scholarly journals 878 PB 264 Micropropagation of pitaya (Hylocereus undatus Britt. et Rose)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 559f-559 ◽  
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Distal Part ◽  
Axillary Buds ◽  
Naphthaleneacetic Acid ◽  
Skoog Medium ◽  
Lateral Shoots ◽  
The Family ◽  
Murashige And Skoog Medium ◽  
Hylocereus Undatus ◽  
Number Of Shoots

Pltaya is a member of the family Caztacaae and the genus Hylocerus which has several species producing edible fruits. A procedure for micropropagation of pitaya using thidiazuron is described. Explants were excised from young joints of mature plants, and cultured on Murashige and Skoog medium (MS) containing 0.5 uM Thidiazuron (TDZ), and 3.5 uM naphthaleneacetic acid (NAA) Produced shoots were cut longitudinally into three explants or decapitated and cultured on MS supplemented with 0.01, 0.09, 0.5, or 0.9 uM TDZ with 0.5 uM NAA. Decapitated expiants produced shoots with higher frequency and number of shoots was higher than 1/3 explants. Shoots produced from decapitated explants were longer, thicker and vigorous, compared to shoots developed from 1/3 explants. Most shoots developed from, the distal part in both explants and produced several lateral shoots from axillary buds. Shoots were rooted in MS then transferred to soil and produced normal plants.

scholarly journals Optimation of Auxin and Cytokinin on Enhanced Quality and Weight of Coffea liberica Somatic Embryos

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Fitria Ardiyani ◽  
Edy Setiti Wida Utami ◽  
Hery Purnobasuki
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Dry Weight ◽  
Development Stage ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Coffea Liberica ◽  
Germination Stage

Coffea liberica is a variety of coffee that tolerant to marginal land, especially peatlands. One of propagation methods in C. liberica is somatic embryogenesis(SE) which producing large number of true-to-type plant seedlings in a short time. This research aimed at studying the effect of application of plant growthregulator (PGR) on quality and weight of somatic embryo of C. liberica. Somatic embryo in development stage was induced by Murashige and Skoog medium containing cytokinin as benzyl amino purin (BAP) and auxin as 2,4-dichlorophe-noxyacetic acid (2,4-D). While cotyledonary embryo in germination stage was induced by Murashige and Skoog medium containing cytokinin (BAP) and auxins as 2,4-D, indole acetic acid (IAA) and naphthaleneacetic acid (NAA). The resultsshowed that the application of auxins and cytokinins on development stage affected the formation of embryos, texture of calli, color of calli and embryos, and weight of somatic embryo. It also influenced the shoot and root formation, color and weight of geminating embryos of C. liberica at the germinating stage. During the development stage, addition of 1 mg/L BAP in the absence of 2,4-D in MS medium produced the highest quality of somatic embryo of C. liberica. This medium also produced heaviest somatic embryos but with lighter callus. While in germination stage, all medium treatments produced a typical germinating embryo. Coffea liberica germinating embryo growth optimally on MS medium containing 0.5 mg/L BAP as a single chemical or 0.5 mg/L BAP in combination with 0.5 mg/L IAA for shooting development. Whereas on rooting development, addition of 0.5 mg/L NAA on MS medium produced an optimal germinating embryo. Moreover, germination embryo of C. liberica recorded the highest in terms of dry weight on MS media with addition of 0.5 mg/L BAP. Application of appropriate concentration of auxin and cytokinin is needed to support the formation of somatic embryo and germinating embryo.

scholarly journals In Vitro Micropropagation of 54 Species from 15 Genera of Bamboo

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 453-454 ◽  
Author(s):  
P. Prutpongse ◽  
P. Gavinlertvatana
Keyword(s):  
Room Temperature ◽  
Multiple Shoots ◽  
Axillary Buds ◽  
Skoog Medium ◽  
Stem Node ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Strength Medium

Fifty-four out of 67 species of bamboo tested were successfully propagated in vitro. For nearly every species, multiple shoots were produced from axillary buds on stem node segments cultured on Murashige and Skoog medium containing BA. In a very few species plants could be regenerated adventitiously from callus. This method of propagation was not very efficient or reliable. Rooting occurred in media containing NAA at 2.7 to 5.4 μM. Several species could be stored in vitro on half-strength medium at room temperature > 15 months without transfer. Chemical names used: N6-benzylamino purine (BA); napthyleneacetic acid (NAA).

scholarly journals Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

2001 ◽  
Vol 44 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Marcia O. Mello ◽  
Murilo Melo ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Liquid Medium ◽  
Histological Analysis ◽  
Adventitious Shoots ◽  
Naphthaleneacetic Acid ◽  
Curcuma Zedoaria ◽  
Shoot Bud ◽  
Skoog Medium ◽  
Root Segments ◽  
Murashige And Skoog Medium

Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.

scholarly journals Vegetative propagation of Freesia through the isolation of shoots in vitro.

1976 ◽  
Vol 24 (4) ◽  
pp. 274-277
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans
Keyword(s):  
Vegetative Propagation ◽  
Flower Buds ◽  
Skoog Medium ◽  
Golden Yellow ◽  
Murashige And Skoog Medium ◽  
Number Of Shoots

Shoots from the cvs Ballerina, Aurora, Rijnveld's Golden Yellow and Rose Marie, originating from excised flower-buds, were grown in vitro and were induced to form shoots on a modified Murashige and Skoog medium containing PBA and IAA. The greatest number of new shoots/excised shoot was obtained when the medium contained PBA at 5 mg/l and IAA at 0.1 mg/l. The number of shoots formed depended on the cv. tested. Subcultured shoots, grown on a medium with IAA but without PBA, could be rooted easily and viable plants were obtained. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Germination and Regeneration of Plants from Old Cucumber Seed

HortScience ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 917-918 ◽  
Author(s):  
Nawab Ali ◽  
Robert Skirvin ◽  
Walter E. Splittstoesser ◽  
William L. George
Keyword(s):  
Relative Humidity ◽  
Cucumis Sativus ◽  
Naphthaleneacetic Acid ◽  
Skoog Medium ◽  
Cucumber Seed ◽  
Murashige And Skoog Medium

Cucumber (Cucumis sativus L.) seeds of `Marketer', `Marketmore', `Wisconsin SMR-18', `Tablegreen', `Spotfree', and `China' were stored at 3C and 38% relative humidity for up to 26 years. Seed older than 13 years did not germinate. Cultivars stored 10 years gave 80% germination, except Wisconsin SMR-18' (40%). Ten-year-old seeds were separated from their seedcoats, and cotyledons were excised into six segments. Explants were placed on Murashige and Skoog medium with all combinations of BAP (0, 1,2, and 3 mg·liter-1) and NAA (0, 0.1,0.2, and 0.3 mg·liter-1). Plants were obtained from culture for all cultivars grown on medium containing NAA and 1 mg BAP/liter. No plants were regenerated when BAP or NAA was lacking. Chemical names used: benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA).

scholarly journals 880 PB 270 In Vitro Flower and Plant Formation from Opuntia dillenii Haw. and Opuntia cochenillifera Mill.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560a-560
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Fresh Medium ◽  
Naphthaleneacetic Acid ◽  
Forage Crop ◽  
Skoog Medium ◽  
Number Of Species ◽  
Murashige And Skoog Medium ◽  
Opuntia Dillenii

The genus Opuntia includes a number of species which produce nutritious fruits and edible young joints, moreover, they are used as forage crop and for medicinal purposes. A procedure for flower and plant formation is described. Explants were excised from young flower receptacle and cultured on Murashige and Skoog medium (MS) supplemented with 0.9, 1.8, 4.5, or 9 uM thidiazuron and 0.5 uM naphthaleneacetic acid. Produced shoots were bisected longitudinally and cultured in a fresh medium from the same composition, other half shoots were subjected to a second cut by dividing transversely into distal and proximal explants before culture. About 80% explants of O. dellini produced flowers which produced shoots form the distal ales. only shoots were formed from O. cochenillifera explants. The distal explants produced more shoots than proximal explants. All formed shoots which attained 3 mm in length or longer were rooted directly in soil and all shoots formed roots and produced normal plants.

Plantlet production from anthers of Eastern cottonwood (Populusdeltoïdes)

1988 ◽  
Vol 18 (7) ◽  
pp. 937-941 ◽  
Author(s):  
M. Rafique Uddin ◽  
Martin M. Meyer Jr. ◽  
J. J. Jokela
Keyword(s):  
Butyric Acid ◽  
Woody Plant ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Eastern Cottonwood ◽  
Skoog Medium ◽  
Plantlet Production ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
2,4 Dichlorophenoxyacetic Acid

Plantlets were obtained by organogenesis from cultured anthers of Populusdeltoides (Bartr.). Anthers formed callus in the dark on modified Murashige and Skoog medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid and 4.7 μM kinetin. Anther calli were differentiated into shoots by sequential transfer in the light onto Murashige and Skoog medium containing 4.4 μM benzylamino purine and 1.1 μM naphthaleneacetic acid for 4 weeks, followed by several transfers to woody plant medium with 2.2 μM benzylamino purine and 1.1 μM naphthaleneacetic acid. The shoots that formed were rooted by excising and transferring to woody plant medium supplemented with 1.0 μM indole-3-butyric acid. A few of these plants were found to be haploid. Two plants developed male terminal inflorescences, but died shortly thereafter.

scholarly journals Tissue Culture Propagation of Yam in Puerto Rico

1969 ◽  
Vol 67 (4) ◽  
pp. 419-428
Author(s):  
Amelia Cortés-Monllor ◽  
Lii-Jang Liu
Keyword(s):  
Tissue Culture ◽  
Puerto Rico ◽  
Indoleacetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Dioscorea Rotundata ◽  
Skoog Medium ◽  
Rooting Medium ◽  
Murashige And Skoog Medium ◽  
Tissue Culture Propagation

Rapid propagation of yam (Dioscorea rotundata cv. Habanero) was obtained from nodal segments cultured in modified Murashige and Skoog medium containing indoleacetic acid (IAA) and kinetin as establishment medium, and naphthaleneacetic acid (NAA) as rooting medium. The proliferation cycle, which takes approximately 2-3 months, increased four times the production of yam plantlets. These plantlets were successfully transferred to potting mixture and soil. This procedure is extremely useful for regenerating virusfree plantlets suitable for producing healthy tubers for planting.

scholarly journals 879 PB 267 Micropropagation of Grand Crinum Lily (Crinum asiaticum L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 559g-559
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Multiple Shoots ◽  
Ornamental Plant ◽  
White Flower ◽  
Fresh Medium ◽  
Naphthaleneacetic Acid ◽  
Plant Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Crinum Asiaticum

Grand crinum Lily is an ornamental plant which is considered to be one of the architect's favorite accent. plants and one that bloom most of the year. A protocol for plant propagation from inflorescence is described. Explants were excised from inflorescence at the primordial stage. and cultured on Murashige and Skoog medium (MS: alone or supplemented with benzyladenine 4.4 or 13.3 uM benzyladenine (BA) and 0.5 uM naphthaleneacetic acid (NAA) and incubated for four weeks. Explants cultured on BA-containing media produced white flower-like structures on the receptacle which produced multiple shoots after additional four weeks on a fresh medium containing 4.4 uM BA and 0.05 uM NAA. Shoots were transferred to a fresh medium for further growth during which the basal stem reached 3 to 5 mm diameter. At this stage shoots, with or without roots, were transferred to soil, without acclimatization, and normal plants were established in soil.

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