scholarly journals 492 Storage of Abelia R. Br. Pollen

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 530A-530
Author(s):  
S.M. Scheiber ◽  
C.D. Robacker ◽  
M.A. Dirr
Keyword(s):  
Plant Material ◽  
Cold Hardiness ◽  
Pollen Viability ◽  
Pollen Storage ◽  
Limited Access ◽  
Long Term Storage ◽  
Temperature And Humidity ◽  
Term Storage

The genus Abelia contains ≈30 species, but A. × grandiflora, its cultivars, and A. `Edward Goucher' are the primary taxa grown. The nursery industry has stated that Abelia R. Br. taxa are important economically, and new selections or cultivars with increased cold hardiness, richer pink-rose flower colors, unique foliage colors, and compact habits are desired. Breeding and selection work in the genus is very limited due in part to limited access to germplasm. Pollen storage enables breeders to cross taxa with incongruent flowering cycles, save time and resources by eliminating the need to grow vast amounts of plant material, and incorporate otherwise unavailable germplasm into a breeding program. An experiment was conducted to determine the optimum levels of temperature and humidity for the long-term storage of A. chinensis and A. × grandiflora `Golden Glow' pollen. Temperature and humidity levels were analyzed by incubating undesiccated pollen of a given taxon at four humidity levels (0%, 50%, 80%, and 100%) for 72 h at 5 °C. Following incubation, the pollen was stored in glass vials at each of the following temperatures: 5, -20, and -70 °C. All combinations of temperature and humidity were tested. Pollen viability was assessed after 60 days by in vivo germination tests on styles. Abelia chinensis pollen germinated following storage at all temperature and humidity levels. Pollen of A. × grandiflora `Golden Glow' pollen germinated following all treatments except storage at -20 °C.

scholarly journals Does long-term storage of clay samples influence their mechanical characteristics?

2020 ◽  
Vol 57 (2) ◽  
pp. 304-309 ◽  
Author(s):  
Mustapha Abdellaziz ◽  
Mahmoud N. Hussien ◽  
Mohamed Chekired ◽  
Mourad Karray
Keyword(s):  
Mechanical Characteristics ◽  
Long Term Storage ◽  
Temperature And Humidity ◽  
Before And After ◽  
Prime Objective ◽  
Term Storage ◽  
Physical And Mechanical Characteristics

The prime objective of this study is to assess the influence of long-term storage on the physical and mechanical characteristics of clay samples. Samples from two different clays were sealed and stored in a temperature- and humidity-controlled room at the geotechnical laboratory of the Université de Sherbrooke for up to 27 years. The stored clay samples were tested before and after long-term storage and the results compared in this note. The comparison showed that even with long-term storage, the majority of the physical and mechanical characteristics of the samples were preserved.

Stability analysis for long-term storage of naked DNA: impact on nonviral in vivo gene transfer

2003 ◽  
Vol 318 (2) ◽  
pp. 230-235 ◽  
Author(s):  
Wolfgang Walther ◽  
Ulrike Stein ◽  
Carsten Voss ◽  
Torsten Schmidt ◽  
Martin Schleef ◽  
...  
Keyword(s):  
Stability Analysis ◽  
Gene Transfer ◽  
Naked Dna ◽  
Long Term Storage ◽  
Term Storage ◽  
In Vivo Gene Transfer

scholarly journals Development and optimization of a germination assay and long-term storage for Cannabis sativa pollen

2020 ◽  
Author(s):  
Daniel Gaudet ◽  
Narendra Singh Yadav ◽  
Aleksei Sorokin ◽  
Andrii Bilichak ◽  
Igor Kovalchuk
Keyword(s):  
Cannabis Sativa ◽  
Pollen Viability ◽  
Growth Stages ◽  
Dapi Staining ◽  
Long Term Storage ◽  
Whole Wheat ◽  
Germination Assay ◽  
And Storage ◽  
Term Storage

AbstractPollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of Cannabis sativa pollen. Additionally, we have deciphered the bicellular nature of cannabis pollen using DAPI staining. We have also standardized a pollen germination assay to assess the viability of cannabis pollen, and found pollen collected from different principal growth stages exhibits different longevity. Finally, we developed a long-term storage method which includes pollen combination with baked whole wheat flower and desiccation under vacuum for cryopreservation. By using this method, we were able to maintain germination viability in liquid nitrogen after 4 months, suggesting potentially indefinite preservation of cannabis pollen.

Long-term (35 years) cryopreservation of Echinococcus multilocularis metacestodes

Parasitology ◽  
2020 ◽  
Vol 147 (9) ◽  
pp. 1048-1054
Author(s):  
Teivi Laurimäe ◽  
Philipp A. Kronenberg ◽  
Cristian A. Alvarez Rojas ◽  
Theodor W. Ramp ◽  
Johannes Eckert ◽  
...  
Keyword(s):  
Echinococcus Multilocularis ◽  
Alveolar Echinococcosis ◽  
Geographic Origin ◽  
Experimental Animals ◽  
Long Term Storage ◽  
Term Maintenance ◽  
Term Storage

AbstractThe metacestode of Echinococcus multilocularis is the etiological agent of alveolar echinococcosis. The metacestode stage used for research is maintained in rodents by serial passages. In order to determine whether cryopreservation of E. multilocularis metacestodes would be suitable for long-term maintenance and replace serial passages, isolates of different geographic origin were cryopreserved in 1984–1986. The aim of the current study was to test the viability of cryopreserved isolates following long-term cryopreservation (up to 35 years) and to determine the phylogenetic clades these isolates belonged to. Cryopreserved isolates were tested for viability in vitro and in vivo in gerbils. In vitro results of 5 isolates indicated protoscolex survival in 13 of 17 experiments (76%) and metacestode survival in 5 of 12 (42%) in vivo experiments. In vivo results showed ‘abortive lesions’ in 13 of the 36 animals, 15 were negative and 8 harboured proliferating metacestode tissue containing protoscoleces. Genetic analysis confirmed the isolates belonged to European, Asian and North-American clades. In conclusion, the results of the current study indicate that metacestodes of E. multilocularis are able to survive long-term cryopreservation. Therefore, cryopreservation is a suitable method for long-term storage of E. multilocularis metacestode isolates and reduces the number of experimental animals.

Nucleic acid nanocarriers can be programmable, spatially adjustable and biocompatible, minimizing systemic toxicity and improving pharmacodynamics

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Nucleic Acid ◽  
Systemic Toxicity ◽  
Acid Therapy ◽  
Immune Reactions ◽  
Long Term Storage ◽  
Recent Developments ◽  
Functional Components ◽  
Term Storage

This review emphasized recent developments in gene therapy with rationally created nucleic acid nanocarriers. Antisense, RNAi, gene editing, and gene expression therapeutic techniques were highlighted. Structurally programmable, spatially adjustable and biocompatible nucleic acid nanostructures have been evolved as superior loading vehicles for homologous nucleic acid treatments such as antisense, siRNA, shRNA, sgRNA, linear genes, and mRNA. Multiple functional components, including active targeting groups and stimulant-responsive elements, may also be readily inserted into nucleic acid nanocarriers for targeted distribution and controlled release of nucleic acid drugs, helping to minimize systemic toxicity and improve pharmacodynamics. Until recently, the practical application of nucleic acid nanocarriers for the delivery of in vivo nucleic acid therapy remained uncertain. First, the systemic pharmacokinetics of nucleic acid nanostructures, including circulation, distribution, metabolism, and excretion, should be carefully investigated. Second, additional study is needed on the actual cellular internalization processes and intracellular fate of nucleic acid nanostructures of diverse shapes and sizes. Lastly, unmethylated CpG motifs and dsRNA can activate innate immune activation. Fortunately, improving nucleic acid sequences can dramatically decrease immune reactions to nucleic acid nanostructures. Uchida and coworkers observed, for example, that lowering the hybridization length to 17 nt can suppress immune reactions caused by dsRNA. Despite the foregoing constraints, developed nucleic acid nanostructures with different benefits are expected to act as intelligent delivery carriers for gene-related medicines. The nucleic acid nanocarriers have not yet been evaluated in the clinic due to long-term storage and fabrication costs. Many lyophilization-based techniques, including cryopreservation, have been studied for the long-term storage of nucleic acid molecules. Moreover, as biotechnology improves in nucleic acid mass manufacturing, the cost of nucleic acid nanocarriers is projected to drop considerably. According to our projections, preclinical and clinical research based on nucleic acid nanostructures is predicted to begin soon.

Process of Drying Post-Harvest Hops (Humulus lupulus) for Small-Scale Producers Using a Novel Drying Rig

EDIS ◽  
2019 ◽  
Vol 2019 (1) ◽  
Author(s):  
Sean M. Campbell ◽  
Brian J. Pearson
Keyword(s):  
Plant Material ◽  
Humulus Lupulus ◽  
The State ◽  
Small Scale ◽  
Post Harvest ◽  
Long Term Storage ◽  
Term Storage

Fresh horticultural goods often require drying post-harvest to preserve quality and allow for successful long-term storage of plant material. Given the influx of hops cultivation in the state of Florida, this 5-page publication will help Florida hops growers and hobby brewers to understand how to efficiently dry hops prior to storage. Written by Sean Campbell and Brian Pearson and published by the UF/IFAS Environmental Horticulture Department, January 2019. http://edis.ifas.ufl.edu/ep568

Liposome Encapsulated Hemoglobin: Long-Term Storage Stability and in Vivo Characterization

1992 ◽  
Vol 20 (2-4) ◽  
pp. 619-626 ◽  
Author(s):  
Richard O. Cliff ◽  
Frances Ligler ◽  
Beth Goins ◽  
Peter M. Hoffmann ◽  
Helmut Spielberg ◽  
...  
Keyword(s):  
Storage Stability ◽  
Long Term Storage ◽  
In Vivo Characterization ◽  
Term Storage

scholarly journals Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1657
Author(s):  
Samah Shanwar ◽  
Liuen Liang ◽  
Andrey V. Nechaev ◽  
Daria K. Bausheva ◽  
Irina V. Balalaeva ◽  
...  
Keyword(s):  
Colloidal Stability ◽  
Protein Corona ◽  
Systemic Circulation ◽  
Upconversion Nanoparticles ◽  
Long Term Storage ◽  
Theranostic Applications ◽  
Term Storage

In the natural fluidic environment of a biological system, nanoparticles swiftly adsorb plasma proteins on their surface forming a “protein corona”, which profoundly and often adversely affects their residence in the systemic circulation in vivo and their interaction with cells in vitro. It has been recognized that preformation of a protein corona under controlled conditions ameliorates the protein corona effects, including colloidal stability in serum solutions. We report on the investigation of the stabilizing effects of a denatured bovine serum albumin (dBSA) protein corona formed on the surface of upconversion nanoparticles (UCNPs). UCNPs were chosen as a nanoparticle model due to their unique photoluminescent properties suitable for background-free biological imaging and sensing. UCNP surface was modified with nitrosonium tetrafluoroborate (NOBF4) to render it hydrophilic. UCNP-NOBF4 nanoparticles were incubated in dBSA solution to form a dBSA corona followed up by lyophilization. As produced dBSA-UCNP-NOBF4 demonstrated high photoluminescence brightness, sustained colloidal stability after long-term storage and the reduced level of serum protein surface adsorption. These results show promise of dBSA-based nanoparticle pretreatment to improve the amiability to biological environments towards theranostic applications.

scholarly journals Storage and Viability Testing of Protea Pollen

1996 ◽  
Vol 121 (5) ◽  
pp. 804-809 ◽  
Author(s):  
I. David van der Walt ◽  
Gail M. Littlejohn
Keyword(s):  
Storage Temperature ◽  
Pollen Viability ◽  
Germination Percentage ◽  
Long Term Storage ◽  
Fresh Pollen ◽  
The Relationship ◽  
Term Storage ◽  
Storage Temperatures

The influence of storage temperature and humidity on pollen viability was studied in four Protea species. Pollen was stored at a range of temperatures and relative humidities for up to 1 year and tested for ability to germinate in vitro. Pollen of P. repens (L.) L. `Sneyd', P. eximia (Salisb. ex Knight) Fourcade `Fiery Duchess' and P. magnifica Link. clone T 84 07 05 stored at -196 °C and -14 to -18 °C retained a germination percentage as high as that of fresh pollen regardless of humidity. Humidity control became increasingly important at storage temperatures above 0 °C. The study showed that long-term storage of Protea pollen is not feasible at temperatures above 0 °C. The relationship between germinability and fluorochromasia (FCR) was studied during storage of `Sneyd' pollen. The correlations between FCR and germinability were found to be low and nonsignificant. Fifteen-month-old cryopreserved `Sneyd' pollen functioned in fertilization and seed set as effectively as fresh pollen.

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