scholarly journals SHORT TERM STORAGE OF PLUG-GROWN BEDDING PLANT SEEDLINGS.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1094c-1094
Author(s):  
M. P. Kaczperski ◽  
A. M. Armitage
Keyword(s):  
Petunia Hybrida ◽  
Growth Habit ◽  
Storage Period ◽  
Storage Conditions ◽  
Short Term ◽  
Salvia Splendens ◽  
Time Period ◽  
Bedding Plant ◽  
Plant Seedlings ◽  
Term Storage

The effects of differing storage conditions prior to transplanting were examined for Salvia splendens `Red Hot Sally', Impatiens wallerana `Super Elfin White', Viola × wittrockiana `Universal Beaconsfield' and Petunia × hybrida `Supercascade Lilac'. Plug-grown seedlings were stored for 0, 1, 2 or 3 weeks at 5C or 10C and irradiance levels from incandescent bulbs at 0, 2 or 12 μmol s-1 m-2. A second group of plants were stored at 18C and irradiance from fluorescent bulbs at 105 μmol s-1 m-2 for the same time period. Temperature was more important than irradiance in maintaining plant quality over the storage period. Impatiens and salvia could be stored successfully for a minimum of 2 weeks at 5 or 10C with no appreciable loss of quality, petunia and pansy up to 3 weeks. Seedlings of all species showed diminished quality when stored longer than 1 week at 18C. After storage, petunias stored at 18C flowered sooner than those stored at 5 or 10C. However, these plants were single stemmed, with long internodes and few flowers while those plants stored at 5 or 10C developed multiple branching and a short, compact growth habit at flowering.

scholarly journals Short-term Storage of Plug-grown Bedding Plant Seedlings

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 798-800 ◽  
Author(s):  
M.P. Kaczperski ◽  
A.M. Armitage
Keyword(s):  
Petunia Hybrida ◽  
Storage Period ◽  
Storage Conditions ◽  
Production Environment ◽  
Short Term ◽  
Salvia Splendens ◽  
Bedding Plant ◽  
Short Term Storage ◽  
Plant Seedlings ◽  
Term Storage

The effects of storage conditions before transplanting were examined for Petunia × hybrida Vilm. `Supercascade Lilac', viola × wittrockiana Gams `Universal Beaconsfield', and Salvia splendens F. Sellow ex Roem. & Schult `Red Hot Sally'. Plug grown seedlings were stored for 0, 7, 14, or 21 days at 5 or 10C and with continuous irradiance levels from incandescent bulbs at 0, 2, or 12 μmol·m-2·s-1. A second group was stored at 18C with irradiance from fluorescent bulbs at 105 μmol·m-2·s-1 and a 16-hour photoperiod for the same durations. Temperature was more important than irradiance in maintaining a commercially acceptable plant during the storage period. Petunia and pansy could be stored successfully for 21 days at 5 or 10C with no appreciable loss of quality; salvia could be stored for a minimum of 14 days. Seedlings of all species elongated excessively when stored >7 days at 18C and 105 μmol·m-2·s-1 irradiance. After 14 days of storage, petunias stored at 18C flowered sooner than those stored at 5 or 10C but time in a production environment (days to flower - days in storage) was similar for petunias stored at 5 or 18C.

scholarly journals Impact of Thermotherapy and Short-Term Storage on Quercus robur L. Acorn Mycobiota and Germination

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 528
Author(s):  
Jelena Kranjec Orlović ◽  
Damir Drvodelić ◽  
Marko Vukelić ◽  
Matea Rukavina ◽  
Danko Diminić ◽  
...  
Keyword(s):  
Natural Regeneration ◽  
Fungal Diversity ◽  
Quercus Robur ◽  
Negative Impact ◽  
Storage Conditions ◽  
Short Term ◽  
Isolation Frequency ◽  
Short Term Storage ◽  
Penicillium Spp ◽  
Term Storage

When natural regeneration of Quercus robur stands is hampered by an insufficient acorn yield, human assisted sowing of acorns collected in non-affected stands and stored for some period of time is performed. To inhibit the development of fungi and acorn deterioration during storage, thermotherapy is usually applied by submerging acorns for 2.5 h in water heated to 41 °C. This research aimed to test the effect of four thermotherapy treatments of different durations and/or applied temperatures as well as short-term storage at −1 °C or 3 °C on acorn internal mycobiota and germination. Fungal presence in cotyledons was analyzed in 450 acorns by isolation of mycelia on artificial media, followed by a DNA-based identification. Germination of 2000 acorns was monitored in an open field trial. Thermotherapy significantly decreased fungal diversity, while storage at 3 °C increased the isolation frequency of several fungi, mainly Penicillium spp. The most frequently isolated fungi did not show a negative impact on acorn germination after short-term storage. The study confirmed the efficiency of thermotherapy in the eradication of a part of acorn internal mycobiota, but also its effect on the proliferation of fast-colonizing fungi during storage. However, the latter showed to be more stimulated by storage conditions, specifically by storage at 3 °C.

scholarly journals Management of Apple Maturity and Postharvest Storage Conditions to Increase Polyphenols in Cider

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart
Keyword(s):  
The United States ◽  
Storage Conditions ◽  
Short Term ◽  
Postharvest Storage ◽  
Total Polyphenols ◽  
Fruit Storage ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

349 IMPROVING THE FUNCTIONALITY OF STORED FLOW CYTOMETRICALLY SEX-SORTED BOAR SPERMATOZOA: SEDIMENTATION VS. CENTRIFUGATION

2006 ◽  
Vol 18 (2) ◽  
pp. 282 ◽  
Author(s):  
I. Parrilla ◽  
J. M. Vazquez ◽  
M. A. Gil ◽  
E. M. Garcia ◽  
I. Caballero ◽  
...  
Keyword(s):  
Detrimental Effect ◽  
Storage Period ◽  
Recovery Efficiency ◽  
Cell Recovery ◽  
Routine Procedure ◽  
Short Term ◽  
Short Term Storage ◽  
Boar Spermatozoa ◽  
Casa System ◽  
Term Storage

Centrifugation has been the routine procedure used to concentrate the highly diluted spermatozoa obtained after sorting. However, this harmful procedure has a detrimental effect on the lifespan of the spermatozoa, particularly when a short-term storage period is required to perform the inseminations in the farms. The aim of the study was to evaluate the procedure of concentration by sedimentation of the flow cytometrically sex-sorted boar spermatozoa after a 24-h storage period, in terms of sperm functionality and recovery efficiency. Spermatozoa were sex-sorted by flow cytometry (Johnson and Welch 1999 Theriogenology 52, 1323-1342) and collected in 50-mL tubes containing 5 mL of Beltsville thawing solution (BTS) with 10% seminal plasma (SP). The collected samples were split into two aliquots. The first aliquot was concentrated by centrifugation (800g/5 min) just after sorting and stored at 17�C for 24 h at a concentration of 6 � 106 sperm/mL. The second aliquot was directly stored (0.5 � 106 sperm/mL) at 17�C for 24 h. In this case, after storage the supernatant was discarded and the pellet obtained by sedimentation was adjusted to 6 � 106 sperm/mL. For assessment of the sperm functionality after the storage, motility and viability of the spermatozoa were analyzed using a CASA system (Sperm Class Analyzer; Microptic, Barcelona, Spain) and a EPICS XL flow cytometer (Coulter Corporation, Inc., Miami, FL, USA) after the sperm were stained with propidium iodide and SYBR-14, respectively. For assessment of the recovery efficiency, the total number of spermatozoa in each sample was counted. The results are expressed as percentages of five replicates. Statistical analysis was performed using a �2 test. Percentages of motile and viable spermatozoa just after sorting were 80% and 83.3%, respectively. After storage at 17�C for 24 h, the percentage of viable spermatozoa was significantly higher (P < 0.05) in settled spermatozoa (82.4%) compared to centrifuged spermatozoa (63.3%). Similarly, the percentage of motile spermatozoa was significantly higher (P < 0.05) in settled spermatozoa (78.3%) compared to centrifuged spermatozoa (58.3%), as was the percentage of progressive motility (63.6% vs. 35.5%, respectively; P < 0.05). Interestingly, the percentage of cell recovery was significantly higher (P < 0.05) after sedimentation (80% of the sorted population) than after centrifugation (60.3% of the sorted population). The results show that sedimentation is more beneficial than centrifugation when short-term storage of sex-sorted boar sperm is required. This work was supported by CDTI and Fundaci�n Seneca.

scholarly journals Evaluation of sweet cherry fruit quality after short-term storage in relation to the rootstock

2019 ◽  
Vol 60 (6) ◽  
pp. 925-934 ◽  
Author(s):  
Ewa Dziedzic ◽  
Jan Błaszczyk
Keyword(s):  
Fruit Quality ◽  
Sweet Cherry ◽  
Fungal Infections ◽  
Weather Conditions ◽  
Storage Conditions ◽  
Soluble Solids ◽  
Short Term ◽  
Fruit Storage ◽  
Short Term Storage ◽  
Term Storage

Abstract Fruits of the sweet cherry cultivar ‘Regina’ collected from trees growing on seven rootstocks were stored in a cold room at 2 °C with a normal (NA) and controlled atmosphere (15% and 20% CO2 and 5% O2—CA1 and CA2) for 2 weeks. The rootstocks on which the trees grew and the storage conditions significantly affected all fruit parameters tested during both years of the experiment. Fruit from Damil rootstock exhibited higher mean firmness than fruit from Colt rootstock. The effect of rootstocks on the value of soluble solids content (SSC) varied, wherein the fruits from Tabel Edabriz and Damil were characterized by high SSC mean content. The organic acids content (TA) was significantly lower after storage than during harvest time. Fruits from Tabel Edabriz trees were characterized by faster ripening, as was evident by the higher SSC to TA ratio. The amount of mass lost depended significantly and only on the storage conditions—sweet cherries from both CA combinations had the lowest mass losses. The percentage of fruits showing disease symptoms was largely dependent on the weather conditions in the orchard the year before the fruit harvest, as well as atmosphere composition and RH during fruit storage. Cold storage conditions with a high (20%) CO2 content are recommended for the short-term storage of sweet cherry fruits because they preserve fruit quality parameters: a low decrease in firmness, maintenance of a high SSC/TA ratio, a low percent of fungal infections, and good preservation of green color in the peduncle.

scholarly journals Physiological, Biochemical, and Molecular Changes in Pelargonium Cuttings Subjected to Short-term Storage Conditions

1996 ◽  
Vol 121 (6) ◽  
pp. 1063-1068 ◽  
Author(s):  
Richard N. Arteca ◽  
Jeannette M. Arteca ◽  
Tzann-Wei Wang ◽  
Carl D. Schlagnhaufer
Keyword(s):  
Gene Expression ◽  
Acc Oxidase ◽  
Storage Conditions ◽  
Significant Decline ◽  
Short Term ◽  
Molecular Changes ◽  
Visual Rating ◽  
Shoot Weight ◽  
Term Storage

The purpose of this study was to evaluate physiological, biochemical, and molecular changes that occur in unrooted Pelargonium ×hortorum cuttings during storage. Pelargonium cuttings of `Sincerity' (good shipper), `Wendy Ann' (moderate shipper) and `Snowmass' (poor shipper) were stored at 25 °C and evaluated over a 5-day period. Following removal from storage, cuttings of all cultivars exhibited steady and significant decline in photosynthesis, respiration, carbohydrate, starch, and protein over time. However, no significant differences were observed among cultivars for all of these parameters. Ethylene levels produced by `Sincerity' and `Wendy Ann' began to increase 3 days following storage; whereas, `Snowmass' showed an increase after 1 day, reaching a peak at 3 days, and then declined. When unrooted cuttings of `Snowmass' were stored for 5 days at temperatures ranging from 4 to 25 °C, it was observed that those stored at 4 °C had a significantly higher visual rating, chlorophyll content, and root and shoot weight than at higher temperatures tested. As temperature increased from 10 to 25 °C, quality of cuttings declined. Changes in gene expression of two ACC synthases and an ACC oxidase were evaluated in `Snowmass' cuttings stored at 4 and 25 °C. Correlations between ethylene and ACC levels with gene expression were observed. Chemical name used: 1-aminocyclopropane-1-carboxylic acid (ACC).

scholarly journals Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11933
Author(s):  
Adrian Simpson ◽  
Aaron Topol ◽  
Bradley J. White ◽  
Marlene K. Wolfe ◽  
Krista R. Wigginton ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Storage Conditions ◽  
Mottle Virus ◽  
Surveillance Program ◽  
Short Term ◽  
Pepper Mild Mottle Virus ◽  
Strong Interest ◽  
Rna Quantification ◽  
Term Storage

SARS-CoV-2 RNA in wastewater settled solids is associated with COVID-19 incidence in sewersheds and therefore, there is a strong interest in using these measurements to augment traditional disease surveillance methods. A wastewater surveillance program should provide rapid turn around for sample measurements (ideally within 24 hours), but storage of samples is necessary for a variety of reasons including biobanking. Here we investigate how storage of wastewater solids at 4 °C, −20 °C, and −80 °C affects measured concentrations of SARS-CoV-2 RNA. We find that short term (7 or 8 d) storage of raw solids at 4 °C has little effect on measured concentrations of SARS-CoV-2 RNA, whereas longer term storage at 4 °C (35–122 d) or freezing reduces measurements by 60%, on average. We show that normalizing SARS-CoV-2 RNA concentrations by concentrations of pepper mild mottle virus (PMMoV) RNA, an endogenous wastewater virus, can correct for changes during storage as storage can have a similar effect on PMMoV RNA as on SARS-CoV-2 RNA. The reductions in SARS-CoV-2 RNA in solids during freeze thaws is less than those reported for the same target in liquid influent by several authors.

scholarly journals Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 806
Author(s):  
Aitor Fernandez-Novo ◽  
Sergio Santos-Lopez ◽  
Clara Barrajon-Masa ◽  
Patricia Mozas ◽  
Eduardo de Mercado ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Room Temperature ◽  
Storage Time ◽  
Storage Conditions ◽  
Short Term ◽  
Bull Semen ◽  
The Impact ◽  
Term Storage ◽  
Diagnostic Centre ◽  
Holding Temperature

CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 °C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 °C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.

scholarly journals Initial Short-term Storage at 33 °F Reduces Physiological Disorder Development in ‘Honeycrisp’ Apples

2018 ◽  
Vol 28 (4) ◽  
pp. 481-484 ◽  
Author(s):  
Yosef Al Shoffe ◽  
Christopher B. Watkins
Keyword(s):  
Low Temperature ◽  
Specific Time ◽  
Short Term ◽  
Physiological Disorder ◽  
Time Period ◽  
Bitter Pit ◽  
Short Term Storage ◽  
Honeycrisp Apples ◽  
Soft Scald ◽  
Term Storage

Initial short-term storage is a treatment where fruit are cooled to 33 °F for a specific time period and then moved to 38 °F until the end of storage. Its effects on the development of physiological disorders in ‘Honeycrisp’ apples (Malus domestica) were investigated for two seasons. During the first season, fruit were harvested from two orchards and stored at 33 and 38 °F, with and without 1 week of conditioning at 50 °F, or stored for 4 weeks at 33 °F followed by 4 weeks at 38 °F. All fruit were stored for a total of 8 weeks. In the second season, fruit were harvested from one orchard and stored at 38 °F either with or without 1 week of conditioning at 50 °F, or stored for 1 week at 33 °F and moved to 38 °F for 15 weeks followed by 7 d at 68 °F. Short-term storage (1 to 4 weeks) at 33 °F decreased bitter pit for all orchards in the two seasons, except in comparison with the continuous 33 °F storage in the first season; soft scald was also reduced in the first season compared with continuous storage at 33 °F, with higher incidence of soft scald in orchard one compared with orchard two. Initial short-term storage at 33 °F resulted in lower soggy breakdown incidence compared with storage at 33 °F with 1 week of conditioning at 50 °F for fruit from orchard two in the first season, the only year when low-temperature injuries were observed. In conclusion, initial short-term storage at 33 °F followed by storage at 38 °F maintained the highest percentage of healthy fruit in the two seasons.

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