2017 ◽  
Author(s):  
Alberto J. Pérez ◽  
Rolando J. González-Peña ◽  
Roberto Braga Jr ◽  
Ángel Perles ◽  
Eva Pérez–Marín ◽  
...  
Keyword(s):  
Traditional Method ◽  
Laser Speckle ◽  
The Other ◽  
Portable Equipment ◽  
Time Rate ◽  
Alternative Approach ◽  
Long Term Changes ◽  
Slow Activity ◽  
Low Activity

Dynamic laser speckle is applied as a reliable sensor of activity in all sort of material. Traditional applications are based on a time rate that is usually higher than 10 frames-per-second (FPS). Even in drying processes, where there is a high activity in the first moments after the painting and a slow activity after some minutes or hours, the process is based on the acquisition of images in a time rate that is the same in both moments of high and low activity. In this work, we present an alternative approach to follow the drying of paint and the other processes related to restauration of paintings that takes long-term to reduce the activity. We illuminated, using three different wavelength lasers, an accelerator (Cyclododecane) and a varnish used in restauration of paintings and monitor them at long-term drying using an alternative fps, comparing the results to the traditional method. The work also presents a way to do the monitoring using a portable equipment. The results present the feasibility to use the portable device and show the improvement in the sensitivity of the dynamic laser speckle to sense long-term process regarding the drying of Cyclododecane and Varnish used in restauration.

scholarly journals Regionalization of maximum, minimum and mean streamflows for the Juruena River basin, Brazil

2020 ◽  
Vol 15 (3) ◽  
pp. 1
Author(s):  
Talisson Sáteles Matos ◽  
Eduardo Morgan Uliana ◽  
Camila Aparecida da Silva Martins ◽  
Luis Miguel Castillo Rapalo
Keyword(s):  
River Basin ◽  
Traditional Method ◽  
Resource Planning ◽  
Drainage Area ◽  
Stream Flows ◽  
Explanatory Variables ◽  
Maximum Minimum ◽  
Linear And Nonlinear Regressions ◽  
Physiographic Parameters

  This study aimed to regionalize maximum (Qmax), minimum (Q95), and long-term mean (Qltm) streamflows in the Juruena River Basin to further water-resource planning and management, especially regarding water-use grant rights, streamflow regulation, and hydraulic designs. To do that, a traditional method was used, which relates the interest streamflows with sub-basin physiographic parameters by linear and nonlinear regressions. In summary, the traditional method was efficient for regionalization of Q95, Qltm, and Qmax streamflows for the Juruena River Basin. Moreover, the explanatory variables able to provide the best results for regionalization of Q95 and Qltm stream flows are drainage area, total watercourse length, and sub-basin mean altitude. For Qmax regionalization, the best results were provided by explanatory variables like drainage area, perimeter, and total watercourse length.

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