Germination and Storage of Vinca Seed Is Influenced by Light, Temperature, and Relative Humidity

Light, temperature, relative humidity (RH), and GA3 affect vinca [Catharanthus roseus (L.) G. Don] seed storage and/or germination. GA3 failed to increase the germination percentage in darkness but significantly increased the percentage in continuous light. Similarly, GA3 treatment reduced both the number of days required to achieve 50% of the final germination percentage (T50) and the span between 10% and 90% germination (T90 — T10) for seeds in light, but not in darkness. Germination percentages were maximal and about equal at 25, 30, or 35C in darkness; germination was lowest below 25C. Germination T50 and T90 — T10 required the fewest days between 25 and 35C. Reducing seed moisture from 9.9% to 3.9% increased the T50 from 2.4 to 3.0 days but failed to change germination percentages. Germination percentage declined linearly as seed storage temperatures were reduced from 5 to — 20C, whereas days to T50 increased. Seed storage for 12 months without reduction in germination percentage was possible at 5C and 11%, 33%, or 52% RH, but storage at 75% or 95% RH for periods exceeding 1 month reduced germination. Seeds stored at 33% or 52% RH required fewer days to T50 than did seeds stored at 11%, 75%, or 95% RH. Chemical name used: gibberellic acid (GA3).

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Growth Regulators and Storage Temperature Govern Germination of Coreopsis Seed

HortScience ◽

10.21273/hortsci.27.11.1190 ◽

1992 ◽

Vol 27 (11) ◽

pp. 1190-1193 ◽

Cited By ~ 2

Author(s):

William J. Carpenter ◽

Eric R. Ostmark

Keyword(s):

Relative Humidity ◽

Gibberellic Acid ◽

Growth Regulators ◽

Storage Temperature ◽

Seed Storage ◽

Temperature Regimes ◽

Alternating Temperature ◽

And Storage ◽

Storage Temperatures ◽

Growth Regulator Treatment

The storage and germination environments were evaluated to determine the cause of low total germination percentages and highly irregular germination of Coreopsis lanceolata L. seed. Highest total germination and most rapid and uniform germination of seed occurred at constant 15C, other constant temperatures and all alternating temperature regimes caused lower total germination or delayed it. Seeds tolerated -20C during storage, but total germination was reduced below -5C. Recently harvested seeds had 44% total germination, but 54% to 81% germination was achieved after 6 hours of soaking seeds in 1000 ppm GA3, 1000 ppm ethephon, or 25 ppm kinetin alone or in combination. Growth regulators reduced the number of days to 50% of final germination (T50), and the span in days between 10% and 90% of germination (T90 - T10). Storing fresh seeds without chemical treatment for > 6 months at 5C and 10% to 20% relative humidity (RH), or 15C at 20% to 35% RH, increased total germination to 75% and 80%, respectively. Ten days were required to achieve T50 after 5 to 6 months of storage at 5C and 10% to 20% RH or 15C and 10% to 40% RH, with longer periods to T50 at other storage durations and RH levels. The germination spans (T90 - T10) were lengthened the higher the seed storage temperatures between 5 to 25C, with longer spans as seed storage durations and relative humidities increased. Total germination was similar after storing seeds at 5 or 15C and 10% to 30% RH and after soaking recently harvested seeds in GA3 + ethephon, but the days to T50 and T90 - T10 were shorter after growth regulator treatment. Chemical names used: (2-chloroethyl) phosphonic acid (ethephon); gibberellic acid (GA3); 6-furfurylaminopurine (kinetin).

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Temperature and Relative Humidity Govern Germination and Storage of Gladiolus Seed

HortScience ◽

10.21273/hortsci.26.8.1054 ◽

1991 ◽

Vol 26 (8) ◽

pp. 1054-1057 ◽

Cited By ~ 1

Author(s):

W.J. Carpenter ◽

G.J. Wilfret ◽

J.A. Cornell

Keyword(s):

Relative Humidity ◽

Seed Treatment ◽

No Reduction ◽

Germination Rate ◽

Seed Viability ◽

Seed Storage ◽

Moisture Contents ◽

Seed Moisture ◽

Germination Temperature ◽

And Storage

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.

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Role of Relative Humidity in Processing and Storage of Seeds and Assessment of Variability in Storage Behaviour inBrassicaspp. andEruca sativa

The Scientific World JOURNAL ◽

10.1155/2013/504141 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

A. Suma ◽

Kalyani Sreenivasan ◽

A. K. Singh ◽

J. Radhamani

Keyword(s):

Relative Humidity ◽

Seed Viability ◽

Seed Storage ◽

Eruca Sativa ◽

Seed Moisture Content ◽

Seedling Vigour ◽

Seed Moisture ◽

Processing And Storage ◽

And Storage

The role of relative humidity (RH) while processing and storing seeds ofBrassicaspp. andEruca sativawas investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species ofBrassicawas also evaluated. The samples were stored at40±2°Cin sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour,B. rapaandB. junceawere better performers thanB. napusandEruca sativa.

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Evaluation of Temperature and Moisture Content during Storage on the Germination of Flowering Annual Seed

HortScience ◽

10.21273/hortsci.30.5.1003 ◽

1995 ◽

Vol 30 (5) ◽

pp. 1003-1006 ◽

Cited By ~ 4

Author(s):

William J. Carpenter ◽

Eric R. Ostmark ◽

John A. Cornell

Keyword(s):

Relative Humidity ◽

Moisture Content ◽

Seed Storage ◽

Seed Moisture Content ◽

Moisture Contents ◽

Seed Moisture ◽

Storage Temperatures

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.

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Conservation of Garcinia imberti Bourd. through seeds

Plant Science Today ◽

10.14719/pst.2019.6.2.518 ◽

2019 ◽

Vol 6 (2) ◽

pp. 243-251

Author(s):

M Anto ◽

M Angala ◽

P S Jothish ◽

C Anilkumar

Keyword(s):

Seed Germination ◽

Relative Humidity ◽

Moisture Content ◽

Gibberellic Acid ◽

Biosphere Reserve ◽

Seed Storage ◽

Seed Moisture Content ◽

Evergreen Forests ◽

Seed Moisture ◽

Subsequent Exposure

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.

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Light and Temperature Govern Germination and Storage of Caladium Seed

HortScience ◽

10.21273/hortsci.25.1.71 ◽

1990 ◽

Vol 25 (1) ◽

pp. 71-74 ◽

Cited By ~ 3

Author(s):

William J. Carpenter

Keyword(s):

No Reduction ◽

Seed Storage ◽

Germination Percentage ◽

Incandescent Light ◽

Fruit Abscission ◽

Moisture Contents ◽

Seed Moisture ◽

And Storage ◽

Light Maximum

Caladium hortulanum Birdsey cv. Candidum seed failed to germinate without light; maximum germination required daily, incandescent light of ≤4 hours. Lengthening daily lighting periods progressively reduced the days to 50% relative germination (T50) from 20 to 8, and days between 10% and 90% relative germination (T90 – T10) from 16 to 5. T50 and T90 – T10 were shortest (≈ 8 days) at 25 and 30C, while total or absolute germination percentage (G) was highest at ≈ 90%. G was 94% for seeds harvested immediately, but 75% or 38% for seeds that remained in fruits for 3 or 12 weeks after fruit abscission from the spadix. Total absolute germination was reduced from 95% to 87% when seed moisture contents declined to <14%. Seed storage for 7 days at from 10 to – 80 C-caused no reduction in G. Seeds were stored 6 months at 15C and 22%, 33%, or 52% RH without change in G, but storage at 5 or 25C and 11%, 75%, or 95% RH significantly reduced germination.

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Temperature and Seed Moisture Govern Germination and Storage of Gerbera Seed

HortScience ◽

10.21273/hortsci.30.1.98 ◽

1995 ◽

Vol 30 (1) ◽

pp. 98-101 ◽

Cited By ~ 4

Author(s):

William J. Carpenter ◽

Eric R. Ostmark ◽

John A. Cornell

Keyword(s):

Relative Humidity ◽

Low Temperature ◽

Seed Storage ◽

Gerbera Jamesonii ◽

Mean Temperature ◽

Seed Moisture ◽

And Storage

Temperature, relative humidity (RH), desiccation, and hydration affect gerbera (Gerbera jamesonii H Bolus ex Hook.f.) seed storage and germination. Germination percentages (G) were maximal and about equal at constant 15, 20, or 25C in darkness or light but lower at alternating temperatures having the same mean temperature. The number of days to 50% final germination (T50) and between 10% and 90% germination (T90 – T10) required the fewest days at constant 25 or 30C; longer germination periods resulted with alternating temperatures. Reducing seed moisture from 7.1% to 3.5% had no effect on G, T50, or T90 – T10 values, but at seed moisture levels <3.5%, G was lower and T50 and T90 – T10 longer. Germination percentages were similar after seed storage from 5 to –5C, but G was lower after storage at –10C or lower. Low-temperature seed storage had no effect on T50 or T90 – T10 values. Seeds had highest G and lowest T50 and T90 – T10 values when germinated at 52% seed moisture, with large declines and delays in germination at lower and higher moisture levels. Seed storage for 12 months without reduction in germination was possible at 5C and 11% or 32% RH. Seeds stored at 52% RH lost G at all temperatures, and no seed germinated after storage at 75% RH and 15 or 25C. Seed stored at 5 or 15C and 11% to 32% RH had the fewest days to T50 and T90 – T10.

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Effects of gibberellic acid and storage temperature on the germination of hawthorn seeds

Journal of Forest Science ◽

10.17221/24/2017-jfs ◽

2017 ◽

Vol 63 (No. 9) ◽

pp. 417-424 ◽

Cited By ~ 1

Author(s):

Ahmadloo Fatemeh ◽

Kouchaksaraei Masoud Tabari ◽

Goodarzi Gholam Reza ◽

Salehi Azadeh

Keyword(s):

Electrical Conductivity ◽

Gibberellic Acid ◽

Water Uptake ◽

Temperature Regime ◽

Storage Temperature ◽

Fresh Weight ◽

Freeze Thaw ◽

Refrigerator Temperature ◽

And Storage ◽

Storage Temperatures

This study investigated methods to overcome seed dormancy in Crataegus pseudoheterophylla Pojarkova seeds. Seeds with and without endocarps were treated with gibberellic acid (GA3) at different concentrations and four storage temperatures. Then they were stratified in an alternate temperature regime. The amount of absorbed water in seeds with endocarps was monitored by measuring the fresh weight of seeds for 0, 24, 48, 72, and 96 h of imbibition. The electrical conductivity (EC) and the percentage of water uptake by seeds stored for 12 months at laboratory temperature, in a refrigerator, in a freezer, and in freeze-thaw conditions were measured. The highest germination (59.7%) was recorded in seeds without endocarps treated with 3,000 mg·l–1 GA3 and stored either in a laboratory or a refrigerator (32.7–35.3%). All treatments of seeds without endocarps where GA3 was applied showed statistically higher percentages of germination than the control. Seeds with endocarps stored at refrigerator temperature imbibed water up to 44.3% with increasing imbibition periods, whereas the amount of seeds that absorbed water in freezer and freeze-thaw conditions was almost the same. The tests showed the highest EC during storage in the freezer, with the lowest water uptake and viability in seeds stored during the freeze-thaw process.

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Studies to extend the dormancy of white yam (Dioscorea alata L)

The Journal of Agriculture of the University of Puerto Rico ◽

10.46429/jaupr.v74i3.6653 ◽

1969 ◽

Vol 74 (3) ◽

pp. 213-219

Author(s):

Maddineni M. Rao ◽

Calixte George

Keyword(s):

Water Treatment ◽

Gibberellic Acid ◽

Dioscorea Alata ◽

Dormancy Period ◽

Harvest Dates ◽

White Yam ◽

And Storage ◽

Early Harvest ◽

Storage Temperatures ◽

Control Water

Two experiments were conducted in 1987-88 in St. Lucia with a view to extend the dormancy of white yam (Dioscorea alata L). Experiment 1 compared different harvest dates, different storage temperatures and presence or absence of the tuber "head" in cultivar Oriental. Experiment 2 studied the effect of gibberellic acid (GA) solution (1000 p/m) on the dormancy of different cultivars of white yam. Tubers from the early harvest (15 November) had the longest dormancy period (over 7 months), which decreased progressively with delay in harvesting. Storing yam tubers at 20 ± 2°C extended the dormancy by over 5 months as compared to storing them at 30 ± 2°C. The presence or absence of the tuber "head" had no effect. The interaction effects due to dates of harvest X storage temperatures and storage temperatures X manipulation were significant. Cultivars of white yam differed significantly in their dormancy period. Treating the harvested tubers with GA (1000 p/m) for 2 hours extended the dormancy by over 4 months as compared to that of the control (water) treatment.

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Effect of Scarification, Seed Storage Temperature, and Relative Humidity on Lupinus havardii Wats. and Lupinus texensis Hook. Seed Germination

HortScience ◽

10.21273/hortsci.40.3.782 ◽

2005 ◽

Vol 40 (3) ◽

pp. 782-785 ◽

Cited By ~ 1

Author(s):

Wayne A. Mackay

Keyword(s):

Relative Humidity ◽

Storage Temperature ◽

Germination Rate ◽

Seed Storage ◽

Significant Decline ◽

Rapid Decline ◽

Seed Moisture Content ◽

C Storage ◽

Seed Moisture ◽

Seed Scarification

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.

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