Label-free comparative proteomic and physiological analysis provides insight into leaf color variation of the golden-yellow leaf mutant of Lagerstroemia indica

2020 ◽  
Vol 228 ◽  
pp. 103942
Author(s):  
Sumei Li ◽  
Shuan Wang ◽  
Peng Wang ◽  
Lulu Gao ◽  
Rutong Yang ◽  
...  
Keyword(s):  
Color Variation ◽  
Yellow Leaf ◽  
Label Free ◽  
Leaf Color ◽  
Golden Yellow ◽  
Physiological Analysis ◽  
Leaf Mutant ◽  
Insight Into ◽  
Leaf Color Variation

scholarly journals Leaf Color Stability during Plant Development as an Index of Leaf Color Variation among Micropropagated Caladium

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 328-332 ◽  
Author(s):  
Eakhlas U. Ahmed ◽  
Takahiro Hayashi ◽  
Susumu Yazawa
Keyword(s):  
Plant Development ◽  
Leaf Explants ◽  
Stability Index ◽  
Color Stability ◽  
Color Variation ◽  
Leaf Color ◽  
Color Distribution ◽  
Dominant Color ◽  
Leaf Color Variation ◽  
Color Variants

The developmental pattern of leaf color distribution during plant development in 10 cultivars of Caladium ×hortulanum Birdsey was investigated. We used the color occupying the largest area in the terminal leaf as the dominant color, and expressed the leaf color stability during plant development by the ratio of the percentage of the dominant color area in the terminal leaf to that of the dominant color area in the initial leaf (leaf color stability index). In some cultivars, leaf color stability index was clearly greater than 1 (leaf-color-unstable cultivar), but in some cultivars it was close to 1 (leaf-color-stable cultivar). In plants regenerated from leaf explants of leaf-color-unstable cultivars, many (21% to 43%) color variants were observed but only a few (0% to 6%) occurred from leaf explants of leaf-color-stable cultivars. Tissue culture appears to be a useful technique for rapid propagation based on leaf color stability in leaf-color-stable and leaf-color-unstable cultivars.

Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indica yellow leaf mutant

2015 ◽  
Vol 37 (10) ◽  
pp. 851-863 ◽  
Author(s):  
Ya Li ◽  
Zhenyu Zhang ◽  
Peng Wang ◽  
Shu’an Wang ◽  
Lingling Ma ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Yellow Leaf ◽  
Leaf Color ◽  
Leaf Mutant

scholarly journals Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica

2015 ◽  
Vol 14 (4) ◽  
pp. 11827-11840 ◽  
Author(s):  
H.Y. Yang ◽  
X.W. Xia ◽  
W. Fang ◽  
Y. Fu ◽  
M.M. An ◽  
...  
Keyword(s):  
Color Variation ◽  
Leaf Color ◽  
Leaf Color Variation

scholarly journals Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicum L.), Siyl-1

2018 ◽  
Author(s):  
Tongmei Gao ◽  
Shuangling Wei ◽  
Jing Chen ◽  
Yin Wu ◽  
Feng Li ◽  
...  
Keyword(s):  
Morphological Structure ◽  
Nuclear Gene ◽  
Regulation Mechanism ◽  
Yellow Leaf ◽  
Leaf Color ◽  
Biochemical Effect ◽  
Effect Analysis ◽  
Light Yellow ◽  
Color Mutation ◽  
Leaf Mutant

AbstractLeaf color mutation in sesame always affects the growth and development of plantlets, and their yield. To clarify the mechanisms underlying leaf color regulation in sesame, we analyzed a yellow-green leaf mutant. Genetic analysis of the mutant selfing revealed 3 phenotypes—YY, light-yellow (lethal); Yy, yellow-green; and yy, normal green—controlled by an incompletely dominant nuclear gene, Siyl-1. In YY and Yy, the number and morphological structure of the chloroplast changed evidently, with disordered inner matter, and significantly decreased chlorophyll content. To explore the regulation mechanism of leaf color mutation, the proteins expressed among YY, Yy, and yy were analyzed. All 98 differentially expressed proteins (DEPs) were classified into 5 functional groups, in which photosynthesis and energy metabolism (82.7%) occupied a dominant position. Our findings provide the basis for further molecular mechanism and biochemical effect analysis of yellow leaf mutants in plants.

scholarly journals Physiological and transcriptomic analysis of yellow leaf coloration inPopulus deltoidesMarsh

2018 ◽  
Author(s):  
Shuzhen Zhang ◽  
Xiaolu Wu ◽  
Jie Cui ◽  
Fan Zhang ◽  
Xueqin Wan ◽  
...  
Keyword(s):  
Populus Deltoides ◽  
Color Variation ◽  
Deciduous Tree ◽  
Transcriptional Level ◽  
Yellow Leaf ◽  
Wild Type ◽  
Flavonoid Content ◽  
Leaf Color ◽  
Leaf Coloration ◽  
Chl Biosynthesis

AbstractAs important deciduous tree,Populus deltoidesMarsh possesses a high ornamental value for its leaves remaining yellow during the non-dormant period. However, little is known about the regulatory mechanism of leaf coloration inPopulus deltoidesMarsh. Thus, we analyzed physiological and transcriptional differences of yellow leaves (mutant) and green leaves (wild-type) ofPopulus deltoidesMarsh. Physiological experiments showed that the contents of chlorophyll (Chl) and carotenoid are lower in mutant, the flavonoid content is not differed significantly between mutant and wild-type. Transcriptomic sequencing was further used to identify 153 differentially expressed genes (DEGs). Functional classifications based on Gene Ontology enrichment and Genomes enrichment analysis indicated that the DEGs were involved in Chl biosynthesis and flavonoid biosynthesis pathway. Among these, geranylgeranyl diphosphate (CHLP) genes associated with Chl biosynthesis showed down-regulation, while chlorophyllase (CLH) genes associated with Chl degradation were up-regulated in yellow leaves. The expression levels of these genes were further confirmed using quantitative real-time PCR (RT-qPCR). Furthermore, the measurement of the main precursors of Chl confirmed that CHLP is vital enzymes for the yellow leaf color phenotype. Consequently, the formation of yellow leaf color is due to disruption of Chl synthesis and catabolism rather than flavonoid content. These results contribute to our understanding of mechanisms and regulation of leaf color variation in poplar at the transcriptional level.

scholarly journals Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mingyue Fu ◽  
Zhongcheng Zhou ◽  
Xu Yang ◽  
Zhongbing Liu ◽  
Jiarui Zheng ◽  
...  
Keyword(s):  
Carotenoid Biosynthesis ◽  
Color Variation ◽  
Carotenoid Content ◽  
Comparative Transcriptome ◽  
Leaf Color ◽  
Camellia Japonica ◽  
Leaf Phenotype ◽  
Color Mutation ◽  
Variation Mechanism ◽  
Leaf Color Variation

Abstract Background Leaf color variation is a common trait in plants and widely distributed in many plants. In this study, a leaf color mutation in Camellia japonica (cultivar named as Maguxianzi, M) was used as material, and the mechanism of leaf color variation was revealed by physiological, cytological, transcriptome and microbiome analyses. Results The yellowing C. japonica (M) exhibits lower pigment content than its parent (cultivar named as Huafurong, H), especially chlorophyll (Chl) and carotenoid, and leaves of M have weaker photosynthesis. Subsequently, the results of transmission electron microscopy(TEM) exhibited that M chloroplast was accompanied by broken thylakoid membrane, degraded thylakoid grana, and filled with many vesicles. Furthermore, comparative transcriptome sequencing identified 3,298 differentially expressed genes (DEGs). KEGG annotation analysis results showed that 69 significantly enriched DEGs were involved in Chl biosynthesis, carotenoid biosynthesis, photosynthesis, and plant-pathogen interaction. On this basis, we sequenced the microbial diversity of the H and M leaves. The sequencing results suggested that the abundance of Didymella in the M leaves was significantly higher than that in the H leaves, which meant that M leaves might be infected by Didymella. Conclusions Therefore, we speculated that Didymella infected M leaves while reduced Chl and carotenoid content by damaging chloroplast structures, and altered the intensity of photosynthesis, thereby causing the leaf yellowing phenomenon of C. japonica (M). This research will provide new insights into the leaf color variation mechanism and lay a theoretical foundation for plant breeding and molecular markers.

scholarly journals iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism of Golden-Yellow Leaf Mutant in Hybrid Paper Mulberry

2021 ◽  
Vol 23 (1) ◽  
pp. 127
Author(s):  
Fenfen Wang ◽  
Naizhi Chen ◽  
Shihua Shen
Keyword(s):  
Chlorophyll Synthesis ◽  
Carotenoid Content ◽  
Yellow Leaf ◽  
Mesophyll Cells ◽  
Chlorophyll Metabolism ◽  
Growth And Survival ◽  
Leaf Phenotype ◽  
Golden Yellow ◽  
Leaf Mutant ◽  
The Impact

Plant growth and development relies on the conversion of light energy into chemical energy, which takes place in the leaves. Chlorophyll mutant variations are important for studying certain physiological processes, including chlorophyll metabolism, chloroplast biogenesis, and photosynthesis. To uncover the mechanisms of the golden-yellow phenotype of the hybrid paper mulberry plant, this study used physiological, cytological, and iTRAQ-based proteomic analyses to compare the green and golden-yellow leaves of hybrid paper mulberry. Physiological results showed that the mutants of hybrid paper mulberry showed golden-yellow leaves, reduced chlorophyll, and carotenoid content, and increased flavonoid content compared with wild-type plants. Cytological observations revealed defective chloroplasts in the mesophyll cells of the mutants. Results demonstrated that 4766 proteins were identified from the hybrid paper mulberry leaves, of which 168 proteins displayed differential accumulations between the green and mutant leaves. The differentially accumulated proteins were primarily involved in chlorophyll synthesis, carotenoid metabolism, and photosynthesis. In addition, differentially accumulated proteins are associated with ribosome pathways and could enable plants to adapt to environmental conditions by regulating the proteome to reduce the impact of chlorophyll reduction on growth and survival. Altogether, this study provides a better understanding of the formation mechanism of the golden-yellow leaf phenotype by combining proteomic approaches.

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