INFLUENCE OF THE ACUTE INFLAMMATION AND ENDOTHELIAL DYSFUNCTION ON THE RETINAL VEIN OCCLUSION FORMATION AFTER CARDIO-SURGICAL INTERFERENCES WITH THE USE OF CARDIOPULMONARY BYPASS

Acute inflammation and endothelial dysfunction (EDF) are typical pathological processes, which determine the development of retinal vein occlusion (RVO) during cardio-surgery with the use of cardiopulmonary bypass (CB), but the connection of seromarkers according to the terms of occlusion appearance remains undefined. The aim – to determine the influence of the acute inflammation and EDF for RVO formation after cardio-surgical interferences with the use of CB according to the terms of occlusion appearance. Material and methods. There were selected for the research the data of 137 eyes (126 patients, the main group) with RVO after the surgery with CB. The comparison group contains the data about examination of 86 eyes (43 patients), who had not any occlusion during all term of examination. The control group consisted of 10 eyes (5 patients) without occlusion, which were examined before surgery. An ophthalmologist 2, 7, 30, 60, 90 and 180 days after cardio-surgical interference, examined patients. The content of IL-6, IL-8 and VE-cadherin in blood serum was determined by immunoenzyme technique (Bender Medsystems, Austria). Statistical data processing was performed with the use of Statistica 10 program (StatSoft, Inc., USA), regression analysis – with the use of the program package GLZ. Results. The conduction of cardio-surgeries with the use of CB caused an increase of the interleukins content in the early period (IL-6 on the 2nd and 7th days, and IL-8 up to 30 days), while the content of VE-cadherin (VE-C) was slightly increased during almost all period of monitoring. With the availability of RVO, the content of IL-6 during all terms of occlusion appearance was significantly higher, the content of IL-6 was up to 30 days, and the content of VE-C in a greater degree was after the 7th day. The regression analysis showed that after 1-2 days RVO appearance was directly related with the content of IL-6 and IL-8 in the blood, on the 3rd and 7th days – only with the content of IL-8, on the 8th and 30th days – with the content of all markers, and then with the content of IL-6 and VE-C. The accuracy of the prediction of the presence or absence of RVO at the appropriate period according to the calculated regression model is at least 78 % (p <0.001), what proves the influence of markers on the development of RVO. Conclusions. The undertaken study shows the meaning of the acute inflammation and EDF by appearance of RVO with the use of CB, what justifies the application of the preventive measures - at the early stages the restriction of activity of the inflammatory process, at the later stages – prevention of EDF development.

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Retinal Vein Occlusion: A Form of Local Venous Thrombosis or a Result of Systemic Endothelial Dysfunction

10.21203/rs.3.rs-258297/v1 ◽

2021 ◽

Author(s):

Erdinç Bozkurt ◽

Turkhun Cetin ◽

Ibrahim Rencuzogulları

Keyword(s):

Endothelial Dysfunction ◽

Carotid Artery ◽

Endothelial Function ◽

Retinal Vein Occlusion ◽

Carotid Artery Disease ◽

Hematological Parameters ◽

Intima Media Thickness ◽

Control Group ◽

Vein Occlusion ◽

Significant Difference

Abstract Background: To determine the condition of systemic endothelial function and carotid intima media thickness (CIMT) in patients with retinal vein occlusion (RVO) and to identify the risk factors associated with the disease.Methods: Seventy-six patients who presented to the clinic with the diagnosis of RVO and 76 age- and gender-matched healthy individuals without a RVO history were included in the study. The patients' vision levels and central macular thickness were measures, and diabetes, hypertension, hyperlipidemia, carotid artery disease, body mass index and smoking histories were recorded. The endothelial function levels of the patients, pulse wave velocity (PWV), and CIMT were measured. Serum hematological parameters were evaluated.Results: The most important risk factor in patients with RVO was found to be hypertension (OR 1.455, 95% CI 1.981-9.272; p=0.001) compared to the control group, and diabetes (OR 0.460, 95% CI 1.981-9.272; p=0.001) and hyperlipidemia (OR 0.124, 95% CI 0.371-3.456; p=0.828) were significantly more common among the patients.There was a statistically significant difference between the RVO and control groups in terms of flow-mediated dilation (OR 0.050, 95% CI 0.020-0.080; p =0.001), PWV (OR 0.392, 95% CI 0.271-0.513; p<0.001), and the CIMT of both sides (OR 2.434, 95% CI 1.801-3.055 for the right CIMT and OR 2.284, 95% CI 1.646-2.922 for the left CIMT) (p<0.001 for all).Conclusion: Considering that RVO is associated with systemic endothelial dysfunction and may also be accompanied by carotid artery stenosis, we think that additional systemic diseases should be questioned in this patient group.

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Evaluation of Systemic Endothelial Dysfunction in Patients with Retinal Vein Occlusion

10.21203/rs.3.rs-341111/v1 ◽

2021 ◽

Author(s):

Erdinc Bozkurt ◽

Turkhun Cetin ◽

Ibrahim Rencuzogulları

Keyword(s):

Endothelial Dysfunction ◽

Carotid Artery ◽

Endothelial Function ◽

Retinal Vein Occlusion ◽

Carotid Artery Disease ◽

Hematological Parameters ◽

Intima Media Thickness ◽

Control Group ◽

Vein Occlusion ◽

Significant Difference

Abstract Purpose: To determine the condition of systemic endothelial function and carotid intima media thickness (CIMT) in patients with retinal vein occlusion (RVO) and to identify the risk factors associated with the disease.Material-Method: Seventy-six patients who presented to the clinic with the diagnosis of RVO and 76 age- and gender-matched healthy individuals without a RVO history were included in the study. The patients' vision levels and central macular thickness were measures, and diabetes, hypertension, hyperlipidemia, carotid artery disease, body mass index and smoking histories were recorded. The endothelial function levels of the patients, pulse wave velocity (PWV), and CIMT were measured. Serum hematological parameters were evaluated.Results: The most important risk factor in patients with RVO was found to be hypertension (OR 1.455, 95% CI 1.981-9.272; p=0.001) compared to the control group, and diabetes (OR 0.460, 95% CI 1.981-9.272; p=0.001) and hyperlipidemia (OR 0.124, 95% CI 0.371-3.456; p=0.828) were significantly more common among the patients.There was a statistically significant difference between the RVO and control groups in terms of flow-mediated dilation (OR 0.050, 95% CI 0.020-0.080; p =0.001), PWV (OR 0.392, 95% CI 0.271-0.513; p<0.001), and the CIMT of both sides (OR 2.434, 95% CI 1.801-3.055 for the right CIMT and OR 2.284, 95% CI 1.646-2.922 for the left CIMT) (p<0.001 for all).Conclusion: Considering that RVO is associated with systemic endothelial dysfunction and may also be accompanied by carotid artery stenosis, we think that additional systemic diseases should be questioned in this patient group.

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Increased risk of cancer in patients with retinal vein occlusion: a 12-year nationwide cohort study

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2020-316947 ◽

2020 ◽

pp. bjophthalmol-2020-316947

Author(s):

Min Seok Kim ◽

Joon Hee Cho ◽

Seong Jun Byun ◽

Chang-Mo Oh ◽

Kyu Hyung Park ◽

...

Keyword(s):

Retinal Vein Occlusion ◽

Cox Regression ◽

Subsequent Development ◽

Population Based ◽

Control Group ◽

Time Varying ◽

Vein Occlusion ◽

Increased Risk ◽

Risk Of Cancer ◽

Subsequent Cancer

AimsTo investigate the association between incident retinal vein occlusion (RVO) and the subsequent development of cancer.MethodsIn this nationwide population-based retrospective study using 2002–2013 National Health Insurance Service database which covers the entire South Korean population, 186 701 incident RVO patients and their 1:1 propensity-score matched controls were included. We defined the fixed cohort from January 1st, 2004 to December 31st, 2013; the cohort included patients who suffered incident RVO after entering the cohort and their matched controls, and excluded patients having any cancer history before entering the cohort. The association of RVO and cancer was assessed by time-varying covariate Cox regression models; Model 1 included RVO as a time-varying covariate, Model 2 included Model 1 plus demographic information and Model 3 included Model 2 and comorbidities.ResultsRVO was associated with an increased risk of subsequent cancer (HR=1.29; 95% CI, 1.26–1.31 in Model 1), which was consistent in Models 2 and 3. The incidence rate of overall cancer during the study period was 25.55 (95% CI, 25.19–25.91) per 1000 person-years in the RVO group and 18.62 (95% CI, 18.46–18.79) per 1000 person-years in the control group. In the subgroup analysis, haematological malignancies showed the highest association with RVO (HR=1.65; 95% CI, 1.49–1.83).ConclusionPatients with RVO have an increased risk of subsequent cancer development even after adjusting for demographic factors and comorbidities. Further study is warranted to elucidate these associations to provide proper recommendations for RVO patients regarding the cancer screening.

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Central retinal vein occlusion associated with platelet activation

Therapeutic Advances in Ophthalmology ◽

10.1177/2515841419864844 ◽

2019 ◽

Vol 11 ◽

pp. 251584141986484 ◽

Cited By ~ 1

Author(s):

Mehmet Citirik

Keyword(s):

Retinal Vein Occlusion ◽

Platelet Activation ◽

Central Retinal Vein Occlusion ◽

Mean Platelet Volume ◽

Control Group ◽

Platelet Distribution Width ◽

Platelet Volume ◽

Distribution Width ◽

Vein Occlusion ◽

Central Retinal Vein

Purpose:The aim of this article is to determine and compare the platelet activation by three main platelet activation parameters: mean platelet volume, platelet distribution width, and plateletcrit in patients with central retinal vein occlusion and control subjects.Methods:This study included 30 patients with nonischemic central retinal vein occlusion and 30 control subjects. The levels of mean platelet volume, platelet distribution width, and plateletcrit were measured in all groups.Results:The mean serum level of mean platelet volume was 10.01 ± 0.89 fl in central retinal vein occlusion group and 8.74 ± 1.45 fl in control group. The mean serum level of platelet distribution width was 14.31 ± 1.49% and 11.65 ± 1.81% in central retinal vein occlusion group and control group, respectively. Mean serum plateletcrit value was 0.27 ± 0.07% in central retinal vein occlusion group and 0.23 ± 0.07% in control group. Mean platelet volume, platelet distribution width, and plateletcrit levels were significantly higher in central retinal vein occlusion patients than controls ( p < 0.05).Conclusion:Subclinical platelet activation reflected by mean platelet volume, platelet distribution width, and plateletcrit may have an impact on the genesis of vessel occlusion in central retinal vein occlusion. The results may be important for the clinical management of patients with central retinal vein occlusion.

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Fibrinolytic Response in Retinal vein Occlusion and Diabetic Retinopathy

10.1055/s-0039-1680778 ◽

1977 ◽

Author(s):

V. De Rosa ◽

M. Pe-duzzi ◽

F. Montanari ◽

S. Coccheri

Keyword(s):

Diabetic Retinopathy ◽

Retinal Vein Occlusion ◽

Venous Stasis ◽

Control Group ◽

Poor Responders ◽

Vein Occlusion ◽

Lysis Time ◽

Frequent Finding ◽

Euglobulin Lysis Time ◽

Sustained Increase

Coagulation and fibrinolysis studies were performed in 40 patients during the second week after occurrence of Retinal Vein Occlusion (RVO), in 35 patients with Diabetic Retinopathy of various degree and no thrombosis (DR), and in an age-sex matched control group.Euglobulin Lysis Time was markedly prolonged in 30% of RVO and in 44% of DR patients. Poor responders to venous stasis ranked around 35% in both groups. Urokinase inhibition was increased in one third of RVO and in about two thirds of DR patients. These and other findings like increase of Factor VIII or platelet aggregation were only seldom associated in the same subjects.Decreased plasma or tissue fibrinolysis is a frequent finding both in RVO and in DR and trials with drugs inducing a sustained increase of fibrinolytic activity are therefore justified in these conditions.

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Neutrophil/Lymphocyte Ratio and Platelet/Lymphocyte Ratio in Branch Retinal Vein Occlusion

Journal of Ophthalmology ◽

10.1155/2019/6043612 ◽

2019 ◽

Vol 2019 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Dan-dan Zhu ◽

Xun Liu

Keyword(s):

Retinal Vein Occlusion ◽

Branch Retinal Vein Occlusion ◽

Roc Analysis ◽

Control Group ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio ◽

Platelet Lymphocyte Ratio ◽

Vein Occlusion ◽

The Mean ◽

Age And Sex

Purpose. To evaluate the neutrophil/lymphocyte ratio (NLR) and the platelet/lymphocyte ratio(PLR) value in the development of branch retinal vein occlusion (BRVO)patients. Methods. 81 patients with BRVO and 81 age and sex-matched subjects were recruited as the control group. The BRVO diagnosis was confirmed under comprehensive ophthalmologic examinations. NLR and PLR parameters obtained from peripheral blood were recorded. Results. Both the mean NLR and PLR was significantly higher in the BRVO group compared with the control group (p < 0.001). In ROC analysis, the AUC for NLR was 0.82, and NLR of >2.48 predicted BRVO with a sensitivity of 58% and specificity of 98%. The AUC for PLR was 0.78, and PLR of >110.2 predicted BRVO with a sensitivity of 72% and specificity of 72%. Conclusion. The current study demonstrated that BRVO patients had increased NLR and PLR levels compared with control subjects. The NLR and PLR may be used as independent predictors for identifying risk for the development of BRVO.

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The new complex method of treatment central retinal veinand branch of retinal vein occlusion

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2008-1-99-101 ◽

2008 ◽

Vol 7 (1) ◽

pp. 99-101

Author(s):

D. V. Petrachkov

Keyword(s):

Retinal Vein Occlusion ◽

Treatment Method ◽

Suggested Method ◽

Control Group ◽

Complex Method ◽

Vein Occlusion ◽

New Treatment ◽

To Receive ◽

Medical Effect ◽

Central Retinal Vein

The new treatment method of central retinal vein and branch of retinal vein occlusion, consisting of epiretinal injection of gemasa combined with standard pharmacotherapy was developed. Clinical researches were carried out by the group of 42 patients with central retinal vein and branch of retinal vein occlusion. The control group was appeared by 62 patients. Based on findings we can draw a conclusion, that the application of a suggested method allows to receive more stabile medical effect and reduce the number of complications.

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Significant elevation of aqueous endothelin-1 in central retinal vein occlusion

PLoS ONE ◽

10.1371/journal.pone.0252530 ◽

2021 ◽

Vol 16 (6) ◽

pp. e0252530

Author(s):

Hae Min Kang ◽

Md. Hasanuzzaman ◽

So Won Kim ◽

Hyoung Jun Koh ◽

Sung Chul Lee

Keyword(s):

Retinal Vein Occlusion ◽

Aqueous Humor ◽

Central Retinal Vein Occlusion ◽

Intravitreal Bevacizumab ◽

Control Group ◽

Endothelin 1 ◽

Intravitreal Bevacizumab Injection ◽

Vein Occlusion ◽

The Mean ◽

Central Retinal Vein

Purpose To investigate aqueous humor concentrations of endothelin-1 (ET-1) in patients with central retinal vein occlusion (CRVO) compared with patients with branch retinal vein occlusion (BRVO) and a normal control group. Methods A total 80 subjects were included in this prospective study, including 15 patients with CRVO, 20 patients with BRVO, and 45 patients who underwent cataract surgery and had no concomitant ocular disease. Aqueous humor levels of ET-1 were obtained before intravitreal bevacizumab injection (IVB) and after 1 month. Results At baseline, the mean aqueous ET-1 level was 12.7±3.6 pg/mL in the CRVO group, 8.0±2.3 pg/mL in the BRVO group, and 2.0±0.9 pg/mL in the control group (P<0.001). After IVB, the mean aqueous level of ET-1 was 3.4±1.9 pg/mL (0.5–6.9 pg/mL) in the CRVO group and 1.8±1.0 pg/mL (0.3–3.2 pg/mL) in the BRVO group (P = 0.008). The mean aqueous ET-1 level was significantly reduced in both the patients with CRVO and those with BRVO (P<0.001). Conclusion The mean aqueous humor ET-1 level was significant higher in the patients with CRVO than those with BRVO and in the control group. After IVB, the mean level was significantly reduced in both the patients with CRVO and those with BRVO.

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Role of the Choroidal Vascularity Index in Branch Retinal Vein Occlusion with Macular Edema

10.21203/rs.3.rs-136979/v1 ◽

2021 ◽

Author(s):

Bo-Een Hwang ◽

Mirinae Kim ◽

Young-Hoon Park

Keyword(s):

Regression Analysis ◽

Macular Edema ◽

Retinal Vein Occlusion ◽

Branch Retinal Vein Occlusion ◽

Prognostic Biomarker ◽

Strong Association ◽

Disease Onset ◽

Swept Source ◽

Vein Occlusion ◽

Endothelial Growth Factor

Abstract The purpose of this study was to assess choroidal vasculature changes in eyes with branch retinal vein occlusion (BRVO) and macular edema (ME) using the choroidal vascularity index (CVI) and evaluate the effectiveness of CVI as a prognostic biomarker. 35 patients (70 eyes) with BRVO and ME were analyzed retrospectively. Luminal and stromal areas in choroids of swept-source optical coherence tomography were calculated using the image binarization technique. The CVI was calculated as the ratio of the luminal to total choroidal area. The CVI of BRVO and ME eyes were compared with that of the unaffected fellow and post anti-vascular endothelial growth factor (VEGF) injected eyes. A regression analysis was performed on the choroidal parameters and logMAR visual acuity (VA) two years post disease onset. The CVI of BRVO and ME eyes was significantly lower than the fellow and post-injected eyes (p<0.05). The regression analysis showed a strong association between two years after logMAR VA and the CVI of fellow eyes (R2=0.433, p<0.001). No remarkable R2 values were observed in the CVI and subfoveal choroidal thickness of BRVO and ME eyes (R2=0.189, 0.155, respectively, p<0.05). Reduced CVI in BRVO and ME suggests that retinal ischemia and choroidal vascular changes might be closely related. The fellow eye CVI could be a useful supplementary prognostic biomarker.

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Targeting of cell-free DNA by DNase I diminishes endothelial dysfunction and inflammation in a rat model of cardiopulmonary bypass

Scientific Reports ◽

10.1038/s41598-019-55863-8 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Carolyn Weber ◽

Alexander Jenke ◽

Vasilena Chobanova ◽

Mariam Yazdanyar ◽

Agunda Chekhoeva ◽

...

Keyword(s):

Cardiopulmonary Bypass ◽

Endothelial Dysfunction ◽

Adhesion Molecules ◽

Therapeutic Strategy ◽

Dnase I ◽

Intercellular Adhesion Molecule ◽

Control Group ◽

Cell Free Dna ◽

Free Dna ◽

Group 3

AbstractThe use of cardiopulmonary bypass (CPB) results in the activation of leukocytes, release of neutrophil extracellular traps (NETs) and severe inflammation. We hypothesize that targeting of circulating cell-free DNA (cfDNA) by DNases might represent a feasible therapeutic strategy to limit CPB-associated side effects. Male Wistar rats (n = 24) underwent CPB with deep hypothermic circulatory arrest (DHCA) and were divided into 3 groups: control (group 1), one i.v. bolus DNase I before CPB start (group 2) and a second DNase I dose before reperfusion (group 3). We found a positive correlation between plasma cfDNA/NETs levels and compromised endothelial vasorelaxation after CPB. DNase I administration significantly diminished plasma cfDNA/NETs levels. Further, a dose-dependent improvement in endothelial function accompanied by significant reduction of circulating intercellular adhesion molecule (ICAM)-1 was observed. Rats of group 3 had significantly reduced plasma IL-6 levels and downregulated expression of adhesion molecules resulting in impaired leukocyte extravasation and reduced MPO activity in lungs. Mechanistically, digestion of NETs by DNase I significantly diminished NETs-dependent upregulation of adhesion molecules in human endothelial cells. Altogether, systemic DNase I administration during CPB efficiently reduced cfDNA/NETs-mediated endothelial dysfunction and inflammation and might represents a promising therapeutic strategy for clinical practice.

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