scholarly journals (302) Photoautotrophic Micropropagation Systems Demonstrate Rooting Stage Improvement of Laelia purpurata var. alba

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1010D-1010
Author(s):  
Hope Jones ◽  
Chieri Kubota
Keyword(s):  
Root Growth ◽  
Survival Rates ◽  
Photon Flux ◽  
Ex Vitro ◽  
Photosynthetic Photon Flux ◽  
In Vitro Systems ◽  
Vigorous Growth ◽  
Acclimatization Period ◽  
Free Media

In vitro culture of orchid plantlets within conventional photomixotrophic micropropagation (PMM) systems (sucrose containing media in a non-enriched CO2 environment) often induces vigorous growth and multiplication. However, transition to ex vitro conditions frequently results in significant plantlet loss during the acclimatization process. Recent studies investigating micropropagation within photoautotrophic (PAM) systems (sucrose-free media in enriched CO2 conditions) have demonstrated improved plantlet survival during the acclimatization period due to greater root growth and stomata adaptation. Laelia purpurata var. alba, an orchid with many endangered relatives, was chosen as a model orchid species to investigate if plantlet culture within PAM in vitro systems has the potential to improve propagation success and ex vitro survival of endangered Laelia species. Protocorm-like bodies with developed two fully extended leaves were transferred into PMM (photosynthetic photon flux 50 μmol·m-2·s-1 under non-enriched CO2 conditions) and PAM (photosynthetic photon flux 150 μmol·m-2·s-1, CO2 level enriched to 1500 μmol·mol-1) systems. After 6 weeks, plantlet rooting within the PMM system was variable and inconsistent, while all PAM plantlets produced healthy robust root systems. Average fresh weights and percent shoot development were not significantly different between treatments. Induction of improved root growth by PAM systems may improve orchid plantlet survival rates during acclimatization and advance our ability to increase endangered orchid populations.

scholarly journals Effects of LED light on Acacia melanoxylon bud proliferation in vitro and root growth ex vitro

2019 ◽  
Vol 14 (1) ◽  
pp. 349-357 ◽  
Author(s):  
Shubin Li ◽  
Lili Zhou ◽  
Sipan Wu ◽  
Li Liu ◽  
Meng Huang ◽  
...  
Keyword(s):  
Light Intensity ◽  
Root Growth ◽  
Photon Flux ◽  
Light Illumination ◽  
Root Number ◽  
Ex Vitro ◽  
Blue Led ◽  
Positive Effects ◽  
Acacia Melanoxylon

AbstractThis study examines the effects of light emitting diodes (LEDs) on tissue culture proliferation of Acacia melanoxylon plantlets among five different clones (FM1, FM2, FM4, FM5, and FM10). Shoot bud apex cuttings were transplanted onto Murashige and Skoog basal medium containing 0.1 mg L-1 6-benzyladenine and 0.5 mg L-1 naphthalene acetic acid and cultured in vitro for 40 days. Root growth was studied under different light intensities and photoperiods ex vitro. The bud proliferation coefficient was greatest under a light intensity of 45 μmol m-2 s-1 photosynthetic photon flux and photoperiod of 16 h light, but decreased as the light intensity increased. However, the greatest light intensity was beneficial for the growth of robust plantlets. Plantlets exposed to red and blue LED combinations grew tall and green, with a small number of roots. Plantlets also grew taller and some roots expanded under the longer photoperiod. Increased light intensity had positive effects on root number and rooting rate, and prolonged light greatly increased root number. Therefore, lower light intensity and a short photoperiod were beneficial for bud proliferation, while red/blue LED combinations, increased light intensity, and longer light illumination were beneficial for plantlet growth and root growth of Acacia melanoxylon.

scholarly journals Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

2021 ◽  
Vol 22 (1) ◽  
pp. 17-30
Author(s):  
Nataliya Dimitrova ◽  
Lilyana Nacheva ◽  
Małgorzata Berova ◽  
Danuta Kulpa
Keyword(s):  
Woody Species ◽  
Photosynthetic Photon Flux Density ◽  
Stem Length ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Ex Vitro ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Number Of Leaves

In vitro micropropagation of plants is highly useful for obtaining large quantities of planting material with valuable economic qualities. However, plantlets grow in vitro in a specific environment and the adaptation after the transfer to ex vitro conditions is difficult. Therefore, the acclimatization is a key step, which mostly determines the success of micropropagation. The aim of this investigation was to study the effect of the biofertlizer Lumbrical on ex vitro acclimatization of micropropagated pear rootstock OHF 333 (Pyrus communis L.). Micropropagated and rooted plantlets were potted in peat and perlite (2:1) mixture with or without Lumbrical. They were grown in a growth chamber at a temperature of 22±2 °C and photoperiod of 16/8 hours supplied by cool-white fluorescent lamps (150 µmol m-2 s-1 Photosynthetic Photon Flux Density, PPFD). The plants were covered with transparent foil to maintain the high humidity, and ten days later, the humidity was gradually decreased. Biometric parameters, anatomic-morphological analyses, net photosynthetic rate and chlorophyll a fluorescence (JIP test) were measured 21 days after transplanting the plants to ex vitro conditions. The obtained results showed that the plants, acclimatized ex vitro in the substrate with Lumbrical, presented better growth (stem length, number of leaves, leaf area and fresh mass) and photosynthetic characteristics as compared to the control plants. This biostimulator could also be used to improve acclimatization in other woody species

CO2-Enrichment and photosynthetic photon flux affect the growth of in vitro-cultured apple plantlets

2001 ◽  
Vol 44 (2) ◽  
pp. 87-91 ◽  
Author(s):  
Ru Yu Li ◽  
Hosakatte Niranjana Murthy ◽  
Seon Kyu Kim ◽  
Kee Yeoup Paek
Keyword(s):  
Co2 Enrichment ◽  
Photon Flux ◽  
Photosynthetic Photon Flux

scholarly journals EFFECT OF LIGHT, CO2, AND SUCROSE CONCENTRATIONS ON THE IN VITRO GROWTH OF WHITE POTATO (SOLANUM TUBEROSUM L.)

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696f-696
Author(s):  
N.C. Yorio ◽  
R.M. Wheeler ◽  
R.C. Weigel
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Dry Weight ◽  
Growth Chamber ◽  
Photon Flux ◽  
Photosynthetic Photon Flux ◽  
White Potato ◽  
High Sucrose ◽  
Effect Of Light

Growth measurements of potato (Solanum tuberosum L.) cvs. Norland (NL), Denali (DN), and Kennebec (KN) were taken from 21-day-old plantlets grown in vitro. Studies were conducted in a growth chamber, with nodal explants grown in culture tubes with loose-fitted Magenta 2-way caps containing Murashige and Skoog salts with either 0, 1, 2 or 3% sucrose. The cultures received either 100 or 300 μmol m-2 s-1 photosynthetic photon flux (PPF), and the growth chamber was maintained at either 400 or 4000 μmol mol-1 CO2. All cvs. showed significant increases in growth on 0% sucrose media at 4000 μmol mol-1 CO2, indicating an autotrophic response. At 400 μmol mol-1 CO2, all cvs. showed an increase in total plantlet dry weight (DW) with increasing sucrose under both PPF levels. Within any sucrose treatment, the highest total DW for all cvs. resulted from 300 μmol m-2 s-1 PPF and 4000 μmol mol-1 CO2. At 4000 μmol mol-1 CO2, shoot DW declined with sucrose above 2% for DN and sucrose above 1% for NL at both PPF levels, suggesting that high sucrose levels may hinder growth when CO2 enrichment is used.

scholarly journals Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

Plants ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 3 ◽  
Author(s):  
Vadim Lebedev ◽  
Mikhail Arkaev ◽  
Mariya Dremova ◽  
Ivan Pozdniakov ◽  
Konstantin Shestibratov
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Survival Rates ◽  
Ex Vitro Rooting ◽  
Rubus Idaeus ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Gelling Agents ◽  
The Cost

Successful acclimatization and ex vitro rooting are among the key factors reducing the cost of micropropagated plants. We compared the survival of seven Russian cultivars of raspberry (Rubus idaeus) after rooting in vitro and ex vitro. Rooted shoots adapted to nonsterile conditions much better than nonrooted ones, with survival rates of 81%–98% versus 43%–76%, respectively. We studied the effects of different combinations of plant-growth regulators and gelling agents added to a proliferation medium on ex vitro rooting of primocane-fruiting raspberry cultivar “Atlant”. Reducing the agar concentration from 8 to 6.5 g/L increased the multiplication rate, but caused shoot hyperhydricity. The highest survival rate (97.2%) was observed for shoots grown in a medium containing 0.2 and 0.1 mg/L IBA, and gelled with 5 g/L agar and 0.2 g/L Phytagel. The microshoot height at the multiplication stage did not correlate with the plant growth during acclimatization. The obtained results can be used in the commercial micropropagation of the raspberry.

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