Integrated Analysis of Cecal Microbiome and Metabolome Revealed Different Inflammatory Reponses to Salmonella Enterica Serovar Enteritidis Between Reciprocal Crosses of Chicken

Abstract Background: Salmonella enterica serovar Enteritidis (S. Enteritidis) bacteria can colonize intestinal tract of chicken and transmit to humans, while the intestinal microbiota are resistant to their colonization. Our knowledge of the interplay between host, pathogen and microbiota is scarce, particularly in context of different genetic background of the host, such as the reciprocal cross.Results: Comprehensive analysis of microbiome and metabolome showed that 23.4% of genera and 11.6% of metabolites in the Cross, and 17.2% of genera and 25.1% of metabolites in the Reverse-cross were altered (P<0.05) in response to Salmonella infection. In comparison of the reciprocal crosses, all the co-differential genera and 73.1% of the co-differential metabolites were opposite in alteration trend. Phenylpropanoids and lipids were increased significantly in the Cross and in the Reverse-cross, respectively.Conclusions: A more extensive inflammatory response to S. Enteritidis might be triggered in the Reverse-cross than in the Cross, and the regulatory modes were different. The Reverse-cross upregulated pro- and anti-inflammatory factors simultaneously, while the Cross was aided with the phenylpropanoids produced by intestinal bacteria.

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Effects of starvation and refeeding on the excretion of urinary steroid metabolites in mice with different genetic background

Journal of Chromatography A ◽

10.1016/s0021-9673(01)87527-0 ◽

1998 ◽

Vol 308 ◽

pp. 1-9

Author(s):

M Alsandro

Keyword(s):

Genetic Background ◽

Different Genetic Background ◽

Urinary Steroid

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Comprehensive Analysis of Transcriptome and Metabolome Reveals the Flavonoid Metabolic Pathway Is Associated with Fruit Peel Coloration of Melon

Molecules ◽

10.3390/molecules26092830 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2830

Author(s):

Aiai Zhang ◽

Jing Zheng ◽

Xuemiao Chen ◽

Xueyin Shi ◽

Huaisong Wang ◽

...

Keyword(s):

Molecular Mechanisms ◽

Comprehensive Analysis ◽

Differentially Expressed ◽

Integrated Analysis ◽

Fruit Peel ◽

External Quality ◽

Color Formation ◽

Peel Color ◽

Dark Green

The peel color is an important external quality of melon fruit. To explore the mechanisms of melon peel color formation, we performed an integrated analysis of transcriptome and metabolome with three different fruit peel samples (grey-green ‘W’, dark-green ‘B’, and yellow ‘H’). A total of 40 differentially expressed flavonoids were identified. Integrated transcriptomic and metabolomic analyses revealed that flavonoid biosynthesis was associated with the fruit peel coloration of melon. Twelve differentially expressed genes regulated flavonoids synthesis. Among them, nine (two 4CL, F3H, three F3′H, IFS, FNS, and FLS) up-regulated genes were involved in the accumulation of flavones, flavanones, flavonols, and isoflavones, and three (2 ANS and UFGT) down-regulated genes were involved in the accumulation of anthocyanins. This study laid a foundation to understand the molecular mechanisms of melon peel coloration by exploring valuable genes and metabolites.

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Identification and Characterization of Novel Maize Mirnas Involved in Different Genetic Background

International Journal of Biological Sciences ◽

10.7150/ijbs.11619 ◽

2015 ◽

Vol 11 (7) ◽

pp. 781-793 ◽

Cited By ~ 10

Author(s):

Lei Sheng ◽

Wenbo Chai ◽

Xuefeng Gong ◽

Lingyan Zhou ◽

Ronghao Cai ◽

...

Keyword(s):

Genetic Background ◽

Different Genetic Background ◽

Identification And Characterization

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Functionality of cryopreserved juvenile ovaries from mutant mice in different genetic background strains after allotransplantation

Cryobiology ◽

10.1016/j.cryobiol.2009.10.003 ◽

2010 ◽

Vol 60 (2) ◽

pp. 129-137 ◽

Cited By ~ 4

Author(s):

Kuo-Yu Huang ◽

Suzanna A. de Groot ◽

Henri Woelders ◽

Gijsbertus T.J. van der Horst ◽

Axel P.N. Themmen ◽

...

Keyword(s):

Genetic Background ◽

Mutant Mice ◽

Different Genetic Background

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Varying susceptibility to myocardial infarction among C57BL/6 mice of different genetic background

Journal of Molecular and Cellular Cardiology ◽

10.1016/s0022-2828(03)00082-8 ◽

2003 ◽

Vol 35 (6) ◽

pp. 705-708 ◽

Cited By ~ 23

Author(s):

D Gorog

Keyword(s):

Myocardial Infarction ◽

Genetic Background ◽

Different Genetic Background

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Phenotypic and clonal stability of antigen-inexperienced memory-like T cells across the genetic background, hygienic status, and aging

10.1101/2020.09.01.277004 ◽

2020 ◽

Author(s):

Alena Moudra ◽

Veronika Niederlova ◽

Jiri Novotny ◽

Lucie Schmiedova ◽

Jan Kubovciak ◽

...

Keyword(s):

Gene Expression ◽

T Cells ◽

Genetic Background ◽

Expression Profiles ◽

Bacterial Colonization ◽

Gene Expression Profiles ◽

Inbred Strains ◽

Microbial Colonization ◽

Independent Manner ◽

Different Genetic Background

AbstractAntigen-inexperienced memory-like T (AIMT) cells are functionally unique T cells representing one of the two largest subsets of murine CD8+ T cells. However, differences between laboratory inbred strains, insufficient data from germ-free mice, a complete lack of data from feral mice, and unclear relationship between AIMT cells formation during aging represent major barriers for better understanding of their biology. We performed a thorough characterization of AIMT cells from mice of different genetic background, age, and hygienic status by flow cytometry and multi-omics approaches including analyses of gene expression, TCR repertoire, and microbial colonization. Our data showed that AIMT cells are steadily present in mice independently of their genetic background and hygienic status. Despite differences in their gene expression profiles, young and aged AIMT cells originate from identical clones. We identified that CD122 discriminates two major subsets of AIMT cells in a strain-independent manner. Whereas thymic CD122LOW AIMT cells (innate memory) prevail only in young animals with high thymic IL-4 production, peripheral CD122HIGH AIMT cells (virtual memory) dominate in aged mice. Co-housing with feral mice changed the bacterial colonization of laboratory strains, but had only minimal effects on the CD8+ T-cell compartment including AIMT cells.

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Isolation of telomere DNA from Neurospora crassa

Molecular and Cellular Biology ◽

10.1128/mcb.7.9.3168-3177.1987 ◽

1987 ◽

Vol 7 (9) ◽

pp. 3168-3177

Author(s):

M G Schechtman

Keyword(s):

Linkage Group ◽

Neurospora Crassa ◽

Genetic Background ◽

Type Strain ◽

Wild Type Strain ◽

The Other ◽

Wild Type ◽

Oak Ridge ◽

Entire Chromosome ◽

Different Genetic Background

The most distal known gene on Neurospora crassa linkage group VR, his-6, was cloned. A genomic walk resulted in isolation of the telomere at VR. It was obtained from a library in which the endmost nucleotides of the chromosome had not been removed by nuclease treatment before being cloned, and mapping indicates that the entire chromosome end has probably been cloned. Sequences homologous to the terminal 2.5 kilobases of DNA from VR from these Oak Ridge N. crassa strains are found at other sites in the genome. To characterize these sites, I crossed an Oak Ridge-derived his-6 strain with a wild-type strain of different genetic background (Mauriceville) and characterized the hybridization patterns seen in the progeny. It appears that the sequences homologous to the VR terminus are found at genetically different sites in the two parental strains, and no hybridization to the VR telomere from Mauriceville was detected. The other genomic copies identified in the Oak Ridge parent were not telomeres. I suggest that any repeating sequence blocks found immediately adjacent to the VR terminus in Oak Ridge strains must be small and that the repeating element identified in that background may be an N. crassa transposable element integrated near the the chromosome end at VR.

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