scholarly journals Bulked-segregant Analysis Identified a Putative Region Related to Short Internode Length in Melon

HortScience ◽  
2019 ◽  
Vol 54 (8) ◽  
pp. 1293-1298 ◽  
Author(s):  
Taifeng Zhang ◽  
Jiajun Liu ◽  
Shi Liu ◽  
Zhuo Ding ◽  
Feishi Luan ◽  
...  
Keyword(s):  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Genomic Region ◽  
Internode Length ◽  
Chromosome 7 ◽  
Stem Length ◽  
Whole Genome Analysis ◽  
Short Internode ◽  
Breeding Goal ◽  
Caps Markers

Short internode length (SIL) is one of the most commercially and important traits in melon varieties (Cucumis melo L.). SIL can result in a compact vining type that promotes concentrated fruit in high-density crops, leading to greater use of light resources for photosynthesis and greater yield per unit area. In our study, two parental melon lines ‘M1-32’ (P1, standard vine) and ‘X090’ (P2, short internodes), and their F1, F2, BC1P1, and BC1P2 progenies were evaluated after being grown in plastic greenhouse conditions in 2017 and 2018. Main stem length (MSL) and internode length (IL) of six melon generations indicated that a single recessive gene (MD7) controlled dwarfism in the ‘X090’ melon line. Whole-genome analysis revealed a genomic region harboring the candidate dwarfism gene on chromosome 7. Six polymorphic cleaved amplified polymorphic sequence (CAPS) markers from chromosome 7 were used to construct a genetic linkage that spanned 30.28 cM. The melon dwarfing locus MD7 responsible for SIL was positioned between markers M7-4 and M7-5, with 3.16 cM of flanking distance. The CAPS markers M7-4 and M7-5 developed have the potential to accelerate the development of dwarf melon varieties, especially in situations when dwarf genotypes are an important breeding goal using marker-assisted selection.

Genetic map of lps3: a new short petiole gene in soybeans

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Tae-Hwan Jun ◽  
Sung-Taeg Kang
Keyword(s):  
Ssr Marker ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Genomic Region ◽  
Snp Markers ◽  
High Yield ◽  
Plant Canopy ◽  
Single Recessive Gene ◽  
Genomic Location ◽  
Soybean Plants

The short petiole trait is valuable for the development of plant ideotypes with high yield by improving the plant canopy. The soybean breeding line SS98206SP has shown extremely short petioles in the greenhouse and fields. A new single recessive gene designated as lps3 confers the short petiole trait in SS98206SP. The objectives of this study were to map the short petiole gene in SS98206SP with PCR-based markers. In total, 187 F2 plants and their F2:3 families from a cross between the short petiole line SS98206SP and the long petiole cultivar ‘Taekwang’ along with the two parental lines were evaluated for their petiole lengths in a greenhouse. An SSR marker from each 10-cM section of a consensus soybean map was selected for bulked segregant analysis (BSA) to identify the tentative genomic location of the gene. The BSA technique was useful to localize the gene to a genomic region in the soybean linkage group F (chromosome 13). A linkage map with six SSR and two SNP markers flanking the gene was constructed. We positioned the gene between two SSR markers, Sat_234 and Sct_033, at distances of 8.5 and 3.5 cM from the marker, respectively. The makers flanking the gene (lps3) were located within 3–4 cM of the gene. These markers will be useful for maker-assisted selection in the development of new ideotype soybean plants.

scholarly journals Inheritance of a Short-internode Mutant of `Mainstream' Muskmelon

HortScience ◽  
1990 ◽  
Vol 25 (10) ◽  
pp. 1274-1275 ◽  
Author(s):  
Dean E. Knavel
Keyword(s):  
Cucumis Melo ◽  
Recessive Gene ◽  
Internode Length ◽  
Breeding Lines ◽  
Segregation Data ◽  
Short Internode ◽  
Short Internodes

A short-internode mutant of `Mainstream' muskmelon (Cucumis melo L.), designated Main Dwarf, was crossed with 13 normal and six short-internode cultivars or breeding lines. Regardless of whether Main Dwarf was crossed with a normal or short-internode line, the F1 family was normal for internode length. From crosses of Main Dwarf with normal lines, the F2 families segregated in a 3 normal: 1 short-internode ratio and the families from backcrosses to Main Dwarf segregated 1 normal: 1 short internode. Crosses of Main Dwarf with short-internode lines produced F2 families that segregated in a 9 normal: 7 short internode ratio and families from backcrosses to Main Dwarf that segregated 1 normal: 1 short internode. The segregation data from crosses of Main Dwarf with normal lines indicate that Main Dwarf has a recessive gene that conditions short internode. The segregation data from crosses of Main Dwarf with short-internode lines (five conditioned by si-1 and one conditioned by si-2) indicate that the recessive gene for short internode in Main Dwarf is not allelic to si-1 or si-2. The gene for short internodes in Main Dwarf is designated si-3.

scholarly journals A mutation in LacDWARF1 results in a GA-deficient dwarf phenotype in sponge gourd (Luffa acutangula)

2021 ◽  
Author(s):  
Gangjun Zhao ◽  
Caixia Luo ◽  
Jianning Luo ◽  
Junxing Li ◽  
Hao Gong ◽  
...  
Keyword(s):  
Gene Annotation ◽  
Recessive Gene ◽  
Genomic Region ◽  
Dwarf Mutant ◽  
Rna Seq ◽  
Dwarf Phenotype ◽  
Sponge Gourd ◽  
Response To Stress ◽  
Luffa Acutangula ◽  
Generation Sequencing

Abstract Key message A dwarfism gene LacDWARF1 was mapped by combined BSA-Seq and comparative genomics analyses to a 65.4 kb physical genomic region on chromosome 05. Abstract Dwarf architecture is one of the most important traits utilized in Cucurbitaceae breeding because it saves labor and increases the harvest index. To our knowledge, there has been no prior research about dwarfism in the sponge gourd. This study reports the first dwarf mutant WJ209 with a decrease in cell size and internodes. A genetic analysis revealed that the mutant phenotype was controlled by a single recessive gene, which is designated Lacdwarf1 (Lacd1). Combined with bulked segregate analysis and next-generation sequencing, we quickly mapped a 65.4 kb region on chromosome 5 using F2 segregation population with InDel and SNP polymorphism markers. Gene annotation revealed that Lac05g019500 encodes a gibberellin 3β-hydroxylase (GA3ox) that functions as the most likely candidate gene for Lacd1. DNA sequence analysis showed that there is an approximately 4 kb insertion in the first intron of Lac05g019500 in WJ209. Lac05g019500 is transcribed incorrectly in the dwarf mutant owing to the presence of the insertion. Moreover, the bioactive GAs decreased significantly in WJ209, and the dwarf phenotype could be restored by exogenous GA3 treatment, indicating that WJ209 is a GA-deficient mutant. All these results support the conclusion that Lac05g019500 is the Lacd1 gene. In addition, RNA-Seq revealed that many genes, including those related to plant hormones, cellular process, cell wall, membrane and response to stress, were significantly altered in WJ209 compared with the wild type. This study will aid in the use of molecular marker-assisted breeding in the dwarf sponge gourd.

scholarly journals Evaluation of Chlorimuron as a Growth Regulator for Peanut

1995 ◽  
Vol 22 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Wayne E. Mitchem ◽  
Alan C. York ◽  
Roger B. Batts
Keyword(s):  
Growth Regulator ◽  
Vegetative Growth ◽  
Lateral Branch ◽  
Internode Length ◽  
Main Stem ◽  
Stem Length ◽  
Vine Growth

Abstract Chlorimuron was evaluated as a growth regulator on peanut. Treatments included chlorimuron at a total of 8.8 g ai/ha applied once at 60,75, or 90 d after emergence (DAE) or in equal portions applied twice at 60 and 75, 60 and 90, or 75 and 90 DAE or three times at 60, 75, and 90 DAE. Daminozide at 950 g ai/ha applied 75 DAE was included as a comparison. In a year with excessive vine growth, daminozide and all chlorimuron treatments except 8.8 g/ha applied 90 DAE reduced cotyledonary lateral branch and main stem length at harvest 9 to 20 and 12 to 24%, respectively, due to suppression of internode length. Sequential applications of chlorimuron generally suppressed growth more than single applications. No improvement in row visibility at harvest was noted. In a dry year with limited vegetative growth, neither chlorimuron nor daminozide affected cotyledonary lateral branch or main stem length at harvest. Chlorimuron at 2.9 g/ha applied 60, 75, and 90 DAE reduced yield 18% at one of four locations; no other treatment affected yield. Chlorimuron at 8.8 g/ha applied 60 DAE or 4.4 g/ha applied 60 and 75 DAE reduced the percentage of fancy pods and extra large kernels at one or more locations. No treatment affected the percentage of total sound mature kernels. Results suggest chlorimuron has little to no potential for use as a growth regulator.

Prevalence of congenital absence of vas deferens and related genetic factors among male infertilities: A systematic review

2021 ◽  
Vol 30 (9) ◽  
pp. 59-67
Author(s):  
Dang Tuan Anh ◽  
Cao Tuan Anh ◽  
Nguyen Huy Hoang ◽  
Bui Thi Len ◽  
Dang Tien Truong ◽  
...  
Keyword(s):  
Systematic Review ◽  
Genetic Factors ◽  
Vas Deferens ◽  
Recessive Gene ◽  
Chromosome 7 ◽  
Congenital Absence ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
G Protein Coupled ◽  
Genetic Consultation

A systematic review was conducted to describe the prevalence of congenital absence of vas deferens (CAVD) and types of cystic fbrosis transmembrane conductance regulator (CFTR) gene mutation, Adhesion G-protein coupled receptor G2 (ADGRG2) mutation leading to CAVD among male infertilities aimed at providing comprehensive counseling in the treatment of male infertility. Information and data from eligible publications were searched and selected from the PubMed and Medline e-library from 2010 to 2020 by using 8 main keywords. The results showed that the prevalence of CAVD was approximately 0.1% in male and about 80% of congenital bilateral absences of the vas deferens (CABVD) patients had the CFTR mutation. These mutations were recessive gene abnormalities on the long arm of chromosome 7. Besides the mutation, recent researches showed the mutation of the ADGRG2 gene on X chromosome also led to CBAVD. It was approximately 10 - 20% of CBAVD and 60 - 70% of the congenital unilateral absence of the vas deferens (CUAVD) that was still not diagnosed genetically. In conclusion, the genetic consultation is necessary for CAVD patients before deciding to have a baby

Rapid mapping of a chlorina mutant gene cn-A1 in hexaploid wheat by bulked segregant analysis and single nucleotide polymorphism genotyping arrays

2019 ◽  
Vol 70 (10) ◽  
pp. 827 ◽  
Author(s):  
H. B. Jiang ◽  
N. Wang ◽  
J. T. Jian ◽  
C. S. Wang ◽  
Y. Z. Xie
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Single Nucleotide Polymorphism Array ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Mutant Gene ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Chlorophyll Metabolism ◽  
Specific Pcr ◽  
Leaf Mutant

The yellow–green leaf mutant can be exploited in photosynthesis and plant development research. A Triticum aestivum mutant with the chlorina phenotype, here called B23, was produced by treatment with the chemical mutagen sodium azide. This B23 mutant showed significantly lower chlorophyll content than wild-type Saannong33, and its chloroplast structure was abnormal. All its yield-related traits, except for the number of spikes per plant, were also significantly decreased. Genetic analysis confirmed that the mutant phenotype was controlled by a recessive gene, here designated cn-A1. Using bulked segregant analysis and the wheat 660K single nucleotide polymorphism array, the cn-A1 gene was mapped to chromosome 7AL, and 11 polymorphic markers were developed. Further analysis showed that cn-A1 was located in a 1.1-cM genetic region flanked by Kompetitive allele specific PCR (KASP) markers 660K-7A12 and 660K-7A20, which corresponded to a physical interval of 3.48 Mb in T. aestivum cv. Chinese Spring chromosome 7AL containing 47 predicted genes with high confidence. These results are expected to accelerate the process of cloning the cn-A1 gene and facilitate understanding of the mechanisms underlying chlorophyll metabolism and chloroplast development in wheat.

scholarly journals Mapping and Identifying a Candidate Gene Plr4, a Recessive Gene Regulating Purple Leaf in Rice, by Using Bulked Segregant and Transcriptome Analysis with Next-Generation Sequencing

2019 ◽  
Vol 20 (18) ◽  
pp. 4335 ◽  
Author(s):  
Ju Gao ◽  
Gaoxing Dai ◽  
Weiyong Zhou ◽  
Haifu Liang ◽  
Juan Huang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Anthocyanin Biosynthesis ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Leaf Traits ◽  
Anthocyanin Synthesis ◽  
Genetic Characteristics ◽  
Specific Organ ◽  
Flower Organs ◽  
Purple Leaf

The anthocyanin biosynthesis of rice is a major concern due to the potential nutritional value. Purple appears in various organs and tissues of rice such as pericarp, flower organs, leaves, leaf sheaths, internodes, ligules, apex, and stigma. At present, there are many studies on the color of rice pericarp, but the gene and mechanism of other organs such as leaves are still unclear, and the gene regulatory network of specific organ coloring has not been systematically understood. In this study, genetic analysis demonstrated that the purple leaf traits of rice were regulated by a recessive gene. The green leaf cultivar Y58S and purple leaf cultivar XianHongB were used to construct the mapping population. A set of near isogenicline (NIL) (BC3F1) was bred via crossing and back-crossing. The generations of BC3F2 appeared to separate four phenotypes, pl1, pl2, pl3, and pl4, due to the occurrence of a purple color in different organs. We constructed three bulked segregant analysis (BSA) pools (pl1–pl2, pl1–pl3, and pl1–pl4) by using the separated generations of BC3F5 and mapped the purple leaf gene plr4 to the vicinity of 27.9–31.1 Mb on chromosome 4. Subsequently, transcriptome sequencing (RNA-Seq) for pl3 and pl2 was used to analyze the differentially expressed genes in the localization interval, where 12 unigenes exhibited differential expression in which two genes (Os04g0577800, Os04g0616400) were downregulated. The two downregulated genes (Os04g0577800 and Os04g0616400) are possible candidate genes because of the recessive genetic characteristics of the purple leaf genes. These results will facilitate the cloning of plr4 and illustrate the molecular mechanisms of the anthocyanin synthesis pathway.

scholarly journals Genetic Analysis and Related Gene Primary Mapping of Heat Stress Tolerance in Cucumber Using Bulked Segregant Analysis

HortScience ◽  
2019 ◽  
Vol 54 (3) ◽  
pp. 423-428
Author(s):  
Min Wang ◽  
Wenrui Liu ◽  
Biao Jiang ◽  
Qingwu Peng ◽  
Xiaoming He ◽  
...  
Keyword(s):  
Heat Stress ◽  
Genetic Analysis ◽  
Candidate Genes ◽  
High Temperatures ◽  
Genomic Sequence ◽  
Bulked Segregant Analysis ◽  
Recessive Gene ◽  
Chromosome 1 ◽  
Isolation And Characterization ◽  
Indel Markers

Heat stress (HS) negatively influences plant development and growth, especially production and quality. Cucumber is a widely cultivated plant in the gourd family Cucurbitaceae that is often exposed to high temperatures during summer and protected cultivation. In this study, we performed whole-genome re-sequencing of two pools, one heat-tolerant and one heat-sensitive, of the F2 population derived from L-9 (heat-resistant) and A-16 (heat-sensitive). The genetic analysis showed that the heat resistance of L-9 cucumber seedlings was controlled by a single recessive gene. By combining bulked segregant analysis (BSA) technology, the crucial gene related to HS was preliminarily mapped to a 1.08-Mb region on chromosome 1. To fine-map the locus, Indel markers were designed according to the genomic sequence. Finally, the gene was narrowed to a 550-kb region flanked by two Indel markers, namely Indel-H90 and Indel-H224, that contained 56 candidate genes. Re-sequencing results indicated that 10 candidate genes among the 56 in the candidate region showed single base pair differences in the exons. Quantitative reverse-transcription polymerase chain reaction showed that 6 genes among the 10 candidate genes were significantly decreased when exposed to high temperatures. These results not only were useful for the isolation and characterization of the key genes involved in HS but also provided a basis for understanding the mechanism of heat tolerance regulation.

scholarly journals Influence of Day and Night Temperatures on Sweet Pepper Seedling Development

1996 ◽  
Vol 121 (4) ◽  
pp. 699-704 ◽  
Author(s):  
Yaping Si ◽  
Royal D. Heins
Keyword(s):  
Leaf Area ◽  
Dry Weight ◽  
Sweet Pepper ◽  
Internode Length ◽  
Stem Diameter ◽  
Stem Length ◽  
Shoot Dry Weight ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
The Difference

Sweet pepper (Capsicum annuum `Resistant Giant no. 4') seedlings were grown for 6 weeks in 128-cell plug trays under 16 day/night temperature (DT/NT) regimes from 14 to 26 °C. Seedling stem length, internode length, stem diameter, leaf area, internode and leaf count, plant volume, shoot dry weight (DW), seedling index, and leaf unfolding rate (LUR) were primarily functions of average daily temperature (ADT); i.e., DT and NT had similar effects on each growth or development parameter. Compared to ADT, the difference (DIF, where DIF = DT - NT) between DT and NT had a smaller but still statistically significant effect on stem and internode length, leaf area, plant volume, stem diameter, and seedling index. DIF had no effect on internode and leaf count, shoot DW, and LUR. The root: shoot ratio and leaf reflectance were affected by DT and DIF. Positive DIF (DT higher than NT) caused darker-green leaf color than negative DIF. The node at which the first flower initiated was related to NT. The number of nodes to the first flower on pepper plugs grown at 26 C NT was 1.2 fewer than those of plants grown at 14 °C NT.

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