scholarly journals Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry

Plants ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 3 ◽  
Author(s):  
Vadim Lebedev ◽  
Mikhail Arkaev ◽  
Mariya Dremova ◽  
Ivan Pozdniakov ◽  
Konstantin Shestibratov
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Survival Rates ◽  
Ex Vitro Rooting ◽  
Rubus Idaeus ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Gelling Agents ◽  
The Cost

Successful acclimatization and ex vitro rooting are among the key factors reducing the cost of micropropagated plants. We compared the survival of seven Russian cultivars of raspberry (Rubus idaeus) after rooting in vitro and ex vitro. Rooted shoots adapted to nonsterile conditions much better than nonrooted ones, with survival rates of 81%–98% versus 43%–76%, respectively. We studied the effects of different combinations of plant-growth regulators and gelling agents added to a proliferation medium on ex vitro rooting of primocane-fruiting raspberry cultivar “Atlant”. Reducing the agar concentration from 8 to 6.5 g/L increased the multiplication rate, but caused shoot hyperhydricity. The highest survival rate (97.2%) was observed for shoots grown in a medium containing 0.2 and 0.1 mg/L IBA, and gelled with 5 g/L agar and 0.2 g/L Phytagel. The microshoot height at the multiplication stage did not correlate with the plant growth during acclimatization. The obtained results can be used in the commercial micropropagation of the raspberry.

scholarly journals In vitro propagation of bastard balm (Melittis melissophyllum L.)

2015 ◽  
Vol 61 (3) ◽  
pp. 67-76
Author(s):  
Katarzyna Bączek ◽  
Izabela Szymborska-Sandhu ◽  
Anna Pawełczak ◽  
Zenon Węglarz
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Morphological Traits ◽  
In Vitro Propagation ◽  
Axillary Shoot ◽  
Shoot Branching ◽  
Ex Vitro ◽  
Root Regeneration ◽  
Auxin Concentration

Summary An efficient method for in vitro propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish in vitro culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0 mg/l BA with 0.01 mg/l NAA or without the auxin. The highest number of shoots per explant was obtained on the medium with 1.0 mg/l BA (3.9 shoots per explant). For the rooting of shoots ½ MS/B5 with IBA (0.25, 0.50 and 1.0 mg/l) medium was used. The medium without plant growth regulators served as a control. The best root regeneration was observed on the medium without IBA (87.1% of cuttings rooted). IBA used in the medium for shoot rooting affected the morphological traits of obtained microcuttings but not affected their weight. Irrespective of auxin concentration in this medium, obtained microcuttings acclimated in ex vitro conditions very well.

scholarly journals Optimising Gelling Agents, Light Source and After-care to Commercialise Teak Tissue Culture

2018 ◽  
Vol 28 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Ramasamy Mahalakshmi ◽  
Manikantan Vijayamma Vineetha ◽  
Rekha Ravindranath Warrier
Keyword(s):  
Shoot Multiplication ◽  
Tectona Grandis ◽  
Multiple Shoot ◽  
Steady Increase ◽  
Light Sources ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Gelling Agents ◽  
Significant Difference

Mass propagation of Teak (Tectona grandis Linn. f.) a commercial species is a priority to increase multiplication rate and meet the growing demand for planting material. The present study has assessed the in vitro performance of Teak Tissue Cultures with different gelling agents, different light conditions, and aftercare of ex vitro rooted shoots to enhance their survival rate. Multiple shoot formation was induced from excised seedling nodal explants on MS supplemented with BA and Kn and about 5 - 10 shoots were obtained from each explant. Significant variation (p > 0.05) was observed in the number of shoots produced by the different clones. Three gelling agents (agar, phytagel and gellan gum), and two light sources (tube light and LED) were tested for enhancing shoot multiplication. No significant difference in in vitro growth was observed between clones with different solidifying agents. Teak, however, did not respond favourably to LED lights. Rooting-acclimatization phase was achieved in the nursery with 80 - 95 per cent success. The rooted plants were sprayed with DAP and Humaur to assess the growth performance following transplanting. Significant variations in rooting indicate the existence of physiological variations among the clones. Application of fertilizers promoted an initial boost followed by a steady increase during the rest of the study period. Clones with high multiplication rates under in vitro conditions could be selected for commercialization of teak multiplication. Under ex vitro conditions, a spray of fertilizers during the initial establishment phase would result in increased vigour of transplantable plants. This would ensure better survival on out planting.Plant Tissue Cult. & Biotech. 28(1): 13-24, 2018 (June)

scholarly journals The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens

2008 ◽  
Vol 20 (2) ◽  
pp. 93-102 ◽  
Author(s):  
Teresa Cybularz-Urban ◽  
Ewa Hanus-Fajerska
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Plant Material ◽  
Viral Infections ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Viral Pathogens ◽  
Morphogenesis In Vitro ◽  
Different Origin

Abstract The micropropagation efficiency of four interspecific Cattleya hybrids (clones: 69, 75, 149 and 150) infected with Cymbidium mosaic (CyMV) and Odontoglossum ringspot (ORSV) viruses was assessed. The aim of experiments was to evaluate with that model to what extent viral infection affects the morphogenesis in vitro in orchid hybrids of different origin. The effectiveness of plant material exposure to therapeutic levels of plant growth regulators supplied with media in order to suppress infection was also verified. The vitality of proliferating infected shoot cultures was limited, and the symptoms of senility were frequently observed. Regardless genotype of the studied clone, during acclimation to ex vitro conditions considerable losses become visible what indicates the necessity of testing the donor material for possible latent viral infections. Infection with CyMV and ORSV mostly persisted in every tested clone.

scholarly journals The elaboration of a practical protocol for the micropropagation of several apple rootstock varieties

2016 ◽  
Vol 73 (2) ◽  
pp. 226
Author(s):  
Doina Clapa ◽  
Alexandru Fira ◽  
Manuela Simu ◽  
Monica Harta ◽  
Cristian Sisea
Keyword(s):  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Wheat Starch ◽  
Axillary Shoot ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Apple Rootstock ◽  
Axillary Shoot Proliferation ◽  
D 20

The apple rootstock varieties ‘MM 106/4’, ‘MM 106/6’, ‘D 18’, ‘D 20’, ‘JTE-H’ and ‘MR 09/4’ were multiplied in vitro on modified Murashige and Skoog media gelled with wheat starch (MSs) and supplemented with 0.7 mg/l BA, which provided intense axillary shoot proliferation. Among the genotypes we studied, MR 09/4 had the highest multiplication rate  (19.56), followed by D18 (15.36). The lowest multiplication rates were recorded in MM 106/6 (5.36) and in MM 106/4 (3.32). The use of the technique of direct ex vitro rooting and acclimatization in floating perlite provided rooting percentages of more than 90 %.

scholarly journals ENRAIZAMENTO EX VITRO E ACLIMATIZAÇÃO DE PLANTAS MICROPROPAGADAS DE Tectona grandis

FLORESTA ◽  
2011 ◽  
Vol 41 (1) ◽  
Author(s):  
Paulo César Poeta Fermino Júnior ◽  
Andrea Raposo ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Tree Species ◽  
Tectona Grandis ◽  
Ex Vitro Rooting ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Relative Growth ◽  
Juvenile Plants ◽  
Plastic Trays

O enraizamento de espécies arbóreas é bastante complexo devido à maturidade dos tecidos. O objetivo deste trabalho foi avaliar o efeito de AIB no enraizamento ex vitro de brotos micropropagados a partir de plantas jovens de Tectona grandis L. em dois substratos, bem como da porção do broto para enraizamento. Foram utilizados brotos multiplicados in vitro inteiros, ou excisados ao meio (porção apical e basal), submetidos a imersão por 10 segundos em soluções contendo diferentes concentrações de AIB (0, 100, 1000, 2000, 4000 mg.L-1) e plantados em bandejas plásticas contendo vermiculita ou Plantmax® como substratos. O enraizamento ex vitro ocorreu em todos os tratamentos, inclusive na ausência de AIB. O maior número de raízes e o maior crescimento relativo do caule foram observados para os tratamentos com o uso de AIB, em ambos os substratos. O enraizamento ocorreu em 100% dos explantes de origem apical e basal, em ambos os substratos. O enraizamento ex vitro de brotos micropropagados de T. grandis é viável a partir de plantas jovens, e a taxa de multiplicação é duplicada com o seccionamento dos brotos em porções apical e basal.Palavras-chave:  Teca; produção de mudas; micropropagação; condição ex vitro; porção do broto. AbstractEx vitro rooting and acclimatization of micropropagated plantlets of Tectona grandis. The rooting of tree species is very complex due to the maturation of tissues. The objective of this study was to evaluate the effect of IBA on ex vitro rooting of micropropagated shoots from juvenile plants of Tectona grandis L. into two substrates, as well as the portion of the shoot to root. Shoots multiplied in vitro were used with whole or excised shoots (apical and basal) submitted to immersion for 10 seconds in solutions of IBA (0, 100, 1000, 2000, 4000 mg.L-1) and planted in plastic trays containing vermiculite or Plantmax ® as substrates. The ex vitro rooting occurred in all treatments, even in the absence of IBA. The largest number of roots and higher relative growth of the stem were observed in all treatments with IBA in both substrates. The survival of acclimatized plantlets occurred in all treatments. Rooting occurred in 100% of explants from apical and basal origin in both substrates. The ex vitro rooting of micropropagated shoots of T. grandis is feasible from juvenile plants and the multiplication rate is duplicated when shoots are cutin apical and basal portions.Keywords: Teak; seedlings; micropropagation; ex vitro condition; portion of the shoot.

scholarly journals IMPACT OF GROWTH REGULATORS ON IN VITRO GROWTH OF BANANA (MUSA SPP) CULTURED: A REVIEW

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Katkam Priyanka
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Musa Spp ◽  
Micro Propagation

Banana is important fruit crop in horticulture where it propagates through suckers which takes immense time for production and with low multiplication rate, to overcome these type of situation some protocols have been made such as the micro propagation where the tissue culture of banana is applied with help of plant growth regulators such as auxins and cytokinins are used at different concentration to attain the definite good results till now many studies had been done in the tissue culture of banana. In the present study it was observed that the explants cultured in MS medium containing 4 mg/l BAP + 0.5 mg/l IAA had highest number of shoot buds and number of shoots. Similar result was studied by Muhammad et al. (2007) where the highest multiplication ratio was observed at 4 mg/l BAP along with 1 mg/l IAA. Habiba et al. (2002) and Ahmed et al. (2014) reported that 4 mg/l BAP in combination with 2 mg/l IAA shown remarkable results.

scholarly journals An Optimized Micropropagation Protocol by Ex Vitro Rooting of Pear Rootstock OHF 333 (Pyrus communis L.)

2021 ◽  
Vol 74 ◽  
Author(s):  
Nataliya Dimitrova ◽  
Lilyana Nacheva
Keyword(s):  
Survival Rate ◽  
Plant Growth ◽  
Root Length ◽  
Pyrus Communis ◽  
Ex Vitro Rooting ◽  
Naphthaleneacetic Acid ◽  
Moderate Degree ◽  
Ex Vitro ◽  
Number Of Leaves

Abstract Pear rootstock OHF 333 ( Pyrus communis L.), which is included in the US ‘Old Home’ × ‘Farmingdale’ series, is characterized by good compatibility with most other varieties, high yields, and a moderate degree of resistance to fire blight. Micropropagation in vitro has shown promise for rapid, large-scale cloning of disease-free plant material throughout the year. However, pear shoots are often recalcitrant to rooting, and this process is highly genotype-dependent. This study aimed to improve the micropropagation protocol by ex vitro rooting of pear rootstock OHF 333 ( Pyrus communis L. ‘Old Home’ × ‘Farmingdale’). Charkor, a new plant growth regulator of natural origin was used, which contains metabolism products of symbiotic fungus-endophytes of ginseng roots, as an alternative to synthetically produced plant growth regulators (PGRs). Microcuttings were obtained from in vitro cultured shoots and subjected to four different treatments for ex vitro rooting: 1 g L −1 1-naphthaleneacetic acid (NAA) (as a powder), 0.5 mL L −1 Charkor for 3 hr or 6 hr, or the same concentration of Charkor prepared as a powder. Microshoots dipped in sterile distilled water with no additional hormonal treatments served as controls. Cultures were kept in a growth chamber under a 16-hr photoperiod, with air humidity maintained close to 100% (above 96%) for 2 weeks and then gradually reduced to 60%. Data on final acclimatization rate (survival rate; %), mean number of roots per plant, stem and root length, mean number of leaves per plant, and final acclimatization rate were collected 90 days after transplanting to ex vitro conditions. All treatments induced a successful acclimatization rate of more than 31%. The highest survival rate (86%) and longest stems were achieved by treatment with 0.5 mL L −1 Charkor for 6 hr. The greatest mean number of roots per plant, root length, and number of leaves was achieved in the variant treated with 1 g L −1 powdered NAA.

scholarly journals An Efficient Ex Vitro Rooting and Acclimatization Method for Horticultural Plants Using Float Hydroculture

HortScience ◽  
2013 ◽  
Vol 48 (9) ◽  
pp. 1159-1167 ◽  
Author(s):  
Doina Clapa ◽  
Alexandru Fira ◽  
Nirmal Joshee
Keyword(s):  
Vaccinium Corymbosum ◽  
Rosa Hybrida ◽  
Ex Vitro Rooting ◽  
Rubus Idaeus ◽  
Ex Vitro ◽  
Lycium Barbarum ◽  
Rubus Fruticosus ◽  
Floating Cell ◽  
Drosera Rotundifolia

This article presents a new acclimatization method, ex vitro acclimatization in float hydroculture. This protocol ensures the ex vitro acclimatization of the plantlets obtained in vitro in the rooting stage or the ex vitro rooting and acclimatization of the shoots obtained in the multiplication stage. Our hydroculture method is different from the techniques of flotation hydroculture because no fertilizers or plant growth regulators or other biostimulators are used and water oxygenation by bubbling is not provided. Ex vitro rooting and acclimatization in one stage without an in vitro rooting stage in floating cell trays was successfully carried out in Rubus fruticosus and Rosa hybrida cultivars, whereas ex vitro acclimatization of in vitro-rooted plantlets was successfully carried out in Rubus idaeus, Ribes nigrum, Prunus cerasus × P. canescens, Lycium barbarum (Goji berry), Amelanchier canadensis, Drosera rotundifolia, Drosera capillaris, and Nephrolepis sp. In another set of experiments, the floating cell trays were replaced with layers of floating perlite and the latter method was also tested for non-rooted shoots obtained in vitro in the multiplication stage or rooted plantlets obtained in the rooting stage. Direct ex vitro rooting in floating perlite was successful in ‘Tayberry’ (Rubus fruticosus × Rubus idaeus) (78.12% rooting); Amelanchier canadensis, Rubus fruticosus ‘Chester’, Rubus idaeus ‘Erntesegen’, Vaccinium macrocarpon, and Vaccinium corymbosum with rooting percentages above 80%; and Rubus idaeus ‘Willamette’ and Rosa hybrida ‘Cristiana’ with rooting percentages above 80%.

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