The population structure of Beijing family of Mycobacterium tuberculosis in Western Siberia

ABSTRACT We compared the population structure and drug resistance patterns of the Mycobacterium tuberculosis strains currently circulating in the Beijing area of China. One hundred thirteen of 123 strains belonged to the Beijing family genotypes defined by spoligotyping. The Beijing genotype strains were further subdivided into old and modern sublineages on the basis of NTF locus analysis. A stronger association with resistance to the more recently introduced antituberculosis drugs has been observed for old versus modern strains of the Beijing genotype, suggesting that its different sublineages may differ in their mechanisms of adaptation to drug selective pressure.

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Phylogeographical particularity of the Mycobacterium tuberculosis Beijing family in South Korea based on international comparison with surrounding countries

Journal of Medical Microbiology ◽

10.1099/jmm.0.022103-0 ◽

2010 ◽

Vol 59 (10) ◽

pp. 1191-1197 ◽

Cited By ~ 34

Author(s):

Hee Yoon Kang ◽

Takayuki Wada ◽

Tomotada Iwamoto ◽

Shinji Maeda ◽

Yoshiro Murase ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Population Structure ◽

Spanning Tree ◽

Minimum Spanning Tree ◽

Variable Number ◽

Genomic Region ◽

Beijing Family ◽

Eastern Asia ◽

The Republic ◽

Far Eastern

To understand the domestic population structure of Mycobacterium tuberculosis clinical isolates in the Republic of Korea, we genotypically analysed 80 isolates obtained from various geographical origins in the country. Of these, 64 (80.0 %) isolates were identified as Beijing family strains. It is particularly interesting that their phylogenetic classification, based on the ancient/modern separation and the presence/absence of the genomic region RD181, revealed a majority of the ancient (RD181+) subfamily in the population. The 15 loci of variable number of tandem repeat(s) of mycobacterial interspersed repetitive units (15-MIRU-VNTR) were also analysed. Combination with the previous VNTR data reported from surrounding countries revealed that the topology of the minimum spanning tree was linked tightly not to the geographical origins of the patients but to the phylogenetic characteristics of the isolates. These results show that the phylogeographical distribution of the M. tuberculosis Beijing family around far-eastern Asia could be estimated using international accumulation and comparison of VNTR genotyping data.

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Profiling the immune response to Mycobacterium tuberculosis Beijing family infection: a perspective from the transcriptome

Virulence ◽

10.1080/21505594.2021.1936432 ◽

2021 ◽

Vol 12 (1) ◽

pp. 1689-1704

Author(s):

Cerezo-Cortés María Irene ◽

Rodríguez-Castillo Juan Germán ◽

López-Leal Gamaliel ◽

Mata-Espinosa Dulce Adriana ◽

Bini Estela Isabel ◽

...

Keyword(s):

Immune Response ◽

Mycobacterium Tuberculosis ◽

Beijing Family

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Complete Genome Sequences of Mycobacterium tuberculosis Strains CCDC5079 and CCDC5080, Which Belong to the Beijing Family

Journal of Bacteriology ◽

10.1128/jb.05452-11 ◽

2011 ◽

Vol 193 (19) ◽

pp. 5591-5592 ◽

Cited By ~ 28

Author(s):

Y. Zhang ◽

C. Chen ◽

J. Liu ◽

H. Deng ◽

A. Pan ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Complete Genome ◽

Beijing Family ◽

Genome Sequences

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Tuberculose Multirresistente por Estirpes da Família Beijing, em Doentes de Lisboa, Portugal: Estudo Preliminar

Acta Médica Portuguesa ◽

10.20344/amp.7882 ◽

2017 ◽

Vol 30 (3) ◽

pp. 175 ◽

Cited By ~ 2

Author(s):

Fernando Maltez ◽

Teresa Martins ◽

Diana Póvoas ◽

João Cabo ◽

Helena Peres ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Past History ◽

Multidrug Resistant ◽

Resistance Pattern ◽

Drug Resistant ◽

Beijing Family ◽

Resistant Tuberculosis ◽

Extensively Drug Resistant ◽

Number Of Patients ◽

Drug Resistant Strains

Introduction: Beijing family strains of Mycobacterium tuberculosis are associated with multidrug-resistance. Although strains of the Lisboa family are the most common among multidrug-resistant and extensively drug-resistant patients in the region, several studies have reported the presence of the Beijing family. However, the features of patients from whom they were isolated, are not yet known.Material and Methods: Retrospective study involving 104 multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis, from the same number of patients, isolated and genotyped between 1993 and 2015 in Lisbon. We assessed the prevalence of strains of both families and the epidemiologic and clinical features of those infected with Beijing family strains.Results: Seventy-four strains (71.2%) belonged to the Lisboa family, 25 (24.0%) showed a unique genotypic pattern and five (4.8%) belonged to the Beijing family, the latter identified after 2009. Those infected with Beijing family strains were angolan (n = 1), ukrainian (n = 2) and portuguese (n = 2), mainly young-aged and, four of five immunocompetent and with no past history of tuberculosis. All had multidrug-resistant tuberculosis. We did not find any distinctive clinical or radiological features, neither a predominant resistance pattern. Cure rate was high (four patients).Discussion: Although the number of infected patients with Beijing strains was small, it suggests an important proportion of primary tuberculosis, a potential for transmission in the community but also a better clinical outcome when compared to other reported strains, such as W-Beijing and Lisboa.Conclusion: Although Lisboa family strains account for most of the multidrug and extensively drug-resistant tuberculosis cases in Lisbon area, Beijing strains are transmitted in the city and might change the local characteristics of the epidemics.

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Genotypes of Mycobacterium tuberculosis isolates circulating in Shaanxi Province, China

PLoS ONE ◽

10.1371/journal.pone.0242971 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0242971

Author(s):

Yan Li ◽

Yu Pang ◽

Tianhua Zhang ◽

Xiaoping Xian ◽

Jian Yang ◽

...

Keyword(s):

Genetic Diversity ◽

Drug Resistance ◽

Mycobacterium Tuberculosis ◽

Multidrug Resistant ◽

Shaanxi Province ◽

Drug Resistant ◽

Beijing Family ◽

Tuberculosis Patients ◽

Smear Positive Pulmonary Tuberculosis ◽

Discriminatory Index

Objectives The prevalence of drug-resistant TB in Shaanxi Province is higher than other areas. This study was aimed to investigate the genetic diversity and epidemiology of Mycobacterium tuberculosis clinical strains in Shaanxi Province, China. Methods From January to December 2016, a total of 298 Mycobacterium tuberculosis clinical isolates from smear-positive pulmonary tuberculosis patients were genotyped by Mcspoligotyping and 15-locus VNTR. Results We found that the Beijing family strains was the most prominent family(81.54%, 243/298). Other family strains included T family(9.06%, 27/298), U family(0.67%, 2/298), LAM9 family(0.34%, 1/298) and Manu family(0.34%, 1/298). The rates of multidrug-resistant (MDR) M.Tuberculosis, age, type of case and education between Beijing and non-Beijing family strains were not statistically different, while the distribution in the three different regions among these was statistically significant. VNTR results showed that strains were classified into 280 genotypes, and 33 (11.07%) strains could be grouped into 14 clusters. 11 of the 15-VNTR loci were highly or moderately discriminative according to the Hunter-Gaston discriminatory index. Conclusions We concluded that the Beijing family genotype was the most prevalent genotype and 15-locus VNTR typing might be suitable for genotyping of M. tuberculosis in Shaanxi Province. There was less association between Beijing family genotypes and drug resistance in our study area.

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Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

Journal of Medical Microbiology ◽

10.1099/jmm.0.47005-0 ◽

2007 ◽

Vol 56 (9) ◽

pp. 1219-1223 ◽

Cited By ~ 15

Author(s):

Hui Han ◽

Fang Wang ◽

Yong Xiao ◽

Yi Ren ◽

Yanjie Chao ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Molecular Epidemiology ◽

High Throughput ◽

Discriminatory Power ◽

Fast Method ◽

Beijing Family ◽

Wuhan City ◽

Preliminary Screening ◽

Discriminatory Index

Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis, and may represent a feasible approach to study M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating M. tuberculosis strains, isolates from 105 patients in Wuhan City, China, were genotyped by this method as compared to spoligotyping. MIRU typing identified 55 types that defined 21 clusters and 34 unique isolates. The discriminatory power was high [Hunter–Gaston discriminatory index (HGDI), 0.97]. Spoligotyping showed that 86 (81.9 %) of 105 isolates belonged to the Beijing family genotype. For Beijing family and non-Beijing strains, the discriminatory power of MIRU was high (HGDI, 0.95 and 0.98, respectively). Among the alleles of the MIRU loci for the Beijing family, only locus 26 was highly discriminative, but for non-Beijing strains, loci 10, 16 and 26 were highly discriminative. MIRU typing is a simple and fast method which may be used for preliminary screening of M. tuberculosis isolates in China.

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Diverse Clinical Isolates of Mycobacterium tuberculosis Develop Macrophage-Induced Rifampin Tolerance

The Journal of Infectious Diseases ◽

10.1093/infdis/jiy710 ◽

2019 ◽

Vol 219 (10) ◽

pp. 1554-1558 ◽

Cited By ~ 2

Author(s):

Kristin N Adams ◽

Amit Kumar Verma ◽

Radha Gopalaswamy ◽

Harresh Adikesavalu ◽

Dinesh Kumar Singhal ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Clinical Isolates ◽

Beijing Family ◽

Lineage 2 ◽

Induced Tolerance ◽

Multiple Antibiotics

Mycobacterium tuberculosis develops tolerance to multiple antibiotics when residing in host macrophages. We demonstrate that macrophage-induced tolerance to rifampin is common across major lineages of M. tuberculosis, except for Beijing-family lineage 2 strains.

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Population structure of drug-resistant Mycobacterium tuberculosis in Central Asia

BMC Infectious Diseases ◽

10.1186/s12879-019-4480-7 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 4

Author(s):

Anna Engström ◽

Uladzimir Antonenka ◽

Abdylat Kadyrov ◽

Gulmira Kalmambetova ◽

Katharina Kranzer ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Population Structure ◽

Central Asia ◽

Tandem Repeats ◽

Relative Size ◽

Multidrug Resistant ◽

Variable Number ◽

Health Concern ◽

Drug Resistant ◽

Recent Emergence

Abstract Background Drug-resistant tuberculosis (TB) is a major public health concern threathing the success of TB control efforts, and this is particularily problematic in Central Asia. Here, we present the first analysis of the population structure of Mycobacterium tuberculosis complex isolates in the Central Asian republics Uzbekistan, Tajikistan, and Kyrgyzstan. Methods The study set consisted of 607 isolates with 235 from Uzbekistan, 206 from Tajikistan, and 166 from Kyrgyzstan. 24-loci MIRU-VNTR (Mycobacterial Interspersed Repetitive Units - Variable Number of Tandem Repeats) typing and spoligotyping were combined for genotyping. In addition, phenotypic drug suceptibility was performed. Results The population structure mainly comprises strains of the Beijing lineage (411/607). 349 of the 411 Beijing isolates formed clusters, compared to only 33 of the 196 isolates from other clades. Beijing 94–32 (n = 145) and 100–32 (n = 70) formed the largest clusters. Beijing isolates were more frequently multidrug-resistant, pre-extensively resistant (pre-XDR)- or XDR-TB than other genotypes. Conclusions Beijing clusters 94–32 and 100–32 are the dominant MTB genotypes in Central Asia. The relative size of 100–32 compared to previous studies in Kazakhstan and its unequal geographic distribution support the hypothesis of its more recent emergence in Central Asia. The data also demonstrate that clonal spread of resistant TB strains, particularly of the Beijing lineage, is a root of the so far uncontroled MDR-TB epidemic in Central Asia.

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