scholarly journals Development of 3 Multi-PCR Based Microsatellite Instability Analysis (MSA) for Bladder Cancer Detection

2021 ◽  
Author(s):  
Thomas Reynolds ◽  
Maxie Gordon ◽  
David Moon ◽  
Chulso Moon
Keyword(s):  
Bladder Cancer ◽  
Multiplex Pcr ◽  
Cancer Detection ◽  
Cost Effective ◽  
Microsatellite Analysis ◽  
Comprehensive Analysis ◽  
Johns Hopkins University ◽  
Normal Individuals ◽  
Genetic Assay ◽  
Genetic Analyzer

Abstract Several studies have shown that microsatellite changes can be profiled in urine for the detection of bladder cancer. Use of microsatellite analysis (MSA) for bladder cancer detection requires a comprehensive analysis of up to 15 to 20 markers, which were based on amplification and interpretations of many individual MSA markers and can be technically challenging. Here, in a way to develop fast, more efficient, standardized and less costly MSA for detection of bladder cancer, we have developed 3 multiplex PCR based MSA assay, all of which were analyzed by genetic analyzer. First, we have selected 16 MSA markers based on 9 selected publications. Based on samples from Johns Hopkins University (JHU Sample, first set sample), we have attempted to develop MSA assay based on doublet, 2 tube based multiplex PCR combined with singlet, 1 tube based single plex PCR. While this assay was initially successful, we have encountered a reproducibility issue and we then developed MSA based on triplet, 3 tube based multiplex PCR (Triplet MSA assay). From second set samples (6 cancer patients’ and 14 normal individuals’ sample), our Triplet Assay with 15 MSA markers correctly predicted all of 6/6 cancer samples to be cancerous sample and 14/14 samples from normal individuals as normal samples. This result suggests that our Triplet MSA Assay combined with genetic analyzer is a potentially time and cost-effective genetic assay for bladder cancer detection and can be used potentially as a dependable assay in patient care.

scholarly journals A tissue biopsy-based epigenetic multiplex PCR assay for prostate cancer detection

BMC Urology ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Leander Van Neste ◽  
Joseph Bigley ◽  
Adam Toll ◽  
Gaëtan Otto ◽  
James Clark ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Multiplex Pcr ◽  
Cancer Detection ◽  
Pcr Assay ◽  
Prostate Cancer Detection ◽  
Tissue Biopsy ◽  
Multiplex Pcr Assay

scholarly journals Simultaneous multi-analyte urinary protein assay for bladder cancer detection

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Charles J Rosser ◽  
Yunfeng Dai ◽  
Makito Miyake ◽  
Ge Zhang ◽  
Steve Goodison
Keyword(s):  
Bladder Cancer ◽  
Cancer Detection ◽  
Urinary Protein ◽  
Protein Assay

scholarly journals Development of 3-Hydroxyanthranilic acid-based Integrated Non-invasive Biosensor for Bladder Cancer Detection

2015 ◽  
Vol 26 (2) ◽  
pp. S47-S48
Author(s):  
Yuh-Shyan Tsai ◽  
Yeong-Chin Jou ◽  
Yen-Ping Tsai ◽  
Bin-Da Liu ◽  
Hung-In Lin ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Detection ◽  
Non Invasive ◽  
Hydroxyanthranilic Acid

scholarly journals Electronic Nose for Bladder Cancer Detection

2021 ◽  
Vol 5 (1) ◽  
pp. 22
Author(s):  
Heena Tyagi ◽  
Emma Daulton ◽  
Ayman S. Bannaga ◽  
Ramesh P. Arasaradnam ◽  
James A. Covington
Keyword(s):  
Bladder Cancer ◽  
Logistic Regression ◽  
Random Forest ◽  
Sensitivity And Specificity ◽  
Cancer Detection ◽  
Electronic Nose ◽  
Random Forest Classifier ◽  
Urine Samples ◽  
Sparse Logistic Regression ◽  
High Separation

This study outlines the use of an electronic nose as a method for the detection of VOCs as biomarkers of bladder cancer. Here, an AlphaMOS FOX 4000 electronic nose was used for the analysis of urine samples from 15 bladder cancer and 41 non-cancerous patients. The FOX 4000 consists of 18 MOS sensors that were used to differentiate the two groups. The results obtained were analysed using s MultiSens Analyzer and RStudio. The results showed a high separation with sensitivity and specificity of 0.93 and 0.88, respectively, using a Sparse Logistic Regression and 0.93 and 0.76 using a Random Forest classifier. We conclude that the electronic nose shows potential for discriminating bladder cancer from non-cancer subjects using urine samples.

scholarly journals Gender Differences in Pathologic Outcomes At Primary Bladder Cancer Detection

2015 ◽  
Author(s):  
Rahmi Gökhan Ekin ◽  
Zübeyde Yıldırım Ekin ◽  
Gökhan Koç ◽  
Ayşe Gülden Diniz Ünlü ◽  
Yusuf Özlem İlbey ◽  
...  
Keyword(s):  
Gender Differences ◽  
Bladder Cancer ◽  
Cancer Detection ◽  
Primary Bladder ◽  
Primary Bladder Cancer

scholarly journals Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay

2000 ◽  
Vol 38 (11) ◽  
pp. 4114-4120 ◽  
Author(s):  
WanHong Xu ◽  
Mike C. McDonough ◽  
Dean D. Erdman
Keyword(s):  
Multiplex Pcr ◽  
Human Adenovirus ◽  
Cost Effective ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Amplicon Size ◽  
Species Specific ◽  
Fiber Gene ◽  
Amplification Reaction

A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.

scholarly journals AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs

Sensors ◽  
2020 ◽  
Vol 20 (15) ◽  
pp. 4075
Author(s):  
Ji-Eun Lee ◽  
Woo-Jin Song ◽  
Hunjoo Lee ◽  
Byung-Gak Kim ◽  
Taeho Kim ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Cancer Detection ◽  
Malignant Tumors ◽  
Cost Effective ◽  
Benign Tumors ◽  
Dependent Protein Kinase ◽  
Diagnostic Significance ◽  
Dependent Protein ◽  
Kinase A

The early detection of tumors improves chances of decreased morbidity and prolonged survival. Serum biomarkers are convenient to use and have several advantages over other approaches, such as accuracy and straightforward protocols. Reliable biomarkers from easily accessible sources are warranted for the development of cost-effective assays for routine screening, particularly in veterinary medicine. Extracellular c-AMP-dependent protein kinase A (ECPKA) is a cytosolic leakage enzyme. The diagnostic accuracy of detecting autoantibodies against ECPKA was found to be higher than that of ECPKA activity from enzymatic assays, which use a complicated method. Here, we investigated the diagnostic significance of measuring serum ECPKA autoantibody levels using an in-house kit (AniScan cancer detection kit; Biattic, Anyang, Korea). We used sera from 550 dogs, including healthy dogs and those with malignant and benign tumors. Serum ECPKA and immunoglobulin G were determined using the AniScan cancer detection kit. ECPKA autoantibody levels were significantly higher (p < 0.01) in malignant tumors than in benign tumors, non-tumor diseases, and healthy controls. On the basis of sensitivity and specificity values, AniScan ECPKA is a rapid and easy-to-use assay that can be applied to screen malignant tumors from benign tumors or other diseases in dogs.

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