scholarly journals Determination of Prostate-Specific Antigen Via The Assembly of a Two-Dimensional Nanoplatform

2021 ◽  
Author(s):  
Junjie Chen ◽  
Xiangqian Li ◽  
Xiaoqi Yu ◽  
Qianming Wang
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Two Dimensional ◽  
Detection Range ◽  
Sensing Platform ◽  
Linear Detection ◽  
Highly Sensitive ◽  
Diverse Application ◽  
Aptamer Probe

Abstract Two-dimensional platforms with favorable features are highly expected for diverse application. In this work, we report a highly sensitive and selective “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection base on cobalt oxyhydroxide nanosheets (CoOOH NSs). CoOOH NSs are employed as the suitable sensing hosts, in which fluorescein amidite-(abbreviated as FAM) labeled aptamer probe (PA) has been adsorbed on nanosheets. Energy transfer between substrate and optical species has switched off the fluorescence of PA. The strong affinity of PA to the target PSA induces the formation of a rigid aptamer structure and the integration with the CoOOH NSs has been drastically affected. The recognition process has been followed by the release of the aptamer probe PA from the nanosheet surface and the green luminescence has been recovered. The dynamic nano-sensor exhibits highly sensitive and accurate analytical performance toward PSA with a linear detection range from 0.1 to 5 nM and a detection limit of 56.1 pM. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.

Highly sensitive nanoparticle-based immunoassays with elemental detection: Application to Prostate-Specific Antigen quantification

2016 ◽  
Vol 85 ◽  
pp. 128-134 ◽  
Author(s):  
Marta Garcia-Cortes ◽  
Jorge Ruiz Encinar ◽  
Jose M. Costa-Fernandez ◽  
Alfredo Sanz-Medel
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Highly Sensitive ◽  
Elemental Detection

Prostate-specific antigen in milk of lactating women

1995 ◽  
Vol 41 (1) ◽  
pp. 54-58 ◽  
Author(s):  
H Yu ◽  
E P Diamandis
Keyword(s):  
Breast Milk ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Normal Breast ◽  
Specific Marker ◽  
Time Resolved ◽  
Lactating Women ◽  
Highly Sensitive ◽  
Mother’S Age ◽  
Total Psa

Abstract Prostate-specific antigen (PSA) is believed to be a highly specific marker for normal and cancerous prostatic tissue. We recently found that 30-40% of breast tumors produce PSA. Other data from our group suggest that normal breast can also produce PSA under conditions of stimulation by steroid hormones. In addition, we detected PSA in amniotic fluid. Here we report the presence of PSA in breast milk of lactating women. PSA concentrations in breast milk were quite variable, ranging from < 0.01 microgram/L in 4 of 38 milks to 350 micrograms/L; the median was 0.47 microgram/L. PSA concentration in breast milk was not correlated with mother's age or the sex of the newborn. It did tend to decrease with increasing time postdelivery, but was still detectable 2 weeks postdelivery. PSA in milk was equally measurable by a highly sensitive PSA assay based on time-resolved fluorometry and by the IMx automated PSA method. As confirmed by Western blot analysis, PSA in milk was present predominantly in its 33-kDa form; the PSA-alpha 1-antichymotrypsin complex (100 kDa) was also present but its concentration was < 25% of total PSA. We conclude that the female breast can produce PSA and that PSA is secreted into the milk during lactation; however, the biological role of PSA in milk is unknown. These and other data presented by our group suggest that PSA, a serine protease, may play a role in control of growth in mammary and other tissues through regulation of growth factors, cytokines, and growth-factor-binding proteins.

An electrochemical sensing platform amplified with a Au@Ag nanoparticle-decorated three-dimensional N-doped graphene aerogel for ultrasensitive determination of baicalein

2020 ◽  
Vol 44 (37) ◽  
pp. 15975-15982
Author(s):  
Xueliang Niu ◽  
Weili Zhang ◽  
Yan Huang ◽  
Likai Wang ◽  
Zhongfang Li ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Electrochemical Sensing ◽  
Ag Nanoparticle ◽  
Graphene Aerogel ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Signal Amplifier ◽  
Doped Graphene ◽  
Sensitive Determination

A novel electrochemical method for highly sensitive determination of baicalein was developed with Au@Ag/3DNGA as signal amplifier.

Highly Sensitive Immunofluorescence Assay of Prostate-Specific Antigen Using Silver Nanoparticles

2020 ◽  
Vol 87 (5) ◽  
pp. 870-876
Author(s):  
I. V. Koktysh ◽  
Ya. I. Melnikova ◽  
O. S. Kulakovich ◽  
A. A. Ramanenka ◽  
S. V. Vaschenko ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Immunofluorescence Assay ◽  
Highly Sensitive

scholarly journals Determination of Non-α1-Antichymotrypsin-complexed Prostate-specific Antigen as an Indirect Measurement of Free Prostate-specific Antigen: Analytical Performance and Diagnostic Accuracy

2003 ◽  
Vol 49 (6) ◽  
pp. 887-894 ◽  
Author(s):  
Sebastian Wesseling ◽  
Carsten Stephan ◽  
Axel Semjonow ◽  
Michael Lein ◽  
Brigitte Brux ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Sensitivity And Specificity ◽  
Specific Antigen ◽  
Roc Curves ◽  
Indirect Measurement ◽  
Free Psa ◽  
Specificity And Sensitivity ◽  
Diagnostic Sensitivity And Specificity ◽  
Total Psa

Abstract Background: A new assay measures prostate-specific antigen (PSA) not complexed to α1-antichymotrypsin (nACT-PSA) after removing PSA complexed to ACT by use of anti-ACT antibodies. We evaluated nACT-PSA and its ratio to total PSA (tPSA) as alternatives to free PSA (fPSA) and its ratio to tPSA in differentiating prostate cancer (PCa) and benign prostatic hyperplasia (BPH) in patients with tPSA of 2–20 μg/L. Methods: PSA in serum of 183 untreated patients with PCa and 132 patients with BPH was measured retrospectively on the chemiluminescence immunoassay analyzer LIAISON® (Byk-Sangtec Diagnostica) with the LIAISON tPSA and LIAISON fPSA assays. The nACT-PSA fraction was determined with a prototype assay measuring the residual PSA after precipitation of ACT-PSA with an ACT-precipitating reagent. Results:nACT-PSA was higher than fPSA in samples with fPSA concentrations <1 μg/L but lower in samples with >1 μg/L fPSA. The median ratios of fPSA/tPSA and of nACT-PSA/tPSA were significantly different between patients with BPH and PCa (19.4% vs 12.2% and 17.4% vs 13.0%, respectively). Within the tPSA ranges tested (2–20, 2–10, and 4–10 μg/L), areas under the ROC curves for the fPSA/tPSA ratios were significantly larger than those for nACT-PSA/tPSA. In the tPSA ranges <10 μg/L, the areas under the ROC curves for fPSA/tPSA were significantly larger than those for tPSA, whereas the areas for nACT-PSA/tPSA were not. At decision limits for 95% sensitivity and specificity, both ratios significantly increased specificity and sensitivity, respectively, compared with tPSA, but the fPSA/tPSA ratio showed higher values. Conclusions: nACT-PSA and its ratio to tPSA provide lower diagnostic sensitivity and specificity than fPSA/tPSA. The fPSA/tPSA ratio represents the state-of-the-art method for differentiating between PCa and BPH.

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