scholarly journals Seed Developmental Temperature Regulation of Thermotolerance in Lettuce

1998 ◽  
Vol 123 (4) ◽  
pp. 700-705 ◽  
Author(s):  
Yu Sung ◽  
Daniel J. Cantliffe ◽  
Russell T. Nagata
Keyword(s):  
Temperature Limit ◽  
Lettuce Seed ◽  
Night Temperature ◽  
Temperature Regimes ◽  
Percent Germination ◽  
Lettuce Seed Germination ◽  
Growth Chambers ◽  
Effects Of Temperature ◽  
Dark Green ◽  
High Soil Temperature

Lettuce (Lactuca sativa L.) seeds can fail to germinate at temperatures above 24 °C. The degree of thermotolerance is thought to be at least partly related to the environment under which the seed developed. In order to study the effects of temperature during seed development on subsequent germination, various lettuce genotypes were screened for their ability to germinate at temperatures ranging from 20 to 38 °C. Seeds of the selected genotypes `Dark Green Boston' and `Valmaine' (thermosensitive), `Floricos 83', `Everglades', and PI 251245 (thermotolerant) were produced at 20/10, 25/15, 30/20, and 35/25 °C day/night temperature regimes in plant growth chambers. Seeds were germinated on a thermogradient bar from 24 to 36 °C under 12 h light/dark cycles. As germination temperature increased, the number of seeds that failed to germinate increased. Above 27 °C, seeds matured at 20/10 or 25/15 °C exhibited a lower percent germination than seeds that matured at 30/20 or 35/25 °C. Seeds of `Dark Green Boston' and `Everglades' that matured at 30/20 °C exhibited improved thermotolerance over those that matured at lower temperatures. Seeds of `Valmaine' produced at 20/10 °C exhibited 40% germination at 30 °C, but seeds that matured at higher temperatures exhibited over 95% germination. Germination of `Valmaine' at temperatures above 30 °C was not affected by seed maturation temperature. The upper temperature limit for germination of lettuce seed could thus be modified by manipulating the temperature during seed production. The potential thermotolerance of seed thereby increased, wherein thermosensitive genotypes became thermotolerant and thermotolerant genotypes (e.g., PI251245) germinated fully at 36 °C. This information is useful for improving lettuce seed germination during periods of high soil temperature, and can be used to study the biology of thermotolerance in lettuce.

scholarly journals Preventing thermoinhibition in a thermosensitive lettuce genotype by seed imbibition at low temperature

2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Temperature Dependent ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lettuce Seed Germination ◽  
Dark Green

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.

Inter- and intra-varietal variation in aerobic methane emissions from environmentally stressed pea plants

Botany ◽  
2018 ◽  
Vol 96 (12) ◽  
pp. 837-850 ◽  
Author(s):  
Awatif M. Abdulmajeed ◽  
Mohammad I. Abo Gamar ◽  
Mirwais M. Qaderi
Keyword(s):  
Stress Factors ◽  
Interactive Effects ◽  
Controlled Environment ◽  
Uvb Radiation ◽  
Pisum Sativum L ◽  
Temperature Regimes ◽  
Varietal Variation ◽  
Growth Chambers ◽  
Effects Of Temperature ◽  
Two Temperature

Environmental stress factors can influence methane (CH4) emissions from plants. There are a few studies on the interactive effects of stress factors on plant aerobic CH4, but none on the comparative evaluation of CH4 emissions between and among plant varieties. We examined the effects of temperature, UVB radiation, and watering regime on CH4 emissions from 10 pea (Pisum sativum L.) varieties first and then selected two varieties with the highest (237J Sundance; var. 1) and lowest (422 Ho Lan Dow; var. 2) emissions for further studies. Plants were grown in controlled-environment growth chambers under two temperature regimes (22 °C / 18 °C and 28 °C / 24 °C, 16 h light / 8 h dark), two UVB levels (0 and 5 kJ·m−2·d−1), and two watering regimes (well-watered and water-stressed) for 14 days, after one week of growth under 22 °C / 18 °C. Higher temperatures and water stress increased CH4 emissions, and increased emission was associated with stress. Pea varieties varied in growth and CH4 emissions; var. 1 was more stressed and had higher emission than var. 2. In the stressed variety, the water-stressed plants grown under higher temperatures at UVB5 had the highest CH4 emission, whereas the well-watered plants grown under lower temperatures at UVB5 had the lowest emission. We conclude that climatic stress conditions increase CH4 emissions, which vary with plant varieties.

Some factors affecting the development and biocontrol of cotton seedling disease

1995 ◽  
Vol 35 (6) ◽  
pp. 771 ◽  
Author(s):  
HJ Ogle ◽  
AM Stirling ◽  
PJ Dart
Keyword(s):  
Pythium Ultimum ◽  
Disease Development ◽  
Symptom Development ◽  
Night Temperature ◽  
Factors Affecting ◽  
Seedling Disease ◽  
Susceptibility To Infection ◽  
Temperature Regimes ◽  
Effects Of Temperature ◽  
Number Of Seeds

The effects of temperature and cultivar on disease development in cotton were investigated in addition to the duration of susceptibility to infection and the timing of infection by Pythium ultimum and Rhizoctonia solani. Symptom development was also monitored. Disease was more severe at day/night temperature regimes of 20/15, 25/20, and 30/25�C than at 35/30�C. Disease development differed significantly between cotton cvv. Deltapine 90 and Siokra 1-4 at 30/25�C and 35/30�C. In glasshouse trials in field soil, both R. solani and P ultimum were isolated from seeds as early as 2 h after inoculation, although most seeds were not infected with P. ultimum until 10 h after inoculation and with R. solani until 24 h after inoculation. Increasing the duration of exposure to inoculum increased the number of seeds infected and reduced the number of plants surviving. Seedlings were resistant to P. ultimum infection by 14 days after sowing but were not resistant to infection by R. solani until 28 days after sowing.

scholarly journals Ethylene evolution and endo-beta-mannanase activity during lettuce seed germination at high temperature

2004 ◽  
Vol 61 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Warley Marcos Nascimento ◽  
Daniel James Cantliffe ◽  
Donald John Huber
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Seed Priming ◽  
Inhibitory Effect ◽  
Lettuce Seed ◽  
Ethylene Evolution ◽  
Radicle Protrusion ◽  
Mannanase Activity ◽  
Lettuce Seed Germination ◽  
Dark Green

High temperatures during lettuce seed imbibition can delay or completely inhibit germination and the endosperm layer appears to restrict the radicle protrusion. The role of endo-beta-mannanase during lettuce seed germination at 35°C and the influence of ethylene in endo-beta-mannanase regulation were investigated. Seeds of 'Dark Green Boston' (DGB) and 'Everglades' (EVE) were germinated in water, or 10 mmol L-1 of 1-aminocyclopropane-1-carboxylic acid (ACC), or 10 mmol L-1 of aminoethoxyvinylglycine (AVG), or 20 mmol L-1 of silver thiosulphate (STS). Seeds were also primed in polyethylene glycol (PEG), or PEG + ACC, PEG + AVG, or PEG + STS. Untreated seeds germinated 100% at 20°C. At 35°C, EVE seeds germinated 100%, whereas DGB seeds germinated only 33%. Seed priming or adding ACC during incubation increased germination at 35°C. Higher ethylene evolution was detected in EVE than in DGB during germination at 35°C. AVG did not inhibit seed germination of DGB at 35°C, but STS did. Higher endo-beta-mannanase activity was observed in EVE compared with DGB seeds. Providing ACC either during priming or during germination increased endo-beta-mannanase activity, whereas AVG and STS led to decreased or no activity. Ethylene may overcome the inhibitory effect of high temperature in thermosensitive lettuce seeds due to increased endo-beta-mannanase, possibly leading to weakening of the endosperm.

scholarly journals Thermotolerance in Lettuce

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 777A-777
Author(s):  
Yu Sung ◽  
D.J. Cantliffe ◽  
R.T. Nagata
Keyword(s):  
Germination Percentage ◽  
Mother Plant ◽  
Night Temperature ◽  
Lettuce Seeds ◽  
Germination Temperature ◽  
Mother Plants ◽  
Plant Grown ◽  
Growth Chambers ◽  
Dark Green

Lettuce seeds differentially fail to germinate at temperatures above 21C according to genotype. Twenty-one lettuce lines were screened for their ability to germinate at temperatures from 24C to 36C. Four cultivars, `Dark Green Boston', `Valmaine', `Floricos 83', and `PI251245', were selected for this study because of their range of ability to germinate at temperatures above 24C. Seeds of the four cultivars were collected from mother plants grown in growth chambers at 20/10C(day/night temperature), 25/15C, 30/20C and 35/25C. Seeds were germinated on a thermogradient table from 24C to 36C under light (12 h). Seeds from `Floricos 83' produced above 30C had higher germination percentage at 33C and 36C than those produced below 30C temperatures. At 30C germination temperature seeds of `Valmaine' produced above 30C had 98% germination compared to 45% of those produced below 30C. `Dark Green Boston' seeds produced at 35C had higher germination percentage(70%) at 30C than those produced at other temperatures. Seeds collected from the mother plant grown above 30C day temperatures had greater germination than those grown below 30C.

scholarly journals Using a Puncture Test to Identify the Role of Seed Coverings on Thermotolerant Lettuce Seed Germination

1998 ◽  
Vol 123 (6) ◽  
pp. 1102-1106 ◽  
Author(s):  
Yu Sung ◽  
Daniel J. Cantliffe ◽  
Russell Nagata
Keyword(s):  
Seed Germination ◽  
Turgor Pressure ◽  
Seed Priming ◽  
Lettuce Seed ◽  
Tissue Resistance ◽  
Radicle Protrusion ◽  
Puncture Force ◽  
Lettuce Seed Germination ◽  
Dark Green

Temperature is an important environmental factor that affects lettuce (Lactuca sativa L.) germination. The present research was conducted to determine the role of seed coverings on lettuce seed germination at high temperature. Five lettuce genotypes were primed in order to bypass thermoinhibitional effects on germination. During germination of primed and nonprimed seeds, imbibition followed a normal triphasic pattern. Primed seeds had higher final water content, a decreased imbibitional phase II, and germinated at 36 °C compared to nonprimed seeds of thermosensitive genotypes, which did not germinate at 36 °C. Puncture tests were conducted to determine the force required to penetrate the whole seed or endosperm of the five genotypes at 24 and 33 °C. `Dark Green Boston', a thermosensitive genotype, had the highest mean resistance (0.207 N) and PI 251245, a thermotolerant genotype, had the lowest (0.139 N). Resistance to penetration of the endopserm of the five genotypes was different at both temperatures. However, three thermotolerant genotypes had lower endosperm resistance than two thermosensitive types. At 36 °C, the penetration force for primed and nonprimed seeds was compared after the first hour of imbibition and 1 hour before radicle protrusion. The force required to penetrate the seed was affected by genotype, seed priming, and duration of imbibition. Puncture force decreased as imbibition time at 36 °C increased in primed and nonprimed seed of each thermotolerant genotype but not in the thermosensitive genotypes. Priming reduced the initial force necessary to penetrate the seed and endosperm in all genotypes. Thus, for radicle protrusion to occur, there must first be a decrease in the resistance of the endosperm layer as evidenced by priming or thermotolerant genotype. Then, the pericarp and integument are sufficiently weakened so that tissue resistance is lower than the turgor pressure of the expanding embryo, allowing germination to be completed.

scholarly journals Edamame Production as Influenced by Seedling Emergence and Plant Population

2005 ◽  
Vol 15 (3) ◽  
pp. 672-676 ◽  
Author(s):  
Elsa Sánchez ◽  
Kathleen Kelley ◽  
Lynn Butler
Keyword(s):  
Glycine Max ◽  
Seedling Emergence ◽  
Commercial Production ◽  
Effect Of Temperature ◽  
Night Temperature ◽  
Plant Populations ◽  
Major Constraint ◽  
Temperature Regimes ◽  
Central Pennsylvania ◽  
Growth Chambers

Eight edamame (Glycine max) cultivars were evaluated in the field in 2002, 2003, and 2004 to determine suitability for growing in central Pennsylvania. Data collection included plant populations (percent stand), marketable and unmarketable yields and edamame pod and bean quality indicators. Plant populations varied by year and cultivar and were generally below 80%. The effect of temperature on seedling emergence, and therefore plant populations, was evaluated for four edamame cultivars by using growth chambers programmed with varying day/night temperature regimes. Seedling emergence varied by cultivar and was generally below 80% with two exceptions. When grown in a 70/60 °F day/night temperature regime, `Butterbeans', and `Early Hakucho' exceeded 80% seedling emergence. In the field trial, plant populations affected marketable yields. Pod and bean quality were dependent on cultivar. Results indicated that `Butterbeans', `Early Hakucho', `Green Legend', `Shironomai', `Butterbaby', and `Lucky Lion' appear promising for growing in Pennsylvania based on pod and bean quality. However, the issue of poor seedling emergence and plant populations presents a major constraint to commercial production and needs to be studied further.

scholarly journals EFFECTS OF TEMPERATURE ON PHOTOSYNTHATE PARTITIONING IN ALFALFA

1990 ◽  
Vol 70 (1) ◽  
pp. 203-208 ◽  
Author(s):  
SAFAA AL-HAMDANI ◽  
GLENN W. TODD
Keyword(s):  
Medicago Sativa ◽  
Foliar Application ◽  
Specific Activity ◽  
Photosynthate Partitioning ◽  
Plant Parts ◽  
Temperature Regimes ◽  
Total Plant ◽  
Medicago Sativa L ◽  
Growth Chambers ◽  
Effects Of Temperature

This study was conducted to examine the effects of three temperature regimes (12/2 °C, 21/8 °C, and 34/25 °C day/night) on photosynthate partitioning in alfalfa (Medicago sativa L.). Plants were grown under greenhouse conditions for approximately 3 mo and then clipped back. When regrowth was at the bud stage, the plants were placed in growth chambers at the three temperatures, all having a photoperiod of 14 h. Two hours after the onset of the photoperiod of the second day, foliar application of 14C-labelled urea was used to provide a source of 14CO2 for tracing the movement of photosynthate from the source leaf to other parts of the alfalfa plant. Plants were harvested 24 h later and each plant was subdivided into six portions. The plant parts included the source leaf, treated upper shoot, treated lower shoot, untreated shoot, crown and roots. The percent of 14C exported of the total recovered radioactivity by the source leaf increased as temperature increased. As temperature increased, radioactivity recovery was increased in treated upper shoot, untreated shoots, and roots. The highest recovery of 14C by the crown was at 21/8 °C. followed by those in 12/2 °C and 34/25 °C, respectively. Total 14C recovered from the treated lower shoot was decreased as temperature increased.Key words: Relative specific activity (RSA), total plant radioactivity, translocation, 14C, Medicago sativa L., alfalfa

scholarly journals Substrate Acidification by Geranium: Temperature Effects

2008 ◽  
Vol 133 (4) ◽  
pp. 508-514 ◽  
Author(s):  
Matthew D. Taylor ◽  
Paul V. Nelson ◽  
Jonathan M. Frantz
Keyword(s):  
High Temperature ◽  
Dry Weight ◽  
Night Temperature ◽  
The Third ◽  
Temperature Regimes ◽  
Higher Temperature ◽  
Effects Of Temperature ◽  
Increasing Temperature ◽  
Substrate Ph ◽  
Tissue Phosphorus

Sudden pH decline (SPD) describes the situation where crops growing at an appropriate pH rapidly (within 1–2 weeks) cause the substrate pH to shift downward one to two units. ‘Designer Dark Red’ geraniums (Pelargonium ×hortorum Bailey) were grown in three experiments to assess possible effects of temperature on SPD. The first experiment tested the effect of four day/night temperature regimes (14 °C day/10 °C night, 18 °C day/14 °C night, 22 °C day/18 °C night, and 26 °C day/22 °C night) on substrate acidification. At 63 days after transplanting (DAT), substrate pH declined from 6.8 to 4.6 as temperature increased. Tissue phosphorus (P) of plants grown at the highest three temperatures was extremely low (0.10%–0.14% of dry weight), and P stress has been reported to cause acidification. It was not possible to determine if the drop in substrate pH was a singular temperature effect or a combination of high temperature and low P. To resolve this, a second experiment tested a factorial combination of the three highest temperatures from the first experiment and five preplant P rates (0, 0.065, 0.13, 0.26, or 0.52 g·L−1 substrate). Regardless of tissue P concentrations, which ranged from deficient to above adequate, substrate pH decreased with increasing temperature. At 63 DAT, in the 0.065 and 0.13 P treatments, tissue P was deficient and pH decreased with increasing temperature from 5.6 to 4.7 and 5.9 to 4.7, respectively. In the 0.26 P treatment, tissue P was adequate at the lowest temperature and there was no acidification. At the mid- and highest temperatures, tissue P was deficient and statistically equivalent, yet pH decreased to 5.2 and 4.7, respectively. In the highest P treatment, tissue P levels were unaffected by temperature, above adequate, and pH declined with each increase in temperature from 6.5 to 5.0. The results at 63 DAT once more showed that temperature acted independent of tissue P and caused geraniums to acidify the substrate. In the third experiment, the amount of acidity produced by roots of plants grown at the two highest temperatures used in the first two experiments was quantified. Plants grown at the higher temperature produced 28% more acid per gram dry root. The results herein indicate that high temperature can induce SPD by geranium.

scholarly journals Physiology of Sugar-Cane VII. Effects of Temperature, Photoperiod Duration, and Diurnal and Seasonal Temperature Changes on Growth and Ripening

1965 ◽  
Vol 18 (1) ◽  
pp. 53 ◽  
Author(s):  
KT Glasziou ◽  
TA Bull ◽  
MD Hatch ◽  
PC Whiteman
Keyword(s):  
Sugar Cane ◽  
Temperature Effects ◽  
Interaction Effects ◽  
Seasonal Temperature ◽  
Night Temperature ◽  
Temperature Changes ◽  
Temperature Regimes ◽  
Controlled Environments ◽  
Effects Of Temperature ◽  
Period Duration

Independent and interaction effects of day and night temperature, photo-period duration, and diurnal thermoperiodicity were studied on sugar-cane grown under controlled environments. During the first 3 months of growth, day and night temperature effects were mainly additive, but at 6 months the interaction effects of all variables were numerous and complex. Many of the interaction effects could be attributed to increased responses to constant-temperature regimes with a 12-hr photoperiod. No evidence for thermoperiodicity requirements was found.

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