Determination of the role of polymorphic variants of the interferon-gamma gene (IFNG) in the development of tuberculosis in the Yakut population

The objective of the study: to determine the role of polymorphic variants T6000A (rs2430561), T8360C (rs2069718) of the IFNG gene in the development of tuberculosis in the population of Yakut nationality.Subjects and methods. The case-control study was conducted. The subject for the study was DNA samples from 172 tuberculosis patients (the case group) and 367 people with an excluded diagnosis of tuberculosis (the control group). All participants of the study belonged to Yakut nationality. For genotyping, DNA was isolated from venous blood. The genotyping was performed by real-time polymerase chain reaction. For statistical processing, Pearson's χ2 test and odds ratio with 95% confidence intervals were used.The results of the study showed that the distribution of genotypes of polymorphisms T6000A (rs2430561), T8360C (rs2069718) of the IFNG gene in the case and control groups corresponded to the Hardy – Weinberg equilibrium. There was no statistically significant difference in frequencies of alleles and genotypes of polymorphisms T6000A (rs2430561), T8360C (rs2069718) of the IFNG gene in the patients from the case and control groups.Conclusions. The results of the study did not reveal the association of alleles and genotypes of the rs2430561 and rs2069718 polymorphic variants of the IFNG gene with the risk of developing tuberculosis for people of Yakut nationality.

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Variants in the 3′ End of SLC6A3 in Northwest Han Population with Parkinson’s

Parkinson s Disease ◽

10.1155/2019/6452471 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Peiye Chang ◽

Yongwang Fu ◽

Ping Zhao ◽

Chunmei Wang ◽

Mingfang Jiang ◽

...

Keyword(s):

Genetic Factors ◽

Allele Frequencies ◽

Control Group ◽

Control Groups ◽

Han Population ◽

Significant Difference ◽

And Control ◽

Normal Controls ◽

Genotype And Allele Frequencies

Parkinson’s disease (PD) is one of the most common neurodegenerative disorders in neurology. It is possible that multifactorial and genetic factors are related to its pathogenesis. Recently, there have been reports of SLC6A3 genetic variants leading to PD. However, the role of 3′ end of SLC6A3 in PD is less studied in different ethnic groups. To explore the roles of 3′ end of SLC6A3 in PD development, 17 SNP sites in 3′ end of SLC6A3 were analyzed in 360 PD patients and 392 normal controls of Han population residing in northwest of China. The significant difference of gene type and allele frequencies between the PD and control groups was detected only in rs40184 (P = 0.013 and 0.004, respectively; odds ratio 2.529, 95% confidence interval 1.325–4.827). The genotype and allele frequencies of the other 16 SNP sites were not found to be different between the PD group and the control group. rs2550936, rs3776510, and rs429699 were selected to construct the haplotypes; no significant difference was found in a frequency of 5 haplotypes between the PD group and the control group. These results suggest that the SLC6A3 variant in rs40184 A allele may increase the risk of PD in northwest Han population and may be a biomarker of PD.

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Effects of Tranexamic Acid on Mortality and Blood Transfusion in Trauma Patients with Significant Hemorrhage: A Clinical Trial

Advances in Bioscience and Clinical Medicine ◽

10.7575/aiac.abcmed.17.05.04.04 ◽

2017 ◽

Vol 5 (4) ◽

pp. 24

Author(s):

Farzad Kakaei ◽

Peyman Virani ◽

Shahriar Hashemzadeh ◽

Sina Zarrintan ◽

Samad Beheshtirouy ◽

...

Keyword(s):

Blood Transfusion ◽

Tranexamic Acid ◽

Study Groups ◽

Control Group ◽

Case Group ◽

Trauma Patients ◽

Transfusion Rate ◽

Control Groups ◽

Significant Difference ◽

And Control

Extensive hemorrhage is a significant cause of mortality in trauma patients. Tranexamic acid has been used for controlling bleeding in cardiovascular surgeries and dental manipulations in patients with hemophilia. However, in traumatic patients with bleeding, its use dates back to more recent years. This study aims to examine the effects of this drug on reducing mortality and blood transfusion rate in trauma patients with significant hemorrhage. A total of 60 patients with significant trauma-related hemorrhage (systolic blood pressure < 90 mmHg/heart rate > 110/min) from the emergency department of Imam Reza Hospital (Tabriz, Iran), were randomized in two groups. The case group received intravenous Tranexamic acid (1 g in 10 min and then 1 g over 8 h). The control group received placebo. Rate of transfusion and rate of one-month mortality were compared between the study groups. The mean ICU stay and overall hospitalization times did not have significant difference between two groups (p<0.05). Transfusion of packed cells was 6.03±1.50 and 6.03±1.22 units in case and control groups respectively. Transfusion of fresh frozen plasma (FFP) was 2.50±1.36 and 3.03±0.96 units in case and control groups respectively (p=0.09). Transfusion of platelets was 0.40±0.20 1.33±0.31 units in case and control groups respectively (p=0.01). Three patients (10%) in the case group and 4 patients (13.3%) in the control group were expired (p=0.50). Tranexamic acid is safe and effective in reducing platelet transfusion rate in patients with trauma-related significant hemorrhage. However, transfusion need and mortality would not reduce by its use in trauma patients.

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Association among Thyroid Dysfunction, Asthma, Allergic Rhinitis and Eczema in Children with Alopecia Areata

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.050 ◽

2017 ◽

Vol 5 (3) ◽

pp. 305-309 ◽

Cited By ~ 1

Author(s):

Javad Ghaffari ◽

Ghasem Rahmatpour Rokni ◽

Armaghan Kazeminejad ◽

Hosein Abedi

Keyword(s):

Allergic Rhinitis ◽

Atopic Dermatitis ◽

Alopecia Areata ◽

Allergic Diseases ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Significant Difference ◽

History Of ◽

And Control

BACKGROUND: Alopecia areata is a non-scarring hair loss, which typically starts quickly. Atopy is one of the possible predisposing risk factors for this condition.AIM: This study aimed to evaluate the prevalence of thyroid disease, atopic dermatitis and allergic diseases in children with alopecia areata and compare the results with healthy individuals.METHODS: This case-control study was conducted on 50 patients with alopecia areata, diagnosed by a dermatologist, and 150 healthy individuals as the control group. Participants filled the questionnaires, and necessary tests were performed.RESULTS: In this study, the mean age of the participants was 2.55 Â± 14.26 and 3.19 Â± 11.92 in the case and control groups, respectively. Prevalence of asthma was 22% in the case group and 12.5% in control group (P = 0.109). Also, allergic rhinitis and eczema were observed in 20% and 22% of the subjects of the case group, whereas they were reported to be 8% and 10% in the control group (PV = 0.03 and 0.175, respectively). Moreover, 28% and 8% of the participants in the case and control groups had a family history of atopy and allergic disorders, respectively (P = 0.046). A significant difference was observed between the two groups regarding gender, type of delivery and contact with animals.CONCLUSIONS: According to the results of this study, a significant association was observed between the prevalence of alopecia areata and atopic conditions, such as allergic rhinitis and history of atopic dermatitis.

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Genetic polymorphisms affecting antioxidant enzymes are present in tympanosclerosis patients

The Journal of Laryngology & Otology ◽

10.1017/s0022215116008732 ◽

2016 ◽

Vol 130 (10) ◽

pp. 928-933 ◽

Cited By ~ 1

Author(s):

A Akyigit ◽

Ş Yalcin ◽

E Ö Etem ◽

İ Kaygusuz ◽

T Karlidag ◽

...

Keyword(s):

Genetic Polymorphisms ◽

Otitis Media ◽

Venous Blood ◽

Chronic Otitis Media ◽

Study Groups ◽

Control Group ◽

Genotype Distribution ◽

Control Groups ◽

Significant Difference ◽

And Control

AbstractBackground:This study investigated genetic polymorphisms affecting the inducible nitric oxide synthase, superoxide dismutase and catalase enzymes in chronic otitis media patients with and without tympanosclerosis, and the role of genetic susceptibility in the disease aetiology.Methods:A total of 162 patients who underwent surgery for chronic otitis media were divided into two study groups: a tympanosclerosis group and a chronic otitis media group. A third, the control, group comprised 188 healthy volunteers. Venous blood samples were evaluated using reverse transcriptase polymerase chain reaction.Results:There was a significant difference in GG genotype distribution of the −277A>G polymorphism in the NOS2 gene between the tympanosclerosis and control groups (p < 0.05). However, there were no significant between-group differences in the CC genotype distribution of the p.Ala16Val (C>T) polymorphism in the SOD2 gene (p > 0.05). There were significant differences in the TT genotype distribution of the −21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups (p < 0.05).Conclusion:These results suggest that genetic predisposition may play a role in the aetiopathogenesis of tympanosclerosis.

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Effect of Herbal Echinacea on Recurrent Minor Oral Aphthous Ulcer

The Open Dentistry Journal ◽

10.2174/1874210601812010567 ◽

2018 ◽

Vol 12 (1) ◽

pp. 567-571 ◽

Cited By ~ 1

Author(s):

Faezeh Khozeimeh ◽

Zahra Saberi ◽

Atefeh Tavangar ◽

Fahime Fakhari Badi

Keyword(s):

Herbal Medicine ◽

Recurrence Rate ◽

Aphthous Ulcer ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Positive Effects ◽

Aphthous Ulcers ◽

Significant Difference ◽

And Control

Background: The oral aphthous is a common oral ulcer with intense pain and there is no treatment for it, yet. Echinacea is an herbal medicine that moderated the immune system. Objective: The aim of this study was to investigate the effects of Echinacea on the treatment of aphthous ulcer. Methods: 50 patients with minor aphthous participated in our study. 25 patients take 3 tablets in a day for five weeks (case group) and 25 patients didn’t take any tablets (control group). The patients were monitored for one month before taking the tablets for six months. During this period, the number of lesions, complete improvement of ulcers, recurrence rate and intensity of pain were considered in each month. Finally, the Friedman and ANOVA tests used to analyze the obtained data. Result: Our study showed a significant difference between a number of lesions during six-month in case and control groups (p>0.001). In this way, we observed that the number of lesions was decreased significantly after six months in the case group. Hence, ANOVA analysis showed a significant decrease between each month for the intensity of pain (p=0.025), complete improvement (p<0.001) and recurrence rate (p=0.026). Conclusion: In conclusion, we showed that Echinacea tablets as an herbal medicine have positive effects on a number of lesions, intensity of pain, complete improvement and recurrence rate in patients with recurrent minor aphthous ulcers.

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Studying the Frequency of Autoimmune Thyroid Diseases in Duhok Province

Science Journal of University of Zakho ◽

10.25271/sjuoz.2019.7.2.587 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Rozheen I. Hasan ◽

Amir H. Raziq

Keyword(s):

Venous Blood ◽

Serum Levels ◽

Control Group ◽

Thyroid Disorder ◽

Control Groups ◽

Autoimmune Thyroid Diseases ◽

Autoimmune Thyroid ◽

Significant Difference ◽

The One ◽

And Control

Thyroid disorders are one of the most frequent disorders affecting endocrine system. The present study aimed to identify the frequency of thyroid disorder in our locality at Duhok city. To do so, a total of one hundred patients and one hundred apparently healthy control individuals were enrolled in this study. Samples of venous blood (5 ml) were withdrawn from patients and control subjects and analysed. Clinical assessment was performed by a specialist and each patient provided with a document supporting preliminary clinical diagnosis. Measurements of serum TSH, T3, T4, and anti-thyroperoxidase antibodies (anti-TPO antibodies) serum levels were performed. All assays were conducted at Duhok central health laboratory. Out of the one hundred patients and the one hundred control groups, forty-two and thirty candidates were randomly selected, respectively. The mean TSH serum levels of 25.87 (uIU/ml) for the patients significantly differ from that of the control group 3.55 (uIU/ml) (p<0.001). However, T3 and T4 serum levels indicated no significant difference between the two categories. Moreover, there was statisticaly significant difference (p< 0.0001) between the patients and control groups when their mean serum anti-TPO concentrations were compared. In conclusion, considerable number of subjects appeared to be candidates for deranged thyroid functions and is susceptible to autoimmune thyroid disorder.

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Vitamin D3 levels in allergic rhinitis: a case control study from South Karnataka

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20202784 ◽

2020 ◽

Vol 6 (7) ◽

pp. 1295

Author(s):

Vinay S. Bhat ◽

Anupama A.

Keyword(s):

Allergic Rhinitis ◽

Vitamin D3 ◽

Serum Vitamin ◽

Normal Subjects ◽

Control Group ◽

Case Group ◽

Significant Difference ◽

Region Of Residence ◽

And Control

Background: Allergic rhinitis (AR) is a common disorder seen in routine otolaryngology practice. There has been an increased interest in researchers worldwide regarding role of vitamin D3 in pathogenesis of allergy. This study intends to compare levels of vitamin D3 in patients with clinically diagnosed allergic rhinitis and subjects without allergic rhinitis. This study was conducted in rural South Karnataka where incidence of allergic rhinitis is on the rise. Main objective of this study was to assess vitamin D3 levels in patients with clinically diagnosed AR and compare it with vitamin D3 levels in control group where normal subjects were included.Methods: Total of 100 patients were included in this study. Case group comprised of 50 patients clinically diagnosed as AR and control group comprised 50 subjects without any symptoms of AR. All the patients underwent serum vitamin D3 estimation. Other parameters such as age, gender, occupation (outdoor vs indoor) and region of residence (urban vs rural) were also compared between the groups. Results: This study did not find significant difference between two groups when all the parameters compared. Significant number of study subjects both in case and control group were found to be either deficient or inadequate in vitamin D3 levels.Conclusions: we did not find significant difference in vitamin D3 levels between cases and controls. Further studies with bigger sample size and robust study design may throw more light on association of vitamin D3 with AR.

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Evaluation of Bowel Habits in Patients with Acne Vulgaris

10.22541/au.161248618.84591551/v1 ◽

2021 ◽

Author(s):

Munise Daye ◽

Fatma Cihan ◽

Begüm Işık ◽

Berna Hafızoğlu

Keyword(s):

Patient Group ◽

Short Form ◽

Acne Vulgaris ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Severe Acne ◽

Bowel Habits ◽

Significant Difference ◽

And Control

Purpose: To evaluate the bowel habits of patients with acne vulgaris Method: In this case control study, socio-demographic characteristics of the participants (age, gender, marital status, educational status, profession, economic status, smoking-alcohol habits, chronic diseases, previous operations, people they live with, diet) were questioned and the global acne grading scores of the patients were calculated. Bristol Stool Scale, Rome III Criteria for IBS, International Physical Activity Questionnaire-Short Form, Hospital Anxiety Depression and KADF (dietary fiber information) scales fulfilled. Results: The patient group consisted 102 and the control group consisted 104 participants. The mean age was 20.9 ± 3.9 years old in the case group and 21.8 ± 5.0 years old in the control group. Patient and control groups were similar in terms of age and gender. Smoking rates were significantly higher in the patient group (p=0,035). The amount of coffee consumed per day were significantly higher in the patient group (p=0,040). According to the global acne grading scores, 55.9% (n = 57) had medium, 39.2% (n = 40) had mild, 3.9% (n = 4) had severe and 1% (n=1) had very severe acne. Anxiety scores were found to be significantly higher in the patient group (p = 0.005). When the case and control groups were compared for IBS presence, no significant difference was found (p = 0.317). Also, IBS was not related with acne severity (p = 0.162). Conclusion: Further large sample sized studies are needed on this subject, as there is strong evidence about brain–gut–skin axis existence.

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The association between serum betahuman Chorionic gonadotropin and Preeclampsia

Journal of Dhaka Medical College ◽

10.3329/jdmc.v23i1.22701 ◽

2015 ◽

Vol 23 (1) ◽

pp. 89-93 ◽

Cited By ~ 3

Author(s):

Zinat Begum ◽

Iffat Ara ◽

Shaorin Tanira ◽

Kashfia Ahmed Keya

Keyword(s):

Pregnant Women ◽

Severe Preeclampsia ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Medical College ◽

Significant Difference ◽

Blood Pressures ◽

The Mean ◽

And Control

Background: Exact aetiology of this potentially fatal disorder remains poorly understood. A number of theories have been put forward where different biochemical markers have been implicated in the causal association of preeclampsia. This study was intended to find the association between serum b-hCG level and preclampsia Methods: This cross-sectional, case-control study was conducted on 74 pregnant women with preeclampsia (cases) who were admitted in the Eclampsia ward of Dhaka Medical College Hospital, Dhaka between January and July of 2013. A total of 76 normotensive pregnant women were also taken from the Obstetrics & Gynaecology Out-patient Department of the same hospital as control. The study subjects were selected on the basis of predefined eligibility criteria. The serum levels of b-hCG were compared between case and control groups as well as between mild and severe preeclampsia. Result: The case and control groups were almost similar in terms of all the baseline demographic and obstetric characteristics except past history of PET which was significantly higher in the former group than that in the latter group. Majority (97.1%) of the cases had severe hypertension (74.3%) with mean systolic and diastolic blood pressures being 162.6 and 110.8 mmHg respectively. The mean serum ?-hCG was much higher in the case group than that in the control group (p<0.001). The mean serum ?-hCG was the highest in severe preeclampsia and the lowest in the control group, while that in mild preeclampsia lie in between the two (p<0.001). The serum ?-hCG exhibits a significantly linear correlation with systolic and diastolic blood pressures (p<0.001 respectively). Conclusion: There was a significant difference between the ?-hCG level in the preeclamptic women compared to the normotensive pregnant women and the severity of preeclamsia increases with further rise of ?-hCG level. DOI: http://dx.doi.org/10.3329/jdmc.v23i1.22701 J Dhaka Medical College, Vol. 23, No.1, April, 2014, Page 89-93

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The Relationship of Smoking, Alcohol, and Drugs Consumption on the Quality of Semen Parameters in Males referring to the Infertility Center of Yazd in 2016

The Journal of Tolooebehdasht ◽

10.18502/tbj.v17i5.313 ◽

2019 ◽

Author(s):

Marjan Shafabakhsh ◽

HosseinAli Sadeghian ◽

Mohammad Hassan Ehramposh ◽

Abas Aflatonian ◽

Arefeh Dehghani ◽

...

Keyword(s):

Male Infertility ◽

Semen Parameters ◽

Treatment Center ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Significant Difference ◽

The Mean ◽

And Control ◽

Test Result

Introduction: Male infertility has increased during recent years in countries of the world including Iran. Among many factors that influence the infertility, smoking, alcohol, and addictive drugs were investigated in this study. Methods: This case-control retrospective study was conducted on 600 male patients consisting of 300 individuals with natural semen and 300 people with defective semen. The first step in investigation of infertility in each couple was to conduct a spermogram to detect the male infertility. The case group included men who referred to the Yazd Infertility Treatment Center and had at least one abnormal test result among considering the parameters of semen test. The control group included the men who referred to the same center and had normal semen test result. The data collection tools were a researcher-made checklist based on the parameters required for the study with epidemiological and observational design, as well as the semen analysis test. Results: The age mean in the case and control groups were 34.71 and 33.82 years with the standard deviation of ± 7.3 and ± 6.41, respectively. The means of marriage age in the case and control groups were 26.28 and 26.28 ±5.3 years, respectively. The mean duration of infertility in the case group was 6.30 ± 4.60 and in the control group was 5.15± 3.88. No significant difference was observed between the mean age (P = 0.084) and marital age (P = 0.448) in two groups, but the mean duration of infertility (P <0.001) was significantly different between the two groups .The prevalence of smoking was significantly different between the two groups (P = 0.032). Considering the daily frequency of smoking, the highest rate was 11-20 cigarettes in both groups (case group 21.7% and control group 15.3%). The P value was set at 0.018. Conclusion: In this study, smoking and consumption of alcohol were not considered as the predictive factors of semen quality, but consumption of drugs and the age of starting consumption of drugs had a significant relationship with the semen disorders.

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