Role of Relative Humidity in Processing and Storage of Seeds and Assessment of Variability in Storage Behaviour inBrassicaspp. andEruca sativa

The role of relative humidity (RH) while processing and storing seeds ofBrassicaspp. andEruca sativawas investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species ofBrassicawas also evaluated. The samples were stored at40±2°Cin sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour,B. rapaandB. junceawere better performers thanB. napusandEruca sativa.

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Temperature and Relative Humidity Govern Germination and Storage of Gladiolus Seed

HortScience ◽

10.21273/hortsci.26.8.1054 ◽

1991 ◽

Vol 26 (8) ◽

pp. 1054-1057 ◽

Cited By ~ 1

Author(s):

W.J. Carpenter ◽

G.J. Wilfret ◽

J.A. Cornell

Keyword(s):

Relative Humidity ◽

Seed Treatment ◽

No Reduction ◽

Germination Rate ◽

Seed Viability ◽

Seed Storage ◽

Moisture Contents ◽

Seed Moisture ◽

Germination Temperature ◽

And Storage

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.

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Effect of Desiccation and Storage Environment on Longevity of Ehretia Cymosa Thonn. Seeds.

10.21203/rs.3.rs-43618/v1 ◽

2020 ◽

Author(s):

Peter Murithi Angaine ◽

Stephen Muriithi Ndungú ◽

Alice Adongo Onyango ◽

Jesse Omondi Owino

Keyword(s):

Moisture Content ◽

Storage Temperature ◽

Seed Viability ◽

Seed Storage ◽

Storage Duration ◽

Seed Moisture Content ◽

Seed Moisture ◽

Storage Behavior ◽

Storage Studies ◽

And Storage

Abstract Background: Globally, forestry faces challenges in the availability of seeds due to limited knowledge on seed handling of various species. Forestry seeds are constantly being reviewed and classified as either recalcitrant, intermediate, or orthodox based on their storage behavior. It is essential to understand the tree seed storage behavior to maintain seed viability and thus minimize seed losses. There is scanty literature combining factors of seed moisture content (6%, 9%, 12%, 15%, and 20%), seed storage temperature (20oC, 5oC and -20oC), seed storage duration (1, 4, 9 and 12 months), and germination in different sites with varying environmental variables. Ehretia cymosa is important in the Afromontane forestry landscape as a medicinal, rehabilitation, and conservation species. This study conducted desiccation and storage studies and their influence on the viability of E. cymosa seeds. The study sought to determine the optimum conditions for the storage of Ehretia cymosa that maintains viability. Results: This study observed that E. cymosa dried to seed moisture content of 6%, stored for 12 months at 20oC and sown in the laboratory had the highest germination performance (27.6 ± 3.18%) (p<0.05). Conclusion: This confirms that E.cymosa seeds exhibit orthodox storage behavior. The authors recommend longer storage studies (>12months) to determine the actual longevity of the seeds of this species. The significance of these results would be useful for foresters and farmers that would need to use this species for various purposes.

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Effect of Scarification, Seed Storage Temperature, and Relative Humidity on Lupinus havardii Wats. and Lupinus texensis Hook. Seed Germination

HortScience ◽

10.21273/hortsci.40.3.782 ◽

2005 ◽

Vol 40 (3) ◽

pp. 782-785 ◽

Cited By ~ 1

Author(s):

Wayne A. Mackay

Keyword(s):

Relative Humidity ◽

Storage Temperature ◽

Germination Rate ◽

Seed Storage ◽

Significant Decline ◽

Rapid Decline ◽

Seed Moisture Content ◽

C Storage ◽

Seed Moisture ◽

Seed Scarification

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.

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Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka

Jurnal Penelitian Pertanian Tanaman Pangan ◽

10.21082/jpptp.v34n3.2015.p219-227 ◽

2016 ◽

Vol 34 (3) ◽

pp. 219

Author(s):

Ari Wahyuni ◽

M. R. Suhartanto ◽

Abdul Qadir

Keyword(s):

Moisture Content ◽

Initial Moisture Content ◽

Seed Viability ◽

Soybean Seed ◽

Storage Period ◽

Seed Storage ◽

Seed Moisture Content ◽

Seed Moisture ◽

Input Model ◽

Seed Storability

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>

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Evaluation of Temperature and Moisture Content during Storage on the Germination of Flowering Annual Seed

HortScience ◽

10.21273/hortsci.30.5.1003 ◽

1995 ◽

Vol 30 (5) ◽

pp. 1003-1006 ◽

Cited By ~ 4

Author(s):

William J. Carpenter ◽

Eric R. Ostmark ◽

John A. Cornell

Keyword(s):

Relative Humidity ◽

Moisture Content ◽

Seed Storage ◽

Seed Moisture Content ◽

Moisture Contents ◽

Seed Moisture ◽

Storage Temperatures

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.

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Germination and Storage of Vinca Seed Is Influenced by Light, Temperature, and Relative Humidity

HortScience ◽

10.21273/hortsci.27.9.993 ◽

1992 ◽

Vol 27 (9) ◽

pp. 993-996 ◽

Cited By ~ 5

Author(s):

William J. Carpenter ◽

Joseph F. Boucher

Keyword(s):

Relative Humidity ◽

Gibberellic Acid ◽

Catharanthus Roseus ◽

Continuous Light ◽

Seed Storage ◽

Germination Percentage ◽

Seed Moisture ◽

And Storage ◽

Storage Temperatures

Light, temperature, relative humidity (RH), and GA3 affect vinca [Catharanthus roseus (L.) G. Don] seed storage and/or germination. GA3 failed to increase the germination percentage in darkness but significantly increased the percentage in continuous light. Similarly, GA3 treatment reduced both the number of days required to achieve 50% of the final germination percentage (T50) and the span between 10% and 90% germination (T90 — T10) for seeds in light, but not in darkness. Germination percentages were maximal and about equal at 25, 30, or 35C in darkness; germination was lowest below 25C. Germination T50 and T90 — T10 required the fewest days between 25 and 35C. Reducing seed moisture from 9.9% to 3.9% increased the T50 from 2.4 to 3.0 days but failed to change germination percentages. Germination percentage declined linearly as seed storage temperatures were reduced from 5 to — 20C, whereas days to T50 increased. Seed storage for 12 months without reduction in germination percentage was possible at 5C and 11%, 33%, or 52% RH, but storage at 75% or 95% RH for periods exceeding 1 month reduced germination. Seeds stored at 33% or 52% RH required fewer days to T50 than did seeds stored at 11%, 75%, or 95% RH. Chemical name used: gibberellic acid (GA3).

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Conservation of Garcinia imberti Bourd. through seeds

Plant Science Today ◽

10.14719/pst.2019.6.2.518 ◽

2019 ◽

Vol 6 (2) ◽

pp. 243-251

Author(s):

M Anto ◽

M Angala ◽

P S Jothish ◽

C Anilkumar

Keyword(s):

Seed Germination ◽

Relative Humidity ◽

Moisture Content ◽

Gibberellic Acid ◽

Biosphere Reserve ◽

Seed Storage ◽

Seed Moisture Content ◽

Evergreen Forests ◽

Seed Moisture ◽

Subsequent Exposure

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.

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Three-dimensional Models Represent Seed Moisture Content as a Function of Relative Humidity and Temperature

HortScience ◽

10.21273/hortsci.33.7.1207 ◽

1998 ◽

Vol 33 (7) ◽

pp. 1207-1209 ◽

Cited By ~ 5

Author(s):

Jian Fang ◽

Frank Moore ◽

Eric Roos ◽

Christina Walters

Keyword(s):

Relative Humidity ◽

Moisture Content ◽

Citrullus Lanatus ◽

Three Dimensional ◽

Selection Criterion ◽

Seed Storage ◽

Response Surfaces ◽

Seed Moisture Content ◽

Seed Moisture ◽

Three Dimensional Models

Seed moisture content (MC) has been considered the most important factor controlling physiological reactions in seeds, and MC changes with relative humidity (RH) and temperature (T). This relationship is revealed by studying the interaction of RH and T at equilibrium. Cucumber (Cucumis sativus L.), lettuce (Lactuca sativa L.), maize (Zea mays L.), onion (Allium cepa L.), pea (Pisum sativum L.), and watermelon (Citrullus lanatus M. & N.) seeds were equilibrated over sulfuric acid (1% RH) and various saturated salt solutions (5.5% to 93% RH) at temperatures from 5 to 50 °C. Best-fit subset models were selected from the complete third-order model MC = β0 + β1*RH + β2*T + β3*RH2 + β4*T2 + β5*RH*T + β6*RH3 + β7*T3 + β8*RH*T2 + β9*RH2*T, using Mallows' minimum Cp as the selection criterion. All six best subset models (R2, 0.98 to 0.99) had the same functional form, MC = β0 + β1*RH + β2*T + β3*RH2 + β5*RH*T + β6*RH3 + β9*RH2*T. Coefficients had essentially the same respective values among all species except onion and pea, for which some coefficients were statistically different from those of the other species (P ≤ 0.05). All models indicated that seed MC increased as RH increased and decreased as T increased; but RH had the greater influence. The inverse relationship between seed MC and T, although slight, was evident in the response surfaces. The interaction effect of RH and T on MC was significant at P ≤0.001. These results suggest that orthodox seed species respond similarly to T and RH. This in turn suggests that a common model could be developed and used for optimizing seed storage environments.

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Effect of Periods and Media Storage on Viability of Cananga Seed (Cananga odorata (Lam.) Hook.f. & Thomson forma genuina)

Jurnal Sylva Lestari ◽

10.23960/jsl37277-288 ◽

2019 ◽

Vol 7 (3) ◽

pp. 277

Author(s):

Ferawati Oktia Nurhayani ◽

Arum Sekar Wulandari

Keyword(s):

Seed Viability ◽

Seed Storage ◽

Maximum Growth ◽

Germination Percentage ◽

Growth Potential ◽

Seed Moisture Content ◽

Seed Moisture ◽

Significant Difference ◽

Media Storage ◽

Cananga Odorata

The suitable seed storage method is important to maintain seed viability. This study aimed to examine the effect of periods and media storage on the viability of cananga seed (Cananga odorata (Lam.) Hook.f. & Thomson forma genuina). The treatments used in the seed viability test were 0, 2, 4, and 6 weeks as period storage and cocopeat, ash, and charcoal as media storage. The seeds were stored in the plastic at room temperature (± 25°C). The variables observed in this study were seed moisture content, percentage of germination, maximum growth potential, average days of germination, T50, germination value, and the number of seeds that germinate during storage. The percentage of C. odorata seeds germination without storage is 54%. The seed storage for 2 – 6 weeks in ash and charcoal hush caused their viability was drop. The seeds storage of C. odorata up to 6 weeks in cocopeat could maintain seeds viability with a percentage of germination that has no significant difference compared to control. In general, cocopeat can be used as one of the storage media in the seed storage of C. odorata before sowing.Keywords: Cananga odorata, cocopeat, germination percentage, seed, viability

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Temperature and Seed Moisture Govern Germination and Storage of Gerbera Seed

HortScience ◽

10.21273/hortsci.30.1.98 ◽

1995 ◽

Vol 30 (1) ◽

pp. 98-101 ◽

Cited By ~ 4

Author(s):

William J. Carpenter ◽

Eric R. Ostmark ◽

John A. Cornell

Keyword(s):

Relative Humidity ◽

Low Temperature ◽

Seed Storage ◽

Gerbera Jamesonii ◽

Mean Temperature ◽

Seed Moisture ◽

And Storage

Temperature, relative humidity (RH), desiccation, and hydration affect gerbera (Gerbera jamesonii H Bolus ex Hook.f.) seed storage and germination. Germination percentages (G) were maximal and about equal at constant 15, 20, or 25C in darkness or light but lower at alternating temperatures having the same mean temperature. The number of days to 50% final germination (T50) and between 10% and 90% germination (T90 – T10) required the fewest days at constant 25 or 30C; longer germination periods resulted with alternating temperatures. Reducing seed moisture from 7.1% to 3.5% had no effect on G, T50, or T90 – T10 values, but at seed moisture levels <3.5%, G was lower and T50 and T90 – T10 longer. Germination percentages were similar after seed storage from 5 to –5C, but G was lower after storage at –10C or lower. Low-temperature seed storage had no effect on T50 or T90 – T10 values. Seeds had highest G and lowest T50 and T90 – T10 values when germinated at 52% seed moisture, with large declines and delays in germination at lower and higher moisture levels. Seed storage for 12 months without reduction in germination was possible at 5C and 11% or 32% RH. Seeds stored at 52% RH lost G at all temperatures, and no seed germinated after storage at 75% RH and 15 or 25C. Seed stored at 5 or 15C and 11% to 32% RH had the fewest days to T50 and T90 – T10.

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