scholarly journals Physiological, Biochemical, and Molecular Changes in Pelargonium Cuttings Subjected to Short-term Storage Conditions

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 637a-637
Author(s):  
Richard N. Arteca ◽  
Jeannette M. Arteca ◽  
Tzann-Wei Wang ◽  
Carl D. Schlagnhaufer
Keyword(s):  
Gene Expression ◽  
Acc Oxidase ◽  
Storage Conditions ◽  
Sharp Decline ◽  
Short Term ◽  
Molecular Changes ◽  
Visual Rating ◽  
Shoot Weight ◽  
Term Storage ◽  
Over Time

The purpose of this study was to evaluate physiological, biochemical and molecular changes which occur in unrooted Pelargonium ×hortorum cuttings during storage. Pelargonium cuttings of `Sincerity' (good shipper), `Wendy Ann' (moderate shipper), and `Snowmass' (poor shipper) were stored at 25°C and evaluated over a 5-day period. Following removal from storage, cuttings exhibited progressive declines in photosynthesis, respiration, carbohydrate, starch and protein over time which was significant in all three cultivars, however there was little difference among the cultivars. Ethylene levels produced by `Sincerity' and `Wendy Ann' began to increase 3 days following the initiation of storage, whereas `Snowmass' showed an increase after one day reaching a peak at 3 days and was followed by a sharp decline. When unrooted cuttings of `Snowmass' were stored for a 5-day period at temperatures from 4 to 25°C, it was observed that those stored at 4°C had a significantly higher visual rating, chlorophyll content, root and shoot weight than at higher temperatures tested. The decline in quality progressively became greater from 10 to 25°C. Changes in gene expression of two ACC synthases and an ACC oxidase were evaluated in `Snowmass' cuttings which were stored at 4 and 25°C. Correlations between ethylene and ACC levels with gene expression were observed.

scholarly journals Physiological, Biochemical, and Molecular Changes in Pelargonium Cuttings Subjected to Short-term Storage Conditions

1996 ◽  
Vol 121 (6) ◽  
pp. 1063-1068 ◽  
Author(s):  
Richard N. Arteca ◽  
Jeannette M. Arteca ◽  
Tzann-Wei Wang ◽  
Carl D. Schlagnhaufer
Keyword(s):  
Gene Expression ◽  
Acc Oxidase ◽  
Storage Conditions ◽  
Significant Decline ◽  
Short Term ◽  
Molecular Changes ◽  
Visual Rating ◽  
Shoot Weight ◽  
Term Storage

The purpose of this study was to evaluate physiological, biochemical, and molecular changes that occur in unrooted Pelargonium ×hortorum cuttings during storage. Pelargonium cuttings of `Sincerity' (good shipper), `Wendy Ann' (moderate shipper) and `Snowmass' (poor shipper) were stored at 25 °C and evaluated over a 5-day period. Following removal from storage, cuttings of all cultivars exhibited steady and significant decline in photosynthesis, respiration, carbohydrate, starch, and protein over time. However, no significant differences were observed among cultivars for all of these parameters. Ethylene levels produced by `Sincerity' and `Wendy Ann' began to increase 3 days following storage; whereas, `Snowmass' showed an increase after 1 day, reaching a peak at 3 days, and then declined. When unrooted cuttings of `Snowmass' were stored for 5 days at temperatures ranging from 4 to 25 °C, it was observed that those stored at 4 °C had a significantly higher visual rating, chlorophyll content, and root and shoot weight than at higher temperatures tested. As temperature increased from 10 to 25 °C, quality of cuttings declined. Changes in gene expression of two ACC synthases and an ACC oxidase were evaluated in `Snowmass' cuttings stored at 4 and 25 °C. Correlations between ethylene and ACC levels with gene expression were observed. Chemical name used: 1-aminocyclopropane-1-carboxylic acid (ACC).

scholarly journals Impact of Thermotherapy and Short-Term Storage on Quercus robur L. Acorn Mycobiota and Germination

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 528
Author(s):  
Jelena Kranjec Orlović ◽  
Damir Drvodelić ◽  
Marko Vukelić ◽  
Matea Rukavina ◽  
Danko Diminić ◽  
...  
Keyword(s):  
Natural Regeneration ◽  
Fungal Diversity ◽  
Quercus Robur ◽  
Negative Impact ◽  
Storage Conditions ◽  
Short Term ◽  
Isolation Frequency ◽  
Short Term Storage ◽  
Penicillium Spp ◽  
Term Storage

When natural regeneration of Quercus robur stands is hampered by an insufficient acorn yield, human assisted sowing of acorns collected in non-affected stands and stored for some period of time is performed. To inhibit the development of fungi and acorn deterioration during storage, thermotherapy is usually applied by submerging acorns for 2.5 h in water heated to 41 °C. This research aimed to test the effect of four thermotherapy treatments of different durations and/or applied temperatures as well as short-term storage at −1 °C or 3 °C on acorn internal mycobiota and germination. Fungal presence in cotyledons was analyzed in 450 acorns by isolation of mycelia on artificial media, followed by a DNA-based identification. Germination of 2000 acorns was monitored in an open field trial. Thermotherapy significantly decreased fungal diversity, while storage at 3 °C increased the isolation frequency of several fungi, mainly Penicillium spp. The most frequently isolated fungi did not show a negative impact on acorn germination after short-term storage. The study confirmed the efficiency of thermotherapy in the eradication of a part of acorn internal mycobiota, but also its effect on the proliferation of fast-colonizing fungi during storage. However, the latter showed to be more stimulated by storage conditions, specifically by storage at 3 °C.

scholarly journals Properties of Fiberboard Overpack Material in the 9975 Shipping Package Following Thermal Aging

2007 ◽  
Author(s):  
W. L. Daugherty
Keyword(s):  
Service Life ◽  
Physical Properties ◽  
Thermal Aging ◽  
Impact Resistance ◽  
Storage Conditions ◽  
Radioactive Material ◽  
Long Term Storage ◽  
Term Storage ◽  
Over Time

Many radioactive material shipping packages incorporate cane fiberboard overpacks for thermal insulation and impact resistance. Mechanical, thermal and physical properties have been measured on cane fiberboard following thermal aging in several temperature/humidity environments. Several of the measured properties change significantly over time in the more severe environments, while other properties are relatively constant. These properties continue to be tracked, with the goal of developing a model for predicting a service life under long-term storage conditions.

Use of a Maggot Motility Index to Evaluate Survival of Therapeutic Larvae

2004 ◽  
Vol 94 (4) ◽  
pp. 353-355 ◽  
Author(s):  
Anthony Rosales ◽  
Jefferey R. Vazquez ◽  
Brian Short ◽  
Heather R. Kimbriel ◽  
Matthew J. Claxton ◽  
...  
Keyword(s):  
Room Temperature ◽  
Wound Care ◽  
In Vivo Studies ◽  
Clinical Use ◽  
Short Term ◽  
Motility Index ◽  
Term Storage ◽  
Over Time ◽  
Surrogate Method

Maggot debridement therapy is rapidly increasing in popularity at major diabetic foot and wound care centers worldwide. However, we are unaware of specific guidelines on the short-term storage of larvae. We sought to evaluate differences in maggot motility over time in larvae refrigerated versus those stored at room temperature. We also introduce a simple surrogate method for evaluating maggot vitality that may be useful for in vivo studies if validated in future works. We randomly selected ten larvae from the same shipment at ten different times in 9 days. Larvae were placed on a translucent acetate grid, and their total excursion in 30 sec was measured. This was converted into a Maggot Motility Index. In the refrigerated group, the index remained at or above 40 mm/min for approximately 60 hours from baseline, when there was a significant decrease. This same phenomenon occurred during the first 12 hours in the nonrefrigerated group. There were significant differences in motility between refrigerated and nonrefrigerated larvae immediately after baseline until day 8. Larvae are more practical for repeated clinical use if kept refrigerated between applications. (J Am Podiatr Med Assoc 94(4): 353–355, 2004)

scholarly journals Management of Apple Maturity and Postharvest Storage Conditions to Increase Polyphenols in Cider

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart
Keyword(s):  
The United States ◽  
Storage Conditions ◽  
Short Term ◽  
Postharvest Storage ◽  
Total Polyphenols ◽  
Fruit Storage ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

Preanalytical mRNA Stabilization of Whole Bone Marrow Samples.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4407-4407
Author(s):  
Claudia Langebrake ◽  
Kalle Guenther ◽  
Juergen Lauber ◽  
Dirk Reinhardt
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Rna Isolation ◽  
Isolation Procedure ◽  
Short Term ◽  
Expression Levels ◽  
Mrna Stabilization ◽  
Short Term Storage ◽  
Term Storage ◽  
Gene Expression Levels

Abstract Background: Gene expression profiling is a useful tool for the diagnosis and basic research of cancer. One of the major limitations of this approach is that already a short-term storage of native specimens of peripheral blood (PB) or bone marrow (BM) and/or the method of RNA isolation has significant influence on gene expression due to gene induction, repression and RNA degradation. The objective of the current study was to investigate the influence of a newly developed RNA stabilization and preparation system for BM specimens (PAXgeneTM Bone Marrow RNA System), focusing on RNA-yield, RNA-integrity and stability of gene expression profiles over time of BM sample storage. Patients and methods: 180 RNA samples that have been processed from 45 heparinized BM specimens of unselected children with acute leukemia at initial diagnosis, during treatment or without pathological findings in hematopoiesis were analyzed. Immediately after collection, heparinized BM specimens were divided into two PAXgeneTM RNA tubes and two standard tubes, each. RNA isolation using either the PAXgeneTM protocol (P) or a reference protocol (R) was performed after 2 hours (P0 and R0) or after 48 hours (P2 and R2). Results: The overall RNA yield (normalized to 1×107 leukocytes) was not different in all four isolation procedures, however the integrity of the RNA using the PAXgeneTM system was significantly higher at both time-points than that of the reference system: 8.6±0.2 (P0) vs. 6.8±0.4 (R0), p=0.0003 and 8.1±0.2 (P2) vs. 6.7±0.5 (R2), p=0.008. For the stabilized samples, we found very good pairwise correlation in gene expression for either gene at P2 compared to P0: GATA1 89±6%, RUNX1 83±10%, NCAM 69±12% and SPI1 89±6%. However, there were significant differences in two of the analyzed genes using the reference RNA isolation procedure: 40±6%, p<0.001 (GATA1) and 47±8%, p=0.005 (NCAM), respectively. Comparing the two RNA isolation procedures, there are significant differences in the expression levels of the analyzed genes. Discussion: The PAXgeneTM system is appropriate for the stabilization of gene expression levels in BM samples after short-term storage. However, as in some genes the reference RNA isolation procedure resulted in similar gene expression levels, the use of a stabilization reagent has to be carefully evaluated within the preanalytical handling of the samples. Our analysis has shown that it is not suitable to compare RNA expression levels derived from different isolation procedures. In conclusion, the PAXgeneTM system is able to stabilize RNA from clinical BM samples while being suitable to isolate high quality and quantity RNA.

scholarly journals Evaluation of sweet cherry fruit quality after short-term storage in relation to the rootstock

2019 ◽  
Vol 60 (6) ◽  
pp. 925-934 ◽  
Author(s):  
Ewa Dziedzic ◽  
Jan Błaszczyk
Keyword(s):  
Fruit Quality ◽  
Sweet Cherry ◽  
Fungal Infections ◽  
Weather Conditions ◽  
Storage Conditions ◽  
Soluble Solids ◽  
Short Term ◽  
Fruit Storage ◽  
Short Term Storage ◽  
Term Storage

Abstract Fruits of the sweet cherry cultivar ‘Regina’ collected from trees growing on seven rootstocks were stored in a cold room at 2 °C with a normal (NA) and controlled atmosphere (15% and 20% CO2 and 5% O2—CA1 and CA2) for 2 weeks. The rootstocks on which the trees grew and the storage conditions significantly affected all fruit parameters tested during both years of the experiment. Fruit from Damil rootstock exhibited higher mean firmness than fruit from Colt rootstock. The effect of rootstocks on the value of soluble solids content (SSC) varied, wherein the fruits from Tabel Edabriz and Damil were characterized by high SSC mean content. The organic acids content (TA) was significantly lower after storage than during harvest time. Fruits from Tabel Edabriz trees were characterized by faster ripening, as was evident by the higher SSC to TA ratio. The amount of mass lost depended significantly and only on the storage conditions—sweet cherries from both CA combinations had the lowest mass losses. The percentage of fruits showing disease symptoms was largely dependent on the weather conditions in the orchard the year before the fruit harvest, as well as atmosphere composition and RH during fruit storage. Cold storage conditions with a high (20%) CO2 content are recommended for the short-term storage of sweet cherry fruits because they preserve fruit quality parameters: a low decrease in firmness, maintenance of a high SSC/TA ratio, a low percent of fungal infections, and good preservation of green color in the peduncle.

scholarly journals Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11933
Author(s):  
Adrian Simpson ◽  
Aaron Topol ◽  
Bradley J. White ◽  
Marlene K. Wolfe ◽  
Krista R. Wigginton ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Storage Conditions ◽  
Mottle Virus ◽  
Surveillance Program ◽  
Short Term ◽  
Pepper Mild Mottle Virus ◽  
Strong Interest ◽  
Rna Quantification ◽  
Term Storage

SARS-CoV-2 RNA in wastewater settled solids is associated with COVID-19 incidence in sewersheds and therefore, there is a strong interest in using these measurements to augment traditional disease surveillance methods. A wastewater surveillance program should provide rapid turn around for sample measurements (ideally within 24 hours), but storage of samples is necessary for a variety of reasons including biobanking. Here we investigate how storage of wastewater solids at 4 °C, −20 °C, and −80 °C affects measured concentrations of SARS-CoV-2 RNA. We find that short term (7 or 8 d) storage of raw solids at 4 °C has little effect on measured concentrations of SARS-CoV-2 RNA, whereas longer term storage at 4 °C (35–122 d) or freezing reduces measurements by 60%, on average. We show that normalizing SARS-CoV-2 RNA concentrations by concentrations of pepper mild mottle virus (PMMoV) RNA, an endogenous wastewater virus, can correct for changes during storage as storage can have a similar effect on PMMoV RNA as on SARS-CoV-2 RNA. The reductions in SARS-CoV-2 RNA in solids during freeze thaws is less than those reported for the same target in liquid influent by several authors.

scholarly journals Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 806
Author(s):  
Aitor Fernandez-Novo ◽  
Sergio Santos-Lopez ◽  
Clara Barrajon-Masa ◽  
Patricia Mozas ◽  
Eduardo de Mercado ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Room Temperature ◽  
Storage Time ◽  
Storage Conditions ◽  
Short Term ◽  
Bull Semen ◽  
The Impact ◽  
Term Storage ◽  
Diagnostic Centre ◽  
Holding Temperature

CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 °C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 °C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.

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