scholarly journals Leaf Color Stability during Plant Development as an Index of Leaf Color Variation among Micropropagated Caladium

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 328-332 ◽  
Author(s):  
Eakhlas U. Ahmed ◽  
Takahiro Hayashi ◽  
Susumu Yazawa

The developmental pattern of leaf color distribution during plant development in 10 cultivars of Caladium ×hortulanum Birdsey was investigated. We used the color occupying the largest area in the terminal leaf as the dominant color, and expressed the leaf color stability during plant development by the ratio of the percentage of the dominant color area in the terminal leaf to that of the dominant color area in the initial leaf (leaf color stability index). In some cultivars, leaf color stability index was clearly greater than 1 (leaf-color-unstable cultivar), but in some cultivars it was close to 1 (leaf-color-stable cultivar). In plants regenerated from leaf explants of leaf-color-unstable cultivars, many (21% to 43%) color variants were observed but only a few (0% to 6%) occurred from leaf explants of leaf-color-stable cultivars. Tissue culture appears to be a useful technique for rapid propagation based on leaf color stability in leaf-color-stable and leaf-color-unstable cultivars.

2015 ◽  
Vol 14 (4) ◽  
pp. 11827-11840 ◽  
Author(s):  
H.Y. Yang ◽  
X.W. Xia ◽  
W. Fang ◽  
Y. Fu ◽  
M.M. An ◽  
...  

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mingyue Fu ◽  
Zhongcheng Zhou ◽  
Xu Yang ◽  
Zhongbing Liu ◽  
Jiarui Zheng ◽  
...  

Abstract Background Leaf color variation is a common trait in plants and widely distributed in many plants. In this study, a leaf color mutation in Camellia japonica (cultivar named as Maguxianzi, M) was used as material, and the mechanism of leaf color variation was revealed by physiological, cytological, transcriptome and microbiome analyses. Results The yellowing C. japonica (M) exhibits lower pigment content than its parent (cultivar named as Huafurong, H), especially chlorophyll (Chl) and carotenoid, and leaves of M have weaker photosynthesis. Subsequently, the results of transmission electron microscopy(TEM) exhibited that M chloroplast was accompanied by broken thylakoid membrane, degraded thylakoid grana, and filled with many vesicles. Furthermore, comparative transcriptome sequencing identified 3,298 differentially expressed genes (DEGs). KEGG annotation analysis results showed that 69 significantly enriched DEGs were involved in Chl biosynthesis, carotenoid biosynthesis, photosynthesis, and plant-pathogen interaction. On this basis, we sequenced the microbial diversity of the H and M leaves. The sequencing results suggested that the abundance of Didymella in the M leaves was significantly higher than that in the H leaves, which meant that M leaves might be infected by Didymella. Conclusions Therefore, we speculated that Didymella infected M leaves while reduced Chl and carotenoid content by damaging chloroplast structures, and altered the intensity of photosynthesis, thereby causing the leaf yellowing phenomenon of C. japonica (M). This research will provide new insights into the leaf color variation mechanism and lay a theoretical foundation for plant breeding and molecular markers.


2018 ◽  
Vol 5 (4) ◽  
pp. 76
Author(s):  
Mone Laiz Bortoli ◽  
Cristina Von Appen ◽  
Camila Longoni ◽  
Carmen Beatriz Borges Fortes ◽  
Jefferson Tomio Sanada

Aim: This work aimed to evaluate the color stability of an acrylic resin chemically activated (ARCA) using different handling techniques, and a bisacrylic resin when exposed to different pigmentation solutions.Material and Methods: Silicon matrixes were confectioned (10x10x3mm) to be used as specimens. The groups were designed as follows: Group Pot, Group Brush, Group Manufacturer and Group Bisacrylic (n=18). Each group was exposed to three different pigmentation solutions: distilled water, coke and chlorhexidine digluconate 0.12%. Three readings were performed for each specimen using a spectrophotometer, and the evaluations were carried out in three different time. After the color reading, three averages and the standard deviation of variation were performed after 24 hours (T1), 7 days (T2) and 14 days (T3). Data were submitted to the ANOVA and 2 criteria and Tukey (P<0.05) in the statistical software SSPS 18 for Macintosh (SPSS Inc., Chicago, USA).Results: When compared the solutions in each group of material, there was no statistically significant difference, except for T3, where the group Dencor Brush and Bisacrylic demonstrated higher color variation in all the solutions, even in the control group, and the values in Chlorhexidine higher than the other, showing greater instability after 14 days.Conclusions: With the results, bisacrylic resin used as provisory prosthesis material presents greater color instability than the ARCA, when submitted to different solutions. Bisacrylic resin and Dencor Brush present significantly visible color changes in chlorhexidine solution after 14 days. All the materials in coke solution present homogeneity in the color change after 7 days exposition to the solution, with no visible color change.


2019 ◽  
Vol 10 (1) ◽  
pp. 14 ◽  
Author(s):  
Paulo Palma ◽  
Joana Marques ◽  
Rui Falacho ◽  
Eder Correia ◽  
Alexandra Vinagre ◽  
...  

Aim: The purpose of the present study is to assess the color stability of two calcium silicate-based cements (CSCs) used in regenerative endodontic procedures (REPs). Methods: A total of 40 acrylic single-rooted transparent teeth, with immature apex, were used. Root canals were filled up to 3 mm below the level of the cementoenamel junction, with either saline solution (Mineral Trioxide Aggregate (MTA)/saline and Biodentine/saline) or blood (MTA/blood and Biodentine/blood). Subsequently, ProRoot MTA® or BiodentineTM was placed in the root canal to create a cervical barrier. Color measurement was carried out at four different evaluation periods (3 h, 72 h, 7 days, and 6 months). Shade analysis within the L* a* b* color space was performed and color variation (∆E) calculated. The significance level for statistical analysis was set at p < 0.05. Results: The four groups showed a significant decrease in L* values over time. The ΔE value increased over time for all groups but was not statistically significant for the Biodentine/blood group. Two-way ANOVA showed no interaction between the CSC and treatment (contact with saline solution or blood). CSC used was the factor responsible for ΔE over time, inducing statistically significant color variations from T3H to T7D (p = 0.04) and T3H to T6M (p < 0.01). After 6 months, MTA/saline had 5.08 (p = 0.001) higher ΔE than Biodentine/Saline and the MTA/blood had 3.65 (p = 0.009) higher than Biodentine/blood. Conclusions: After 6 months, regardless of blood exposure, Biodentine exhibits superior color stability compared to MTA. Biodentine might be a suitable alternative to MTA as a cervical barrier material in REPs.


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