scholarly journals Application of molecular markers in sunflower breeding

Genetika ◽  
2007 ◽  
Vol 39 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Dejana Saftic-Pankovic
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Inbred Lines ◽  
Vegetable Crops ◽  
Large Variability ◽  
Parental Lines ◽  
Early Phases ◽  
Selection Of

The results of the application of molecular markers in sunflower breeding obtained in the Institute of Field and Vegetable Crops in the last decade are reviewed. Our results on genetic distance (GD=7-75%) between sunflower inbred lines obtained with RAPD and SSR markers, indicate large variability and provide important information for the selection of parental lines for future crosses. Interspecific hybridization is often used in sunflower breeding. As only some populations of H. giganteus and H. maximiliani are resistant to sunflower diseases, the investigation of genetic variability in/between two species is of interest. The results obtained with SSR markers are presented. The successful hybridization between H. rigidus and H. annuus was confirmed with RAPD markers, and the variability between F1 and BC1F1 plants is discussed. Desirable alleles and haplotypes can be detected with molecular markers both in early phases of plant development and in early phases of the production of improved lines, which reduces or completely eliminates the large number of testing cycles for desirable phenotypes. CAPS markers for resistance to downy mildew, that can be used in marker assisted selection are presented. .

scholarly journals Linkage of RAPD and SSR Markers to Thorniness and Floricane Fruiting in Blackberry

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1147B-1147
Author(s):  
Eric Stafne ◽  
John Clark ◽  
Kim Lewers
Keyword(s):  
Molecular Markers ◽  
Random Sample ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Marker Assisted Selection ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Sequence

Molecular markers have been used previously to identify linkages to important traits of interest. In this study two marker types, randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR), were used to find molecular markers linked to two morphological traits in blackberry (Rubus L. subgenus Rubus). Thorniness and floricane fruiting are both qualitative, recessive traits that are inherited tetrasomically. A cross of `Prime-Jim'® × `Arapaho' was made to create a population that segregated for the two traits. A random sample of 98 plants from a population of 200 were assayed to find molecular markers that co-segregate with the two traits. Three putative markers were identified for the floricane fruiting trait (two SSRs and one RAPD; χ2 = 4.09 to 9.99, P < 0.001 to 0.043). Five potential RAPD markers were found for the thorny trait (χ2 = 3.88 to 10.23, P < 0.001 to 0.048). Identification of markers linked to these traits could potentially be useful in marker-assisted selection.

Analisis Keragaman Genetik 28 Nomor Koleksi Kakao (Theobroma cacao L.) Berdasarkan Marka SSR

2017 ◽  
Vol 4 (1) ◽  
pp. 13 ◽  
Author(s):  
Ilham Nur ardhi Wicaksono ◽  
Rubiyo Rubiyo ◽  
Dewi Sukma ◽  
Sudarsono Sudarsono
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Plant Molecular Biology ◽  
Average Value ◽  
Germplasm Collections ◽  
Ctab Method ◽  
Parental Lines ◽  
Biology Laboratory ◽  
Superior Clones ◽  
Selection Of

<em>Analysis of genetic diversity of cacao germplasm collections using molecular markers has an important role in the assembly of new superior clones. The availability of commercial and superior local clones could increase the success of new superior clones’ assembly. Hence, the genetic diversity analysis of these materials needs to be done. The study was aimed to analyze genetic diversity of 28 cacao collections based on SSR markers that would be useful for selection of parental lines. The research was conducted in the Integrated Laboratory, Indonesian Industrial and Beverage Crops Research Institute, Sukabumi, and Plant Molecular Biology laboratory, Bogor Agricultural University, from November 2015 to May 2016.</em> <em>Analysis of genetic diversity was conducted using 28 cacao clones (13 superior local clones and 15 commercial clones). DNA was extraction using CTAB method, which then amplified by PCR technique using 20 SSR primers. The result showed that all SSR markers used in this study were polymorphic with an average value of PIC was high (57%). Phylogenetic tree constructed using DARwin program version 6.05 is divided into 3 major groups, which placed commercial and superior local clones together in each group. Superior local clones observed herein might have close relationships with commercial clones that have long been cultivated in Indonesia. Furthermore, some cacao clones could potentially be parental lines because they had high genetic distance. The results showed that SSR markers are powerful tools to determine potential parental lines, which is expected to increase the chances of heterosis in their progenies.</em>

scholarly journals Comparative analysis of genetic diversity among the maize inbred lines (Zea mays L.) obtained by RAPD and SSR markers

2008 ◽  
Vol 51 (1) ◽  
pp. 183-192 ◽  
Author(s):  
Silvia Graciele Hülse de Souza ◽  
Valéria Carpentieri-Pípolo ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Paulo Maurício Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Pedigree Data ◽  
Dice Coefficient ◽  
Quantitative Characters ◽  
Maize Inbred Lines ◽  
Ssr Primers ◽  
Selection Of

The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize the differences between the quantitative characters. Because they assess the distinct regions of the genome, the selection of one or other marker would depend on the characteristics of the material used and the objectives of the project.

scholarly journals Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Marker Assisted Selection ◽  
Inbred Lines ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Oleic ◽  
Low Linolenic ◽  
Selection Of

DEVELOPING MOLECULAR MARKERS FOR MARKER ASSISTED SELECTION OF FIRE BLIGHT RESISTANT APPLE SEEDLINGS

2007 ◽  
pp. 117-122 ◽  
Author(s):  
A. Peil ◽  
M.-V. Hanke ◽  
H. Flachowsky ◽  
K. Richter ◽  
T. Garcia ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Marker Assisted Selection ◽  
Fire Blight ◽  
Selection Of

scholarly journals Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

2007 ◽  
pp. 100-104
Author(s):  
Stella Molnár ◽  
Zsuzsanna Galbács ◽  
Gábor Halász ◽  
Sarolta Hoffmann ◽  
Anikó Veres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Pcr Rflp ◽  
Muscadinia Rotundifolia ◽  
Selection Of ◽  
Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

scholarly journals Marker-assisted selection using ridge regression

2000 ◽  
Vol 75 (2) ◽  
pp. 249-252 ◽  
Author(s):  
JOHN C. WHITTAKER ◽  
ROBIN THOMPSON ◽  
MIKE C. DENHAM
Keyword(s):  
Linear Regression ◽  
Ridge Regression ◽  
Marker Assisted Selection ◽  
Quantitative Traits ◽  
Selection Response ◽  
Inbred Lines ◽  
Response To Selection ◽  
Satisfactory Method ◽  
The Mean ◽  
Selection Of

In crosses between inbred lines, linear regression can be used to estimate the correlation of markers with a trait of interest; these marker effects then allow marker assisted selection (MAS) for quantitative traits. Usually a subset of markers to include in the model must be selected: no completely satisfactory method of doing this exists. We show that replacing this selection of markers by ridge regression can improve the mean response to selection and reduce the variability of selection response.

Effectiveness of predictive markers for marker assisted selection of pro-vitamin A carotenoids in medium-late maturing maize (Zea mays L.) inbred lines

2018 ◽  
Vol 79 ◽  
pp. 27-34 ◽  
Author(s):  
Sara Gebremeskel ◽  
Ana Luísa Garcia-Oliveira ◽  
Abebe Menkir ◽  
Victor Adetimirin ◽  
Melaku Gedil
Keyword(s):  
Zea Mays ◽  
Vitamin A ◽  
Marker Assisted Selection ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Predictive Markers ◽  
Selection Of

scholarly journals PRELIMNARY RESULTS ON MARKER ASSISTED SELECTION FOR DISEASE RESISTANCE IN COMMON WHEAT (TRITICUM AESTIVUM)

1970 ◽  
Vol 62 ◽  
Author(s):  
C. Botez ◽  
Monica Iuoraş ◽  
P. Raica ◽  
N. N. Saulescu
Keyword(s):  
Triticum Aestivum ◽  
Disease Resistance ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Issr Markers ◽  
Resistant Lines ◽  
Selection For

Our attempts to find RAPD markers linked with resistance genes to Septoria and Tilletia relying on co-segregation and to test the SSR markers, identified in literature, in order to select bread wheat resistant to Septoria and Tilletia are presented. Several candidate RAPD markers possibly linked with Septoria resistance genes and fewer linked with Tilletia resistance genes were identified. Some RAPD and SSR markers were specific for some resistant lines. These markers, probably, marked different resistance genes in different lines. It seems that some Septoria resistance genes came from rye genome because these genes were marked with some primers specific for rye genome. We found segregation for these markers in several lines, indicating that the analyzed lines were not completely stabilized. The ISSR markers used could not discriminate the resistant from susceptible genotypes to Septoria and Tilletia.

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