scholarly journals The Role of Soluble Sugars in Vase Solutions during the Vase Life of Eustoma grandiflorum

HortScience ◽  
2013 ◽  
Vol 48 (2) ◽  
pp. 222-226
Author(s):  
Ya-Ching Chuang ◽  
Yao-Chien Alex Chang
Keyword(s):  
Sucrose Solution ◽  
Sucrose Concentration ◽  
Soluble Sugars ◽  
Flower Senescence ◽  
Vase Life ◽  
Cut Flowers ◽  
Eustoma Grandiflorum ◽  
Flower Opening ◽  
Stems And Leaves

The vase life of Eustoma cut flowers can be extended by adding sugars to the vase solution, but the exact role of sugars and how they are translocated in tissues are not clear. Thus, we observed the preserving effect of different sugars in vase solutions on Eustoma and compared sugar concentrations in vase solutions and in the flowers as well as stems and leaves of cut flowers in a solution containing 200 mg·L−1 8-hydroxyquinoline sulfate (8-HQS) with and without 20 g·L−1 sucrose during different flowering stages. Inclusion of glucose, fructose, or sucrose in the vase solution extended the vase life of cut flowers with no significant differences among sugar types. During flower opening, the concentration of added sucrose in the vase solution dropped, and the fresh weight (FW), glucose concentration, and sucrose concentration of flowers in sucrose solutions increased, whereas flowers in solutions without sucrose had lower FW and glucose concentrations. During flower senescence, sugar concentration in the vase solution did not change much, but the FW and sucrose concentrations in all flowers declined, although the FW of sucrose-treated flowers fell more slowly. For stems and leaves in the sucrose solution, sugar concentrations increased during the first 7 days with only glucose slightly declining during senescence, whereas the FW was maintained during the entire vase life. In contrast, FWs of those in the solution without sucrose gradually declined. In conclusion, sucrose in the vase solution promoted flower opening and maintained the water balance of Eustoma cut flowers. Glucose and fructose also extended the vase life, likely in similar ways.

scholarly journals Polyamines accentuate vase life by augmenting antioxidant system in cut spikes of Consolida ajacis (L.) Schur.

2021 ◽  
Vol 27 (4) ◽  
pp. 495-504
Author(s):  
Aehsan ul Haq ◽  
Sumira Farooq ◽  
Mohammad Lateef Lone ◽  
Shazia Parveen ◽  
Foziya Altaf ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Postharvest Quality ◽  
Flower Senescence ◽  
Vase Life ◽  
Distilled Water ◽  
Oxygen Species ◽  
Cut Flowers ◽  
Postharvest Senescence

Abstract Postharvest senescence is one of the crucial challenges limiting the marketability of cut flowers. Pertinently, recent investigations implicate extensive role of polyamines in regulation of flower senescence. The present study was envisaged to test the efficacy of poylamines in preserving the postharvest quality of Consolida ajacis (C. ajacis) cut spikes. The cut spikes of C. ajacis were subjected to various treatments of polyamines viz, 4 mM Spermine (SPM), 6mM Putrescine (PUT) and 6 mM Spermidine (SPD). A separate set of spikes held in distilled water represented the control. Our results authenticate a significant improvement in vase life of cut spikes of C. ajacis as compared to control. The increment in vase life was commensurate with the higher concentration of sugars, proteins and phenols in the tepal tissues. Polyamines amplified the activity of various antioxidant enzymes viz, superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) to overcome the deleterious effects of reactive oxygen species (ROS). The membrane outflow of tepal tissues was profoundly reduced due to attenuated lipoxygenase (LOX) activity. These findings reveal conspicuous role of polyamines particularly SPM in modulation of flower senescence in cut spikes of C. ajacis.

scholarly journals Effects of Hormone Treatments on Cut Flower Opening and Senescence in Wintersweet (Chimonanthus praecox)

HortScience ◽  
2015 ◽  
Vol 50 (9) ◽  
pp. 1365-1369 ◽  
Author(s):  
Shunzhao Sui ◽  
Jianghui Luo ◽  
Daofeng Liu ◽  
Jing Ma ◽  
Weiting Men ◽  
...  
Keyword(s):  
Vase Life ◽  
Cut Flower ◽  
Related Gene ◽  
Cut Flowers ◽  
Flower Opening ◽  
Delayed Senescence ◽  
Ethylene Action ◽  
Garden Plant ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox) is a woody garden plant with fragrant flowers, which blooms in deep winter. The vase life of fresh cut flowers is 8–9 days. We applied ethylene and 1-methylcyclopropene (1-MCP; an ethylene action inhibitor) to test the role of ethylene in flower opening and senescence. In addition, abscisic acid (ABA), gibberellic acid (GA3), two cytokinins, 6-benzylaminopurine (6-BA), and zeatin (ZT) were also applied. The expression pattern of CpSRG1, a senescence-related gene, was analyzed. Ethylene treatment accelerated flower opening and senescence, decreasing vase life by 2.1 days. It also decreased flower break strength, indicating the induction of abscission. 1-MCP slowed opening, delayed senescence, and prolonged vase life by 2.6 days. Ethylene dramatically induced the expression of the CpSRG1 gene, while 1-MCP suppressed it. ZT promoted flower opening and increased vase life by 1.6 days. It suppressed the expression of CpSRG1. 6-BA, GA3, or ABA had no significant effect on flower opening and senescence of wintersweet.

scholarly journals Changes in carbohydrates associated with senescence of cut gladiolus spikes under pulsing and wet cold storage durations

2020 ◽  
Vol 28 (s1) ◽  
pp. 41-53
Author(s):  
K.J. Chore ◽  
M. Mwangi ◽  
S.M. Karori ◽  
A.M. Kibe
Keyword(s):  
Cold Storage ◽  
Sucrose Solution ◽  
Soluble Sugars ◽  
Vase Life ◽  
Cut Flowers ◽  
Storage Duration ◽  
Total Soluble Sugars ◽  
Research And Teaching ◽  
Significant Difference ◽  
Total Starch

Several biochemical and metabolic changes are associated with senescence of cut gladioli, particularly in relation to quality characteristics, including dynamism of carbohydrates. The objective of this study was to evaluate the effect of pulsing and wet cold storage on the starch and sugar biomarkers on cut Gladiolus (Gladiolus grandiflorus L. cv. Fado) vase quality. Pulsing treatments of 600-ppm 8-hydroxyquinoline sulphate, plus 5% sucrose solution, versus distilled water, were administered prior to wet cold storage durations of 0 - 5 days, on the cut Gladiolus grown from corms. This was done in the open field at the Horticulture Research and Teaching Field, Egerton University in Kenya, during two successive seasons. There was a significant difference (P <0.01) in total soluble sugars (TSS) and total starch of spikes pulsed with 600 ppm 8 - HQS + 5% sucrose, compared with the control, during the third day in the vase life of the cut flowers. Prolonged vase life of cut spikes was associated with a decrease in total soluble sugars and increase in total starch, as influenced by pulsing and wet storage duration up to 4 days.

scholarly journals Tree Bark Phenols Regulate the Physiological and Biochemical Performance of Gladiolus Flowers

Processes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 71 ◽  
Author(s):  
Hosam O. Elansary
Keyword(s):  
Water Content ◽  
Microbial Growth ◽  
Antioxidant Activities ◽  
Soluble Sugars ◽  
Tree Bark ◽  
Flower Senescence ◽  
Bark Extract ◽  
Plant Residues ◽  
Vase Life ◽  
Gas Exchange Parameters

The postharvest physiology of cut flowers is largely dependent on vase life, which is the maximum number of days before flower senescence. The use of tree bark extracts (major forest plant residues), as an eco-friendly and natural antioxidant preservative in holding solutions, is a novel tool for extending flower longevity. The morphological, physiological, biochemical, and genetic responses of Gladiolus grandiflorus cut spikes to Magnolia acuminata and Taxus cuspidata bark extracts as additives in holding solutions were investigated. G. grandiflorus subjected to bark extracts as well as catechin and protocatechuic acid (main phenols) displayed significant increased longevity (up to 18 days), an increased number of open florets, and increased floret fresh weight. Increases in the relative water content, leaf chlorophyll, carotenoids, soluble sugars, and protein content were observed in addition to a reduction in microbial growth in the cut spikes. Gas exchange parameters were higher in the bark extract treatments than in the controls. Higher antioxidant activities were detected and associated with increased superoxide dismutase and catalase enzyme activities and reduced H2O2 accumulation. The bark extract treatments associated with reduced expression of GgCyP1 (produces cysteine protease) and increased expression of both GgDAD1 (defends against apoptotic activity) and GgEXPA1 (regulates petal expansion). Several mechanisms were implicated in these effects, including maintenance of water content, enhanced management of reactive oxygen species (ROS), increased sugar and protein composition, and control of microbial growth. Thus, bark extracts and isolated phenols could be developed as an eco-friendly, non-toxic, and cost-effective natural preservative for cut gladiolus flowers.

scholarly journals Effects of Daily Harvest Time on Postharvest Longevity, Water Relations, and Carbohydrate Status of Selected Specialty Cut Flowers

HortScience ◽  
2014 ◽  
Vol 49 (3) ◽  
pp. 297-305
Author(s):  
Iftikhar Ahmad ◽  
John M. Dole ◽  
Frank A. Blazich
Keyword(s):  
Vase Life ◽  
Harvest Time ◽  
Cut Flowers ◽  
Weight Changes ◽  
Eustoma Grandiflorum ◽  
Relative Water ◽  
Leaf Relative Water Content ◽  
Postharvest Longevity ◽  
Carbohydrate Status ◽  
Time Of Harvest

Effects of harvest time (morning, noon, or afternoon) on water uptake, fresh weight changes, termination symptoms, leaf relative water content (LRWC), carbohydrate status, and vase life of cut ‘ABC Purple’ lisianthus (Eustoma grandiflorum Salisb.), ‘Double Eagle’ African Gold Coin Series marigold (Tagetes erecta L.), and ‘Deep Red’ Benary’s Giant Series zinnia (Zinnia elegans Jacq.) were studied. For stems of lisianthus harvested and then stored in the dark with the basal ends in water for 2 weeks at 3 ± 1 °C, those harvested at noon (1200 hr to 1300 hr) or in the afternoon (1700 hr to 1800 hr) had longer vase life compared with stems harvested in the morning (0700 hr to 0800 hr). However, stems of lisianthus evaluated without storage had no differences in vase life. Stems of marigold harvested in the afternoon had longer vase life than morning- or noon-harvested stems. Time of harvest had no effect on cut flower longevity of zinnia. However, vase life was considerably shorter for stems of all species when tested after 2 weeks storage compared with freshly harvested stems. Stems of zinnia harvested at noon had lower LRWC than morning- or afternoon-harvested stems. Marigold stems harvested in the afternoon and evaluated without storage had lowest LRWC on Day 7 of vase life. Harvest time or storage did not influence LRWC of lisianthus. Stems of marigold and lisianthus harvested at noon or in the afternoon had higher levels of carbohydrates compared with morning-harvested stems, whereas freshly harvested stems had higher concentrations of glucose and sucrose, which decreased during storage or the vase period. Sucrose concentrations varied more significantly among various tissues than other sugars presumably as a result of translocation during vase life. In summary, carbohydrate status of stems harvested at different times of the day varied greatly and affected postharvest longevity of cut marigold and lisianthus, but not zinnia.

scholarly journals Genotypic Variation in the Postharvest Performance and Ethylene Sensitivity of Cut Rose Flowers

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 790-796 ◽  
Author(s):  
Andrew J. Macnish ◽  
Ria T. Leonard ◽  
Ana Maria Borda ◽  
Terril A. Nell
Keyword(s):  
Natural Variation ◽  
No Reduction ◽  
Genotypic Variation ◽  
Ornamental Plants ◽  
Postharvest Quality ◽  
Vase Life ◽  
Cut Flowers ◽  
Flower Opening ◽  
Ethylene Sensitivity ◽  
Duration Of Protection

Natural variation in the postharvest quality and longevity of ornamental plants can often be related to differences in their response to ethylene. In the present study, we determined the postharvest performance and ethylene sensitivity of cut flowers from 38 cultivated Hybrid Tea rose genotypes. The vase life of the cultivars varied considerably from 4.5 to 18.8 days at 21 °C. There was also substantial variation in the degree of flower opening among genotypes. Exposure to 1 μL·L−1 ethylene for 24 h at 21 °C reduced the longevity of 27 cultivars by 0.8 to 8.4 days (18% to 47%) by accelerating petal wilting and abscission. Ethylene treatment also significantly reduced rates of flower opening in 17 sensitive cultivars and in six cultivars that showed no ethylene-related reduction in vase life. Five cultivars showed no reduction in vase life or flower opening in response to ethylene exposure. Pre-treating stems with 0.2 mm silver thiosulfate liquid or 0.9 μL·L−1 1-methylcyclopropene (1-MCP) gas for 16 h at 2 °C reduced the deleterious effects of ethylene. The release of 1-MCP from two sachets containing EthylBloc™ into individual shipping boxes also protected flowers against ethylene applied immediately after a 6-d commercial shipment. The duration of protection afforded by the 1-MCP sachet treatment was greatest when flowers were maintained at low temperature.

scholarly journals Effect of PGRs on Antioxidant Activity and Phytochemical in Delay Senescence of Lily Cut Flowers

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1704
Author(s):  
Sabir Aziz ◽  
Adnan Younis ◽  
Muhammad Jafar Jaskani ◽  
Rashid Ahmad
Keyword(s):  
Vase Life ◽  
Cut Flower ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Chlorophyll Contents ◽  
Spad Value ◽  
Maximum Change ◽  
Preservative Solutions

The short vase life is the major problem in the cut flower industry. This study was conducted to evaluate the role of different vase solutions and oils in enhancing the quality and vase life of lily cut flowers. Salicylic acid (SA; 300 mg L−1), citric acid (CA; 300 mg L−1), gibberellic acid (GA; 100 mg L−1), and clove oil (200 mg L−1) were used as vase solutions. These treatments were applied after pulsing with preoptimized sucrose 5%. It was found that SA (300 mg L−1) + sucrose (5%) improved the performance of cut flowers, which further increased the longevity of all tested lily cultivars up to eight days and the longest vase life by 17.6 days. The maximum change in fresh weight (5.60 g), increase in chlorophyll contents (3.2 SPAD value), highest protein content (6.1 mg g−1 FW), and increase in the activities of superoxide dismutase (SOD) (51.0 U g−1 protein), catalase (CAT) (36.3 U g−1 protein), and peroxidase (POD) (41.6 U g−1 protein), were recorded with the CA (300 mg L−1) + sucrose 5%. Among the cultivars, “Zambesi” performed best compared to “Sorbonne” and “Caesars”. The maximum anthocyanin contents (198%) were recorded in “Caesars”. In conclusion, among the different preservative solutions, SA performed best to prolong the vase life and quality of lily cut flowers.

scholarly journals Postharvest quality of Aster ericoides after treatment with silver thiosulphate and sucrose

2018 ◽  
Vol 48 (12) ◽  
Author(s):  
Kathia Fernandes Lopes Pivetta ◽  
Claudia Fabrino Machado Mattiuz ◽  
Regina Ferreira de Melo ◽  
Renata Gimenes ◽  
Gustavo de Nobrega Romani ◽  
...  
Keyword(s):  
Polynomial Regression ◽  
Sucrose Solution ◽  
Fresh Mass ◽  
Postharvest Quality ◽  
Vase Life ◽  
Cut Flowers ◽  
Silver Thiosulphate ◽  
Randomized Design ◽  
Aster Ericoides ◽  
Relative Water

ABSTRACT: Aster (Aster ericoides L.) is a flower crop of North American origin, which belongs to the family Asteraceae. This plant presents capitulum-type inflorescences, widely used as cut flowers. In Brazil, it has been grown only recently, but still has a high expansion potential. In floriculture, pulsing and conditioning solutions are often used to extend the vase life of cut flowers. The present study aimed to evaluate the influence of silver thiosulphate (STS) pulsing and sucrose solutions on the postharvest life of A. ericoides stems. The experiment was carried out under a complete randomized design with treatments arranged in a 2x5 factorial scheme. Treatments consisted of flower stems treated with STS pulsing for 30 minutes, combined with four sucrose concentrations (5, 10, 15, and 20%) for six hours, plus a control (without treatment). Each plot contained four replicates of 10 stems, totalizing 400 stems. The following variables were daily: vase life, fresh mass, and relative water content (RWC). For pulsing data, means were compared by the Tukey’s test (p≥5%). Yet for sucrose concentrations, means were submitted to a polynomial regression to verify the behavior of variables as a function of concentrations increases. Likewise, each treatment was evaluated for fresh mass and RWC variations over time by a polynomial regression. Results indicated no effect of STS pulsing (2mM) on the vase-life length of aster stems. In contrast, the sucrose solution at 10% extended the vase life of stems by two days, decreased fresh mass loss, and helped maintain water balance in stems.

scholarly journals The Effect of a Sugar-Containing Preservative on Senescence-Related Processes in Cut Clematis Flowers

2018 ◽  
Vol 47 (2) ◽  
pp. 432-440 ◽  
Author(s):  
Julita RABIZA-ŚWIDER ◽  
Ewa SKUTNIK ◽  
Agata JĘDRZEJUK
Keyword(s):  
Proteolytic Activity ◽  
Cysteine Protease ◽  
Reducing Sugars ◽  
Soluble Proteins ◽  
Flower Senescence ◽  
Vase Life ◽  
Free Proline ◽  
Cut Flower ◽  
Cut Flowers ◽  
Life Itself

Clematis is a new species grown as cut flower, whose vase life is variable and cultivar-depended. Little is known about senescence of its cut flowers and their response to flower preservatives. The aim of the study was to evaluate the effect of a preservative (standard preservative SP, 8-hydroxyquinoline citrate plus sucrose) or a biocide solution (8-HQC) on certain senescence-related processes in cut clematis flowers. Analyses were done immediately after harvest and at the end of the vase life when control flowers held in water were wilting. A possible relationship between senescence parameters and the vase life of clematis taxa was also sought. As in most cut flowers, the contents of reducing sugars and soluble proteins in clematis petals decreased during the vase life while the proteolytic activity, including that of the cysteine protease, increased and was accompanied by accumulation of free proline and ammonium. Cut flower longevity in cultivars under study was not associated with the initial levels of reducing sugars, soluble proteins or free proline. Neither was the initial proteolytic activity or its increase during vase life related to the vase life itself: cultivars having comparable life spans differed dramatically in the initial and final proteolytic activities. Both solutions containing 8-HQC significantly affected the senescence-related processes and flowers held in them had more soluble proteins and lower proteolytic activity (total, and that of the cysteine protease) than control flowers held in water. Approximately a twofold increase in reducing sugars was observed in flowers held in SP relative to those held in water or in 8-HQC while the accumulation of free proline and ammonium was limited in their petals. This suggests a regulating action of exogenous sugar in senescence of clematis flowers. However, the delay of senescence produced by the preservative was not always associated with a longer vase life in any given cultivar. Further studies are needed to elucidate the role of sugar in clematis flower senescence.

scholarly journals Effect of 8-Hydroxyquinoline Citrate, Sucrose and Peroxidase Inhibitors on Vase Life of Lisianthus (EUSTOMA GRANDIFLORUM L.) Cut Flowers

2014 ◽  
Vol 22 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Kamaladin Sharifzadeh ◽  
Moazzam Hassanpour Asil ◽  
Zeynab Roein ◽  
Muhammad Sharifzadeh
Keyword(s):  
Weight Loss ◽  
Superoxide Dismutase ◽  
Water Uptake ◽  
The Other ◽  
Life Extension ◽  
Vase Life ◽  
Anthocyanin Content ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Eustoma Grandiflorum

AbstractCut lisianthus flowers have a short vase life, possibly due to blockage of xylem vessels. The effect of 8-hydroxyquinoline citrate, sucrose and peroxidase inhibitors on delaying senescence and extending vase life of cut lisianthus flowers was tested. The peroxidase inhibitors used in this experiment were catechol (CH) (5, 10, 15 mM) and p-phenylenediamine (PD) (5, 10, 15 mM). All vase solutions contained 200 mg-dm-3 8-hydroxyquinoline citrate (8-HQC) and 3% sucrose. 10 mM CH treatment was the most effective for vase life extension (13.3 days), increasing water uptake, and delaying fresh weight loss. The vase solution containing 10 mM CH significantly increased superoxide dismutase (SOD) and decreased peroxidase (POD) activities. Similarly, 10 mM PD increased anthocyanin content more than the other treat–ments. Protein degradation was significantly delayed by application of 5 mM PD. The malondialdehyde (MDA) accumulation was reduced when CH at 5 mM and PD in 5 and 15 mM were added to the vase solution. Results indicated that peroxidase inhibitors in combination with 8-HQC and sucrose increase vase life of lisianthus by improving water uptake and delaying fresh weight loss.

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