scholarly journals Genotypic and Environmental Variation in Antioxidant Activity and Total Phenolic Content among Blackberry and Hybridberry Cultivars

2005 ◽  
Vol 130 (4) ◽  
pp. 527-533 ◽  
Author(s):  
Ann Marie Connor ◽  
Chad E. Finn ◽  
Peter A. Alspach
Keyword(s):  
Antioxidant Activity ◽  
Total Variation ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Environmental Variation ◽  
Fruit Weight ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Environment Interaction ◽  
Trait Variance

Antioxidant compounds absorbed from our diet are thought to have a role in preventing chronic diseases that result from oxidative damage. Berry fruit have high levels of antioxidants, and further increases in antioxidant activity (AA) might be possible through breeding. We determined the AA, total phenolic content (TPH), and fruit weight in 16 blackberry and hybridberry (Rubus L.) cultivars harvested in New Zealand and Oregon in 2002 and 2003, to assess genetic and environmental variation. Both AA and TPH varied significantly between years within location, but not among cultivars or between locations per se. However, cultivar interactions with both location and year within location contributed to variation in both variates. In contrast, both cultivar and location contributed to variation in fruit weight, but years within location did not. However, the cultivar × year within location interaction was significant for this trait. Variance component distributions confirmed that cultivar and location effects together contributed little (<20%) to the total variation in either AA or TPH, while cultivar × environment interactions accounted for >50% of total variation in these traits. Cultivar and location effects together contributed ≈70% of the total variation observed in fruit weight. Phenotypic correlations were significant between AA and fruit weight (r = -0.44), and between TPH and fruit weight (r = -0.51). When adjusted for fruit weight, analyses for AA and TPH demonstrated that cultivar effects approached significance (P = 0.06) and accounted for ≈25% of total variance, while location effects accounted for none. Although the cultivars in this study had diverse interspecific backgrounds, utilization of various Rubus species in blackberry and hybridberry breeding is not uncommon, and our results demonstrating significant cultivar × environment interaction for AA and TPH should be applicable to breeding for high AA genotypes.

scholarly journals Variation and Heritabilities of Antioxidant Activity and Total Phenolic Content Estimated from a Red Raspberry Factorial Experiment

2005 ◽  
Vol 130 (3) ◽  
pp. 403-411 ◽  
Author(s):  
Ann Marie Connor ◽  
M. Joseph Stephens ◽  
Harvey K. Hall ◽  
Peter A. Alspach
Keyword(s):  
Antioxidant Activity ◽  
Total Variation ◽  
Total Phenolic Content ◽  
Variance Components ◽  
Phenolic Content ◽  
Fruit Weight ◽  
Total Phenolic ◽  
Narrow Sense ◽  
Red Raspberry ◽  
Sib Families

Variance components and narrow-sense heritabilities were estimated for antioxidant activity (AA), total phenolic content (TPH), and fruit weight in red raspberry (Rubus idaeus L.) fruit from offspring of a factorial mating design. Forty-two full-sib families utilizing seven female and six male parents were evaluated in each of two years in Motueka, New Zealand. In a single year, values within individual half-sib families ranged as widely as 25.3-79.4 μg·g-1 fruit for AA, 205-597 mg/100 g fruit for TPH, and 1.06-7.69 g for fruit weight. Analyses of variance for these three variates demonstrated significant parental source variation in both individual and combined year analyses. For AA and TPH, female parental effects accounted for ≈7% to 19% of total variation, while male effects accounted for ≈6% to 8%. A partially pigment deficient R. parvifolius L. derivative female parent accounted for some of these differences. Female × male parent interaction was not significant for AA and TPH and was marginally significant for fruit weight in combined year analysis. Year had a significant effect on the overall mean AA and TPH, but contributed less than genetic effects to the overall variation in all three traits. Interactions of year with genetic effects were not statistically significant for AA or TPH, indicating that between-year rank or scale changes among families were negligible. The largest proportion of variation was found within rather than among full-sib families. However, variation among plots within full-sib families accounted for 12% to 19% of total variation, indicating environmental differences accounted for some of the observed within-family variation in AA and TPH. Antioxidant activity and TPH were highly phenotypically correlated (r = 0.93); their genetic correlation (r = 0.59) implies that substantial additive genetic factors underlie the phenotypic correlation, but that nonadditive genetic or environmental influences are also important. Both AA and TPH were weakly negatively phenotypically correlated with fruit weight (r = -0.34 and -0.33, respectively), but the corresponding genetic correlations were close to zero. Thus, selection for both high AA or TPH and high fruit weight is possible. Narrow-sense heritability estimates based on variance components from combined year data were h2 = 0.54, 0.48, and 0.77 for AA, TPH, and fruit weight, respectively. These estimates imply a rapid response to selection is possible.

scholarly journals Investigation on Antioxidant Compounds from Marine Algae Extracts Collected from Binuangeun Coast, Banten, Indonesia

2017 ◽  
Vol 11 (2) ◽  
pp. 59 ◽  
Author(s):  
Muhammad Nursid ◽  
Endar Marasskuranto ◽  
Karren Budi Atmojo ◽  
Mulyo Pandu Hartono ◽  
Maria Diyah Nur Meinita ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Total Phenolic Content ◽  
Marine Algae ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Antioxidant Compound

Marine algae contains bioactive secondary metabolites that  have potential to be developed as antioxidant. The aims of  this research were to investigate antioxidant activity and total phenolic compound of marine algae collected from Binuangeun Beach, Banten and to characterize antioxidant compounds from selected algae species. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and Ferric Ion Reducing Antioxidant Power (FRAP) Assay, while total phenolic content was estimated by Folin-Ciocalteau method. Isolation of bioactive antioxidant compound was performed using SiO2 column chromatography and preparative high performance liquid chromatography (HPLC). The antioxidan activity evaluation was conducted to methanol extracts  from 20 species (7 Chlorophyta, 9 Phaeophyta and 4 Rhodophyta). Results showed that Padina australis extract was found to be the most active. The major bioactive antioxidant compound was identified as fucoxanthin and a polar compound that was suspected as phenolic compound. The extracts of P. australis presented the highest phenolic content (58.59 mg GAE/g). A significant correlation between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of P. australis.

scholarly journals Effects of ingredients and cooking time on total phenolic content and antioxidant activity of different homemade tomato sauces

2021 ◽  
Vol 7 (2) ◽  
pp. 84-90
Author(s):  
Çağla Özer
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Cooking Time ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Tomato Sauce ◽  
Tomato Product ◽  
Lipophilic Antioxidant ◽  
Culinary Culture

Tomato is one of the most consumed fruits in the world. Tomato sauce, a tomato product, is one of the mother sauces that has been developed by Escoffier (1846-1935). Nowadays, tomato sauce has been adapted by each country according to their own culinary culture. In the current study four different tomato sauce recipes were investigated in the context of their antioxidant activity and total phenolic content. Hydrophilic and lipophilic extractions of samples were evaluated separately. It was found that there is a relationship between total phenolic content and antioxidant activity in hydrophilic extraction. However, no similar correlation was found between total phenolic content and antioxidant activity in lipophilic extraction. According to the results, the highest antioxidant activity of both hydrophilic and lipophilic extractions was observed in S1 with the values 25.53±1.01 and 45.48±2.06 respectively. In lipophilic extraction, S2 had the lowest antioxidant activity with 32.77±1.07, because of the lack of the ingredients high in lipophilic antioxidant compounds. It was observed that the shortest cooking time with 10 min provide the highest retention of antioxidant activity and total phenolic content in this sauce. Also, antioxidant activity of lipophilic extraction for longer cooking time applied sauces (S4) could be kept high with ingredients such as carrot. It can be suggested that adding of ingredients which have antioxidant activity may be useful for providing bioactive properties in long cooking time procedures and it should be noted that the length of cooking time negatively affected the TPC values.

scholarly journals Broa, an Ethnic Maize Bread, as a Source of Phenolic Compounds

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 672
Author(s):  
Andreia Bento-Silva ◽  
Noélia Duarte ◽  
Elsa Mecha ◽  
Maria Belo ◽  
Ana Teresa Serra ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Hydroxycinnamic Acids ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Maize Varieties ◽  
Hydroxycinnamic Acid Amides ◽  
Traditional Processing

Maize is an important source of phenolic compounds, specially hydroxycinnamic acids, which are widely known for their antioxidant activity and associated health benefits. However, these effects depend on their bioaccessibility, which is influenced by the different techniques used for food processing. Several traditional products can be obtained from maize and, in Portugal, it is used for the production of an ethnic bread called broa. In order to evaluate the effect of processing on maize phenolic composition, one commercial hybrid and five open-pollinated maize flours and broas were studied. The total phenolic content and antioxidant activity were evaluated by the Folin-Ciocalteu and ORAC assays, respectively. The major phenolics, namely ferulic and p-coumaric acids (in their soluble-free, soluble-conjugated and insoluble forms), insoluble ferulic acid dimers and soluble hydroxycinnamic acid amides were quantitated. Results show that the total phenolic content, antioxidant activity and hydroxycinnamic acids resisted traditional processing conditions used in the production of broas. The content in soluble-free phenolics increased after processing, meaning that their bioaccessibility improved. Portuguese traditional broas, produced with open-pollinated maize varieties, can be considered an interesting dietary source of antioxidant compounds due to the higher content in hydroxycinnamic acids and derivatives.

scholarly journals Genotypic and Environmental Variation in Antioxidant Activity, Total Phenolic Content, and Anthocyanin Content among Blueberry Cultivars

2002 ◽  
Vol 127 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Ann Marie Connor ◽  
James J. Luby ◽  
Cindy B.S. Tong ◽  
Chad E. Finn ◽  
James F. Hancock
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Free Radical Oxidation ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Environment Interaction ◽  
Total Anthocyanin ◽  
Radical Oxidation ◽  
Single Location

Dietary antioxidants may have a role in preventing some of the chronic diseases in humans resulting from free radical oxidation of lipids and other cellular components. Blueberries (Vaccinium L. sp.) are considered one of the best fresh fruit sources of antioxidants, and there is the potential to increase the antioxidant activity further through breeding. Thus, the variability of fruit antioxidant activity (AA) was examined among a set of 16 highbush and interspecific hybrid cultivars grown at locations in Minnesota (MN), Michigan (MI), and Oregon (OR) over 2 years (1998 and 1999) to determine effects of genotype, year, and location. Nine cultivars were common to all three locations in both years. Antioxidant activity, total phenolic content (TPH), and total anthocyanin content (ACY), were determined in triplicate samples from each genotype. Cultivars differed significantly (P ≤ 0.05) in AA, TPH, and ACY both within and over locations. The single location mean AA for all cultivars changed significantly between the 2 years in OR and in MI, while the single location mean for TPH differed between the 2 years in MN and MI. Changes in cultivar rank were significant for AA, TPH, and ACY between years within each location. Significant changes in rank for TPH and ACY were also noted between pairs of locations as well. Pearson's correlation for AA (based on cultivar means) appeared highest between MN and OR (r = 0.90) and MN and MI (r = 0.69) in 1998; correlations between locations for the combined years were 0.74 for MN and OR, 0.55 for MN and MI and 0.45 for MI and OR. For the group of nine cultivars, AA correlated well with TPH within each location, with r ranging from 0.67 to 0.95 for data from individual and combined years. Correlation of AA with ACY at each location was lower than that for AA with TPH, in both individual and combined years. This study demonstrates significant genotype× environment interaction for AA in blueberry.

scholarly journals Extraction Process Optimization, Identification, and Profiling of Phenolic Antioxidant Compounds from the Fruits of Ficus auriculata

2020 ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Process ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Experimental Findings ◽  
Optimum Extraction

Abstract In this study, the extraction conditions to maximize the antioxidant activity and total phenolic content of Ficus auriculata were optimized using response surface methodology. For the purpose of extraction, the Ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P<0.0001) and total phenolic content (R2 = 0.961, P<0.0001), indicating a significant correlation between the experimental and expected value. The highest antioxidant activity (85.20 ± 0.96 %) for DPPH were achieved at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC-ESI-MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals Evaluation of Antioxidant Activity, Toxicity, and Phenolic Profile of Aqueous Extracts of Chamomile (Matricaria chamomilla L.) and Sage (Salvia officinalis L.) Prepared at Different Temperatures

2020 ◽  
Vol 10 (7) ◽  
pp. 2270 ◽  
Author(s):  
Nefeli S. Sotiropoulou ◽  
Stiliani F. Megremi ◽  
Petros Tarantilis
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Vibrio Fischeri ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Aqueous Extracts ◽  
Different Temperatures ◽  
Phenolic Profiles

Chamomile and sage are common herbs that are mostly used as infusions due to their beneficial properties. The aims of this study were to determine the total phenolic content, antioxidant activity, and potential toxicity of chamomile and sage aqueous extracts prepared at three different temperatures (25, 80, 100 °C) and finally, to detect their phenolic profiles at the optimum temperature. In order to measure the total phenolic content and antioxidant capacity, Folin–Ciocalteu and 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) assays were applied, respectively. The extraction temperature at 80 °C was the optimum, with maximal antioxidant activity and the highest total phenolic content for both herbs. Luminescence-based assay demonstrated that all the examined aqueous extracts possessed toxicity towards Vibrio fischeri. Microtox assay demonstrated no correlation with the other two assays, which were positively correlated. The major phenolics of chamomile were rutin trihydrate, ferulic acid, chlorogenic acid, and apigenin-7-O-glucoside; and major phenolics of sage were rosmarinic acid, salvianolic acid K, and luteolin-7-O-glucuronide, as defined by LC-MS of aqueous extracts at 80 °C. It can be concluded that the extraction of herbal aqueous extracts at 80 °C can provide significant bioactive and antioxidant compounds, but their consumption must be in moderation.

LC-DAD-ESI-MS/MS, total phenolic content, and antioxidant activity of Firmiana simplex

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
MA Ghareeb ◽  
T Mohamed ◽  
AM Saad ◽  
LA Refahy ◽  
MA Sobeh ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Esi Ms
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