scholarly journals CYTOLOGICAL EXAMINATION OF EXPERIMENTAL PURULENT WOUNDS IN THE TREATMENT OF SILVER NANOPARTICLES IN ULTRASOUND CAVITATION

2019 ◽  
Vol 7 (4) ◽  
pp. 386-395
Author(s):  
P. F. Myronov ◽  
V. I. Bugaiov ◽  
O. O. Tymakova ◽  
M. V. Pogorielov ◽  
A. S. Opanasyuk

The treatment of purulent wounds is an important problem of modern surgery. Antibiotic resistance of bacteria dramatically reduces the effectiveness of traditional methods of treatment. Previous studies have indicated that silver nanoparticles have good antibacterial activity and do not cause bacterial resistance. Low-frequency ultrasound improves the bactericidal properties of nanoparticles, as well as plays an important role in cleaning wounds from purulent-necrotic tissue and delivering nanoparticles to the site of infection. The combination of the properties of silver nanoparticles and low-frequency ultrasound requires careful investigation in the treatment of purulent wounds. The purpose of the study was to substantiate the effectiveness of the treatment of purulent wounds with silver nanoparticles and low-frequency ultrasound by cytological examination. Materials and Methods. This study was carried out on 60 laboratory rats, which were equally divided into 3 groups. In the first group, treatment was carried out by low-frequency ultrasound; in the second group, a solution of silver nanoparticles was used together with low-frequency ultrasound; in the third, control group, a 0.05% Chlorhexidine solution was used. Silver nanoparticles with a size of 10-60 nm were synthesized by the polyol method. Results. The study showed that there were no statistically significant differences between the ultrasound, silver nanoparticles/ultrasound and Chlorhexidine groups on the first day. On the third day, a statistically significant increase in phagocytic neutrophilic leukocytes and fibroblasts was observed in the silver nanoparticles/ultrasound group compared with the first day. In comparison with the Chlorhexidine group, the number of monocytes (2.2 and 4.2 times) and macrophages (1.4 and 1.9 times) increased in the ultrasound and silver nanoparticles/ultrasound groups respectively, and the necrotic type of cytograms was not determined. On the seventh day, granulation tissue began to appear in the silver nanoparticles/ultrasound group, the percentage of leukocyte destruction decreased (by 4.5 times), microorganisms were almost not detected, regenerative types of cytograms appeared for the first time. The number of neutrophils in the control group exceeded the analogous parameter of the ultrasound group by 2.4 times and the silver nanoparticles/ultrasound group by 3.8 times. The number of fibroblasts became significantly larger in the ultrasound (2.2 times) and the silver nanoparticles/ultrasound (2.3 times) groups, compared with the group where Chlorhexidine was used. On the tenth day, the number of fibroblasts and cells of the monocytic-macrophage series increased in the control group, which indicates later regenerative processes. On the tenth day, there was a complete epithelization of wounds in the silver nanoparticles/ultrasound group, while healing occurred on day 12 in the ultrasound group, and on day 21 in the control group. Conclusions. The combined use of silver nanoparticles and low-frequency ultrasound significantly improves the cytological parameters of wound healing of purulent wounds and has clear advantages over the ultrasound monotherapy and the use of Chlorhexidine. The presented method reduces the treatment time and can be prospectively introduced into surgical practice.

2012 ◽  
Vol 93 (2) ◽  
pp. 287-289 ◽  
Author(s):  
I S Malkov ◽  
E K Salakhov

Aim. To develop a method of programmed laparoscopic sanitation in patients with generalized forms of peritonitis with the use of low-frequency ultrasound energy. Methods. Conducted was an analysis of results of treatment of 211 patients with generalized peritonitis admitted to the surgical department of the City Clinical Hospital №7 from 2000 to 2010. The main group comprised 37 patients in whom laparoscopic sanitation with the use of low-frequency ultrasound was performed. The control group included 174 patients who underwent conventional relaparotomy due to progression of the pathological process in the abdominal cavity. Results. The proposed method of laparoscopic sanitation helped to reduce mortality by 12%, and the development of postoperative complications by 8% compared with control group in patients of the II stage of severity of peritonitis according to the Mannheim peritoneal index. Conclusion. The developed method of programmed videolaparoscopic sanitations of the abdominal cavity with the use of low-frequency ultrasound in patients with generalized peritonitis has a pronounced bactericidal and bacteriostatic effect.


2012 ◽  
Vol 1 (1) ◽  
pp. 9-12
Author(s):  
Syeda Amool Sakeena Rizvi ◽  
Syed Abid Mehdi Kazmi ◽  
Sumaira Imran Farooqi ◽  
Jharna Devi

OBJECTIVE To compare the effectiveness of low and high frequency ultrasound (US) technique for treatment of osteoarthritis (OA) of knee joint. It is a pre-post design was used with multiple treatment therapies (A and B). The study was conducted on the 310 patients. The patients were diagnosed knee OA by the orthopedic doctor and recommended to the Physiotherapy department of North and Clifton Campus of Ziauddin Hospital. In this study 310 patients were divided into two groups. Group A of 155 patients received low frequency US treatment of 0.01MHz frequency for 10 min and at the same time Group B of 155 patients received high frequency US treatment of 1MHz for about 3 min. Both the groups had 6 sessions in 3 weeks. There was a mean reduction in the pain and disability with difference of low frequency US and high frequency US pre score and post score treatments of both the groups is 1.2± 0.3 and 4.1± 1.0, there was statistically significant difference between the effects of low frequency US and high frequency US in pain management of knee O.A (P < 0.05). There was difference between the effectiveness of low frequency US


2020 ◽  
Vol 10 (4) ◽  
pp. 621-631
Author(s):  
Petro Myronov ◽  
Volodymyr Bugaiov ◽  
Viktoriia Holubnycha ◽  
Vladyslav Sikora ◽  
Volodymyr Deineka ◽  
...  

2015 ◽  
Vol 69 (3-4) ◽  
pp. 14-18
Author(s):  
Z. V. Gasimova

Background: Goal of the research was to study occurrence frequency of impacted teeth, to develop rational methods of diagnostics and treatment of denotalveolar and facial anomalies caused by impacted teeth. Patients and methods: From 1989 for 2013 period of time 7172 patients with dentoalveolar anomalies, referred to our clinic for the orthodontic help were surveyed. Results: At 899 (12,53±0,39%) patients it is revealed 1405 impacted teeth, from them on the maxilla — 623, on mandible — 752 teeth. The most widespread impacted teeth on the maxilla were canines, on the mandible — the second premolars and the third molars. Treatment of impacted teeth by stimulation of their eruption by low-frequency ultrasound with a frequency of fluctuations of 26,5 kHz, intensity of 1 W/cm, with an amplitude of 40–60 microns and wave of 0,012 m long, daily or within 1 days, with period of 3–10 seconds with the course of treatment averaging 1–5 procedures, until a tooth eruption is carried out. Conclusion: The way of stimulation developed by us for late erupted impacted permanent teeth on the basis of application physiologic for an organism low-frequency ultrasound promotes increase of efficiency and reduction of treatment terms of dentoalveolar and facial anomalies. The received positive results allow to recommend this method for adoption in orthodontic practice.


2020 ◽  
pp. 13-23
Author(s):  
Natalya Kornova ◽  
A. Krylov

The article presents results of a study on the effectiveness of using lowfrequency ultrasound and photochromotherapy in the complex treatment of patients with chronic bacterial and viral rhinosinusitis. The study involved 69 people aged 18 to 55 years: 39 (56.83 %) women and 30 (45.11 %) men with chronic rhinosinusitis during the period of clinical exacerbation and concomitant chronic tonsillitis without exacerbation. All patients included in the study underwent X-ray examination or computed tomography and magnetic resonance imaging of the paranasal sinuses. 100 % of the patients were examined for the presence of antibodies to Human gammaherpesvirus 4 (ELISA), had study of blood serum for IgM and IgG and test to determine the avidity of Ig G antibodies to γ-herpesviruses. The obtained data indicate the effectiveness of including low-frequency ultrasound and photochromotherapy in the complex treatment of patients with chronic rhinosinusitis and concomitant chronic tonsillitis of bacterial and viral nature without exacerbation.


Author(s):  
Mohd Razealy Anuar ◽  
Mohamed Hussein Abdurahman ◽  
Nor Irwin Basir ◽  
Ahmad Zuhairi Abdullah

2003 ◽  
Vol 29 (5) ◽  
pp. S120-S121
Author(s):  
S. Bashardoust Tajali ◽  
S. Kazemi ◽  
A. Azari ◽  
A. Shahverdi ◽  
M. Jabal Ameli

Sign in / Sign up

Export Citation Format

Share Document