scholarly journals Effects of Cooking and Storage on Antioxidant Activity in Potato (Solanum tuberosum L.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 879D-879
Author(s):  
Tyann Blessington* ◽  
Anna L. Hale ◽  
Douglas C. Scheuring ◽  
J. Creighton Miller

We have demonstrated that potatoes contain significant levels of antioxidants important to human health; however, since potatoes are not consumed raw, it is important to determine the effects of cooking/processing on these levels. Therefore, the changes in phenolic and carotenoid content and total antioxidant activity in potatoes were investigated using combinations of storage and cooking methods. Fresh and stored tubers (110 days at 4 °C) of 17 potato cultivars, both raw and cooked (microwaved, boiled, baked, fried), were analyzed for antioxidant activity using the DPPH method. In addition, carotenoid levels were determined for each treatment based on the absorbance of the methanol extraction (oxygenated phenolics and carotenoids) at 445 nm and the hexane extraction (non-oxygenated carotenoids) at 450 nm. Total antioxidant activity as well as carotenoid levels were significantly affected by both genotype and cooking method. Across extraction methods, the microwave and fry cooking treatments were generally highest in antioxidant activity, while boiling was the lowest. Oxygenated carotenoids were significantly affected by storage, while the non-oxygenated carotenoids were unaffected.

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 869D-869
Author(s):  
Peter J. Mes* ◽  
James R. Myers

Tomato lines carrying the genes Aft, atv, Abg, hp-1, and an as yet undetermined gene from the introgression line LA2099 have been combined to produce fruit with elevated anthocyanin content. The antioxidant activity of juice made from anthocyanin-expressing tomatoes was compared to juices made from tomatoes with varied carotenoid content. The contribution of anthocyanin to the total antioxidant activity of the whole fruit in current material is small, but with potential for significant improvement. The increase in flavonoids in the elevated anthocyanin lines has increased water-soluble antioxidant activity of the fruit in vitro.


Author(s):  
Lucilene Dornelles Mello ◽  
Tiele Garcia Tunes

Objective: This study estimated the Permangante Index (IKMnO4) and evaluated its correlation with parameters as total phenols (FT) and total antioxidant activity (TAA) of commercial teas of Camellia sinensis, fermented, unfermented and blended with other plants.Methods: The aqueous extracts were prepared from commercial samples of green and black teas, green tea blended with lemon (Camellia sinensis with Citrus limonium), green tea blended with mint (Camellia sinensis with Mentha piperita), green tea blended with peach (Camellia sinensis with Prunus persica) and green tea blended with orange (Camellia sinensis with Citrus sinensis). The Permangante index was determined by titrating the tea extract with potassium permanganate solution according to AOAC method (AOAC, 1980) with modifications. The concentration of phenols in the extracts was determined by spectrophotometry according to the standard procedure of Folin-Ciocalteau. The antioxidant activity of the extracts was evaluated using the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH—) method. All the determinations were performed in triplicate, and the results presented as mean ± standard deviation (SD). The data were evaluated by univariate statistical analysis (ANOVA), F-test and mean test (Tukey´s test) for comparative results. Pearson’s correlation was used to measure the degree of linear correlation and the quantitative variables were from a normal population.Results: The results for Permanganate Index show a variation ranged from 0.40 to 0.80 mEq/l. The ANOVA analysis to IKMnO4 shown there is significant statistically difference at the 5% probability level (p < 0.05) between the types of tea as well as the amount of herb used to prepare the infusion. Therefore, it was observed that there significant contrast between tea extracts, the mixture of different plants in blended extracts as well as the tea manufacture process affect the polyphenols tannins content influencing the permanganate index parameter. Studies correlation shown strong correlations (r > 0.7) among the parameters. For the Camellia sinensis extracts the high correlations between IKMnO4 and TAA; FT and TAA demonstrates the suitability of the permanganate index in the evaluation of antioxidant activity.Conclusion: The results suggest that the Permanganate Index could be used for quality control of tea as an additional parameter.


2015 ◽  
Vol 7 (1) ◽  
pp. 242-248
Author(s):  
Sukhpreet Kaur ◽  
Poonam Aggarwal

The present study was carried out to develop papads (Indian cookie) from potato (Solanum tuberosum L.) and rice (Oryza sativa) blends and to analyze them for organoleptic, physicochemical, phytochemical and shelf life quality. Two processing cultivars (Kufri Chipsona-1, Kufri Chandramukhi) and one commonly grown cultivar (Kufri Pukhraj) were evaluated for processing into papad. Based on preliminary sensory trails, papads with boiled potato mash (60%) and gelatinized rice (30%) level of supplementation were found to be most acceptable and these papads were subjected to nutritional evaluation. Results were compared with rice papads (control). Protein content and yield was significantly (p<0.05) higher in control papads compared to potato supplemented papads. Oil uptake significantly (p<0.05) increased on supplementation with potato. Bioactive compounds including ascorbic acid, totalphenolics and total antioxidant activity measured as DPPH radical scavenging activity increased significantly (p<0.05) on incorporation of potato. Between the cultivars, papads enriched with Kufri Pukhraj, a table variety which is considered unfit for processing, displayed the highest phytochemical content and total antioxidant activity. Sensory evaluation indicated higher overall acceptability scores of potato enriched papads compared to control. During storage of papads at room temperature for up to 3 months, significant (p<0.05) changes in the moisture content, phytochemical content and antioxidant activity were observed. Storage studies showed that the potato supplemented papads can be stored safely for 3 months of storage at ambient temperature.


2010 ◽  
Vol 5 (3) ◽  
pp. 205 ◽  
Author(s):  
Evy Damayanthi ◽  
Lilik Kustiyah ◽  
Mahani Khalid ◽  
Henry Farizal

<p class="MsoNormal" style="margin: 0cm 5.65pt 6pt 14.2pt; text-align: justify; text-indent: 1cm;"><span style="font-size: 10pt;" lang="en-us" xml:lang="en-us">Recently, many foods were identified contain high antioxidant substances such as tomatoes and rice bran. The </span><span style="font-size: 10pt;">objectives</span><span style="font-size: 10pt;" lang="en-us" xml:lang="en-us"> of this research </span><span style="font-size: 10pt;">were</span><span style="font-size: 10pt;" lang="en-us" xml:lang="en-us"> to observe their total antioxidant activity values and the effect of intervention of high-antioxidant beverages (tomato juice and rice bran beverage) to total antioxidant activity on breast cyst women patient’s blood serum. Subject consumed each of the beverages for 14 days. Blood samples were taken before the intervention, at the end of tomato juice intervention period and at the end of rice bran bevera</span><span style="font-size: 10pt;">g</span><span style="font-size: 10pt;" lang="en-us" xml:lang="en-us">e intervention period. Total antioxidant activity on serum was determined by DPPH method. The ascorbic acid equivalent antioxidant capacity (AEAC) of rice bran greater than tomato juice is 28.74 mg/100g and 1.87 mg/100g respectively. The total antioxidant activity on serum was decreasing after tomato juice intervention (p&gt;0.05) but the decreasing of it was significantly shown after rice bran intervention (p&lt;0.05)</span><span style="font-size: 10pt;">.</span></p>


2021 ◽  
pp. 38-49
Author(s):  
Amos Asiimwe ◽  
Julia B. Kigozi ◽  
John Muyonga

Aims: The study evaluated the use of refractance window dried passion fruit powder as a flavoring for yogurt. Place and Duration of Study: Department of Food Technology and Nutrition, Makerere University, Kampala-Uganda between October 2020 and January 2021. Methodology: Passion fruit pulp was mixed in carboxymethyl cellulose and dried using a laboratory refractance window drier. Plain yogurt was flavored with 2% passion fruit powder. Passion fruit flavored, plain and commercial yogurt were analyzed for physico-chemical and sensory properties. Statistical analyses were performed using XLSTAT and all results were considered to be significant at P<0.05. Results: pH values and titratable acidity for all yogurts ranged from 4.67 to 3.77 and 1.21% to 2.89%, respectively throughout the 14 day storage period. The apparent viscosity of all yogurts increased significantly during storage and ranged from 413.76 cP to 525.20 cP, the syneresis of yogurts ranged from 25.6% to 32.2%, vitamin C content of the yogurts ranged from 0.69 mg/100 g to 1.91 mg/100 g, the carotenoid content of yogurts ranged from 0.55 µgRAE to 1.07 µgRAE, phenolic content of the yogurts ranged from 0.50 mg/100 g GAE to 8.01 mg/100 g GAE, whereas total antioxidant activity of the yogurts ranged from 0.21mg/100g VCE to 9.96mg/100g VCE. For all yogurt types, there was no significant change (p<0.05) in any of the sensory parameters (flavor, color, mouth feel, consistency, taste and overall acceptability) over the storage period of 14 days. Conclusion: Addition of passion fruit powder to yogurt resulted in increased levels of vitamin C, beta carotenoid, total phenolic content and total antioxidant activity, in comparison to plain yoghurt and commercial vanilla flavored yogurt. This will provide consumers with more nutritious options thereby improving nutrition of consumers.


2016 ◽  
Vol 24 (2) ◽  
pp. 290-294 ◽  
Author(s):  
A. K. Velygodska ◽  
O. V. Fedotov

The aim of the study was selection of optimal conditions for obtaining carotenoid drugs of mycelium origin from the basidiomycete strains Laetiporus sulphureus Ls-08, Fomes fomentarius Ff-1201 and Fistulina hepatica Fh-18 and the study of antibacterial and total antioxidant activity of these compounds. The strains were surface grown on a glucose-peptone medium modified for each producer. The homogenized pigments of the mycelium strains were extracted with ethanol and the solvent was separated under vacuum at 60 ºC. The absorption spectra of the carotenoid drugs were recorded for alcoholic solutions at 350–500 nm. The antibacterial activity of the carotenoids was determined by the agar diffusion method, and the total antioxidant activity was determined by the DPPH-method. It was found that the optimum temperature for carotenoid extraction is 60 °C. The absorption spectra of carotenoid drugs showed three peaks in 420, 450 and 470 nm. These results respond to the β-carotene absorption spectra. The highest antioxidant activity was noted for carotenoid drugs from F. hepatica Fh-18 and L. sulphureus Ls-08 strains obtained at an extraction temperature of 40 and 60 °C respectively. The antibacterial activity of carotenoid drugs against the test cultures was not species dependent. Carotenoid drugs with a 20% concentrate obtained from the L. sulphureus Ls-08 strain had the highest antibacterial activity against the test cultures Staphylococcus aureus and Escherichia coli. Carotenoids from the mycelium of F. hepatica Fh-18 had the highest antibacterial activity against the test culture Candida albicans. Extraction temperature of 60 °C is optimal for mycelial yield of carotenoids from the studied strains. All preparations of carotenoids exhibited antibacterial activity against the test microorganism cultures. The carotenoid drugs obtained at 40 and 60 °C from the strains F. hepatica Fh-18 and L. sulphureus Ls-08 respectively showed the highest antioxidant activity. 


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 876b-876
Author(s):  
Tyann Blessington ◽  
Douglas C. Scheuring ◽  
J. Creighton Miller

Potatoes are stored to ensure a continuous supply; however, losses due to shrinkage and sprouting can be large. It is believed that ionizing irradiation will become more prominent for sprout inhibition due to the increasingly higher operating costs of low-temperature storage and possible phase-out of chemical sprout inhibitors. The effects of storage and ionizing irradiation (gamma and electron beam) on antioxidant activity (AOA), phenolic content, and carotenoid content were analyzed using the potato cultivar Atlantic. Tubers were subjected to 0, 75, and 200 Gy γ-irradiation doses, stored at 20 °C, and analyzed after 0, 10, 20, 75, and 110 days. Tubers from another harvest were subjected to a surface dose of 0 or 200 Gy e-beam irradiation, stored at 20 °C, and analyzed after 0, 10, 20, 75, and 110 days. AOA was measured via the DPPH method; phenolic content via the Folin-Ciocalteau method and individual phenolics via HPLC; and carotenoid content via absorbance at 445 nm and individual carotenoids via HPLC. During early storage, higher doses resulted in higher AOA, while, during longer storage, lower doses produced greater AOA. Phenolic content increased in storage during the γ-irradiation study, but decreased in the e-beam study, partly due to increases in chlorogenic acid in the former and decreases in caffeic acid in the latter. The e-beam dose of 200 Gy resulted in significantly greater total phenolics than 0 Gy. Total carotenoids and lutein decreased with storage, but were not affected by irradiation. Storage exerted a much greater influence on AOA, phenolic content, and carotenoid content than either irradiation treatment.


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